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Citations for Addgene :
1 - 50 of 103 citations for Inactive Serine Protease 35 PRSS35 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2023Quote: FLAG-IKK2 kinase inactive K44M (IKK2DN, Addgene, #15466) was cloned into pLenti-CMV-Puro DEST vector (Addgene ...
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bioRxiv - Molecular Biology 2021Quote: ... The expression vectors of the full-length SARS-CoV-2 spike and the human serine protease TMPRSS2 with a C-terminal C9-tag (TETSQVAPA) were acquired from AddGene (Summit Pharmaceutical International ...
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bioRxiv - Biochemistry 2022Quote: ... WT and inactive human TRPA1 variants were subcloned into CaM/pIRES2-eGFP (Addgene) at the NheI/EcoRI sites to generate a positive fluorescent readout for transfection in singly transfected calcium imaging studies ...
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bioRxiv - Developmental Biology 2021Quote: ... and TET1 catalytic inactive mutant (TET1CDmut) were amplified from FH-TET1-pEF (Addgene #49792) or pIRES-hrGFP II-mTET1 (Addgene #83569) ...
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bioRxiv - Neuroscience 2024Quote: ... The Opto-PLD active and inactive constructs were purchased from Addgene (140114, and 140061). The hPLD1 cDNA clone was a kind gift from Dr.
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bioRxiv - Immunology 2021Quote: ... NG-ABEmax (35) (Addgene plasmid #112095 ...
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Rab35 Governs Apicobasal Polarity Through Regulation of Actin Dynamics During Sprouting AngiogenesisbioRxiv - Developmental Biology 2022Quote: The following constructs were procured for this study: GFP-Rab35 S22N inactive (gift from Peter McPherson; Addgene plasmid # 47426); GFP-Rab35 WT (gift from Peter McPherson ...
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bioRxiv - Molecular Biology 2019Quote: The ATP sensor AT1.03 and its inactive variant AT1.03R122KR126K in the yeast expression vector pDRF1-GW were a gift from Wolf Frommer (Addgene plasmids #28003 and #28005 ...
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bioRxiv - Biochemistry 2023Quote: Catalytically inactive Mpro C145A mutants used for protein crystallography experiments were prepared by subcloning Mpro C145A from Addgene (catalog #141371) into a pET28a expression vector containing an N-terminal His-tag and tobacco etch virus (TEV ...
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bioRxiv - Molecular Biology 2022Quote: ... and AncBE4max (Addgene #112100, (35)) were amplified using primer 1006F and 1007R and cloned into SpeI and FseI sites of pTB007 (5) ...
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bioRxiv - Cell Biology 2023Quote: ... AICSDP-35:AAVS1-mEGFP (Addgene plasmid #91565 ...
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bioRxiv - Plant Biology 2024Quote: ... and CaMV 35S terminator (Addgene #50337) were used in all the Cas protein expression cassettes ...
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bioRxiv - Cell Biology 2024Quote: ... 35 and Ubc9 (Addgene plasmid # 20082) 36 was a gift from Dr ...
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bioRxiv - Plant Biology 2024Quote: ... and pICH51288 and pICH41414 (2×35S and 35S terminator; Addgene #50269 and #50337; Engler et al., 2014) in a BsaI Golden Gate reaction to generate binary vectors containing the fragment-swapped Rcr3/Pip1 hybrids driven by the double 35S CaMV promoter and targeted to the apoplast using a NtPR1a signal peptide ...
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bioRxiv - Plant Biology 2021Quote: ... and 35S terminator (Addgene, catalog number: 50337) in the level 1 vector pICH47802 (Addgene ...
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bioRxiv - Plant Biology 2021Quote: ... and pICH41414 (35S terminator, Addgene no. 50337) modules ...
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bioRxiv - Plant Biology 2021Quote: ... and pICH41414 (35S terminator, Addgene no. 50337) into the binary vector pICH47732 (Addgene no ...
