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Citations for Addgene :
1 - 50 of 55 citations for PE2 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: pCMV-PE2 (Addgene plasmid #132775 ...
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bioRxiv - Genetics 2020Quote: PE2 coding sequence was PCR amplified from pCMV-PE2 (Addgene # 132775) and gel purified ...
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bioRxiv - Genetics 2020Quote: PE2 coding sequence was PCR amplified from pCMV-PE2 (Addgene # 132775). pEntr backbone was PCR amplified from pEntr_D-TOPO (Invitrogen K240020) ...
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bioRxiv - Biochemistry 2023Quote: ... The pCMV-PE2 plasmid expressing PE2 Cas9-RT fusion protein (Addgene plasmid #132775 ...
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bioRxiv - Bioengineering 2021Quote: ... the sequence encoding the PE2 N terminus (PE2-N term) (Addgene #132775) and a splicing donor (SD ...
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bioRxiv - Bioengineering 2021Quote: ... the sequence encoding the PE2 C terminus (PE2-C term) (Addgene #132775), and the WPRE sequence (Addgene #52962 ...
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bioRxiv - Genomics 2020Quote: ... pCMV-PE2 (Addgene #132775), pU6-pegRNA-GG-acceptor (Addgene #132777) ...
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bioRxiv - Genetics 2021Quote: Plasmids encoding PE2 (Addgene plasmid #13277519 ...
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bioRxiv - Molecular Biology 2021Quote: ... pCMV-PE2 (Addgene #132775) and pU6-pegRNA-GG-acceptor (Addgene #132777 ...
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bioRxiv - Neuroscience 2023Quote: ... The open reading frame of PE2 was extracted from pCMV-PE2 (Addgene plasmid #132775) by PCR with primers (prRR842 and prRR849) ...
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bioRxiv - Genetics 2020Quote: pUAS-PE2-attB (Addgene #149550) and pAct-PE2-HygroR (Addgene #149552 ...
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bioRxiv - Molecular Biology 2021Quote: ... PE2 sequence including the backbone corresponds to the previously published CMV-PE2 construct (Anzalone) (Addgene #132775). To generate PEn ...
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bioRxiv - Molecular Biology 2021Quote: pCMV-PE2-P2A-PuroR was generated by replacing eGFP from pCMV-PE2-P2A-GFP (Addgene 132776) with PuroR ...
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bioRxiv - Bioengineering 2021Quote: ... the PE2-encoding sequence (Addgene #132775) and the WPRE sequence (Addgene #52962 ...
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bioRxiv - Genetics 2020Quote: ... and pAct-PE2-HygroR (Addgene #149552) were generated by Gateway reactions between pEntr_PE2 and pWalium10-roe (Perkins et al ...
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bioRxiv - Bioengineering 2023Quote: ... vector pCMV-PE2 (Addgene plasmid # 132775), vector pCMV-BE3 (Addgene plasmids # 73021 ...
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bioRxiv - Molecular Biology 2022Quote: pLV-EF1a-PE2-P2A-GFP was generated by cloning the original pCMV-PE2-P2A-GFP insert (Addgene #132776) into an EF-1alpha driven lentiviral backbone by Signagen Inc (from here on called pLV-PE2) ...
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bioRxiv - Bioengineering 2021Quote: ... Fragments of PE2-encoding sequence (Addgene #132775) and NG-Cas9-encoding sequence (Addgene #124163 ...
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bioRxiv - Bioengineering 2020Quote: ... 1.5 ug pCMV-PE2 plasmid (Addgene #132775), 500 ng pegRNA plasmid (cloned into Addgene #132777) ...
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bioRxiv - Bioengineering 2021Quote: ... 375 ng of PE2 plasmid (Addgene #132775) and 125 ng of pegRNA or paired-pegRNA plasmid were mixed and prepared with transfection reagent (Lipofectamine 3000 ...
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bioRxiv - Genetics 2021Quote: ... and pCMV-PE2 (Addgene plasmid no. 132775) were gifts from David Liu ...
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bioRxiv - Bioengineering 2023Quote: PE2 plasmid was obtained from Addgene (#132775). The scFv sequence was derived from plasmid (Addgene #60904 ...
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bioRxiv - Molecular Biology 2023Quote: ... pCMV-PE2-tagRFP-BleoR (Addgene no. 1925081) was used for prime editing ...
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bioRxiv - Genetics 2022Quote: ... Jurkat cells containing pLenti-PE2-BSD (Addgene, #161514) were treated with 10 μg/ml blasticidin for selection of blasticidin and incubated for 72 hours ...
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bioRxiv - Genetics 2021Quote: ... 10 μg pCMV-PE2-P2A-GFP (Addgene, 132776) plasmid ...
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bioRxiv - Molecular Biology 2022Quote: ... Jurkat cells containing pLenti-PE2-BSD (Addgene, #161514) were treated with 10 µg/ml blasticidin for selection of blasticidin and incubated for 72 hours ...
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bioRxiv - Bioengineering 2023Quote: ... and pCMV-PE2 plasmids were obtained from Addgene (#105533 ...
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bioRxiv - Cell Biology 2024Quote: ... which were amplified from pCMV-PE2 (Addgene #132775), pCMV-PEmax-P2A-BSD (Addgene # 174821 ...
