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Citations for Addgene :
1 - 50 of 190 citations for Lactose operon repressor LacI E.coli His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2024Quote: ... pPEPY-PF6-lacI (Addgene plasmid # 85589) and pJWV102-PL-dCas9 (Addgene plasmid # 85588 ...
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bioRxiv - Developmental Biology 2024Quote: ... the dCas9-KRAB-meCP2 repressor (Addgene 110821)55 was used ...
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bioRxiv - Synthetic Biology 2019Quote: ... which has constitutive levels of LacI and TetR repressors39 (Addgene).
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bioRxiv - Biochemistry 2023Quote: The construct for E.coli expression of WT caspase-3 (pET23b-Casp3-His) was obtained from Addgene (plasmid 11821). The construct for mammalian expression of caspase-3/GFP was a gift from Dr ...
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bioRxiv - Developmental Biology 2020Quote: ... pBabe-Puro-IKBalpha-mut (super-repressor) (dominant negative mutant) (Addgene 15291) and its control pBabe-Puro-IKBalpha-WT (Addgene 15290 ...
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bioRxiv - Cell Biology 2022Quote: ... human ubiquitin C-driven CymR Cuo repressor purchased from Addgene (#119907) into pPig-Hygro transposase backbone from Max Wilson ...
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bioRxiv - Cancer Biology 2023Quote: E.coli DH5alpha with pBudORF1 (Addgene) and pBudORF2 (Addgene ...
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bioRxiv - Biochemistry 2019Quote: ... This lac operon sequence was ligated into px458 (Addgene plasmid ID: 42230) to construct px458-lac ...
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bioRxiv - Synthetic Biology 2023Quote: ... The pNL29 operon was first cloned from the pST39-pNL29 plasmid (Addgene ID 91696), and the ORF of GvpK overlapping the ORF of GvpS was resolved by moving the start codon of GvpK to the downstream of GvpS ...
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bioRxiv - Cell Biology 2022Quote: ... followed in frame with coding sequence for LacI-NLS (sequence taken from “Cherry-LacRep”; plasmid #18985; Addgene). This sequence was subcloned in the pCDH-EF1-CymR-T2A-Puro (QM200VA-1 ...
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bioRxiv - Immunology 2022Quote: ... coli strain harboring a plasmid with the ilux operon (ilux pGEX(−)) was obtained from Addgene (plasmid # 107897 ...
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bioRxiv - Microbiology 2022Quote: ... Early passage HEK293T cells were transfected with 1.5 μg of dCas9-KRAB-MeCP2 repressor plasmid (Addgene #110824) and 0.5 μg of Piggyback Transposase (gift from Maxim Greenberg) ...
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bioRxiv - Microbiology 2023Quote: ... Fragments of the violacein operon were cloned from pBLxVi5 (a gift from Christopher Reisch, Addgene #167516) into pInt_attP1_kanR (26) ...
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bioRxiv - Cell Biology 2022Quote: ... and the constitutive tetracycline repressor A3 mutant expressing pLenti-CMV-rtTA3 (gift from Eric Campau (Addgene plasmid # 26429) to infect AoSMC cells as described in (Kubben et al. ...
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bioRxiv - Microbiology 2023Quote: ... An inducible GFP was made by inserting the Yersinia operon 1 promoter sequence into plasmid pFCcGi (Addgene) upon restriction with HindIII and XbaI (NEB) ...
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bioRxiv - Bioengineering 2023Quote: ... near a Cas9 fused tracrRNA-crRNA fusion to replace the RFP and LacI genes in a PCR’ed fragment of pBbS2K-RFP (Addgene #35330). The Golden Gate assembly cassettes of the resulting plasmid (called pSECRETS-B ...
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bioRxiv - Cell Biology 2024Quote: ... mCherry-FokI and the catalytically dead mCherry-FokI (D540A) cDNAs were subcloned into the mCherry-LacI vector (Addgene plasmid #18985) replacing mCherry ...
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bioRxiv - Synthetic Biology 2019Quote: ... The negative transcriptional feedback circuit used for the experiment contained a transcriptional repressor protein TetR expressed under a self-repressible promoter (Addgene plasmid # 45774; http://n2t.net/addgene:45774; RRID:Addgene 45774). TetR was fused to the green fluorescent protein variant deGFP and measured using excitation and emission at wavelengths 485 nm and 525 nm ...
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bioRxiv - Neuroscience 2022Quote: ... Err2EnR was generated by fusing the open reading frame of the transcriptional repressor domain of Drosophila Engrailed (amplified from CAG-EnR plasmid, Addgene plasmid #19715 ...
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bioRxiv - Synthetic Biology 2019Quote: ... The negative transcriptional feedback circuit used for the experiment contained a transcriptional repressor protein TetR expressed under a self-repressible promoter (Addgene plasmid # 45774 ...
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bioRxiv - Cell Biology 2019Quote: ... The cells were transduced first by lentivirus expressing dCas9 fused to the KRAB repressor module9 (pHR-SFFV-dCas9-BFP-KRAB, Addgene). Lentivirus-expressing cells were sorted (FACSAria II ...
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bioRxiv - Genomics 2023Quote: ... the DNA sequence of KRAB repressor domain was amplified by PCR from the pHAGE TRE dCas9-KRAB (Addgene plasmid #50917) and cloned in frame into the PB-TRE-dCas9-VPR backbone (Addgene plasmid #63800 ...
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bioRxiv - Neuroscience 2020Quote: ... pEZYmyc-His (Addgene plasmid #18701 ...
