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Citations for Addgene :
1 - 50 of 1757 citations for Heat Shock Factor Protein 1 HSF1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2019Quote: ... (Addgene #60948)69 or pBS130 (encoding phiC31 integrase under control of a heat shock promoter, Addgene #26290)70 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Site-directed integration into attP sites was achieved by co-injection of an attB-containing vector (400 ng µl-1) and pBS130 (encoding phiC31 integrase under control of a heat shock promoter (Addgene #26290) [73]) ...
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bioRxiv - Neuroscience 2023Quote: ... Site-directed integration into attP sites was achieved by co-injection of an attB-containing vector (400 ng µl-1) and pBS130 (encoding ΦC31 integrase under control of a heat shock promoter (Addgene #26290)60) ...
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bioRxiv - Bioengineering 2023Quote: ... MCP-p65-HSF1 (Addgene #61423), scFv-VP64 (Addgene #60904) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... coli competent cells were transformed by heat shock with a vector pETM33_Nsp5_Mpro (a gift from Ylva Ivarsson; Addgene plasmid # 156475). Bacteria were cultured on LB medium with kanamycin [50 ug/ml] ...
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bioRxiv - Physiology 2020Quote: ... and MS2-P65-HSF1 (Addgene: 61423) were sub-cloned into pAdv5 vector (Invitrogen ...
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bioRxiv - Developmental Biology 2022Quote: ... and MS2-p65-HSF1 (from Addgene 61423) fragments were inserted to generate a doxycycline (DOX ...
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bioRxiv - Cell Biology 2022Quote: ... and MS2-P65-HSF1 constructs (Addgene #89308). For the genetic screening ...
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bioRxiv - Genetics 2021Quote: ... and MS2-P65-HSF1-Hygro (Addgene 61426) were established by transduction of corresponding lentivirus following selection with 10 μg/mL Blasticidin (Invivogene ...
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bioRxiv - Bioengineering 2023Quote: ... the MCP-p65-HSF1 plasmid (Addgene #61423) was digested with BamHI and NheI and respective effector domains were cloned using NEBuilder HiFi DNA Assembly ...
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bioRxiv - Developmental Biology 2020Quote: ... and lenti MS2-p65-HSF1-Hygro (Addgene 61426) into TS-Rs26 mTSCs ...
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bioRxiv - Genomics 2020Quote: ... with lenti-MS2-p65-HSF1-Hygro (Addgene #61426) were used to generated cells stably expressing the knockout or gene activation machinery ...
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bioRxiv - Molecular Biology 2022Quote: ... and MS2-P65-HSF1 activator plasmid (Addgene #61426) at a molar ratio of 2.5:1:10 ...
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bioRxiv - Genomics 2023Quote: ... and lenti-MS2-p65-HSF1-Hygro (Addgene #89308) were used to generate stable cell lines for gene knockout and activation ...
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bioRxiv - Cancer Biology 2023Quote: ... and lenti-MS2-p65-HSF1-Hygro (Addgene #89308) were used to generate stable cell lines for gene activation ...
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bioRxiv - Synthetic Biology 2023Quote: ... p65-HSF1 was cloned from plasmid #61423 (Addgene), and KRAB-MeCP2 was cloned from plasmid #167902 (Addgene) ...
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bioRxiv - Developmental Biology 2023Quote: ... and MS2-P65-HSF1 were purchased from Addgene (Addgene ...
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bioRxiv - Developmental Biology 2024Quote: ... and MS2-P65-HSF1) were purchased from Addgene (Addgene ...
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bioRxiv - Molecular Biology 2023Quote: ... dCas9-VP64 and MCP-p65-HSF1 (gift from Feng Zhang, Addgene #61425 and #61426), were infected with the lentiviral SAM sgRNA library with a low titer of MOI=0.5 ...
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bioRxiv - Cell Biology 2023Quote: ... dCas9-VP64 and MCP-p65-HSF1 (kind gift from Feng Zhang, Addgene #61425 and 61426), were infected with the lentiviral SAM library at a low viral titer (MOI=0.5 ...