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bioRxiv - Biophysics 2021Quote: Plasmids for the expression of wildtype SpCas9 and the catalytically inactive SpCas9 variants carrying the mutations D10A/H840A were obtained from Addgene (plasmid ID: 39312 and 39318). SpCas9 variants that carried a single mutation of the catalytic site (D10A or H840A ...
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bioRxiv - Genomics 2021Quote: ... KRAB and MeCP2 were linked to dCas9 with flexible glycine-serine linkers and cloned into lentiCas9-Blast (Addgene 52962) (22).
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bioRxiv - Plant Biology 2023Quote: ... the AtCas9 (2×35S::AtCAS9-OCST; Addgene #112079) and the linker pICH41780 (Addgene #48019 ...
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bioRxiv - Molecular Biology 2022Quote: ... newly constructed vectors and pEGIP*35 (Addgene, Cat #26776) were packaged into lentivirus individually ...
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bioRxiv - Synthetic Biology 2024Quote: ... The plasmid backbone pAM552G (35, 47) (Addgene accession #154131) was used for testing ribosome variants from RISE in vivo (see below) ...
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bioRxiv - Synthetic Biology 2021Quote: ... 35 into lenti sgRNA(MS2)_puro backbone (Addgene plasmid #73795); then U6-gRNA-miniCMV-TdTomato was inserted into pCR Blunt II-TOPO ...
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bioRxiv - Biochemistry 2024Quote: ... and Peredox-mCherry (#32380) 35 constructs were obtained from AddGene. Enolase-RFP ...
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bioRxiv - Biochemistry 2023Quote: ... Ube1 was a gift from Cynthia Wolberger 35(Addgene plasmid # 34965).
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bioRxiv - Cell Biology 2023Quote: ... AICSDP-35:AAVS1-mEGFP (Addgene plasmid #91565; http://n2t.net/addgene:91565; RRID:Addgene_91565), AICSDP-42:AAVS1-mTagRFPT-CAAX (Addgene plasmid #107580 ...
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bioRxiv - Developmental Biology 2023Quote: ... sfGFP sequence was amplified from the 35S-sfGFP-nosT plasmid135 (Addgene # 80129) using primers UsfGM-F1 and UsfGnes-R1 ...
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bioRxiv - Synthetic Biology 2023Quote: ... TEV protease (Addgene, pRK793). Makrolon 2808 (gift from Covestro).
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bioRxiv - Neuroscience 2024Quote: ... and pAAV-PTRE-tight-hM3Dg-mCherry(35) (a gift from William Wisden [Addgene plasmid # 66795 ...
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bioRxiv - Genetics 2020Quote: ... the NLS:LEXA (1-214aa) fragment from the pBPnlsLexA::GADflUw plasmid [35] (Addgene plasmid #26232) was cloned in frame in a myc-BAP170 (2-1688 aa ...
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bioRxiv - Genetics 2023Quote: ... The pFA6a-3HA-KanMX and pCY3090-07 plasmids were obtained from Addgene (35, 39). The pBSS2-SAT1 flipper plasmid was provided to us by P ...
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bioRxiv - Molecular Biology 2021Quote: ... et al.35 The pL-CRISPR-SFFV-tRFP plasmid was obtained from Addgene (Plasmid #57826) and originally deposited by the Ebert lab.34
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bioRxiv - Plant Biology 2019Quote: ... placing fluorescent protein expression under the control of the cauliflower mosaic virus 35S promoter (plasmids pN_35S/mEGFP and pN_35S/mApple available at www.addgene.org as plasmids #132565 (RRID:Addgene_132565) and #132566 (RRID:Addgene_132566) ...
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bioRxiv - Biochemistry 2022Quote: ... Yi Zhang (Addgene plasmids # 60941 and 60942, http://n2t.net/addgene:60941, RRID:Addgene_60941, and http://n2t.net/addgene:60942, RRID:Addgene_60942 [35]). The DNA oligonucleotides used in this work are summarized in Table 1 ...
