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Citations for Addgene :
1 - 50 of 526 citations for Protein Digestion kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... by digestion-ligation reaction in plCH47751 (Addgene #48002) and plCH47761 (Addgene #48003 ...
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bioRxiv - Genetics 2022Quote: ... After digestion of pKHR8 (Addgene#74625; Hoshijima et al. 2016) using BamHI and SalI in MCSs ...
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bioRxiv - Developmental Biology 2022Quote: The larger fragment from restriction digestion of pAAVS1-PDi-CRISPRn (Addgene 73500) using AgeI and PacI was ligated to annealed DNA from two oligoes ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: The GLP-1R-SmBiT plasmid was generated following digestion of GLP-1R-Tango (Addgene plasmid # 66295 ...
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bioRxiv - Neuroscience 2021Quote: ... and subsequently combined into one vector by SacII digestion and ligation (Addgene, plasmid #122563). The Cre sequence was amplified using pCR8GW-Cre-pA-FRT-kan-FRT as template DNA (Suster et al. ...
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bioRxiv - Physiology 2020Quote: ... Human FL-Dhh sequence was obtained after EcoRI digestion of pBS hSHH (Addgene ID13996) and then cloned at the EcoRI site of pCDNA3.1 myc His to generate the pcDNA3-FL-Shh plasmid ...
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bioRxiv - Cell Biology 2020Quote: ... BioID2-pcDNA5 FRT/TO was created by restriction digestion of BioID2 from myc-BioID2-MCS (74223, Addgene) using NheI and XhoI ...
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bioRxiv - Biochemistry 2023Quote: ... These mutations were corrected here by ligating the NotI/SacI digestion fragment from pCMV-BE4max (Addgene #112093) into the digested AID’-BE4max (Addgene #174696 ...
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bioRxiv - Neuroscience 2021Quote: ... The Gbait-hsp70 fragment was subcloned into the Cre vector by SacII digestion and ligation (Addgene, plasmid #122562).
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bioRxiv - Genetics 2021Quote: ... into the pActin5c-attB-RFP vector isolated by digestion of pActin5c-Cas9 (Addgene# 62209 (Port et al., 2014)) with EcoRI/KpnI ...
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bioRxiv - Genomics 2024Quote: The TRE3G-dCas9-VPR fragment was obtained by digestion of the PB-TRE-dCas9-VPR vector (Addgene #63800) with PmeI and PspXI ...
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bioRxiv - Neuroscience 2021Quote: ... The EGFP-CCR5TC fragment was obtained by a BamHI and XhoI digestion from pCAG-GPHN.FingR-EGFP-CCR5TC (Addgene #46296)17 ...
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bioRxiv - Cell Biology 2020Quote: ... The construct pT2-CAG-GFP-E-Syt1 was generated by restriction digestion of the EGFP-E-Syt1 (Addgene #66830) and the pT2-CAG-fGFP plasmids with AgeI and MluI ...
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bioRxiv - Neuroscience 2020Quote: ... The constructs were subcloned using restriction digestion into the pHL-sec vector containing a C-terminal 6xHis-tag (Addgene # 99845). Restriction sites for subcloning were introduced by PCR at the 5’ and 3’ ends of scFv-Clasp heavy and light chains (light chain ...
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bioRxiv - Cell Biology 2021Quote: ... followed by digestion of the PCR product with EcoRI and SacII and ligation in between Smoothened and mCherry in mCherry-Smo (Addgene plasmid 55134 ...
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bioRxiv - Genetics 2022Quote: ... with 5’ KpnI and 3’ EcoRI sites (primers LC127 and 128) into the corresponding restriction digestion sites of pLP9 (Addgene plasmid #1497 ...
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bioRxiv - Synthetic Biology 2020Quote: ... we began by mutating the two Esp3I digestion sites in the LentiCRISPR v2 plasmid (gift from Feng Zhang, Addgene #52961)(47 ...