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bioRxiv - Cell Biology 2023Quote: ... Both GFP11-tagged Fluc proteins and GEM transcriptional factor (cloned from pJW1663, Addgene plasmid #112037) were stably integrated into yeast genome ...
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bioRxiv - Synthetic Biology 2021Quote: ... and EF1α-scFv-p65-HSF1-T2A-EGFP-WPRE-PolyA (Addgene plasmid #107311, a gift from Hui Yang)12 and inserted into lentiMPH v2 and lenti dCAS-VP64_Blast backbone respectively ...
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bioRxiv - Molecular Biology 2019Quote: ... dCas9-expressing cells were then infected with the MS2-P65-HSF1 activator helper complex (Addgene, 61426-LVC) and treated with 0.5 mg/ml hygromycin ...
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bioRxiv - Cancer Biology 2022Quote: ... SK-N-DZ cells expressing dCas9-VP64 were transduced with lentiviral particles encoding the p65-HSF1 transactivator complex (pLentiMPH2, Addgene plasmid #89308 was a gift from Feng Zhang) ...
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bioRxiv - Bioengineering 2023Quote: ... 1x 9aa TADs were designed and annealed as double strand oligos and then cloned into the BamHI/NheI-digested MCP-p65-HSF1 backbone plasmid (Addgene #61423) using T4 ligase (NEB) ...
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bioRxiv - Bioengineering 2023Quote: ... Selected fusions between 3x 9aa TADs and eNRF2 were generated using gBlock (IDT) fragments and cloned into the BamHI/NheI-digested MCP-p65-HSF1 backbone plasmid (Addgene #61423) using NEBuilder HiFi DNA Assembly ...
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bioRxiv - Genetics 2020Quote: ... Plasmids generated for this work for heat shock Cas9 expression (pJJF152) and proof of concept sgRNAs (targeting SECGFP, dpy-10, sqt-3) will be available from Addgene as indicated in a supplementary table or for specific sgRNAs upon direct request from the authors.
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bioRxiv - Genomics 2020Quote: ... The LentiSAMPHv2 plasmid was assembled by adding the P2A-MS2-p65-HSF1 fragment amplified from the lentiMPH v2 plasmid (Addgene plasmid #89308) (32 ...
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bioRxiv - Cancer Biology 2020Quote: ... The resulting HeLa-dCas9 cells were then transduced with the lentiviral construct for the MS2-P65-HSF1 (MPH) activator complex (Addgene 61426-LVC) and selected with 0.5 mg/ml hygromycin ...
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bioRxiv - Bioengineering 2022Quote: ... The T-cell factor/lymphoid enhancer factor (TCF/LEF) luciferase reporter SuperTopFlash (STF) and the control pRL-SV40 Renilla luciferase constructs (Addgene) were used for Wnt/β-catenin-responsive reporter assays ...
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Comprehensive fitness landscape of SARS-CoV-2 Mpro reveals insights into viral resistance mechanismsbioRxiv - Molecular Biology 2022Quote: ... The LexA_ER_B112 transcription factor was amplified from Addgene_58437 (FRP880_PACT1(−1-520)-LexA-ER-haB112-TCYC1 was a gift from Joerg Stelling ...
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bioRxiv - Molecular Biology 2020Quote: ... At least two independent sets of oligonucleotide pairs for gene knockdown of human MTs and the transcription factor MTF-1 (supplementary table S4) were synthesized and cloned into the pLKO.1-TCR vector (Addgene, Cambridge, MA, USA); the same vector was also used as an empty vector control ...
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bioRxiv - Cell Biology 2022Quote: A His-tagged mouse Capping Protein (F-actin-capping protein subunit alpha-1 and F-actin-capping protein subunit beta) plasmid was obtained from Addgene as described above (Plasmid #89950) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... A human elongation factor 1 (EF1) promoter was introduced using a Gateway att4-att1 Entry clone (Addgene, Watertown, MA, #162920). Final expression clones were verified by restriction analysis and maxiprep DNA was prepared using the Qiaprep Maxiprep kit (Qiagen ...
