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Citations for Addgene :
1 - 50 of 915 citations for P450 Demethylation Activity Kit 2 plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... The ER was marked using a Cytochrome p450 (CytERM) tagged to mScarlet (Addgene #85066), which was utilized as an ER marker ...
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bioRxiv - Genetics 2023Quote: ... and pX330S-2 (Addgene Kit 1000000055) according to the kit instructions ...
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bioRxiv - Microbiology 2021Quote: ... 2 x 105 cells were seeded on 12-well plates and transiently transfected with 2 μg of plasmids encoding SARS-CoV-2 N (Addgene # 141391) or eGFP (Addgene # 141395 ...
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bioRxiv - Microbiology 2020Quote: Cas9 activity was tested by transducing pXPR_011 (Addgene #59702), which expresses both GFP and sgRNA against GFP ...
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bioRxiv - Molecular Biology 2024Quote: Mouse Two Plasmid Activity-Optimized CRISPR Knockout Library (Addgene#1000000096) was amplified in E coli ...
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bioRxiv - Biochemistry 2020Quote: ... aureus with improved activity (eSrtA) was obtained from Addgene (plasmid #75144) and expressed in LB medium at 18°C for 16 hours (Chen et al. ...
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bioRxiv - Cell Biology 2019Quote: Reporters of enhancer activity were constructed by cloning mCerulean3 (Addgene #54730) into the multiple cloning site of Open Biosystems vector PB533A-2 ...
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bioRxiv - Genomics 2023Quote: ... Cas9 activity was evaluated using the fluorescence- based reporter pXPR011 (Addgene #59702), which expressed GFP and cognate sgRNA to assess GFP knockdown upon successful CRISPR activity ...
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bioRxiv - Cancer Biology 2019Quote: Cells were assessed for Cas9 activity using the pXPR_011 plasmid (Addgene plasmid # 59702)[63] according to protocol by Doench et al ...
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bioRxiv - Bioengineering 2021Quote: ... and screened for PE activity by transfecting CTT-inserting pegRNA alone (Addgene #132778) and sequencing the HEK3-target loci.
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bioRxiv - Physiology 2022Quote: ... PPARγ2 transcriptional activity was determined using the PPREx3-TK-luc reporter (Addgene, #1015).
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bioRxiv - Neuroscience 2023Quote: For pan-neuronal activity imaging 400nl of AAV1-Syn-GCaMP6s (Addgene #100843-AAV1) was injected unilaterally into the MPN of FVB/NJ mice ...
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bioRxiv - Neuroscience 2022Quote: ... which would lead to increased activity in projection neurons of the cortex (Addgene, 50474).
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bioRxiv - Cancer Biology 2024Quote: ... DHB-mVenus (CDK2 activity reporter) was a gift from Tobias Meyer & Sabrina Spencer (Addgene plasmid # 136461 ...
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bioRxiv - Cell Biology 2020Quote: ... 3 x 105 HeLa cells were seeded into a 6-well plate and next day transfected with 2 μg of the FLPe recombinase plasmid (Addgene #20733) (Beard et al. ...
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bioRxiv - Systems Biology 2019Quote: ... 350k HAP1 WT cells were seeded into a 6-well plate and 24 hours later cells were transfected with a mix of 2 µg pX459 plasmid (Addgene #62988) carrying a gRNA ...
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bioRxiv - Microbiology 2020Quote: ... Cas9 activity was checked using the XPR_047 assay (a gift from David Root, Addgene 107145) and was 79.5% and >83.4% ...
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bioRxiv - Biochemistry 2022Quote: Notch activity reporter (CBF:H2B-Venus) was a gift from Anna-Katerina Hadjantonakis (Addgene plasmid #44211) (Nowotschin et al. ...
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bioRxiv - Physiology 2023Quote: Circadian clock gene activity was recorded using the vectors pLV6-Bmal-luc (Addgene plasmid #68833) and pLV6-Per2-luc ...
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bioRxiv - Systems Biology 2024Quote: ... pPBbsr-JNKKTR-mCherry plasmid containing JNK activity reporter (JNK-KTR) was obtained from Addgene (#115493). mCherry in this plasmid was exchanged with iRFP713 (from Addgene #111510 ...
