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Citations for Addgene :
1 - 50 of 873 citations for Recombinant Human Long Arg3 Insulin like Growth Factor I since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: Recombinant human GST-CK2α’ (Addgene #27084) and recombinant human GST-CK2α (Addgene #27083 ...
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bioRxiv - Biochemistry 2024Quote: ... and recombinant human GST-CK2α (Addgene #27083) enzymes were produced in-house as previously described [30] ...
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bioRxiv - Plant Biology 2024Quote: The long CaMV35S long promoter (Addgene #50267) and CaMV 35S terminator (Addgene #50337 ...
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bioRxiv - Microbiology 2021Quote: ... The recombinant plasmid containing human ACE2 gene (Addgene #1786) was transfected into HEK293T cells using Lipofectamine 2000 Transfection Reagent (Thermo Fisher ...
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bioRxiv - Neuroscience 2023Quote: Full-length recombinant human WT α-syn (Addgene #213498), α-syn 1-95 (Addgene #213499) ...
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bioRxiv - Cancer Biology 2023Quote: The human Insulin Receptor cDNA was a gift from Frederick Stanley (Addgene plasmid # 24049 ...
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bioRxiv - Molecular Biology 2023Quote: ... was inserted into a pcDNA3.1-Hygro(-)-like with a C-terminal HRV 3C cut site and human Fc tag amplified from Addgene plasmid 145164 using standard cloning techniques ...
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bioRxiv - Biochemistry 2022Quote: ... The recombinant human PRKACA cDNA was obtained from Addgene (Plasmid #23495) and cloned into between BamHI and KpnI in pcDNA5 vector (Invitrogen) ...
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bioRxiv - Neuroscience 2023Quote: A 2.2 kb BamHI-SalI cDNA fragment of the long form of human PREPL (PREPLL) was cloned into pLenti-GFP (Addgene) digested with the same restriction enzymes ...
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bioRxiv - Cell Biology 2021Quote: ... GFP-Intersectin Long (Addgene plasmid # 47395) and GFP-CLCb (EED/QQN ...
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bioRxiv - Cancer Biology 2023Quote: The human Insulin Receptor cDNA was a gift from Frederick Stanley (Addgene plasmid # 24049; http://n2t.net/addgene:24049; RRID:Addgene_24049). This construct (hIR ...
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bioRxiv - Biochemistry 2021Quote: ... N-terminal His6 plus small ubiquitin-like modifier (SUMO; RRID:Addgene_29711); or N-terminal His6 plus green fluorescent protein (GFP ...
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bioRxiv - Biophysics 2023Quote: ... The reporter plasmids were subcloned using the vector backbone of pGL410_INS421 which has a human insulin promotor regulating firefly luciferase expression (a gift from Kevin Ferreri Addgene plasmid #49057 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... A human elongation factor 1 (EF1) promoter was introduced using a Gateway att4-att1 Entry clone (Addgene, Watertown, MA, #162920). Final expression clones were verified by restriction analysis and maxiprep DNA was prepared using the Qiaprep Maxiprep kit (Qiagen ...
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bioRxiv - Molecular Biology 2023Quote: - recombinant pGEX-4T-3-P53: The human-p53 cDNA was previously cloned in the EcoRI site of pGEX-4T3 (Addgene_79149) under the lac-trp hybrid promoter that is inducible by IPTG ...
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bioRxiv - Synthetic Biology 2022Quote: ... Insulin in pX330S-4 (Plasmid #58780, Addgene) and Glut2 in pX330S-5 (Plasmid #58781 ...
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bioRxiv - Neuroscience 2023Quote: ... we utilized PiggyBac integration technology to express PB-tet-NGN2 (Addgene 172115; “cortical-like” neurons) and PB-tet-hNIL (Addgene 172113 ...
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bioRxiv - Genetics 2020Quote: ... which was a kind gift from Ji-Long Liu (Addgene plasmid 49330). This plasmid has been shown to work well in Ae ...
