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Citations for Addgene :
1 - 50 of 1064 citations for Human Glutaminyl tRNA Synthetase QARS Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: ... and ANAP-specific amber suppressor tRNA/tRNA synthetase pair (Addgene #48696 (Chatterjee and Guo, 2013)) ...
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bioRxiv - Physiology 2023Quote: ... the pAS_4xG1 PylT FLAG-G1 PylRS Y125A tRNA/aminoacyl-tRNA synthetase plasmid (Addgene, Watertown, MA #154773) was used ...
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bioRxiv - Biophysics 2024Quote: ... FLAG-Mma PyIRS (Mma PylT/RS) and pAS_4xhybPylT A41AA C55A FLAG-G1 PylRS Y125A (G1PylT/RS) tRNA/aminoacyl-tRNA synthetase pairs from Addgene (Watertown ...
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bioRxiv - Physiology 2023Quote: ... FLAG-Mma PyIRS (Mma PylT/RS) and pAS_4xhybPylT A41AA C55A FLAG-G1 PylRS Y125A (G1PylT/RS) tRNA/aminoacyl-tRNA synthetase pairs from Addgene (Watertown ...
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bioRxiv - Cell Biology 2019Quote: ... The pDB070.iodo.5 plasmid containing the modified tRNA and tRNA synthetase for p-iodo-L-phenylalanine incorporation was obtained from Addgene (#99397) as a gift from David Liu [31] ...
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bioRxiv - Biochemistry 2022Quote: ... cells with an additional plasmid encoding for an arabinose-inducible promoter to express Amber-suppressor tRNA (tRNACUA) and a biorthogonal tRNA synthetase (Addgene #31186)(37) ...
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bioRxiv - Biophysics 2022Quote: The cDNA sequence of the aminoacyl-tRNA synthetase was obtained from the plasmid pANAP (Addgene #48696) (Chatterjee and Guo ...
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bioRxiv - Neuroscience 2024Quote: The construct utilized for a transfection control comprised of an overexpression of methionine-tRNA synthetase (MeTRS) (AddGene, pMarsL274G) were created and sequenced using protocol described above.
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bioRxiv - Biochemistry 2023Quote: ... Bpa-mutant Pet28n-6xHis-BRCA11-104-(GS)6-BARD126-221 plasmids were co- transformed with a pEVOL-pBpF plasmid encoding an Bpa aminoacyl tRNA synthetase (gift from P. Schultz, Addgene #31190) in E ...
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bioRxiv - Biochemistry 2023Quote: ... Approximately 3ug of AzF tRNA/RS DNA (AddGene Plasmid #105829) and 3ug of PFKL (tagged with the FLAG epitope ...
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bioRxiv - Physiology 2019Quote: HepG2 cells were transfected with the luciferase expression construct under the Fatty Acid Synthetase (FAS) Promoter (Addgene# 8890) along with β-Gal plasmid (Ambion Cat ...
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bioRxiv - Biophysics 2022Quote: The amber suppressor tRNA sequence was translated from the plasmid pANAP (Addgene #48696) (Chatterjee and Guo ...
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bioRxiv - Cell Biology 2021Quote: The tRNA expression vector was a kind gift from Simon Elsässer (Addgene number: 140008). For cloning the BRET extracellular sensors ...
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bioRxiv - Cancer Biology 2020Quote: pCFDg1-5 gRNA-tRNA array was constructed stepwise as previously described using pCFD5 (Addgene #73914)11 as a template and V8 targeting gRNAs.
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bioRxiv - Molecular Biology 2022Quote: ... Brunello library targeting all human protein-coding genes28 was purchased from Addgene (#73179). Quality of the MYC-CRISPR and Brunello libraries was verified by next-generation sequencing on Illumina platform (BGI ...
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bioRxiv - Physiology 2022Quote: ... a DNA block containing sgEGFP-tRNA-Hnf4a-sg2 was PCR-amplified using pGTR plasmid (Addgene #63143) as a template and primers listed in Table S2 (see also Fig ...
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bioRxiv - Cell Biology 2022Quote: ... cDNAs of the human proteins were cloned into pTT5 based expression vectors (Addgene #52355). The constructs were tagged with Twin-Strep-tag (SII ...