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bioRxiv - Neuroscience 2024Quote: The YAP-dependent transcriptional activity was assessed using an 8xGTIIC-luciferase construct (Addgene plasmid # 34615). Briefly ...
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bioRxiv - Cancer Biology 2024Quote: ... Efficient nuclease activity was confirmed using a reporter system (Addgene #67979, #67980; Supp. Fig. 1). We synthesized a custom single guide RNA (sgRNA ...
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bioRxiv - Biochemistry 2023Quote: ... MEFs were split into 6 well plates to ∼80% confluence and transfected with 2 µg SV40 1: pBSSVD2005 (a gift from David Ron; Addgene plasmid #21826) using FuGENE HD according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... WT Cas9 activity was checked using the XPR_047 assay (a gift from David Root, Addgene 107145) and was always >80-90% ...
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bioRxiv - Cancer Biology 2021Quote: ... Reporter assays to assess RUNX1 transcriptional activity were done using the pMCSF-R-luc plasmid (Addgene plasmid #12420 ...
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bioRxiv - Developmental Biology 2023Quote: ... CRIPSRi activity in each cell line was validated by lentiviral transduction with pCRISPRia-v2 (Addgene #84832) backbones containing either a guide RNA (gRNA ...
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bioRxiv - Biophysics 2019Quote: ... The enhanced activity SrtA pentamutant was expressed and purified from bacteria using the pET29-eSrtA vector (Addgene, #75144) as previously reported 31 ...
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bioRxiv - Cancer Biology 2021Quote: ... Reporter assays to assess RUNX1 transcriptional activity were done using the pMCSF-R-luc plasmid (Addgene plasmid #12420; http://n2t.net/addgene:12420; RRID:Addgene_12420) (Zhang et al. ...
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bioRxiv - Genetics 2020Quote: ... Cas9 activity was assessed by transducing parental Vero-E6 or Vero-E6-Cas9 cells with pXPR_047 (Addgene 107645), which expresses eGFP and an sgRNA targeting eGFP (72) ...
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bioRxiv - Cancer Biology 2024Quote: ... DHB-mVenus (CDK2 activity reporter) was a gift from Tobias Meyer & Sabrina Spencer (Addgene plasmid # 136461; http://n2t.net/addgene:136461; RRID:Addgene_136461) (27).
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bioRxiv - Synthetic Biology 2023Quote: ... Vectors for arabinose memory (pAra) and the GFP reporter of recombinase activity (pRec1-OFF) were obtained from Addgene (40). In pAra ...
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bioRxiv - Neuroscience 2023Quote: ... we first labeled odor responsive neurons with the permanent activity marker FLEX-RAM (Addgene # 84468, (Sørensen et al., 2016)) in-vivo ...
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bioRxiv - Neuroscience 2024Quote: ... A genetically encoded calcium indicator for the detection of ACh activity (1 μL, AAV9-hSyn-ACh3.0; AddGene; Watertown, MA) was infused into the medial prefrontal cortex (AP(2.7mm) ...
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bioRxiv - Cancer Biology 2021Quote: ... Cas9 expression was confirmed by immunoblot analysis and Cas9 activity was confirmed using a Cas9 GFP reporter [(pXPR_011 (Addgene #59702)] that showed near maximal editing 10 days after infection ...
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bioRxiv - Cell Biology 2022Quote: Three different dCas9 fusion proteins were tested for dCas9 activity in both K562 and hiPSC lines: pLX_311-KRAB-dCas9 (Addgene 96918), pB-CAGGS-dCas9-KRAB-MeCP2 (Addgene ...
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bioRxiv - Cell Biology 2023Quote: ... In vitro target specific gRNA cleavage activity was validated by transfecting N2A cells with PCR amplified gRNA gblock and Cas9 plasmid DNA (px330, Addgene) using ROCHE Xtremegene HP ...
