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Citations for Addgene :
1 - 50 of 128 citations for PD L2 Cynomolgus HEK 293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Retrovirus were produced by transfecting HEK 293T cells with a mixture of PD-L1-pMIT1.1 (or PD-L2-pMIT1.1) and pCL-Eco (Addgene #12371) using Lipofectamine 3000 (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... for generation of bulk PD-L2-KO cells or luciferase (Addgene #105621) to monitor in vivo bioluminescence following standard procedures ...
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bioRxiv - Cell Biology 2020Quote: ... lentivirus was produced by transfecting HEK-293 cells with LV-RFP (Addgene 26001), pMD2.G (Addgene ...
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bioRxiv - Developmental Biology 2020Quote: ... HEK 293 cells were transfected with purified pCI-HA-YAP plasmid (Cat #27007, Addgene). DharmaFECT (Cat #T2002-02 ...
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bioRxiv - Microbiology 2022Quote: ... This was done by transfection of HEK-293 cells with an optimized T7 plasmid (Addgene 65974) together with the antigenomic plasmid ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... HEK-293 cells were transfected with two plasmid vectors CMV–R-GECO1 (a gift from Robert Campbell, Addgene plasmid #32444 ...
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bioRxiv - Plant Biology 2019Quote: ... into a L2 binary vector (AddGene #112207).
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bioRxiv - Cell Biology 2022Quote: ... Lentiviral particles were generated by transfection of HEK 293 cells (Cat.# ACC635; DSMZ, Braunschweig, Germany) with Pmd2 (AddGene, Plasmid #12259), lentiviral envelope plasmid psPAX2 (AddGene ...
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bioRxiv - Evolutionary Biology 2022Quote: ... L2 assembly: L1 modules and annealed oligonucleotides (8-9, Table S5) assembled into the L2 destination vector pAGM4723-Del (Addgene #112207). The final plasmids ...
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bioRxiv - Cancer Biology 2020Quote: Virus particles were generated in HEK 293-T cells after transfection with the above-mentioned plasmids in combination with the gag/pol plasmid psPAX2 (Addgene, 12260) and the VSV-g-envelope plasmid pMD2.G (Addgene ...
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bioRxiv - Developmental Biology 2023Quote: ... 100K-200K HEK 293 cells were reverse transfected with the M50 Super 8x TOPFlash plasmid (100 ng; Addgene #12456; gift from Randall Moon) and a construct constitutively expressing Renilla luciferase (5 ng ...
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bioRxiv - Cell Biology 2023Quote: ... pENTR L5-dec2WT-L2 and pENTR L5-dec2P384R-L2 plasmids were then individually combined with pENTR L1-13XLexAop2-R5 (Addgene #41433; (Petersen and Stowers, 2011)) and destination vector pDESTsvaw (Addgene #32318 ...
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bioRxiv - Bioengineering 2022Quote: ... Human PD-L1-GFP (pEGFP-N1/PD-L1) was a gift from Mien-Chie Hung (Addgene plasmid # 121478 ...
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bioRxiv - Microbiology 2021Quote: ... Derivatives of HEK-293T cells expressing ACE2 were generated by transducing HEK-293T (ATCC, CRL-3216) cells with ACE2 (Addgene, #145839). Cells were used as single cell clone was derived by limiting dilution from the bulk populations ...
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bioRxiv - Molecular Biology 2020Quote: ... The GFP nanobody-Fc fusion construct (pGEMHE-vhhGFP4-hIgG1-Fc; Addgene #105576) was described previously (Clift et al. ...
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bioRxiv - Bioengineering 2019Quote: Images of HEK cells co-expressing hGlut1 (Addgene #18085) and iGlucoSnFR.mRuby2 were acquired on an inverted Zeiss LSM 800 confocal microscope equipped with GaAsP detectors and an EC Plan-Neofluar 10x/0.30 M27 objective ...
