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Citations for Addgene :
1 - 30 of 30 citations for Nuclease, Micrococcal since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... 700ng/mL of proteinA-micrococcal nuclease (pA-Mnase purified in house with vector from Addgene 86973 79) were incubated with the nuclei at 4 degrees for an hour ...
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bioRxiv - Molecular Biology 2021Quote: ... Cas9 nuclease (Addgene #1074181), tracrRNA (Addgene #1072532) ...
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bioRxiv - Genetics 2019Quote: ... Nucleases were amplified from Addgene plasmids indicated in Supplemental Table S1 ...
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bioRxiv - Neuroscience 2021Quote: ... 7 μg Cas9 nuclease plasmid (pX459, Addgene #62988) 1.4 μg pPN298 and 1.4 μg pPN306 were electroporated into 2.5×106 cells at 1050V ...
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bioRxiv - Genomics 2021Quote: ... nuclease-deficient dCas9-KRAB-MeCP2 (Addgene plasmid no. 110821) was stably introduced in HEK293T cells using the piggy-transposase system (System Biosciences ...
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A human cancer cell line initiates DNA replication normally in the absence of ORC5 and ORC2 proteinsbioRxiv - Molecular Biology 2020Quote: ... Human codon optimized Cas9 nuclease (hCas9) was used (Addgene (41815)) ...
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bioRxiv - Neuroscience 2021Quote: ... Dox-inducible CRISPR nuclease (CRISPRn) plasmid (pAAVS1-PDi-CRISPRn, Addgene) was used as a starting material ...
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bioRxiv - Cell Biology 2021Quote: ... the equivalent nuclease-deficient Cas9 was obtained from Addgene (#72269). All plasmids were verified by DNA sequencing.
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bioRxiv - Genetics 2023Quote: The CMV-driven SaCas9 nuclease vector pX60143 (Addgene plasmid #61591) was a gift from Feng Zhang (Massachusetts Institute of Technology) ...
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bioRxiv - Neuroscience 2022Quote: ... and humanised Cas9 nuclease vector (a gift from George Church, Addgene plasmid # 41815 ...
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bioRxiv - Genomics 2019Quote: ... the gRNA expression plasmid AX03_pgEGFP and the hCas9 nuclease plasmid (Addgene plasmid 41815) were used for generating DSBs at the EGFP sequence ...
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bioRxiv - Physiology 2021Quote: Production of Cas9 nuclease mRNA utilized the pMLM313 expression vector (Addgene plasmid #42251), which harbors the T7 promoter site upstream of the translational start site ...
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bioRxiv - Cell Biology 2022Quote: ... parental HT1080 cells expressing stable nuclease-dead Cas9 (dCas9-BFP-KRAB, Addgene # 46911) were used as described previously23 ...
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bioRxiv - Synthetic Biology 2021Quote: The nuclease-deficient Cas9 was obtained from pHR-SFFVdCas9-BFP-KRAB (dCas9; plasmid 46911, Addgene). The gRNA containing MS2 aptamer sequences (gRNA_2 ms2 ...
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bioRxiv - Genetics 2023Quote: ... selected SaCas9 sgRNAs were cloned into the TBG-driven SaCas9 nuclease rAAV vector pX60243 (Addgene plasmid #61593 ...
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bioRxiv - Cancer Biology 2024Quote: ... Efficient nuclease activity was confirmed using a reporter system (Addgene #67979, #67980; Supp. Fig. 1). We synthesized a custom single guide RNA (sgRNA ...
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bioRxiv - Molecular Biology 2022Quote: ... yeast expression constructs for nuclease-inactivated Cas9 (D10A/H840A mutations) from Streptococcus pyogenes –dCas9 (Addgene #46920) (Gilbert et al 2013 ...