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bioRxiv - Microbiology 2023Quote: ... plasmid containing human codon-optimized Streptococcus pyogenes Cas9 protein and Stk3 (Mst2, Addgene #75975) gRNA was used to dually target Mst1 and Mst2 ...
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bioRxiv - Cell Biology 2021Quote: ... The cells were transfected with 0.45 μg of the indicated constructs and 0.05 µg of tRNA/synthetase50 (Addgene number ...
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bioRxiv - Biophysics 2019Quote: The cDNAs for protein expression in this study were as follows: human Tau-2N4R (Addgene #16316), human MAP7 (GE Dharmacon MGC Collection #BC025777) ...
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bioRxiv - Cell Biology 2021Quote: ... Corresponding oligonucleotides were annealed and cloned in the pX330 plasmid expressing human SpCas9 protein (Addgene #42230). The donor plasmids were constructed as follows ...
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bioRxiv - Genetics 2023Quote: ... This was done by appending guide RNA sequences to tracrRNA core and tRNA sequences from pMGC (Addgene, Plasmid #112812) through tailed primers (Table S3 ...
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bioRxiv - Cell Biology 2021Quote: ... hLamp1-BFP (#1016) plasmid encoding BFP-labeled version of human Lamp1 protein was from Addgene (Cat# 98828). GFP-hRab14.dn3 (#1017) ...
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bioRxiv - Immunology 2021Quote: ... Plasmid expressing the human ACE2 protein with a C-terminal C9 tag was obtained from Addgene (Plasmid 1786). 293T cells were transduced with retroviral particles carrying a pQCXIP vector encoding the gene for the human ACE2 protein ...
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bioRxiv - Cell Biology 2021Quote: ... The mCh-hRab7A (#922) plasmid encoding mCherry-labeled version of human Rab7A protein was from Addgene (Cat# 61804). GFP-hRab5A.dn3 (#966) ...
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bioRxiv - Cell Biology 2021Quote: The RFP-hRab5A.dn3 (#921) plasmid encoding RFP-labeled version of human Rab5A protein was from Addgene (Cat# 14437). The mCh-hRab7A (#922 ...
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bioRxiv - Cell Biology 2024Quote: ... We next used the human EZH2 primary protein sequence available in the addgene for hEZH2 plasmid (#24230, Addgene) and predicted the possible cysteine residues using the GPS-SNO prediction tools ...
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bioRxiv - Plant Biology 2020Quote: ... and containing a sgRNA backbone as well as an alanine tRNA spacer (see S3 File for an annotated sequence listing; the plasmid has been deposited with Addgene, TBA); and pCBC-DT1DT2 (Xing & al. ...
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bioRxiv - Cell Biology 2021Quote: ... The plasmid for the expression of the tRNA/aminoacyl transferase pair (pNEU-hMbPylRS-4xU6M15, herein termed PylRS/4xtRNAPyl) was a gift from Irene Coin (Addgene, #105830)44 ...
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bioRxiv - Biophysics 2022Quote: ... with the mutations Y271A and Y349F and the improved pyrrolysyl-tRNA M15 was generated based on the plasmid pNEU-hMbPylRS-4xU6M15 [33] (Addgene plasmid #105830). To reduce nuclear labeling [34] ...
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bioRxiv - Neuroscience 2022Quote: ... a mammalian expression vector encoding an EGFP-human 0N4R tau fluorescent fusion protein was obtained from Addgene (catalog # 46904). This plasmid was developed by the lab of Karen Ashe [16] ...
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bioRxiv - Genetics 2024Quote: Human pcDNA3-FLAG-RBBP5 plasmid used in the protein expression experiments was obtained from Addgene (Cat# 15550, MA, USA). Candidate variants were introduced using QuikChange II Site-directed mutagenesis kit according to manufacturer’s protocol (Agilent ...
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bioRxiv - Cancer Biology 2022Quote: ... human E2F5 and human DP1 were purchased from Addgene, whereas the ORF encoding human p130 from Origene.
