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Citations for Addgene :
1 - 50 of 1039 citations for Recombinant Human AKT1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: HIS-tagged HER2 (Addgene #16257) was expressed using the Expi293™ expression system (Thermo Fisher ...
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bioRxiv - Cell Biology 2020Quote: 6×His-tagged VHH-mCherry (Addgene #109421) was transformed into BL21DE3 E.coli cells ...
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bioRxiv - Cell Biology 2020Quote: ... and 6x-HIS-tagged-HOXB2 (Addgene 8522) were obtained from commercial vendors and cloned into pCMV6 vector and transfected into HEK cells ...
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bioRxiv - Biophysics 2020Quote: ... overnight dialysis and subsequent Sumo-tag cleavage by human sumo protease (His-tagged SenP1; Addgene #16356) 44 were performed in 50 mM Tris/HCl ...
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bioRxiv - Biochemistry 2023Quote: ... and YFP tagged recombinant proteins (Addgene #173080), genes were inserted between N-terminal 6x His-tag followed by CFP/YFP tag and a TEV protease cleavage site of pNIC28-Bsa4 ...
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bioRxiv - Immunology 2022Quote: ... Akt1 (Addgene #86631), Akt1T308A (Addgene #49189) ...
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bioRxiv - Cell Biology 2019Quote: ... His-tagged SEPT5 plasmid was constructed by PCR amplifying SEPT5 from pCMV-Myc-tagged SEPT5 (Addgene plasmid # 27272 ...
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bioRxiv - Cell Biology 2019Quote: His-tagged ubiquitin construct was obtained from Addgene (plasmid: #18712). siRNA#1 resistant RNF168-mCherry plasmid and RNF168 siRNAs were gift from Jiri Lucas (1) ...
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bioRxiv - Neuroscience 2020Quote: ... Human His-tagged SETD1A expression pET28-SETD1A-MHL plasmid and its control pET28-MHL were gifts from Cheryl Arrowsmith (Addgene plasmid # 32868 and #26096 ...
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bioRxiv - Cancer Biology 2019Quote: ... respectively (pHRIG-Akt1 (Addgene plasmid #53583) was a gift from Heng Zhao [66]) ...
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bioRxiv - Cell Biology 2020Quote: ... Histidine-tagged ubiquitin (pCI-His-hUbi, #31815) and Ha-tagged p62 (HA-p62, #28027) plasmids were purchased from Addgene. DNA constructs corresponding to the mature form of human UBTD1 were subcloned from pEGFP-N1 (Novagen ...
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bioRxiv - Biochemistry 2024Quote: Recombinant human GST-CK2α’ (Addgene #27084) and recombinant human GST-CK2α (Addgene #27083 ...
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bioRxiv - Cell Biology 2020Quote: Wild type AKT1 plasmid was purchased from Addgene (Addgene plasmid pcDNA3-myr-HA-AKT1 1036). Point mutation as in references (37 ...
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bioRxiv - Biochemistry 2024Quote: ... and recombinant human GST-CK2α (Addgene #27083) enzymes were produced in-house as previously described [30] ...
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bioRxiv - Cancer Biology 2020Quote: ... V5/His-tagged pLenti-puro-LacZ and pLenti-puro-ARID1A were obtained from Addgene.
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bioRxiv - Synthetic Biology 2022Quote: ... C-terminal His-tagged eGFP plasmid was from our lab stock (Addgene, No. 178422)32 ...
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bioRxiv - Cell Biology 2020Quote: Wild type AKT1 plasmid was purchased from Addgene (Addgene plasmid pcDNA3-myr-HA-AKT1 1036) ...
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bioRxiv - Cancer Biology 2022Quote: ... 901 pLNCX myr HA Akt1 (Addgene, Cat #9005) was used for transient overexpression of active AKT ...
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bioRxiv - Cell Biology 2020Quote: ... VSV-G-tagged human Lrp6 was obtained from Addgene (27282), amplified by PCR using primers containing Gateway sequences ...
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bioRxiv - Molecular Biology 2020Quote: ... His-tagged wild-type and D135S AlkB plasmids were obtained from Addgene (#79050 and #79051) and proteins were purified by a Ni-NTA column followed by cation exchange ...
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bioRxiv - Molecular Biology 2020Quote: ... strains of interest were transformed with a plasmid containing His-tagged SUMO (Smt3-Hisx7) (Addgene) under the control of a copper inducible promoter ...
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bioRxiv - Cancer Biology 2023Quote: Human AKT1_D274A was generated by overlapping PCR mutagenesis using wild-type AKT1 (a gift from Thomas Leonard, Addgene plasmid 8656133) and cloned into a pAceBac1 vector with N-terminal His10-StrepII-(tev ...
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bioRxiv - Immunology 2023Quote: ... pCDNA3-HA-Akt1-aa1-149 (Addgene plasmid # 73410, RRID:Addgene_73410), pCDNA3-HA-Akt1-aa1-408 (Addgene plasmid # 73412 ...
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bioRxiv - Immunology 2023Quote: ... pCDNA3-HA-Akt1-aa1-408 (Addgene plasmid # 73412, RRID:Addgene_73412) and pCDNA3-HA-Akt1-aa120-433 (Addgene plasmid # 73411 ...
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bioRxiv - Immunology 2023Quote: ... pCDNA3-HA-Akt1-aa1-408 (Addgene plasmid # 73412, RRID:Addgene_73412) and pCDNA3-HA-Akt1-aa120-433 (Addgene plasmid # 73411 ...
