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Citations for Addgene :
1 - 50 of 565 citations for Plant DNA Purification kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2022Quote: ... MoClo Plant Parts Kit (Addgene Kit #1000000047), GreenGate Cloning System (Addgene Kit #1000000036 ...
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bioRxiv - Plant Biology 2020Quote: ... GFP tagged OsHIPP19 and OsHIPP20 were generated by Golden Gate methods (Engler et al. 2008) using MoClo Plant Parts Kit and MoClo Plant Tool Kit (Addgene, UK). OsHIPP19 and OsHIPP20 were cloned into pICH47732 with a GFP tag at the N-terminus ...
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bioRxiv - Plant Biology 2022Quote: ... a modular cloning system was employed using MoClo Tool Kit and MoClo Plant Parts kit (Addgene, Supplemental Table S3) (Weber et al. ...
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bioRxiv - Plant Biology 2020Quote: ... The following binary plasmids were assembled by Golden Gate cloning using the MoClo Tool Kit for plants (Addgene kit #1000000044) (Weber et al. ...
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bioRxiv - Plant Biology 2024Quote: ... Plasmid construction was performed by modular cloning using the MoClo Tool Kit and the MoClo Plant Parts Kit (Addgene, 28–30). A detailed MoClo protocol and the list of vectors used can be found in 18.
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bioRxiv - Bioengineering 2022Quote: ... This plasmid was then used as a template for Golden Gate-based cloning (MoClo Plant Kit, Addgene) (47) ...
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bioRxiv - Plant Biology 2022Quote: ... p35S:MPTMV-YFP + ERmCherry and p35S:MPToBRFV-YFP + ER-mCherry were assembled by Golden Gate cloning using the MoClo tool kit for plants (Addgene) (Weber et al. ...
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bioRxiv - Biochemistry 2020Quote: ... soluble histag purification (Addgene: 139109)
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bioRxiv - Biochemistry 2020Quote: ... insoluble histag purification (Addgene: 98670)
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bioRxiv - Biochemistry 2020Quote: ... insoluble histag purification (Addgene: 98657)
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bioRxiv - Biochemistry 2020Quote: ... soluble histag purification (Addgene: 98651)
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bioRxiv - Biochemistry 2020Quote: ... soluble histag purification (Addgene: 104480)
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bioRxiv - Biochemistry 2020Quote: ... insoluble anion purification (Addgene: 98656)
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bioRxiv - Biochemistry 2020Quote: ... insoluble His-tag purification (Addgene ID ...
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bioRxiv - Biochemistry 2020Quote: ... soluble His-tag purification (Addgene ID ...
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bioRxiv - Biochemistry 2020Quote: ... His-tag purification (Addgene ID: 139113)
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bioRxiv - Synthetic Biology 2023Quote: ... We used DNA parts provided with the EMMA cloning kit (Addgene kit # 1000000119) and generated DNA fragments by PCR using Platinum™ SuperFi II Green PCR Master Mix (ThermoFisher ...
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bioRxiv - Plant Biology 2022Quote: ... were cloned into the plant expression vector (pMDC32-Ubi1; Addgene, USA), generating the final CRISPRi vector (pCTN ...
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bioRxiv - Biochemistry 2020Quote: ... soluble His-tag purification (Addgene ID: 139112)
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bioRxiv - Biochemistry 2020Quote: ... insoluble His-tag purification (Addgene ID: 139116)
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bioRxiv - Biochemistry 2020Quote: ... soluble His-tag purification (Addgene ID: 139117)
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bioRxiv - Synthetic Biology 2021Quote: The plant expression vector was generated using the plasmid pICH86966 (Addgene#48075) as the backbone ...
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bioRxiv - Plant Biology 2020Quote: ... Basic DNA parts were selected from GoldenBraid 2.0 kit from Diego Orzaez (Addgene kit # 1000000076) or MoClo Toolkit ...
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bioRxiv - Biochemistry 2020Quote: ... His-tag purification (Addgene ID: 139124, 139125 respectively)
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bioRxiv - Biochemistry 2020Quote: ... insoluble His-tag purification (Addgene ID: 139126, 139127, 139128)
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bioRxiv - Biochemistry 2020Quote: ... soluble His-tag purification (Addgene ID: 139114, 139115 respectively)
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bioRxiv - Plant Biology 2021Quote: ... GUS and AtuOCS sequences were obtained from the MoClo Plant Parts toolkit supplied by Addgene (www.addgene.org) (Engler et al. ...
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bioRxiv - Synthetic Biology 2022Quote: ... All three pS381 plasmids used in purifications are available from Addgene under the IDs 186733-186735.
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bioRxiv - Bioengineering 2021Quote: ... The sgRNA expression was driven by bacterial pJ23119 (synthetically cloned) or plant AtU6 promoter (pICSL01009, Addgene #46968). The nCas9(D10A ...
