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Citations for Addgene :
1 - 50 of 157 citations for EnzyFluo ERK Phosphorylation Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2021Quote: ... as the ERK KTR iRFP (Addgene #59150) lentiviral expression vector does not confer antibiotic resistance ...
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bioRxiv - Synthetic Biology 2024Quote: ERK-KTR-mNeonGreen was subcloned from Addgene plasmid #129631 (Chavez-Abiega ...
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bioRxiv - Cell Biology 2022Quote: ... ERK-nKTR-GFP was purchased from Addgene (Addgene plasmid # 59150). C-terminally mGFP-tagged human NRAS in pLenti-C-mGFP was purchased from OriGene (OriGene cat# RC202681L2) ...
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bioRxiv - Cell Biology 2019Quote: ... We used gateway cloning40 to introduce ERK-KTR-mCer3 and ERK1-mRuby2 into PGK pLenti DEST vectors (Addgene), infected and selected the H2B-iRFP MCF10As (Blasticidin 3µg/ml and Hygromycin 10 µg/ml Corning) ...
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bioRxiv - Cell Biology 2021Quote: ... a vector for overexpression of constitutively active FOXO1 with mutated phosphorylation site (pcDNA3 Flag FKHR AAA mutant was a gift from Kunliang Guan (Addgene plasmid #13508 ...
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bioRxiv - Genetics 2019Quote: ... were ordered from Integrated DNA Technologies and after phosphorylation and annealing were cloned into pLKO.1 - TRC shRNA cloning vector (Addgene #10878) into AgeI/EcoRI site ...
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bioRxiv - Developmental Biology 2019Quote: Four gRNAs targeting loci on both sides of the C-terminal region of Rok containing the putative phosphorylation sites to be mutated were cloned into pCFD3 vector (Addgene 49410) following the protocol from (Port et al. ...
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bioRxiv - Cell Biology 2021Quote: ... EF1a-ERK-KTR-mScarlet lentiviral expression vector was generated by Gibson Assembly cloning based on an ERK-KTR-Clover plasmid from Markus Covert lab (Addgene #59150).
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bioRxiv - Biophysics 2022Quote: The plasmid encoding GPCR kinase subtype 2 used for the receptor phosphorylation in insect cells (GRK2-CAAX) was a gift from Robert Lefkowitz (Addgene plasmid #166224 49). Full-length arrestin21-418 (C150L ...
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bioRxiv - Cancer Biology 2022Quote: ... or EJ5GFP for NHEJ assay (Addgene, Plasmid 44026) were generated as previously described (20) ...
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bioRxiv - Cancer Biology 2022Quote: We employed the Traffic Light Reporter assay (Addgene #31482) to look at HR/altNEHJ efficiency ...
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bioRxiv - Genetics 2021Quote: The Downstream assay vector was based on a pSMART backbone (Addgene plasmid # 49157 ...
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bioRxiv - Microbiology 2021Quote: Plasmids for luciferase assays were purchased from Addgene (ID: 105494 – 105497) and plasmids for testing interaction in vegetative cells were constructed following previously established protocols (39 ...
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bioRxiv - Biochemistry 2022Quote: ... PRESTO Tango assay constructs were acquired from Addgene (Cat. no. 1000000068) while the Tango assay constructs were generated based on previously described framework (Barnea et al. ...
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bioRxiv - Molecular Biology 2023Quote: The following plasmids were purchased from Addgene and used for the RaPID-MS assay: BoxB-plasmid (Addgene #107253); BoxB-EDEN15 plasmid (#107252) ...
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bioRxiv - Synthetic Biology 2023Quote: ... The YTK kit (Addgene; Kit #1000000061) was used as a source for all relevant non-coding DNA sequences — including promoters and terminators ...
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bioRxiv - Biochemistry 2022Quote: Plasmid cleavage assays were performed using the eGFP-hAgo2 plasmid (Addgene #21981) as target DNA ...
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bioRxiv - Synthetic Biology 2022Quote: ... MoClo Plant Parts Kit (Addgene Kit #1000000047), GreenGate Cloning System (Addgene Kit #1000000036 ...
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bioRxiv - Cancer Biology 2022Quote: HN30 and HN31 cell lines with pDRGFP for HR assay (Addgene, Plasmid #26475) or EJ5GFP for NHEJ assay (Addgene ...
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bioRxiv - Molecular Biology 2023Quote: ... The R6K plasmid for mate-out transposition assays was obtained from Addgene (#64968) [66] ...
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bioRxiv - Neuroscience 2023Quote: The generation of plasmids for this assay was previously described and obtained from Addgene [7] ...
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bioRxiv - Neuroscience 2023Quote: The plasmid RHCglo used for minigene assay was a gift from Thomas Cooper (Addgene plasmid #80169 ...
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bioRxiv - Microbiology 2020Quote: ... Cas9 activity was checked using the XPR_047 assay (a gift from David Root, Addgene 107145) and was 79.5% and >83.4% ...
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bioRxiv - Neuroscience 2023Quote: ... Chimeric G proteins for the Gsx assay 44 were obtained from Addgene (Watertown, MA, USA).
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bioRxiv - Systems Biology 2022Quote: ... and lambda phosphatase were purchased from Addgene (Addgene Kit #1000000094)7.