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bioRxiv - Cancer Biology 2022Quote: ... a central region containing a triple FLAG epitope and P2A-neomycin resistance cassette acquired via restriction endonuclease digestion of pFETCH_Donor (Addgene: 63934); and 3 ...
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bioRxiv - Bioengineering 2022Quote: ... After digestion with EcoRI and AgeI the PCR products were ligated into the pLVX-TetOne-Puro vector (Addgene plasmid #124797).
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bioRxiv - Immunology 2023Quote: DNA inserts containing the coding DNA sequence (CDS) for each wild type gene were generated by restriction digestion previous amplification from plasmids obtained from Addgene (hMX1 ...
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bioRxiv - Cell Biology 2022Quote: A His-tagged mouse Capping Protein (F-actin-capping protein subunit alpha-1 and F-actin-capping protein subunit beta) plasmid was obtained from Addgene as described above (Plasmid #89950) ...
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bioRxiv - Cell Biology 2022Quote: ... Tau fragments were subcloned into pcDNA3.1 by restriction digestion and further into pCW57.1- MAT2A all-in-one tet-off lentiviral backbone (a gift from David Sabatini (Addgene plasmid # 100521))71 by Gibson assembly ...
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bioRxiv - Cell Biology 2023Quote: ... Cloning was performed using the single-step digestion-ligation protocol from the Zhang lab (available on the Zhang lab Addgene page). To validate each guide ...
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bioRxiv - Plant Biology 2023Quote: ... These Level 0 promoter parts were used in in one-step digestion-ligation reactions with the Level 1 Loop pCk1 (Addgene #136695) backbones together with parts containing LucN (pEPYC0CM0133 ...
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bioRxiv - Cancer Biology 2023Quote: ... the lentiviral plasmid pLenti-fLuc-P2A-tCD19 was generated by excising with AjiI and MluI digestion the Neomycin resistance in the plasmid Lenti-luciferase-P2A-Neo (Addgene, #105621) and replacing it by GIBSON assembly with the tCD19 sequence amplified by PCR from the vector pcDNA3.1+CD19-C-DYK (Genescript ...
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bioRxiv - Developmental Biology 2022Quote: ... Samples were incubated with Protein A/G-MNase fusion protein (Addgene, Cat #123461) at 700 ng/mL for 1 hour at 4°C ...
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High-performance GPCR optogenetics based on molecular properties of animal opsins, MosOpn3 and LamPPbioRxiv - Biochemistry 2022Quote: ... The vector backbone containing SL1 and GFP was obtained by digestion of the plasmid [pEM1 = flp-21::LoxPStopLoxP::npr-1 SL2 GFP] (Addgene plasmid # 24033)65 with NotI and KpnI ...
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bioRxiv - Evolutionary Biology 2020Quote: ... the fl-STOP-fl cassette was excised from the pHB4 vector (a gift from Dr Jason Chin) by restriction digestion with EcoRI and AscI and ligated into the pT2-CAG-fGFP (Addgene plasmid #108714) opened with the same restriction enzymes ...
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bioRxiv - Molecular Biology 2021Quote: ... Cloning into sgRNA plasmid vectors was performed using reverse complement oligos and the single-step digestion-ligation Zhang lab protocol (Broad Institute) into the pX459 background (Ran et al., 2013; Addgene plasmid #62988).
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bioRxiv - Microbiology 2022Quote: ... synthetic oligonucleotides containing sgRNAs (5’ sgRNA 5’- CACCGTCTAGGGAAAGTCATTCAG-3’ and 5’- AAACCTGAATGACTTTCCCTAGAC-3’ and 3’ sgRNA 2 5’- CACCGCAGTAATCTAATCTTTAAG-3’ and 5’-AAACCTTAAAGATTAGATTACTGC-3’ targeting the first coding exon of ACE2 were generated with overhangs for BbsI digestion and subsequent cloning into pX458 (Addgene, Cat. 48138) as described previously ...
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bioRxiv - Immunology 2021Quote: ... green fluorescence protein (GFP) or an enhanced yellow fluorescent protein (EYFP) sequence (pcDNA1.3-, Addgene) using standard cloning methods ...