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bioRxiv - Immunology 2023Quote: ... pCDNA3-HA-Akt1-aa1-149 (Addgene plasmid # 73410, RRID:Addgene_73410), pCDNA3-HA-Akt1-aa1-408 (Addgene plasmid # 73412 ...
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bioRxiv - Synthetic Biology 2022Quote: A plasmid construct containing only the maturation protein and his-tagged coat protein dimer (Addgene # 179156) was transformed into Rosetta2™ (DE3 ...
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bioRxiv - Biochemistry 2022Quote: ... coli and subcloned in-line with his-tagged MBP construct (2CT-10 vector, Addgene plasmid #55209). Recombinant MBP-EndoH fusion was expressed in and purified from E ...
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bioRxiv - Developmental Biology 2019Quote: ... and HA-tagged human Snai1 (clone 31697) were purchased from Addgene. Full-length cDNA clones for human DDX3X (Accession ...
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bioRxiv - Biophysics 2023Quote: Histidine-tagged human RAD52 FL (RAD52 FL) expression vector (pET15b; Addgene) was transformed in E ...
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bioRxiv - Immunology 2023Quote: Human CD86 C-terminally tagged with enhanced GFP (pCD86-EGFP, Addgene) was sub-cloned into a modified version of the MSCV2.2 retroviral plasmid in which the IRES-GFP cassette was removed ...
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bioRxiv - Cell Biology 2023Quote: ... Plasmids encoding the human UBF gene tagged with EGFP and nucleolin gene tagged with EGFP were obtained from Addgene (plasmids # 26672 and #28176 ...
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bioRxiv - Microbiology 2021Quote: ... The recombinant plasmid containing human ACE2 gene (Addgene #1786) was transfected into HEK293T cells using Lipofectamine 2000 Transfection Reagent (Thermo Fisher ...
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bioRxiv - Neuroscience 2023Quote: Full-length recombinant human WT α-syn (Addgene #213498), α-syn 1-95 (Addgene #213499) ...
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bioRxiv - Cancer Biology 2020Quote: ... Myr-Akt1 and RFP-GGA1-NGAT were purchased from Addgene. LRP1-EGFP was a kind gift from S ...
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bioRxiv - Immunology 2023Quote: ... and pCDNA3-HA-Akt1-aa120-433 (Addgene plasmid # 73411, RRID:Addgene_73411). These plasmids were a gift from Jie Chen (University of Illinois at Urbana-Champaign ...
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bioRxiv - Immunology 2023Quote: ... and pCDNA3-HA-Akt1-aa120-433 (Addgene plasmid # 73411, RRID:Addgene_73411). These plasmids were a gift from Jie Chen (University of Illinois at Urbana-Champaign ...
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bioRxiv - Biophysics 2020Quote: ... Two plasmids containing full-length untagged Npl4 and C-terminal His-tagged Ufd1 were purchased from Addgene. Npl4 fragments were expressed in pET-28a vector with an N-terminal His-SUMO tag ...
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bioRxiv - Biochemistry 2022Quote: Codon optimized plasmids encoding the individual His-tagged PBRM1 bromodomain constructs were received from Nicola Burgess-Brown (Addgene plasmid numbers 38999 ...
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bioRxiv - Molecular Biology 2021Quote: pcDNA3.1-HA-AKT1 vector was purchased from Addgene (#9008, MA, USA); pEZ-M56-14-3-3γ-mCherry ...
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bioRxiv - Immunology 2023Quote: ... with the following plasmids: pCDNA3-HA-Akt1 (Addgene plasmid # 73408, RRID:Addgene_73408), pCDNA3-HA-Akt1-aa1-149 (Addgene plasmid # 73410 ...
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bioRxiv - Immunology 2023Quote: ... with the following plasmids: pCDNA3-HA-Akt1 (Addgene plasmid # 73408, RRID:Addgene_73408), pCDNA3-HA-Akt1-aa1-149 (Addgene plasmid # 73410 ...
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bioRxiv - Neuroscience 2022Quote: ... The V5-tagged human GLUT1 construct was a gift from Wolf Frommer (Addgene) 89 ...
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bioRxiv - Cancer Biology 2020Quote: ... FLAG-tagged human EZHIP was cloned into the pMT-puro vector (Addgene #17923). Transfections were performed with 2 μg plasmid DNA ...
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bioRxiv - Cell Biology 2023Quote: A vector expressing HA-tagged human E6AP was obtained from Addgene (Plasmid #8658), E6AP mutants were generated using site-directed mutagenesis (Agilent ...
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bioRxiv - Biochemistry 2022Quote: ... The recombinant human PRKACA cDNA was obtained from Addgene (Plasmid #23495) and cloned into between BamHI and KpnI in pcDNA5 vector (Invitrogen) ...
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bioRxiv - Immunology 2022Quote: ... An amino-terminal 3xFlag epitope tagged human GLUT3 was generated by PCR (Addgene #72877) (Supplementary Table 1) ...
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bioRxiv - Cancer Biology 2020Quote: ... Human DLK1 ectodomain expression vector was obtained from Addgene (DLK1-bio-His, RRID:Addgene_51876) (Sun et al. ...
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bioRxiv - Biochemistry 2022Quote: ... site-directed mutagenesis was conducted on pcDNA3-HA-Akt1-K179M (Addgene plasmid #73409) and pcDNA3-SGK1-V5-6xHis to change their original tags into FLAG tag ...
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bioRxiv - Biochemistry 2021Quote: ... The pcDNA3 Myr HA AKT1 plasmid was purchased from Addgene (ID 9008, RRID:Addgene_9008), which was originally established by Dr William Sellers (41) ...