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bioRxiv - Molecular Biology 2023Quote: FLAG-TAL1-short/long were cloned to MIGR1 vector (Addgene, plasmid#27490). HEK293T cells were co-transfected with the viral backbone vector pCMV-VSVG and pCL-Eco packaging vectors using polyethylenimine (PEI ...
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bioRxiv - Cell Biology 2023Quote: Plasmids encoding recombinant WT (RRID:Addgene_92100) and M880A mutant human GST-TAF3-PHD were kindly provided by Albert Jeltsch (Kungulovski et al. ...
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bioRxiv - Genomics 2021Quote: ... shRNA-specific plasmid together with lentiviral packaging plasmids like VSVG (a gift from Bob Weinberg, Addgene #8454), and PAX2 ...
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bioRxiv - Molecular Biology 2020Quote: ... and pAc from pAc-sgRNA-Cas930 (a gift from Ji-Long Liu; Addgene plasmid #49330 ...
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bioRxiv - Genomics 2023Quote: pBabe-puro LEF1 (the long isoform) was a gift from Joan Massague (Addgene plasmid # 27023 ...
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bioRxiv - Bioengineering 2022Quote: ... The T-cell factor/lymphoid enhancer factor (TCF/LEF) luciferase reporter SuperTopFlash (STF) and the control pRL-SV40 Renilla luciferase constructs (Addgene) were used for Wnt/β-catenin-responsive reporter assays ...
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Comprehensive fitness landscape of SARS-CoV-2 Mpro reveals insights into viral resistance mechanismsbioRxiv - Molecular Biology 2022Quote: ... The LexA_ER_B112 transcription factor was amplified from Addgene_58437 (FRP880_PACT1(−1-520)-LexA-ER-haB112-TCYC1 was a gift from Joerg Stelling ...
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bioRxiv - Neuroscience 2019Quote: ... recombinant mouse E1 (Addgene plasmid # 32534), E2 (pGEX4T-1-UbcH5b) ...
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bioRxiv - Genomics 2021Quote: ... These cells were co-transfected with lentiviral packaging plasmids like VSVG (a gift from Bob Weinberg, Addgene #8454) and PAX2 (a gift from Didier Trono ...
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bioRxiv - Molecular Biology 2019Quote: ... Plasmid pAc-sgRNA-Cas9 was a gift from Ji-Long Liu (Addgene plasmid # 49330). The codons of LwaCas13a gene were optimized to Drosophila preference ...
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bioRxiv - Synthetic Biology 2019Quote: ... EP pol I and WT pol I (19)) were obtained from Addgene and recloned into a vector backbone carrying a spectinomycin resistance gene (SpecR ...
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bioRxiv - Microbiology 2020Quote: All virus-like particles were generated in HEK293T cells via calcium phosphate transfection using packaging plasmids psPAX2 (Addgene #12260) and pMD2.G (Addgene #12259) ...
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bioRxiv - Cell Biology 2023Quote: pENTR H2B mScarlet-I was created by PCR amplification of mScarlet-I from plasmid EB3- mScarlet-I (Addgene #98826, Dorus Gadella), using the primers FW 5’- ACCGGTGAATTCACCATGGTGAGCAAGGGCG-3’ and RV 5’-
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bioRxiv - Microbiology 2020Quote: ... Recombinant strains harbouring pTEC19 plasmid (Addgene, #30178) and producing the fluorescent protein E2-Crimson were grown in Middlebrook 7H9 broth supplemented with 0.2% glycerol (v/v ...
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bioRxiv - Biochemistry 2023Quote: ... and YFP tagged recombinant proteins (Addgene #173080), genes were inserted between N-terminal 6x His-tag followed by CFP/YFP tag and a TEV protease cleavage site of pNIC28-Bsa4 ...