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bioRxiv - Cancer Biology 2023Quote: Autophagy degradative activity (autophagic flux) was measured using an expression vector encoding the fusion protein mCherry-EGFP-LC3B (Addgene, #22418). We transfected JHU 011 cell line with pBabe-mCherry-EGFP-LC3B vector and established a stable cell line by puromycin selection ...
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bioRxiv - Genetics 2020Quote: A pair of TALENs targeting zebrafish tnfaip3 (A20) exon 2 were generated using “PLATINUM Gate TALEN Kit” (Addgene, #1000000043). 0.2 ng of mRNA encoding the TALEN pair was delivered into the cytoplasm of wild-type zebrafish embryos at the one-cell stage ...
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bioRxiv - Bioengineering 2022Quote: ... with 2 μg MLC-2 plasmid (pEGFP-MRLC1, Addgene, #35680), 10 μg RAR-β siRNA (Santa Cruz ...
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bioRxiv - Neuroscience 2023Quote: ... virus for optogenetic inhibition or excitation of preBötCOT-R neurons in OT-R::Cre mice was obtained from Addgene (Table 2, Addgene viral prep # 154951 ...
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bioRxiv - Neuroscience 2023Quote: ... The AAV1-phSyn1(S)-FLEX-tdTomato-T2A-SypEGFP-WPRE virus for tracing experiments from preBötCOT-R neurons to nACardiac neurons in OT-R::Cre mice was obtained from Addgene (Table 2, Addgene viral prep # 51509 ...
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bioRxiv - Cell Biology 2020Quote: Approximately 300 × 106 IAPEz reporter cells expressing Cas9 were lentivirally infected with a genome-wide Mouse Two Plasmid Activity-Optimized CRISPR Knockout Library (Addgene #1000000096) as described above ...
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bioRxiv - Microbiology 2023Quote: ... or with the packaging plasmid pCD/NL-BH*deltavpu/RT-that lacks RT activity due to the D110E mutation in the catalytic site (kindly provided by Jakob Reiser; Addgene #136985). CA stability mutants P38A and E45A were kindly provided by Stephen Goff ...
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bioRxiv - Cancer Biology 2023Quote: ... 377 million iCas9-expressing NIH-3T3 cells were transduced with the Mouse Two Plasmid Activity-Optimized CRISPR Knockout Library (Wang et al, 2017) (Addgene; 1000000096) using spinfection at an MOI of 0.3 to ensure a final library coverage of 500X ...
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bioRxiv - Cancer Biology 2024Quote: ... Single clones were obtained by fluorescence-activated cell sorting and functionally tested for Cas9 activity using a lentiviral reporter pKLV2-U6gRNA5(gGFP)-PGKBFP2AGFP-W (Addgene #67980). PPM1D-mutant cell lines were generated using the RNP-based CRISPR/Cas9 delivery method using a single sgRNA (GCTAAAGCCCTGACTTTA) ...
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bioRxiv - Genomics 2020Quote: HeLa SCC1-AID cells stably expressing dCas9-KRAB for CRISPRi-mediated repression of enhancer or promoter activity were generated by transduction with the lentiviral vector pHR-SFFV-KRAB-dCas9-P2A-mCherry (Gilbert et al., 2014) (Addgene plasmid #60954) and subsequent sorting for mCherry-positive cells using a FACS Aria III cell sorter (BD Lifesciences) ...
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bioRxiv - Neuroscience 2021Quote: ... AAV2/2 (Addgene 104963), adenovirus helper plasmid pAdDeltaF6 (Addgene 112867 ...
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bioRxiv - Microbiology 2020Quote: ... The plentiCRISPRV.2 (Addgene) was digested with BsmBI and ligated with guide RNA sequences specific for IFNAR1 ...
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bioRxiv - Neuroscience 2023Quote: ... pAAV2/2 (Addgene #104963), pAAV2/5 (Addgene #104964) ...
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bioRxiv - Biophysics 2019Quote: ... A transgenic line with a GCaMP6s probe reporting Ca2+ activity we generated in the Kiel center using a modified version of the pHyVec1 plasmid (Addgene catalog no. 34789) which replaces the GFP sequence with a GCaMP6s sequence that was codon-optimized for Hydra ([35]) ...