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bioRxiv - Molecular Biology 2024Quote: ... reporter cell lines were generated by TALEN-mediated homology-directed repair to integrate enhancer reporter donor constructs into the AAVS1 locus by electroporation of 1 × 106 K562 cells (or K562 cells selected to have the desired synthetic transcription factor) with 1 ng of reporter donor plasmid and 0.5 ng of each TALEN-L (Addgene no. 35431) and TALEN-R (Addgene no ...
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bioRxiv - Cell Biology 2023Quote: ... fibroblasts were reprogrammed using lentiviral vectors that carried six transcription factors (ADDGENE/PSIN4-EF2-N2L ...
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bioRxiv - Neuroscience 2024Quote: The transcription factors and the reverse tetracycline transactivator (rtTA) were obtained from Addgene (Tet-O-Fuw-Ascl1 Addgene plasmid #27150 ...
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bioRxiv - Cell Biology 2023Quote: ... Ras GTPase-activating protein-binding protein 1 (G3BP1) phage UbiC G3BP1-GFP-GFP was a gift from Jeffrey Chao (Addgene plasmid # 119950 ...
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bioRxiv - Immunology 2023Quote: Pseudovirus expressing Wuhan-Hu-1 SARS-CoV-2 S protein were produced by co-transfection of plasmids encoding a GFP protein (Addgene, 11619), a lentivirus backbone (VRC5602 ...
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bioRxiv - Genomics 2020Quote: pNTI729 was constructed by first amplifying the ZEM transcription factor from pNTI638 (pHES795 Addgene #87943) (17 ...
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bioRxiv - Neuroscience 2021Quote: ... and the Egr1 transcription factor (pcDNA3-Egr1, a gift from Eileen Adamson, Addgene plasmid #11729) known to stimulate the Cav3.2 promotor 25.
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bioRxiv - Cell Biology 2023Quote: ... fibroblasts were reprogrammed using lentiviral vectors that carried six transcription factors (ADDGENE/PSIN4-EF2-N2L, ADDGENE/PSIN4-EF2-O2S and ADDGENE/PSIN4-CMV-K2M) ...
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Upregulated GIRK2 counteracts ethanol-induced changes in excitability & respiration in human neuronsbioRxiv - Neuroscience 2024Quote: ... NPCs were induced into neurons with doxycycline-inducible transcription factor NGN2 with neomycin antibiotic selection (Addgene #99378), following the protocol described by (Ho et al. ...
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bioRxiv - Immunology 2020Quote: ... and the full-length protein (IFT20, aa 1-132) in-frame with the tags into the pEGFP-N1 (#6085-1 Addgene) and pGEX-6P-2 vectors (#27-4598-01Addgene) ...
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bioRxiv - Neuroscience 2022Quote: ... the complementary DNA (cDNA) for each iMN factor (Ngn2, Lhx3, Isl1, NeuroD1, Ascl1, Brn2 and Myt1l) was purchased from Addgene and cloned into the pMXs retroviral expression vector using Gateway cloning technology (Invitrogen) ...
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bioRxiv - Developmental Biology 2022Quote: ... Samples were incubated with Protein A/G-MNase fusion protein (Addgene, Cat #123461) at 700 ng/mL for 1 hour at 4°C ...
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bioRxiv - Immunology 2021Quote: ... green fluorescence protein (GFP) or an enhanced yellow fluorescent protein (EYFP) sequence (pcDNA1.3-, Addgene) using standard cloning methods ...
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bioRxiv - Genetics 2019Quote: ... mCherry fluorescent protein marker (Addgene), and ampicillin resistance gene ...
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bioRxiv - Synthetic Biology 2024Quote: ... Genes encoding natural transcription factors were sourced from: cJun (pCLXSN-c-JUN, which was a gift from Jin Chen, Addgene plasmid #102758)36 and BATF (pFUW-TetO-BATF ...
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bioRxiv - Cell Biology 2020Quote: ... and WD repeat domain phosphoinositide-interacting protein 1 (WIPI1) cDNA was a gift from Noboru Mizushima (Addgene plasmid # 38272) (Itakura & Mizushima ...