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bioRxiv - Developmental Biology 2021Quote: ... AAAC-CCTGAGCAGAAGAGTGGTTA-C) were designed and ligated into the RNA-guided nuclease plasmid (pX330-mCherry plasmid; Addgene), in order to induce the Cas9-mediated DSB in the genomic DNA of the Dystrophin-deficient iPSCs ...
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bioRxiv - Cell Biology 2022Quote: ... The sgRNA oligos (Table S2) were introduced into pX458 expressing Cas9 nuclease fused to GFP (Addgene #48138). pSpCas9(BB)-2A-GFP (PX458 ...
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bioRxiv - Biophysics 2023Quote: ... Transfections contained 70 ng of SpCas9 nuclease plasmid (pCMV-T7-SpCas9-P2A-EGFP (RTW3027; Addgene ID 139987) and 15 ng each of two sgRNA expression plasmids mixed with 0.3 µL of TransIT-X2 (Mirus ...
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bioRxiv - Molecular Biology 2020Quote: ... AsCas12a and SpCas9 nucleases were expressed from the pET-based T7 promoter-containing plasmids (Addgene, Plasmids #90095, #62374) in Escherichia coli strain BL21-DE3 as described (3 ...
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bioRxiv - Neuroscience 2022Quote: ... and humanised Cas9 nuclease vector (a gift from George Church, Addgene plasmid # 41815 ; http://n2t.net/addgene:41815 ; RRID:Addgene_41815) (Mali et al. ...
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bioRxiv - Genetics 2023Quote: ... following the manufacturer’s protocol with AAVS1 targeting vector and predesigned transcription activator-like effector nucleases (hAAVS1 TALEN Left and Right were gifts from Su-Chun Zhang, Addgene plasmid # 52341 & 52342 ...
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bioRxiv - Biochemistry 2024Quote: ... expressing Cas9 nuclease and two gRNAs (see below for sequences) together with the CRIS-PITCh vector pX330S-2-PITCh (63670, Addgene), harboring the Lamin A microhomologies and GFP-Puro or GFP-Neo/Kan insertions ...
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bioRxiv - Molecular Biology 2019Quote: The gene encoding full length Francisella novicida (Fn) Cas9 nuclease residues 1-1629 bp) was PCR amplified using PX408 (Addgene 68705) as a template and cloned in pET28-His-10-Smt3 vector (a kind gift from Prof ...
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bioRxiv - Genetics 2020Quote: Human codon-optimized high fidelity Cas9 nuclease construct with GFP tag (pSpCas9(BB)-2A-GFP (PX458))80 was obtained from Addgene (#48138). Two sgRNAs were designed using CRISPR-Cas9 guide RNA design checker from Integrated DNA Technologies ...
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bioRxiv - Cancer Biology 2019Quote: The B-cell acute lymphoblastic leukemia cell lines NALM-6 and REH were lentivirally transduced with plasmid expressing Cas9 nuclease (LentiCRISPR V2; Addgene Plasmid #52961) as described below at a MOI of 0.5 ...
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bioRxiv - Developmental Biology 2021Quote: The p133-pPB plasmids encoding for the Tal1-gRNAs were co-transfected with the pX458 plasmid containing a Cas9 nuclease construct and a GFP reporter (pSpCas9-2A-GFP, Addgene ID: 48138), kindly provided by Jamie Hackett from EMBL Rome ...
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bioRxiv - Cancer Biology 2020Quote: ... Cell lines stably expressing the Cas9 nuclease were generated by infection with the empty lentiCRISPRv2 lentivirus (Addgene, #52961, not containing an sgRNA guide) using standard methods (https://www.addgene.org/viral-vectors/lentivirus/lenti-guide/) ...
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bioRxiv - Cell Biology 2023Quote: ... vectors encoding a pair of AAVS1-specific zinc finger nucleases (AAVS1-ZFN-L and AAVS1-ZFN-R) and an AAVS1 Neo-CAG-rtTA donor template were obtained from Addgene (plasmids #60915, #60916, and #60431). For transfection ...