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bioRxiv - Microbiology 2021Quote: ... WT Cas9 activity was checked using the XPR_047 assay (a gift from David Root, Addgene 107145) and was always >80-90% ...
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bioRxiv - Cancer Biology 2021Quote: ... Reporter assays to assess RUNX1 transcriptional activity were done using the pMCSF-R-luc plasmid (Addgene plasmid #12420 ...
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bioRxiv - Cancer Biology 2022Quote: The mismatch repair assay was performed as described previously57 using plasmids pGEM5Z (+)-EGFP (Addgene, cat #65206) and p189 (Addgene ...
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bioRxiv - Cell Biology 2022Quote: Luciferase assays were performed in HEK293T cells using the YAP/TAZ reporter 8xGTIIC-lux (Addgene, 34615). Renilla-Luc (0.05 µg/well ...
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bioRxiv - Biochemistry 2023Quote: A Halo-based assay to measure mitophagy was recently developed using pSu9-Halo-mGFPplasmid (Addgene, 184905) 53 ...
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bioRxiv - Genomics 2022Quote: ... elegans Vector Kit was a gift from Andrew Fire (Addgene kit # 1000000001). A translational reporter was later generated by synthesizing the remainder of the coding sequence (IDT ...
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bioRxiv - Biochemistry 2020Quote: ... and for luciferase assays a ratio of 1:9:10 HA-Clover plasmid:pcDNA(-):HRE-Luciferase (Addgene #26731) was used (physiological expression levels) ...
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bioRxiv - Cell Biology 2023Quote: The photoactivation-based fusion assay (Karbowski et al., 2014) was conducted by expressing Mito-PAGFP (Addgene #23348) in primary human fibroblasts ...
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bioRxiv - Synthetic Biology 2023Quote: ... We used DNA parts provided with the EMMA cloning kit (Addgene kit # 1000000119) and generated DNA fragments by PCR using Platinum™ SuperFi II Green PCR Master Mix (ThermoFisher ...
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bioRxiv - Neuroscience 2023Quote: The plasmid RHCglo used for minigene assay was a gift from Thomas Cooper (Addgene plasmid #80169 ; http://n2t.net/addgene:80169 ; RRID:Addgene_80169) (49) ...
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bioRxiv - Neuroscience 2023Quote: ... Astrocytes were labeled for live assays prior to incorporation into miBrains using an AAV (pAAV-CAG-tdTomato, Addgene or AAV9-CAG-GFP ...
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bioRxiv - Developmental Biology 2024Quote: ... The AAV-CMV-GFP plasmid used for neurite outgrowth assay was a gift from Connie Cepko (Addgene, #67634).
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bioRxiv - Synthetic Biology 2023Quote: The CIDAR MoClo Parts Kit was a gift from Douglas Densmore (Addgene kit # 1000000059). CIDAR MoClo Extension ...
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bioRxiv - Biochemistry 2022Quote: ... The PRESTO-Tango plasmid kit was a gift from Bryan Roth (Addgene kit # 1000000068).
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bioRxiv - Synthetic Biology 2023Quote: ... The MoClo-YTK plasmid kit was a gift from John Dueber (Addgene kit # 1000000061). Enzymes used were from New England Biolabs unless stated otherwise ...
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bioRxiv - Molecular Biology 2023Quote: The CRISPaint gene tagging kit was a gift from Veit Hornung (Addgene kit # 1000000086). To generate a targeting construct for AGO2 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... All GPCR plasmids originated from the Roth lab PRESTO-Tango kit (Addgene, Kit #1000000068) and were amplified in E ...
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bioRxiv - Neuroscience 2020Quote: ... Feng Zhang (Addgene kit #1000000019). Once assembled into a destination vector ...
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bioRxiv - Bioengineering 2021Quote: ... The GoldenPiCS Kit was a gift from the Gasser/Mattanovich/Sauer group (Addgene kit #1000000133). All coding sequences were amplified with high-fidelity Phusion DNA Polymerase (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2020Quote: ... Basic DNA parts were selected from GoldenBraid 2.0 kit from Diego Orzaez (Addgene kit # 1000000076) or MoClo Toolkit ...
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bioRxiv - Developmental Biology 2023Quote: TALEN plasmids were constructed using the Platinum Gate TALEN Kit (Kit #1000000043, Addgene, Cambridge, MA) as previously described (Sakuma et al. ...
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bioRxiv - Cancer Biology 2019Quote: Reporter assays were performed as described previously (Ferreira et al., 2017) using the DR5 reporter construct obtained from Addgene (Plasmid 16012 (Takimoto & El-Deiry ...
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bioRxiv - Biochemistry 2023Quote: Luciferase reporter assays were performed in MEF cells transfected with a UCP3 reporter plasmid[18] (UCP3 EP1, Addgene #71743) or PGC-1α 2kb promoter (Handschin et al ...
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bioRxiv - Neuroscience 2020Quote: The TALEN kit used for TALE assembly was a gift from Keith Joung (Addgene kit # 1000000017). DNA fragments encoding ROSA TALEN repeat arrays were cloned into plasmid pJDS71 ...
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bioRxiv - Plant Biology 2024Quote: We used zCas9i cloning kit: MoClo-compatible CRISPR/Cas9 cloning kit with intronized Cas9 (AddGene #1000000171). Sequence of all oligonucleotides and PCR primers used in this study are provided in Table S1 ...