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Citations for Addgene :
1 - 50 of 1353 citations for Mono 2 Carboxymethyl Hexyl Phthalate Cp 95% 13C4 99% Dehp Metabolite Iv 100Ug Ml In Mtbe since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Membrane-dependent actin polymerization mediated by the Legionella pneumophila effector protein MavHbioRxiv - Molecular Biology 2023Quote: Bacterial expression of CP plasmid was purchased from Addgene (Plasmid #89950). The α subunit of CP was subcloned into a pRSFDuet-based vector in frame with an N terminal His-Sumo tag and the β subunit of CP was subcloned into a pCDFuet-based vector ORF2 ...
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bioRxiv - Neuroscience 2023Quote: ... α-syn 1-95 (Addgene #213499), α-syn 1-110 (Addgene #213500) ...
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bioRxiv - Neuroscience 2020Quote: ... The (CGG)99 plasmid was obtained from Addgene and the (CTG)202 plasmid was a kind gift from Maurice Swanson ...
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bioRxiv - Physiology 2021Quote: ... or PSD-95-pTagRFP (Addgene #52671), and cloned in using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs ...
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bioRxiv - Neuroscience 2023Quote: ... and pCAG_FingR.GFP-PSD-95-CCR5TC (Addgene 46295) were used to amplify the coding region with additional 5’ zinc finger binding sequence to regulate their expression20 ...
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bioRxiv - Developmental Biology 2020Quote: ... pJFRC19-13XLexAop2-IVS-myr::GFP (Addgene_26224), was identically digested to remove myr::GFP ...
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bioRxiv - Physiology 2021Quote: ... 100 U/ml penicillin and 100 ug/ml streptomycin in a 5% CO2/95% air atmosphere at 37C Plasmid GP-CMV-GCaMP6s (Addgene plasmid # 40753) was cloned into lentiviral vector pCDH-EF1-MCS-IRES (puro ...
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bioRxiv - Physiology 2022Quote: pJFRC2-10XUAS-IVS-mCD8::GFP (Addgene #26214) plasmid was digested with NotI and XbaI ...
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bioRxiv - Genetics 2022Quote: ... and pJFRC19-13XLexAop2-IVS- myr::GFP (Addgene #26224) or 13XLexAop-IVS-myr::tdTomato (this study ...
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bioRxiv - Neuroscience 2021Quote: ... which was replaced by a Hsp70-IVS fragment excised by AatII and NotI from the plasmid pJFRC28-10XUAS-IVS-GFP-p10 (Addgene #36431). The sequence of the final product (15XQUAS-Hsp70-IVS-Syn21-GCaMP6f (codon-optimized)-p10 ...
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bioRxiv - Cell Biology 2021Quote: 1OxUAS-IVS-Nox::YPet was generated by using the pJFRC12-10XUAS-IVS-myr-GFP vector (Addgene 26222; Pfeiffer et al., 2010) as a backbone which was modified by substituting GFP with YPet (Nguyen and Daugherty ...
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bioRxiv - Developmental Biology 2021Quote: Tol2-enhanced green fluorescent protein (EGFP)-C1 was prepared by digesting pT2-7xTcf-NLS-CNL-CP (a gift from Yasushi Okada (Addgene plasmid #65715), Takai et al ...
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bioRxiv - Plant Biology 2022Quote: ... the CP or MP fragments were cloned into vector L4440gtwy that includes attR sites flanked by T7 promoters (G. Caldwell, Addgene plasmid # 11,344) using LR clonase (Thermo Scientific ...
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bioRxiv - Genomics 2023Quote: ... pJFRC12-10XUAS-IVS-myr::GFP plasmid (Addgene, Plasmid #26222) (Pfeiffer et al. ...
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bioRxiv - Cell Biology 2020Quote: ... Blunt-end repaired RhoBAST16 was cloned into 5′ UTR CGG 99× FMR1-EGFP (Addgene, plasmid #63091) which was digested with the NotI enzyme ...
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bioRxiv - Neuroscience 2023Quote: ... AAV1-hSyn-GCaMP7b (2 × 1013 vg/mL Penn Vector Core/Addgene; diluted 1:2 in saline) for imaging of pulvinar axons ...
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bioRxiv - Neuroscience 2021Quote: ... AAV1-flex-ChR2-Tdtomato (Addgene, titer 2*1012/ml, 0.5 μl) or AAV1-flex-ChR2-mCherry (Charite Vector core ...