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bioRxiv - Molecular Biology 2021Quote: ... The CRISPRi repressor dCas9-KRAB-MECP2 (Yeo et al., 2018) was a gift from Alejandro Chavez and George Church (Addgene 110821). The backbones for the lentiviral vector pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-GFP and pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-puro (Thakore et al. ...
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bioRxiv - Cancer Biology 2023Quote: ... the EF-1α core promoter and the tetracycline repressor (TetR) into the KpnI and BamHI sites of lentiCRISPR-v2 (Addgene, 52961). Then ...
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bioRxiv - Bioengineering 2023Quote: ... Plasmid for E.Coli expression of ABE (Addgene #161788 from the Liu lab) was modified to express the ABE variant ABE8-13m (28 ...
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bioRxiv - Synthetic Biology 2019Quote: ... The lacZ deficient (lac operon deletion) BL21-Gold-dLac (DE3) strain was a gift from Jeff Hasty (Addgene plasmid # 99247).9 The exception to this was the AtzB-enriched extract ...
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bioRxiv - Synthetic Biology 2019Quote: ... The LacZ deficient (lac operon deletion) BL21-Gold-dLac (DE3) strain was a gift from Jeff Hasty (Addgene plasmid # 99247).29
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bioRxiv - Neuroscience 2022Quote: ... Err2EnR was generated by fusing the open reading frame of the transcriptional repressor domain of Drosophila Engrailed (amplified from CAG-EnR plasmid, Addgene plasmid #19715, Addgene, Watertown, USA) to Err2.
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bioRxiv - Cancer Biology 2020Quote: ... Human DLK1 ectodomain expression vector was obtained from Addgene (DLK1-bio-His, RRID:Addgene_51876) (Sun et al. ...
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bioRxiv - Cell Biology 2020Quote: ... pEZYmyc-HIS (Addgene, #18701) or pDEST-myc ...
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bioRxiv - Cancer Biology 2022Quote: ... ITGA2-HIS (Addgene, #51910), ITGB2-YFP (Addgene ...
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bioRxiv - Neuroscience 2024Quote: ... his-hOPTN (Addgene, #23053), mOPTN (mouse tissue cDNA) ...
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bioRxiv - Cell Biology 2024Quote: ... Eps15-GFP-His (Addgene #170860 ...
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bioRxiv - Molecular Biology 2020Quote: Insertion of the MS2 aptamer into different positions of the rrnB operon encoded in the pAM552 plasmid (a gift from A. Mankin lab, as in Addgene #154131) was performed by inverse PCR using primers Y-MS2-F and Y-MS2-R (Table 1) ...
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bioRxiv - Cell Biology 2022Quote: CymR was constructed via PCR and Gibson Assembly from the following constructs: pCuo CA Rac1 CMV + cumate operon purchased from Addgene (#84643), human APC open reading frame purchased from Addgene (#16507) ...
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bioRxiv - Synthetic Biology 2021Quote: A fresh single colony of E.coli EcM1 bearing pORTMAGE3 (Addgene Plasmid ID #72678) taken from LBa,Km was inoculated into 2 mL LBKm and incubated overnight at 30 °C ...
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bioRxiv - Biophysics 2023Quote: ... BL21(DE3) E.coli were transformed with the bacterial expression plasmid pET21a-αSynuclein (Addgene) containing the human αSyn coding sequence ...
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bioRxiv - Systems Biology 2021Quote: ... The pMEL16 (His-, Addgene 107922) and p414-TEF1p-Cas9-CYC1t (hereafter referred to as P414 ...
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bioRxiv - Biochemistry 2020Quote: ... insoluble His-tag purification (Addgene ID ...
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bioRxiv - Biochemistry 2020Quote: ... soluble His-tag purification (Addgene ID ...
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bioRxiv - Bioengineering 2023Quote: HIS-tagged HER2 (Addgene #16257) was expressed using the Expi293™ expression system (Thermo Fisher ...
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bioRxiv - Developmental Biology 2022Quote: E.coli strain BL21-DE3 was transformed with plasmid DNA pAG-MNase-6xHis (Addgene, plasmid #123461). Recombinant pAG-MNase was purified from cells grown in LB medium to OD600 0.6 at 37°C ...
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bioRxiv - Cancer Biology 2020Quote: ... pcDNA3.1-Flag-His-ATM (Addgene 31985), pcDNA3.1-mCherry-NLRP3 ...
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bioRxiv - Molecular Biology 2021Quote: ... and SERPINE1-bio-his (Addgene #52077) which were gifts from Gavin Wright without signal sequence to prevent cleavage of fused proteins (75) ...
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bioRxiv - Biochemistry 2020Quote: ... His-tag purification (Addgene ID: 139113)
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bioRxiv - Developmental Biology 2021Quote: pET-28b-RfxCas13d-His (Addgene #141322) plasmid containing the T7 promoter was linearized by using NotI restriction site ...
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bioRxiv - Developmental Biology 2023Quote: ... pcDNA3.0-BMAL1-His (AddGene CN: 31367), pcDNA3.0-3XFLAG-Cry2 plasmids and PEI max 40k (Polysciences Inc CN ...
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bioRxiv - Developmental Biology 2023Quote: pcDNA3-BMAL1-His (AddGene CN: 31367) were purchased from the Addgene ...
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bioRxiv - Immunology 2019Quote: ... and 6-His tag (Addgene plasmid# 50803) [36] ...