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bioRxiv - Immunology 2020Quote: ... PD-L1 cDNA was cloned into pRRLSIN.cPPT.PGK vector (Addgene, 12252) with ClonExpress II One Step Cloning Kit (Vazyme ...
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Suppression of the Integrated Stress Response in Islet β Cells Decreases Risk of Autoimmune DiabetesbioRxiv - Cell Biology 2023Quote: Lipofectamine based transfections of 25 μg pEGFP-PD-L1 (Addgene) and GOLM1 (Origene ...
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bioRxiv - Cell Biology 2021Quote: ... The targeting vector containing a PGK-PuroTK cassette flanked by the corresponding homology arms was constructed by Gateway LR reaction using pENTR-L1-PGK-PuroTK-L2 (Addgene #141005), pUC-DEST-R3R4(R ...
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bioRxiv - Cell Biology 2021Quote: ... The targeting vector containing a PGK-Neo drug-resistance cassette flanked by the HPRT homology arms was constructed by Gateway LR reaction using pENTR-L1-PGK-Neo-L2 (Addgene #141007), pUC-DEST-R3R4(R ...
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bioRxiv - Neuroscience 2022Quote: ... and IgG2a Fc (Addgene #114492), were amplified and extracted similarly ...
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bioRxiv - Immunology 2021Quote: ... HEK cells were transfected with pMD2.G (Plasmid 12259; Addgene); psPAX2 (Plasmid 12260 ...
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bioRxiv - Cancer Biology 2020Quote: Lentiviruses were packaged in HEK 293T cells using psPAX2 (Addgene #12260) and pMD2.G (Addgene #12259 ...
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bioRxiv - Molecular Biology 2023Quote: ... Lentiviruses were generated by transfecting HEK-293T cells with pCMVR8.74 (RRID:Addgene_22036), pMD2G (RRID:Addgene_12259) ...
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bioRxiv - Immunology 2023Quote: ... Lentivirus was produced in HEK 293T using psPAX2 (Addgene plasmid # 12260) and VSV-G (Addgene plasmid # 12259 ...
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bioRxiv - Neuroscience 2022Quote: ... and mouse IgG1 Fc (Addgene #28216) and IgG2a Fc (Addgene #114492) ...
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bioRxiv - Biophysics 2021Quote: ... MDA-MB-231 cells were transfected with PD-L1-GFP (Addgene #121139) or PD-L1-GPF-C272A ...
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bioRxiv - Cell Biology 2019Quote: ... and transfected into HEK-293T cells together with pMD2.G (Addgene #12259) and psPAX2 (Addgene #12260 ...
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bioRxiv - Cancer Biology 2021Quote: ... HEK 293FT cells were transfected with pRK5-TGFβRI-FLAG plasmid (Addgene, #14833) using Lipofectamine 3000 transfection kit ...
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bioRxiv - Microbiology 2023Quote: ... HEK 293Ts were reverse-transfected with pcDNA3-AKT-PH-GFP (Addgene #18836) or pcDNA3-AKT-PH[R25C]-GFP (Addgene #18837) ...
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bioRxiv - Bioengineering 2024Quote: ... and then HEKs were transfected with envelope plasmid pMD2.G (Addgene #12259), packaging plasmids pMDLG/pRRE (Addgene #12251 ...
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Inhibition of nucleotide synthesis promotes replicative senescence of human mammary epithelial cellsbioRxiv - Systems Biology 2019Quote: Proliferating HMEC were infected at PD 14 with pLenti-PGK-hygro (Addgene 19066) encoding either hTERT or firefly luciferase ...
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bioRxiv - Systems Biology 2021Quote: ... proliferating HMECs were infected at PD 14 with pLenti-PGK-hygro (Addgene 19066) encoding either hTERT or firefly luciferase ...
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bioRxiv - Neuroscience 2022Quote: ... including the human IgG1 Fc (Addgene #145165), and mouse IgG1 Fc (Addgene #28216 ...
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bioRxiv - Cell Biology 2021Quote: ... and then transfected into HEK-293T cells together with pMD2.G (Addgene #12259) and psPAX2 (Addgene #12260) ...
