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Citations for Addgene :
1 - 50 of 993 citations for Estrogen receptor ER alpha Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: ... A plasmid containing estrogen receptor alpha (pEGFP-C1-ERα) was obtained from Michael Mancini (Addgene #28230) and mutated into a constitutively active form (pEGFP-C1-ERαY537S)29 using the Q5® Site-Directed Mutagenesis Kit (New England Biolabs ...
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bioRxiv - Neuroscience 2024Quote: ... we cloned CreERT2 (Cre recombinase fused to a mutant ligand-binding domain of the estrogen receptor) and IRES into the BamHI site of pAAV-EF1a-tdTomato-WPRE-pGHpA (Addgene #67527). To prevent leakage of Cre activity34 ...
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bioRxiv - Biophysics 2019Quote: ... human α-actinin 1 cDNA (Addgene; pEGFP-N1 alpha-actin1) was truncated at the actin binding domain (Asp1-Ala247) ...
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bioRxiv - Cell Biology 2020Quote: ... and the ER localization marker mCherry-ER-3 (Addgene: 55041) for 2 days ...
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bioRxiv - Cell Biology 2023Quote: The ER-membrane targeting sequence from ER-ABKAR (Addgene #61508) was amplified by PCR ...
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bioRxiv - Cell Biology 2024Quote: ... The ER-Halo and the ER-GCaMP6-150 (Addgene #86918) under a CamKII promoter were both restriction digested with AgeI and the HALO protein was ligated with Quick Ligase (NEB ...
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bioRxiv - Bioengineering 2023Quote: ... ER (Addgene 37261), miniIAA7 (Addgene 129721) ...
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bioRxiv - Biochemistry 2020Quote: ... For fluorescence imaging of ER and Golgi pmTurquoise2-ER (Addgene #36204) and pmTurquoise2-Golgi (Addgene #36205 ...
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bioRxiv - Neuroscience 2021Quote: ... were infused bilaterally with AAV expressing the inhibitory designer receptor human M4 muscarinic receptor (hM4Di; AAV8-hSyn-hM4Di-mCherry, 4.8 × 1012 or 3.7 × 1012 vg/mL; Addgene; 0.3 μL) at a rate of 0.1 μL/min into the mOFC (AP ...
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bioRxiv - Neuroscience 2022Quote: ... Gqi5 and mouse A1 receptor or human D2 receptor were established by transfecting CHO cells with pCAG-cyto-RCaMP (Addgene), pME-Gqi5 (Yamashiro et al*** ...
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bioRxiv - Neuroscience 2023Quote: ... and AAV encoding the cre-inducible inhibitory designer receptor human M4 muscarinic receptor (hM4DGi; AAV2-Syn-DIO-hM4Di-mCherry; Addgene) or fluorophore control (AAV2-Syn-DIO-mCherry ...
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bioRxiv - Cancer Biology 2023Quote: The human Insulin Receptor cDNA was a gift from Frederick Stanley (Addgene plasmid # 24049 ...
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bioRxiv - Cell Biology 2022Quote: ... ER-pmTurquoise2 (Addgene #36204), Mitochondria-pmTurquoise2 (Addgene #36208) ...
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bioRxiv - Cell Biology 2022Quote: ER-mRFP (Addgene #62236), mTagRFP-T2-Mito-7 (Addgene #58041 ...
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bioRxiv - Cell Biology 2023Quote: ... ER-StayGold (Addgene #186296), mCherry-Drp1 (Addgene #49152) ...
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bioRxiv - Biochemistry 2024Quote: ER-mScarletl70 (Addgene 137805) was used as an ER organellar marker.
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bioRxiv - Neuroscience 2023Quote: ... and AAV encoding the cre-inducible excitatory designer receptor human M3 muscarinic receptor (hM3Dq; AAV2-Syn-DIO-hM3Dq-mCherry; Addgene, Watertown, MA) or fluorophore control (AAV2-Syn-DIO-mCherry ...
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bioRxiv - Cell Biology 2020Quote: mCherry-ER fusion protein was amplified by PCR from mCherry-ER-3 plasmid (Addgene) and cloned into Gateway modified pBABE (in house) ...
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bioRxiv - Microbiology 2020Quote: ... pMch-sec61-beta shows ER and the ER-Golgi intermediate compartment (Addgene cat 49155) (40) ...
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bioRxiv - Molecular Biology 2022Quote: ... optoPLD-ER (Addgene plasmid # 140060) and optoPLD-ER ‘dead’ (Addgene plasmid # 140059 ...
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bioRxiv - Neuroscience 2021Quote: ... ER-GCaMP6-150 (Addgene #86918) (20) ...
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bioRxiv - Cell Biology 2021Quote: ... mEmerald-ER-3 (Addgene plasmid #54082), and calnexin-mEmerlad (Addgene plasmid #54021 ...
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bioRxiv - Cell Biology 2019Quote: ... and mTurquoise2-ER (Addgene plasmid # 36204) was a gift from Dorus Gadella ...
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bioRxiv - Biochemistry 2023Quote: ... the plasmids pmTurquoise2-ER (Addgene #36204) and pmTurquoise2-Golgi (Addgene #36205 ...