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bioRxiv - Cell Biology 2021Quote: ... Lck-mScarlet-I and Lifeact-mScarlet-I were gifts from Dorus Gadella (Addgene plasmids #98821 ...
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bioRxiv - Cell Biology 2019Quote: The Polo-like binding domain (PBD) of PLK1 (amino acid 326 to amino acid 603) was amplified from the pTK24 plasmid (Addgene) and cloned into a pT7-His6-SUMO expression vector using NEB Gibson assembly (Gibson Assembly Master Mix ...
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bioRxiv - Immunology 2022Quote: ... lentivirus-like particles were made by transfecting HEK293T cells with the plasmids psPAX2 (gift from Didier Trono, Addgene plasmid # 12260), pMD2.G (gift from Didier Trono ...
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bioRxiv - Microbiology 2023Quote: ... SARS-CoV-2 virus-like particles were prepared as previously described (68) by co-transfecting plasmids for N (Addgene 177937); M and E (Addgene 177938) ...
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bioRxiv - Bioengineering 2023Quote: A DNA sequence encoding the CD47 Ig-like domain (Gln19 – Ser135) was cloned into the pCTCON2 yeast-surface display vector (Addgene) using the NheI and BamHI sites ...
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bioRxiv - Genetics 2023Quote: ... following the manufacturer’s protocol with AAVS1 targeting vector and predesigned transcription activator-like effector nucleases (hAAVS1 TALEN Left and Right were gifts from Su-Chun Zhang, Addgene plasmid # 52341 & 52342 ...
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bioRxiv - Developmental Biology 2024Quote: ... bovine growth hormon poly(A) signal (Addgene #132775) cloned into pUC18 vector (Addgene plasmid #50004 ...
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bioRxiv - Developmental Biology 2024Quote: ... bovine growth hormon poly(A) signal (Addgene #132775) cloned into pUC18 vector (Addgene plasmid #50004 ...
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bioRxiv - Developmental Biology 2024Quote: ... bovine growth hormon poly(A) signal (Addgene #132775) cloned into pUC18 vector (Addgene plasmid #50004 ...
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bioRxiv - Developmental Biology 2024Quote: ... bovine growth hormon poly(A) signal (Addgene #132775) cloned into pUC18 vector (Addgene plasmid #50004 ...
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bioRxiv - Developmental Biology 2024Quote: ... bovine growth hormone poly(A) signal (Addgene #132775) cloned into pUC18 vector (Addgene plasmid #50004 ...
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bioRxiv - Microbiology 2022Quote: ... melanogaster U6 promoter in the pAc-sgRNA-Cas9 (gifted by Ji-Long Liu, Addgene plasmid #49330) (81 ...
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bioRxiv - Neuroscience 2023Quote: ... For long-term silencing a cre dependent TelC AAV (AAV5-hSyn-FLEX-TeLC-P2A-dTomato, Addgene) was used and for long-term activation a cre dependent NaChBac (AAV-Syn-DIO-NaChBac-dTomato ...
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bioRxiv - Cell Biology 2022Quote: ... or mScarlet-I (Addgene #98839) and assembled into a custom Golden Gate expression vector (pGG) ...
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bioRxiv - Neuroscience 2023Quote: ... Lck-mScarlet-I (Addgene 98821), pCS2+-Vhh-CD8-mCherry (cloned from Gal4/LexA-Vhh-CD8-mCherry (Affolter lab ...
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bioRxiv - Neuroscience 2024Quote: ... 4xmts-mScarlet-I (Addgene, #98818), TurboID (Addgene ...
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bioRxiv - Microbiology 2022Quote: The SARS CoV-2 papain-like protease (PLpro) domain of Nsp3 was cloned from a doxycycline-inducible piggyBac transposon vector (PB-TAC-ERP2, Addgene# 80478) containing the synthesized full-length Nsp3 from the Wuhan-Hu-1 SARS CoV 2 strain (Alvarez and Yao ...