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bioRxiv - Plant Biology 2021Quote: ... We designed a mutant allele of a plant codon optimized Cas9 with a potato intron (Addgene: 117515) with D10A (nickase mutant ...
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bioRxiv - Synthetic Biology 2019Quote: ... synthetic gene fragments from Integrated DNA Technologies (IDT) and the EcoFlex kit (47) from Addgene.
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bioRxiv - Synthetic Biology 2020Quote: ... This reporter cassette was assembled with a plant selectable marker cassette conferring resistance to kanamycin (pEPYC1CB0308, Addgene T.B.C.). Synthetic and control promoters were additionally fused to either a transcription activator like effector (TALE ...
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bioRxiv - Cell Biology 2020Quote: FLOE1’s coding sequence was transferred from G18395 (see Plant vector construction section) into pDEST-HisMBP (Addgene plasmid # 11085) (32 ...
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bioRxiv - Plant Biology 2023Quote: ... Both constructs were subcloned into the plant destination vector pMDC32-HPB using LR clonaseTM (obtained from Addgene, https://www.addgene.org), harbouring a 2X CaMV 35S promoter upstream of the gateway recombination sites to generate 35S:RAPTOR1B-6xMyc ...
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bioRxiv - Molecular Biology 2023Quote: The plant sgRNA vectors (rice U3 promoter drives sgRNA) were constructed as reported previously using the pOsU3 backbone (Addgene#170132)60 ...
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bioRxiv - Plant Biology 2023Quote: ... L0 parts were synthesised either by GENEWIZ or Twist Bioscience following the standard syntax for plant synthetic biology with PROM5 or PROM and 5UTR overhangs and cloned into the plasmid pUAP1 (Addgene #63674) (Patron et al. ...
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bioRxiv - Plant Biology 2021Quote: ... pICH47811-pTCSn::nls:tGFP (position 2) was cloned together with pICH47802-pIPT3::nls:tdTOMATO::t35S (position 1) in the final plant expression vector pAGM4673 (Addgene, catalog number: 48014).
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bioRxiv - Plant Biology 2021Quote: ... was cloned together with pICH47802-p35S::ER:tdTOM::tNOS selection marker (position 1) in the final plant expression vector pAGM4673 (Addgene, catalog number: 48014). For simultaneous live imaging ...
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bioRxiv - Plant Biology 2020Quote: CaMV-35s promoter was amplified from pCambia2300 plasmid using the primer pair ApaI-EcoRI-35s-F and NcoI-35s-R and cloned in the ApaI/NcoI sites of pYPQ152 plasmid harboring the plant codon optimized deactivated Cas9 (Pco-dCas9) coding sequence (Addgene Plasmid #69303) to generate the pYPQ152_35s plasmid ...
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bioRxiv - Biochemistry 2020Quote: MBP-hnRNPA2 LC, soluble His-tag purification as described (Ryan et al., 2018) (Addgene ID: 98661)
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bioRxiv - Biochemistry 2020Quote: hnRNPA2 LC (190-341), insoluble His-tag purification as described (Ryan et al., 2018) (Addgene ID: 98657)
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bioRxiv - Cell Biology 2021Quote: ... lentivector DNA plasmids (Addgene plasmids # 27150 ...
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bioRxiv - Biophysics 2022Quote: ... GST-mCherry-FYVE (https://www.protocols.io/view/expression-and-purification-protocol-of-gst-mch-fy-b8k5ruy6) and His-TEV-ATG3 (Addgene_169079 ...
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bioRxiv - Biochemistry 2023Quote: ... This method is also applicable to the expression and purification of mCylLS (plasmid available at Addgene ID #208760).
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bioRxiv - Cancer Biology 2022Quote: ... 2μg of DNA (pX330 [Addgene # 42230] - sgFBXW7 or sgAPC ...
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bioRxiv - Cell Biology 2023Quote: The following DNA constructs (Addgene) were used in this study ...
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bioRxiv - Biochemistry 2020Quote: FUS LC and FUS LC 12E, soluble His-tag purifications as described (Monahan et al., 2017) (Addgene ID: 98653, 98654)
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bioRxiv - Neuroscience 2020Quote: ... mRuby2 DNA was purchased from Addgene (mRuby2-C1 ...
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bioRxiv - Bioengineering 2022Quote: ... Plasmid DNA (pm-mCherry-N1, Addgene) was labelled with Label It DNA kit (Mirus) ...
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bioRxiv - Cell Biology 2023Quote: A PIP-FUCCI coded DNA fragment was PCR amplified with template DNA (pENTR-PIP-FUCCI; Addgene; plasmid #118621), which was a gift from Jean Cook ...