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bioRxiv - Neuroscience 2023Quote: ... 1:2 dilution) or AAV5-pCAG-FLEX-EGFP-WPRE (1.1 x 1013 gc/ml, Addgene; 1:2 dilution) was injected in VTA (from bregma ...
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bioRxiv - Neuroscience 2020Quote: ... pJFRC19 (harboring 13XLexAop2-IVS, referring to the plasmid Addgene Cat# 26224) to generate constructs pUAST-myr::GCaMP5a ...
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bioRxiv - Neuroscience 2023Quote: ... a viral vector for the expression of the genetically encoded calcium indicator GCaMP6f (pAAV.Syn.GCaMP6f.WPRE.SV40, Cat. Number #100837-AAV1, titre 1.3 x 1013, Addgene 95) was injected stereotaxically (Kopf ...
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bioRxiv - Neuroscience 2024Quote: ... AAV8-hSynapsin1-hM3D(Gq)-mCherry (Addgene, #50474, ≥ 2 x 1012 vg/ml) or AAV9-hSynapsin1-Lamp1-mScarlet-I (3.06 x1012 vg/ml ...
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bioRxiv - Neuroscience 2020Quote: ... AAV1.hSyn.GCaMP6f.WPRE.SV40 (120 nl, 2×1013 vg/mL Penn Vector Core/Addgene; diluted 1:2 to 1:10 in saline) was injected either into V1 ...
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bioRxiv - Neuroscience 2020Quote: ... pJFRC19-13xlexAop2-IVS-myr::GFP was from Gerald Rubin (Addgene plasmid #26224). Site-specific transgenic flies were generated using standard methods (Bestgene ...
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bioRxiv - Neuroscience 2022Quote: ... pJFRC7-20XUAS-IVS-mCD8::GFP was a gift from Gerald Rubin (Addgene plasmid # 26220 ...
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bioRxiv - Neuroscience 2021Quote: ... Pups were injected bilaterally with 2 μl of AAV9-hsyn-ACh3.0 (1.8×10^13 gc/ml) and 2 μl of AAV9-hsyn-NES-jRCaMP1b (2.5×10^13 gc/ml, Addgene) per hemisphere ...
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bioRxiv - Neuroscience 2019Quote: ... 5.2×1012 gc/ml) and AAV2/5-hSyn-DIO-hM3Dq-mCherry (7.8×1012 gc/ml) were produced from Addgene plasmids #44362 and #44361 at the facility of Nantes University (UMR 1089 ...
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bioRxiv - Genomics 2022Quote: sgRNA targeting the genomic region of integration was cloned in BbsI linearized pX335-U6-Chimeric_BB-CBh-hSpCas9n (99) (D10A) plasmid (Addgene #42335) using NEBuilder HiFi DNA Assembly Master Mix (1:3 molar ratio ...
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bioRxiv - Neuroscience 2020Quote: ... This was then cloned into a linearized 20xUAS-IVS-mCD8::GFP (Addgene #26220) (digested with XhoI and XbaI ...
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bioRxiv - Genetics 2022Quote: ... The different combinations were cloned into pJFRC7-20XUAS-IVS-mCD8::GFP (Addgene # 26220) cut with XhoI (NEB catalogue # R0146S ...
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bioRxiv - Developmental Biology 2022Quote: ... The IVS and p10 fragments were derived from the vector pJFRC81 (Addgene #36432), Syn21 sequence (Pfeiffer et al. ...
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bioRxiv - Neuroscience 2020Quote: ... B3 recombinase was amplified from pJFRC157-20XUAS-IVS-B3::PEST27 (Addgene plasmid #32136) using primers to add a syn21 translational enhancer sequence and remove the PEST domain ...
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bioRxiv - Neuroscience 2023Quote: ... myrGFP from pJFRC19-13XLexAop2-IVS-myr::GFP (gift from Gerald Rubin, Addgene #26224) (Pfeiffer et al. ...
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bioRxiv - Neuroscience 2024Quote: ... and inserted into the pJFRC81-10xUAS-IVS-Syn21-GFP-p10126 vector (Addgene #36432) to replace the GFP sequence using NEBuilder HiFi DNA assembly master mix (New England Biolabs).
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bioRxiv - Neuroscience 2022Quote: ... AAV vectors expressing ChrimsonR (pAAV1-Syn-ChrimsonR-tdT; 2 × 10^13 GC/ml; Addgene) 27 or channelrhodopsin-2 (hChR2 ...