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bioRxiv - Genetics 2019Quote: ... mCherry fluorescent protein marker (Addgene), and ampicillin resistance gene ...
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Hippocampal efferents to retrosplenial cortex and lateral septum are required for memory acquisitionbioRxiv - Neuroscience 2020Quote: ... enhanced yellow fluorescent protein (eYFP) or enhanced green fluorescent protein (eGFP): AAV1.CaMKIIa.eNpHR3.0.eYFP.WPRE.hGH (Addgene, #26971), AAV1.CaMKII.eYFP ...
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bioRxiv - Synthetic Biology 2022Quote: A plasmid construct containing only the maturation protein and his-tagged coat protein dimer (Addgene # 179156) was transformed into Rosetta2™ (DE3 ...
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bioRxiv - Genomics 2019Quote: ... we cloned effector proteins (PguCas13b: Addgene 103861 ...
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bioRxiv - Genetics 2019Quote: ... Cas9 protein and mRNA (44758; Addgene) were co-microinjected into zygotes [F1 hybrids between strains FVB/NJ and B6(Cg)-Tyrc-2J/J] using the reagent concentrations listed in Table S1 ...
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bioRxiv - Bioengineering 2021Quote: ... the Spike protein (Addgene plasmid # 145032) was cloned downstream of the TRE3G promoter ...
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bioRxiv - Bioengineering 2021Quote: ... We acquired psPAX2 encoding lentiviral packaging proteins and PMD2.G encoding a lentiviral envelop protein from Addgene (plasmid no ...
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bioRxiv - Molecular Biology 2022Quote: Plasmids pLVX-EF1alpha-SARS-CoV-2-proteins-2xStrep-IRES-Puro proteins are a gift from Nevan Krogan (Addgene)(Gordon ...
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bioRxiv - Genomics 2019Quote: ... we cloned the effector proteins (PguCas13b: Addgene 103861 ...
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bioRxiv - Molecular Biology 2020Quote: ... envelope protein plasmid (pMD2.G, Addgene #12259), REV-expressing plasmid (pRSV-Rev ...
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bioRxiv - Cell Biology 2021Quote: ... and envelope encoding protein (VSVG; Addgene # 8454). Lentiviral particles were collected after 48 hrs of transfection and were used for transducing target cell lines.
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bioRxiv - Cell Biology 2020Quote: ... or a fluorescent protein (pCDNA3-GFP; Addgene plasmid #74165 or mCherry2-N1 ...
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bioRxiv - Biochemistry 2023Quote: ... and YFP tagged recombinant proteins (Addgene #173080), genes were inserted between N-terminal 6x His-tag followed by CFP/YFP tag and a TEV protease cleavage site of pNIC28-Bsa4 ...
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bioRxiv - Biochemistry 2024Quote: ... envelope protein-pCMV-VSV-G (Addgene #8454), packaging plasmid - pCMV-dR8.2 dvpr (Addgene #8455 ...
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bioRxiv - Immunology 2021Quote: The SARS-CoV-2 pseudoviral particles expressing COVID-19 spike protein pGBW m4137384: S protein was purchased from Addgene (149543) and the virus particles were produced as describe previously (Hoffmann et al. ...
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bioRxiv - Cell Biology 2021Quote: ... Plasmid encoding for mitochondria specific protein/ autophagosome specific protein is transfected in HEK293T cells along with packaging vector (pDR8.2; Addgene #8455) and envelope encoding protein (VSVG ...
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bioRxiv - Cell Biology 2023Quote: ... Ras GTPase-activating protein-binding protein 1 (G3BP1) phage UbiC G3BP1-GFP-GFP was a gift from Jeffrey Chao (Addgene plasmid # 119950 ...
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bioRxiv - Systems Biology 2021Quote: ... and envelope protein pCMV-VSV-G (Addgene #8454) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2021Quote: ... and pBAD expressing fluorescent protein variants (Addgene, UK). Primers used in the assembly of the vectors are listed in SI Table 1.