Labshake search
Citations for Addgene :
1 - 50 of 178 citations for Pyruvate Kinase Microplate Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Physiology 2023Quote: FLAG-IKK2 kinase inactive K44M (IKK2DN, Addgene, #15466) was cloned into pLenti-CMV-Puro DEST vector (Addgene ...
-
bioRxiv - Biochemistry 2019Quote: ... sapiens Pyk2 kinase domain expression vector PTK2BA encoding H6-TEV-kinase [414-692] was a gift from Nicola Burgess-Brown (Addgene # 42401). Cloning vector pET-H6-SUMO-TEV-LIC (1S ...
-
bioRxiv - Molecular Biology 2022Quote: ... or pRK5-MYC-mTOR kinase-dead (Addgene plasmid #8482)) using the TransIT-X2 transfection reagent (Mirus ...
-
bioRxiv - Cancer Biology 2023Quote: A human kinase domain-focused gRNA library (Addgene 117725) [25] was used to assess CRISPR Cas9 screening as a tool in breast cancer PDTO to identify whether novel vulnerabilities could be detected for each PDTO ...
-
bioRxiv - Cancer Biology 2022Quote: ... and constitutively kinase-active (#64608) IKKα were purchased from Addgene. Briefly ...
-
bioRxiv - Biochemistry 2019Quote: ... with Protein kinase A (M. musculus PKA catalytic subunit alpha; Addgene 14921) expressed with an N-terminal His tag ...
-
bioRxiv - Cell Biology 2020Quote: ... A negative selection cassette with human thymidine kinase was retrieved from Addgene #21911 (41) ...
-
bioRxiv - Cell Biology 2020Quote: ... GST-Snf1 kinase domain (Snf1-KD) expression vector was purchased from Addgene (#52683). Those DNA constructs were transformed in Rosetta2 (Novagen ...
-
bioRxiv - Cell Biology 2022Quote: Full-length ERK3 wild type (WT) pDONR-223 construct purified from Human Kinase Library (Addgene) was used as a template to generate ERK3 K49A K50A kinase dead (KD ...
-
bioRxiv - Cell Biology 2023Quote: ... we expressed the ULK1 kinase from a pCAG backbone encoding MBP-TSF-TEV-ULK1 (RRID:Addgene_171416). The transfection and expression procedure was similar to what is described above for NAP1 ...
-
bioRxiv - Cell Biology 2024Quote: ... DNA fragments containing MAP3K1 (MEKK1) or kinase-dead MAP3K1 were excised from pCDNA-MEKK1 plasmids (Addgene #12181 and #12180 ...
-
bioRxiv - Neuroscience 2021Quote: ... expressing the genetically encoded calcium indicator GCaMP6f under control of the calcium/calmodulin-dependent protein kinase (CaMKII) promoter (pENN.AAV5.CAMKII.GCaMP6f.WPRE.SV40, Addgene, Watertown, MA) to drive expression preferentially in principal neurons ...
-
bioRxiv - Genetics 2023Quote: ... fused to the herpes simplex virus thymidine kinase-encoding gene (HSV-TK) from the pFA6a-HyTkAX vector (Addgene plasmid # 73898; http://n2t.net/addgene:73898; RRID:Addgene_73898) [80] ...
-
bioRxiv - Neuroscience 2023Quote: ... expressing the genetically encoded calcium indicator GCaMP6f under control of the calcium/calmodulin-dependent protein kinase (CaMKII) promoter (pENN.AAV5.CAMKII.GCaMP6f.WPRE.SV40, Addgene, Watertown, MA). Virus (0.5 µl ...
-
bioRxiv - Microbiology 2024Quote: ... Wild-type Myc-MEKK3 (in pcDNA3.1 vector) was generated from a kinase-dead mutant (K391A, Addgene plasmid # 44157)(55 ...
-
bioRxiv - Biochemistry 2019Quote: ... and a mammalian expression vector pcDNA3 encoding hemagglutinin tagged constitutively active glycogen synthase kinase-3β (GSK-3β) (S9A) (pcDNA3-GSK3β, # 14754, RRID: Addgene_14754). The EGFP in pRK5 vector was first replaced by iRFP to construct an iRFP tagged WT tau by golden gate assembly (pRK5-iRFP-tau ...