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bioRxiv - Neuroscience 2020Quote: ... After dialysis SenP1 protease (Addgene plasmid #16356) [57] was added and the enzymatic cleavage was performed for 4 hours at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... The open reading frames of the HAs of A/Vietnam/1203/2004 H5 (Addgene plasmid #182546, [35]), A/Puerto-Rico/8/1934 H1 [39] ...
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bioRxiv - Cancer Biology 2022Quote: Single guide RNA sequences targeting murine Lrp1 were obtained from the GeCKO v2 library (35) and cloned into the pSpCas(BB)-2A-Puro (PX459) V2.0 vector (RRID:Addgene_62988). B16F10-TR-shApoe cells were plated into a 6-well dish the day prior to transfection and transfected with 4 μg plasmid and TurboFect transfection reagent (Thermo Scientific ...
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bioRxiv - Plant Biology 2024Quote: 35S:GUS-pMAS:GFP was modified by replacing the 35S promoter with the MAS promoter from pGFPGUSPlus (Plasmid #64401, Addgene) (He et al. ...
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bioRxiv - Neuroscience 2024Quote: ... and pAAV-PTRE-tight-hM3Dg-mCherry(35) (a gift from William Wisden [Addgene plasmid # 66795; http://n2t.net/addgene:66795; RRID:Addgene_66795]). Specifically ...
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bioRxiv - Cell Biology 2022Quote: ... His14-Tev-tagged SENPEuB protease (Addgene ID #149333) was expressed in E ...
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bioRxiv - Cell Biology 2019Quote: Human GeCKOv2 CRISPR knockout pooled library (Pooled Library #1000000048)35 and pX330-U6-Chimeric_BB-CBh-hSpCas9 (Addgene plasmid # 42230)97 were gifts from Feng Zhang ...
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bioRxiv - Synthetic Biology 2021Quote: The pEditor fragment of AID_T7_UGI was amplified from the vector pcDNA3.1 (+)- Hyperactive AID-T7 RNA Polymerase-UGI-T2A-td Tamato (Addgene 138610; [35]) using primers SphI_AID_T7_F and NSiI_XbaI_AID_T7_R ...
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bioRxiv - Plant Biology 2023Quote: ... and rbcS-E9 enhancers and their 169-bp 3′ and 5′ segments as well as the 35S enhancer were PCR amplified from pZS*11_4enh (Addgene no ...
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bioRxiv - Cell Biology 2023Quote: ... wash buffer containing 500 nM SUMOEu1 protease (Addgene #149333) was added to the beads and left to incubate for 30 minutes with mixing at 4°C ...
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bioRxiv - Developmental Biology 2023Quote: ... tobacco vein mottling virus protease (TVMVp; Addgene Plasmid #8832), and human rhinovirus 3C protease (HRV 3Cp ...
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bioRxiv - Bioengineering 2024Quote: ... pET28-MBP-super TEV protease (Addgene plasmid ID 171782) (Keeble et al. ...
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bioRxiv - Plant Biology 2020Quote: The human codon-optimized CAS9 with 2×35S CaMV promoter and Nos terminator was amplified from pAGM4723 plasmid (Addgene# 49772) using KpnI forward and PacI reverse primers (Supplemental Table 9) ...
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bioRxiv - Microbiology 2020Quote: ... The sfgfp gene was PCR-amplified with primers 35 and 36 from plasmid pHR-scFv-GCN4-sfGFP-GB1-NLS-dwPRE (gift from Ron Vale; Addgene plasmid # 60906 ...
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bioRxiv - Plant Biology 2023Quote: ... The module for selection was obtained from pHDE-35S-Cas9-mCherry-UBQ which was a gift from Yunde Zhao (Addgene plasmid # 78932 ...
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bioRxiv - Cell Biology 2023Quote: ... 2-6×105 cells in a 35 mM well were transfected with 0.4ug of DNA encoding a puro resistance plasmid (pCoPURO, Addgene #17533) using Effectene Transfection Reagent (Qiagen) ...