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bioRxiv - Cancer Biology 2021Quote: Cas9-expressing stable cell lines were produced by infection with the lentivirus encoding human codon-optimized Streptococcus pyogenes Cas9 protein (LentiV_Cas9_puro, Addgene plasmid # 108100) and subsequent selection with 1 μg/mL puromycin ...
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bioRxiv - Biochemistry 2022Quote: ... was made by ligating a PCR fragment encoding full-length human protein between ApaI and BamHI sites of pHis10-PS-SNAPf (75) (gift from Ron Vale; Addgene plasmid #78512 ...
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bioRxiv - Biophysics 2019Quote: Expression of N-terminally acetylated human αS and βS proteins was performed via co-expression with pNatB plasmid (Addgene #53613) in E ...
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bioRxiv - Cell Biology 2021Quote: ... and GFP-hRab31.dn3 (#1019) plasmids encoding GFP-labeled versions of the indicated human wild-type proteins were from Addgene (Cat# 49549 ...
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bioRxiv - Neuroscience 2023Quote: ... An AAV8 encoding the inhibitory designer receptor KORD fused to the fluorescent protein mCitrine under the control of the human synapsin promoter in a Cre-dependent manner was obtained from Addgene (AAV8-hSyn-dF-HA-KORD-IRES-mCitrine ...
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bioRxiv - Neuroscience 2023Quote: ... encoding the Cre enzyme fused to the green fluorescent protein (GFP) under the control of the human synapsin promoter was obtained from Addgene (AAVrg.hSyn.HI.eGFP-Cre.WPRE.SV40 ...
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bioRxiv - Neuroscience 2022Quote: ... 250nl of an anterograde adeno-associated virus (AAV) vector expressing a green fluorescent protein under the hSyn (human synapsin) promoter (AAV5-hSyn-eGFP; Addgene) was injected into ACC to fluorescently mark the anatomical boundary of the claustrum (White et al. ...
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bioRxiv - Cell Biology 2020Quote: ... human KLF4 (RRID:Addgene_17219), human c-MYC (RRID:Addgene_17220 ...
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bioRxiv - Cell Biology 2020Quote: ... human SOX2 (RRID:Addgene_17218), human KLF4 (RRID:Addgene_17219) ...
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bioRxiv - Neuroscience 2022Quote: ... human DNAJB4 (Addgene) and DNAJB6 (Addgene ...
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bioRxiv - Microbiology 2020Quote: ... Vesicular stomatitis virus G protein (VSV-G) pseudotyped lentiviruses expressing human ACE2 were produced by transient co-transfection of pMD2G (Addgene #12259) and psPAX2 (Addgene #12260 ...
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bioRxiv - Cell Biology 2020Quote: ... was made by amplifying TPD54 by PCR from human Tumor protein D54 (IMAGE clone: 3446037) and inserting into pIRES-EGFP-puro (Addgene #45567) via NheI and XhoI ...
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bioRxiv - Microbiology 2022Quote: To make human ACE2 protein, pcDNA3-sACE2-WT(732)-IgG1 (Chan et al., 2020) (Addgene plasmid #154104, gift of Erik Procko) plasmid was transfected into Expi293 cells using PEI at a ratio of 1:3 ...
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bioRxiv - Biochemistry 2023Quote: ... Human ATAD2B bromodomain-containing protein (residues 953−1085, Uniprot code: Q9ULI0) was a gift from Nicole Burgess-Brown (Addgene plasmid # 39046) was PCR-amplified and cloned into pDEST15 (GlaxoSmithKline ...
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bioRxiv - Systems Biology 2022Quote: ... The nuclear marker H2B-miRFP703 is a fusion of the human H2B clustered histone 11 (H2BC11) with the monomeric near-infrared fluorescent protein miRFP703 (Shcherbakova et al. 2016) (Addgene plasmid #80001). ERK-KTR-mRuby2 ...
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bioRxiv - Cell Biology 2023Quote: ... they were electroporated with Yamanaka’s plasmids (plasmid numbers 27078 (human SOX2 and KLF4), 27080 (human L-MYC, LIN28), 27077 (human OCT3/4, shRNA against TP53) from Addgene, www.addgene.org ...
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bioRxiv - Biophysics 2019Quote: ... human DCX (Addgene #83928), mouse DCLK1 (Transomics #BC133685) ...