-
bioRxiv - Cell Biology 2021Quote: ... CHO cell lines were transfected with a reporter construct containing three copies of the PPRE placed upstream of the thymidine kinase promoter-firefly luciferase fusion gene (PPRE X3-TK-luc, Addgene). In addition ...
-
bioRxiv - Developmental Biology 2019Quote: Two gRNAs targeting loci near the start codon of the Rho kinase gene were cloned into pCFD3 vector (Addgene 49410) following the protocol from (Port et al. ...
-
bioRxiv - Cancer Biology 2020Quote: ... Select MOC1 clones that show lack of protein expression of RIP3 were transduced with inducible lentiviral vectors encoding WT (Plasmid #73701) or kinase-dead mutant (D143N) RIP3 (Plasmid #73703) purchased from Addgene (These plasmids were gifted by Dr ...
-
bioRxiv - Genetics 2023Quote: ... we first deleted the ORF with a cassette containing the hygromycin resistance marker (hphRMX) fused to the herpes simplex virus thymidine kinase-encoding gene (HSV-TK) from the pFA6a-HyTkAX vector (Addgene plasmid # 73898 ...
-
bioRxiv - Cell Biology 2020Quote: ... The recombinant WNK1 kinase domain was eluted via addition of 50 nM bdSENDP1 protease (Frey et al., 2014; Addgene ID 104962) in wash buffer 4.
-
bioRxiv - Neuroscience 2021Quote: ... targeting LRRK2 sequence near GTP binding and ATP kinase binding domains were synthesized and cloned into pSpCas9(BB)-2A-GFP plasmid (Addgene #48138). Plasmids were transfected into A18945 iPSC line using Lipofectamine-Stem transfection reagent (ThermoFisher #STEM00001 ...
-
bioRxiv - Cancer Biology 2021Quote: ... Tag5Amyc-GSK3β S9A (constitutively active GSK3β, plasmid #16261), and Tag5Amyc-GSK3β K85A (Kinase dead GSK3β, plasmid #16262) were from Addgene (Watertown, MA). Cyclin D3 cDNA was cloned out of Rc/CMV cyclin D3 (CMV promoter ...
-
bioRxiv - Cell Biology 2020Quote: ... Lentiviral expression vector encoding the p38 MAPK Kinase Translocation Reporter (KTR) was a kind gift from Markus Covert (Addgene plasmid no. 59155). ON-TARGETplus SMARTpool siRNAs for the genes of interest as well non-targeting negative control siRNAs were obtained from Dharmacon (Lafayette ...
-
bioRxiv - Cell Biology 2022Quote: ... annealed oligos were phosphorylated in vitro with T4 polynucleotide kinase (#M0201S) from New England Biolabs (Ipswich, MA, USA) and ligated into pDR274 vector (#42250) from Addgene (Watertown, MA, USA), which was previously linearized with BsaI (#R0535 ...
-
bioRxiv - Biophysics 2022Quote: The plasmid encoding GPCR kinase subtype 2 used for the receptor phosphorylation in insect cells (GRK2-CAAX) was a gift from Robert Lefkowitz (Addgene plasmid #166224 49). Full-length arrestin21-418 (C150L ...
-
bioRxiv - Genomics 2023Quote: To generate a functional mouse sgRNA pooled library of the pAP210 and pAP215 plasmids, we transferred the sgRNA sequences from the mCRISPRi-v2 M1 (Kinases, Phosphatases, and Drug Targets) gRNA pooled library (Addgene pooled library #83989)10 ...
-
bioRxiv - Neuroscience 2023Quote: ... 5% FBS,0.01% Sodium Pyruvate) were transfected according to manufacturer instructions using Lipofectamine 2000 with plasmids carrying HIV-1 Gag/Pol (pMDLg/pRRE Addgene: 12251), HIV-1 Rev (pRSV-Rev Addgene ...
-
bioRxiv - Bioengineering 2023Quote: Full length plasmid of PI3KCA (phosphatidylinositol-4,5-biphosphate 3-kinase catalytic subunit alpha, NM_006218.4) was purchased from Addgene (Plasmid ID: 81736, Hahn and Root Lab). The coding sequence of PI3KCA was cloned into Lenti-PCDH-EF1-mNeonGreen-MCS-T2A-puromycin plasmid (a kind gift from Fırat-Karalar Lab ...