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bioRxiv - Biochemistry 2021Quote: ... was packaged in HEK 293T cells using helper plasmids pMD2.G (Addgene #12259) and psPAX2 (Addgene #12260) ...
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bioRxiv - Cell Biology 2019Quote: ... Lentiviruses were generated in HEK 293T cells transfected with psPAX2 (Addgene plasmid 12260), pCMV-VSV-G (Addgene plasmid 8454 ...
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bioRxiv - Microbiology 2023Quote: ... was transfected into HEK-293T cells along with packaging plasmids pΔ8.74 (Addgene, 22036) and pMD2G (Addgene ...
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bioRxiv - Molecular Biology 2019Quote: Mouse SEMA6A-Fc and SEMA6c-Fc expression constructs were a gift from Woj Wojtowicz (Addgene plasmids 72163 and 72167, respectively) and human SEMA6A was gene-synthesized (GeneArt) ...
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bioRxiv - Molecular Biology 2019Quote: ... Mouse Sema6a-Fc and Sema6c-Fc expression constructs were a gift from Woj Wojtowicz (Addgene plasmids 72163 and 72167, respectively). Plasmid pHis1522 encoding his-tagged TcsL was synthesized and codon optimized for Bacillus megaterium (Genscript) ...
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bioRxiv - Biophysics 2023Quote: ... 3C protease site cloned into a pcDNA3 vector containing human IgG Fc derived from pcDNA3-Nrxn1beta AS4(-)-Fc (a gift from Peter Scheiffele & Tito Serafini, Addgene plasmid # 59313 ...
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Structure-guided glyco-engineering of ACE2 for improved potency as soluble SARS-CoV-2 decoy receptorbioRxiv - Biochemistry 2021Quote: ... United States).62,63 Plasmid constructs pcDNA3-sACE2(WT)-Fc(IgG1) and pcDNA3-sACE2-T92Q-Fc(IgG1) were obtained from Addgene (United States). The N322Q mutation was introduced into ACE2-wt-Fc and ACE2-T92Q-Fc using the QuikChange Lightning Site-Directed-Mutagenesis kit (Agilent Technologies ...
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bioRxiv - Immunology 2021Quote: ... HEK293 and HEK/TLR4 cells were transiently transfected with pcDNA3.1(-)hACE2 (Addgene plasmid #1786) to generate HEK/ACE2 or HEK/TLR4/ACE2 cell lines ...
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bioRxiv - Cell Biology 2020Quote: ... Lentiviral particles were produced in HEK-293T cells with the vectors psPAX2 (12259, Addgene), pMD2.G (12259 ...
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bioRxiv - Genetics 2022Quote: ... HEK- 293T cells were transfected with 100 ng pIS0 (Addgene #12178, encoding firefly luciferase), 100 ng of the Renilla reporter and 800 ng of empty vector (1 µg total plasmid DNA ...
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bioRxiv - Neuroscience 2019Quote: ... Lentivirus were generated using co-transfection into HEK-293t cells of psPAX2 (Addgene, 12260), pMD2.G (AddGene ...
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bioRxiv - Cell Biology 2023Quote: The producer cell line HEK 293TN was transfected with the packaging (psPAX2, Addgene 12260), envelope (pMD2.G ...
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bioRxiv - Physiology 2024Quote: HEK 293T were transfected with a human TLR3 plasmid or an empty vector (Addgene). Briefly ...
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bioRxiv - Microbiology 2024Quote: ACE2+ 293 were transiently transfected with pALPS expressing tat-p2a-rev cassette (Addgene, Watertown, MA, USA). The TZM.bl cell were transiently transfected with Wuhan CoV-2 spike expression plasmid pHDM (BEI resources ...
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bioRxiv - Genomics 2019Quote: Lentiviral vectors were produced by transfection of HEK 293T cells with the envelope (psPAX2, Addgene #12260), packaging (pMD2.G ...