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bioRxiv - Molecular Biology 2022Quote: ... pCMV-PE2 was a gift from David Liu (Addgene plasmid # 132775). pLenti PE2-BSD was a gift from Hyongbum Kim (Addgene plasmid # 161514 ...
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bioRxiv - Genetics 2021Quote: Plasmids encoding PE2 (Addgene plasmid #13277519; http://n2t.net/addgene:132775; RRID:Addgene_132775) and AncBE4Max (Addgene plasmid #11209427 ...
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bioRxiv - Bioengineering 2021Quote: ... pCMV-PE2 was a gift from David Liu (Addgene plasmid # 132775) 6 ...
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bioRxiv - Molecular Biology 2022Quote: ... pLenti PE2-BSD was a gift from Hyongbum Kim (Addgene plasmid # 161514) (Kim et al. ...
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bioRxiv - Genetics 2020Quote: ... The Cas9 nickase-reverse transcriptase plasmid (pCMV-PE2, Addgene #132775, Watertown, MA) was linearized with PmeI (New England Biolabs #R0560S ...
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bioRxiv - Cancer Biology 2022Quote: ... pCMV-PE2 and pU6-pegRNA-GG-acceptor (Addgene #132775 and #132777, respectively)50 ...
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bioRxiv - Molecular Biology 2022Quote: ... CRISPR-Prime Editing system encoding lentiviral pLenti-PE2-BSD plasmid DNAs (Addgene, # 161514) encoding CRISPR-PE containing the blasticidin resistance gene ...
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bioRxiv - Cell Biology 2020Quote: ... 100,000 HT1-mCdHs were transfected with 900 ng of PE2-encoding plasmid (Addgene #132775), 300 ng of pegRNA-encoding plasmid ...
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bioRxiv - Molecular Biology 2022Quote: ... and Spy-ABE7.10 constructs were cloned into the pCMV-PE2 vector backbone (Addgene #132775) by Gibson assembly ...
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bioRxiv - Neuroscience 2022Quote: ... cells were co-transfected with 750 ng of pCMV-PE2 Plasmid (Addgene Plasmid #132775) and 250 ng of assembled pU6-pegRNA-GG-acceptor using Lipofectamine 2000 (Invitrogen) ...
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bioRxiv - Neuroscience 2022Quote: ... cells were co-transfected with 750 ng of pCMV-PE2 Plasmid (Addgene Plasmid #132775) and 250 ng of assembled pU6-pegRNA-GG-acceptor using Lipofectamine 2000 (Invitrogen) ...
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bioRxiv - Genomics 2023Quote: ... 2.5 ug of DNA (the pegRNA plasmid and the pCMV-PE2 plasmid (Addgene #132775) at a 1:2 ratio by mass of pegRNA plasmid:PE2) ...
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Therapeutic base and prime editing of COL7A1 mutations in recessive dystrophic epidermolysis bullosabioRxiv - Bioengineering 2021Quote: ... 150,000 patient-derived fibroblasts were transfected with 900 ng of PE2-encoding plasmid (Addgene #132775), 300 ng of pegRNA-encoding plasmid ...
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bioRxiv - Molecular Biology 2022Quote: ... cells in each well were transfected with 500ng pCMV-PE2 (Anzalone et al., 2009, Addgene#132775), 330ng pU6-pegRNA and 170ng pBPK1520-ngRNA for PE3 strategy or 500ng pCMV-PE2 ...
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bioRxiv - Molecular Biology 2023Quote: ... with 3 million K562 cells and 10 µg of pCMV-PE2-tagRFP-BleoR (Addgene no. 192508) per individual electroporation (Day 0) ...
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bioRxiv - Neuroscience 2024Quote: ... Plasmid based prime editing: 500 ng pCMV-PE2-GFP (a gift from David Liu, Addgene#132776)115 ...
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bioRxiv - Genetics 2023Quote: ... inserts were either ordered as gBlocks from Integrated DNA Technologies (IDT) or amplified from pCMV-PE2 (Addgene #132775) or pCMV-PEmax (Addgene #174820 ...
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bioRxiv - Biochemistry 2022Quote: The C-terminal P2A-EGFP sequence was added to SaABE8e or pCMV-PE2 (Addgene IDs 138500 and 132775, respectively), and the N-terminal BPNLS was added to an SpCas9 plasmid similar to pCMV-T7-SpCas9 (Addgene plasmid ID 139987 ...
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bioRxiv - Molecular Biology 2021Quote: ... was modified to contain the H840A mutation from pCMV-PE2 [1] (a gift from David Liu, Addgene plasmid # 132775) by EcoRV and PmlI fragment sub-cloning ...
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bioRxiv - Genetics 2020Quote: Prime Editor 2 (PE2) plasmid coding for the SpCas9 gene fused with the MLV reverse transcriptase was obtained from Addgene Inc ...
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bioRxiv - Genomics 2023Quote: ... We further replaced the Cas9 cassette with an nCas9/M-MLV-RT cassette from the pCMV-PE2 plasmid (Addgene, 132775). The lentiV2-pegRNA and lentiV2-ngRNA plasmids were constructed by replacing the Cas9 and Puromycin sequences in the lentiCRISPR v2 plasmid (Addgene ...
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bioRxiv - Genomics 2023Quote: The plasmids encoding PE2 (Plasmid#132775) as well as the plasmid for expressing the pegRNA (Plasmid #132777) were obtained from Addgene. PegRNAs were cloned into the plasmid backbone with paired oligonucleotides (IDT ...