-
bioRxiv - Cell Biology 2020Quote: ... The FRET-based lactate biosensor [Laconic/pcDNA3.1(-)] and pyruvate biosensor [Pyronic/pcDNA3.1(-)] were gifts from Luis Felipe Barros (Addgene plasmid #44238, and #51308) [43,44] ...
-
bioRxiv - Cancer Biology 2022Quote: ... or EJ5GFP for NHEJ assay (Addgene, Plasmid 44026) were generated as previously described (20) ...
-
bioRxiv - Cancer Biology 2022Quote: We employed the Traffic Light Reporter assay (Addgene #31482) to look at HR/altNEHJ efficiency ...
-
bioRxiv - Genetics 2021Quote: The Downstream assay vector was based on a pSMART backbone (Addgene plasmid # 49157 ...
-
bioRxiv - Microbiology 2021Quote: Plasmids for luciferase assays were purchased from Addgene (ID: 105494 – 105497) and plasmids for testing interaction in vegetative cells were constructed following previously established protocols (39 ...
-
bioRxiv - Biochemistry 2022Quote: ... PRESTO Tango assay constructs were acquired from Addgene (Cat. no. 1000000068) while the Tango assay constructs were generated based on previously described framework (Barnea et al. ...
-
bioRxiv - Molecular Biology 2023Quote: The following plasmids were purchased from Addgene and used for the RaPID-MS assay: BoxB-plasmid (Addgene #107253); BoxB-EDEN15 plasmid (#107252) ...
-
bioRxiv - Synthetic Biology 2023Quote: ... The YTK kit (Addgene; Kit #1000000061) was used as a source for all relevant non-coding DNA sequences — including promoters and terminators ...
-
bioRxiv - Biochemistry 2022Quote: Plasmid cleavage assays were performed using the eGFP-hAgo2 plasmid (Addgene #21981) as target DNA ...
-
bioRxiv - Synthetic Biology 2022Quote: ... MoClo Plant Parts Kit (Addgene Kit #1000000047), GreenGate Cloning System (Addgene Kit #1000000036 ...
-
bioRxiv - Cancer Biology 2022Quote: HN30 and HN31 cell lines with pDRGFP for HR assay (Addgene, Plasmid #26475) or EJ5GFP for NHEJ assay (Addgene ...
-
bioRxiv - Molecular Biology 2023Quote: ... The R6K plasmid for mate-out transposition assays was obtained from Addgene (#64968) [66] ...
-
bioRxiv - Neuroscience 2023Quote: The generation of plasmids for this assay was previously described and obtained from Addgene [7] ...
-
bioRxiv - Neuroscience 2023Quote: The plasmid RHCglo used for minigene assay was a gift from Thomas Cooper (Addgene plasmid #80169 ...
-
bioRxiv - Microbiology 2020Quote: ... Cas9 activity was checked using the XPR_047 assay (a gift from David Root, Addgene 107145) and was 79.5% and >83.4% ...
-
bioRxiv - Neuroscience 2023Quote: ... Chimeric G proteins for the Gsx assay 44 were obtained from Addgene (Watertown, MA, USA).
-
bioRxiv - Systems Biology 2022Quote: ... and lambda phosphatase were purchased from Addgene (Addgene Kit #1000000094)7.
-
bioRxiv - Microbiology 2021Quote: ... WT Cas9 activity was checked using the XPR_047 assay (a gift from David Root, Addgene 107145) and was always >80-90% ...
-
bioRxiv - Cancer Biology 2021Quote: ... Reporter assays to assess RUNX1 transcriptional activity were done using the pMCSF-R-luc plasmid (Addgene plasmid #12420 ...
-
bioRxiv - Cancer Biology 2022Quote: The mismatch repair assay was performed as described previously57 using plasmids pGEM5Z (+)-EGFP (Addgene, cat #65206) and p189 (Addgene ...
-
bioRxiv - Cell Biology 2022Quote: Luciferase assays were performed in HEK293T cells using the YAP/TAZ reporter 8xGTIIC-lux (Addgene, 34615). Renilla-Luc (0.05 µg/well ...