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bioRxiv - Biochemistry 2023Quote: ... mCherry-ER-3 (Addgene, Watertown, MA), or Perilipin1-YFP[41] following established protocols[32] ...
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bioRxiv - Microbiology 2023Quote: ... Human codon-optimized full-length Eph receptors were subcloned from pDONR223-EphB1 (Addgene # 23930 (82)) ...
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bioRxiv - Cancer Biology 2020Quote: ... Human DLK1 ectodomain expression vector was obtained from Addgene (DLK1-bio-His, RRID:Addgene_51876) (Sun et al. ...
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bioRxiv - Cancer Biology 2024Quote: ... mRFP with a C-terminal KDEL sequence (ER-mRFP) was PCR amplified out of ER-mRFP (Addgene, 62236) using the forward primer 5’-GGTCGGATCCGCCGCCACCATGGACAGCAAAGG-3’ and the reverse primer 5’-GAGGCACCGGTGTTTAGAGCTCATCTTT-3’ ...
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bioRxiv - Biochemistry 2022Quote: ... The pDisplay-BirA-ER construct (Addgene #20856) was a gift from Alice Ting (Stanford University) ...
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bioRxiv - Molecular Biology 2022Quote: ... and optoPLD-ER ‘dead’ (Addgene plasmid # 140059) (Tei and Baskin ...
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bioRxiv - Neuroscience 2020Quote: ... bacterial biotin ligase BirA-ER from Addgene #20856 was subcloned into a CFP-P2A expressing shuttle vector to form f(syn)CFP-P2A-BirA-ER ...
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bioRxiv - Molecular Biology 2020Quote: ER-GCaMP6-1556 was provided by Addgene. Adenoviruses carrying mouse TUNA BNLN were generated using the ViraPowerTM adenoviral expression system (Invitrogen) ...
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bioRxiv - Cell Biology 2020Quote: ER-RFP was purchased from Addgene (62236). Yeast Ykt6 (Dharmacon ...
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bioRxiv - Cell Biology 2021Quote: ... a codon-optimized signal sequence from human ER-resident p23 was inserted into the AgeI site of p-sfGFP-N1 (Addgene #54737). p23ss-sfGFP lacking the stop codon was PCR amplified and flanked with a 3’ NheI site and TA cloned into pCRII-TOPO vector ...
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bioRxiv - Molecular Biology 2021Quote: ... DsRed-ER plasmids were purchased from Addgene (#55836). The preparation of SCAP ...
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bioRxiv - Microbiology 2019Quote: ... endoplasmic reticulum (pLV-ER GFP; Addgene plasmid #80069), Golgi apparatus (pLV-Golgi GFP ...
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bioRxiv - Cell Biology 2022Quote: ... The ER marker GFP-SEC61B (Addgene plasmid 121159) and DsRed2 ER5 (Addgene plasmid 55836 ...
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bioRxiv - Molecular Biology 2023Quote: Cepia ER plasmid was purchased from Addgene (#58215). The CEPIA1er protein was excited at 543 nm ...
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bioRxiv - Cell Biology 2024Quote: ... BFP ER-reporter was purchased from Addgene (49150).
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bioRxiv - Cancer Biology 2023Quote: The human Insulin Receptor cDNA was a gift from Frederick Stanley (Addgene plasmid # 24049; http://n2t.net/addgene:24049; RRID:Addgene_24049). This construct (hIR ...
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bioRxiv - Biochemistry 2021Quote: ... and ER lumen ([Ca2+]ER) were monitored using G-GECO1.1 (a gift from Robert Campbell from University of Alberta; Addgene plasmid #32445) (Zhao et al. ...
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bioRxiv - Biophysics 2020Quote: ... overnight dialysis and subsequent Sumo-tag cleavage by human sumo protease (His-tagged SenP1; Addgene #16356) 44 were performed in 50 mM Tris/HCl ...
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bioRxiv - Neuroscience 2020Quote: ... Constructs were co-transfected with pDisplay-BirA-ER (Addgene plasmid 20856 ...
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bioRxiv - Cancer Biology 2021Quote: ... mCherry-ER-3was a gift from Michael Davidson (Addgene plasmid # 55041 ...
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bioRxiv - Cell Biology 2022Quote: ... pLenti-PGK-ER-KRASG12V was purchased from Addgene (35635).
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bioRxiv - Cell Biology 2023Quote: ... pDisplay-BirA-ER (a gift from Alice Ting (Addgene plasmid # 20856 ...
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bioRxiv - Cell Biology 2023Quote: ... A construct encoding mCherry-ER was obtained from Addgene, and a construct encoding TMCO4-EGFP was synthesised and cloned by GeneWiz (Leipzig ...
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bioRxiv - Genetics 2024Quote: ... or the Alpha (Addgene # 170451), Beta (Addgene # 170449) ...
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bioRxiv - Cell Biology 2023Quote: ... Plasmids expressing murine PKA (mPKA) RIIα and ER membrane targeting signal fused miniTurbo promiscuous biotin ligase (ER-mTb) were obtained from Addgene (#45527 and #107174, respectively). The mPKA RIIα sequence was PCR amplified and cloned into the upstream of the mTb sequence ...
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bioRxiv - Molecular Biology 2020Quote: ... mEmerald-ER-3 was a gift from Michael Davidson (Addgene plasmid # 54082 ...