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Microbial signals and lymphotoxin drive TNF-independent death of A20 and ABIN-1 deficient epitheliumbioRxiv - Immunology 2021Quote: ... followed by a GSG linker and mouse IgG2a Fc fusion (amino acid 99-330; UniProtKB/Swiss-Prot P01863) were synthesized and then cloned into pENTR (Addgene Plasmid #17398) using InFusion cloning according to manufacturer’s instructions (Clontech) ...
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bioRxiv - Biochemistry 2021Quote: ... DNA encoding CGI-99 (Uniprot Q9Y224) was cloned into site 1 of the UC Berkeley MacroLab 5A vector (gift from Scott Gradia, Addgene plasmid #30121), while DNA sequence encoding FAM98B (Uniprot Q52LJ0 ...
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bioRxiv - Biochemistry 2021Quote: DNA encoding amino acids Phe2-Asp101 of human CGI-99 was cloned into the UC Berkeley MacroLab 1B vector (Addgene plasmid # 29653). The fusion protein containing an N-terminal His6 tag and a TEV protease cleavage site was expressed in BL21 (DE3 ...
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bioRxiv - Cancer Biology 2020Quote: ... The PCR product was subcloned into pJFRC19-13XLexAOP-IVS-myr::GFP vector (Addgene#26224) to generate pJFRC19-13XLexAOP-yki3S/A ...
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bioRxiv - Molecular Biology 2021Quote: ... synthesized de novo and cloned into the 20XUAS-IVS-Jaws-mVenus_tr plasmid (Addgene #111553) by swapping the Jaws-mVenus gene.
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bioRxiv - Developmental Biology 2021Quote: ... and 2.5 ng/mL Pmyo-2::mCherry as a co-injection marker (pCFJ90, Addgene #19327). Microinjection of adult N2 hermaph-rodites was performed as described above ...
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bioRxiv - Molecular Biology 2022Quote: ... a 2 mL transfection mixture containing lentiviral packaging plasmids 7.5 µg pMD2.G (#12259, Addgene), 11.4 µg pMDLg-RRE (#12251 ...
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bioRxiv - Neuroscience 2023Quote: ... Retro-AAV2 coding for mCherry (pAAV2-hSyn-mCherry, Addgene #114472, 2 x 1013 GC/ml) and green fluorescent protein (pAAV2-hSyn-eGFP ...
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bioRxiv - Microbiology 2022Quote: ... 500 ml LB culture of pCDFDuet-1-6×His-SARS-CoV-2-NSP7/NSP8 (Addgene)-transformed E.coli BL21 DE3 strain was induced by isopropyl β-d-1-thiogalactopyranoside (IPTG ...
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bioRxiv - Neuroscience 2019Quote: ... We used PCR to amplify GFP from pJFRC81-10XUAS-IVS-Syn21-GFP-p10 (JFRC, Addgene) and added an AgeI site to the reverse primer ...
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bioRxiv - Cell Biology 2021Quote: ... The PCR products were then inserted in the pJFRC4-3XUAS-IVS-mCD8::GFP (Addgene 28243) linearized by NotI ...
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bioRxiv - Neuroscience 2022Quote: ... (4) P10-3’UTR was amplified from pJFRC81-10XUAS-IVS-Syn21-GFP-p10 (Addgene 36432); (5 ...
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bioRxiv - Neuroscience 2024Quote: ... the fragments were cloned into an pJFRC7-20XUAS-IVS-mCD8::GFP vector (Addgene plasmid # 26220), replacing the mCD8::GFP insert via restriction enzyme digest (XhoI ...
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bioRxiv - Neuroscience 2021Quote: ... and AAV.Syn.NES-jRGECO1a.WPRE.SV40 (Serotype 2/9; titer ≥ 1×1013 vg/mL) viruses were purchased from Addgene.
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bioRxiv - Neuroscience 2023Quote: 750 nl of rAAV.EF1a.DIO.hChR2(H134R).eYFP or rAAV.EF1a.DIO.eYFP (3-4 x 10^12 vg/ml, AAV5, University of North Carolina Vector Core; 1-2 x 10^13 vg/ml, AAV1, Addgene, 27056-AAV1 and 20298-AAV1) were injected into each hemisphere of the VTA of 3–4-month-old DAT-Cre mice ...
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bioRxiv - Cancer Biology 2020Quote: ... or with 2 μg/ml puromycin (Gibco) (pTK93_Lifeact-mCherry; a gift from Iain Cheeseman, Addgene plasmid #46357) for 3 days ...