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Citations for Addgene :
1 - 50 of 446 citations for DNA Standards since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2021Quote: sgRNAs were purchased as single-stranded DNA oligos (25nmoles standard desalted, IDT) with BbsI sticky ends and cloned in pairs into the pDG459 plasmid (Addgene #10090) for the deletion step using a one-step cloning reaction as described by the Thomas lab 25 The same protocol with the minor modification of replacing the second oligo with water during ligation was used to clone the syn-sgRNAs into pX459 (Addgene #62988) ...
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bioRxiv - Cell Biology 2023Quote: ... using a standard protocol from Addgene.
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bioRxiv - Cell Biology 2020Quote: ... or with pEGFP-CenpA-IRES-neo (Addgene, LGC Standards, Teddington, UK) using JetPEI as transfection reagent (Polyplus transfection ...
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bioRxiv - Biophysics 2021Quote: ... and a standard transgene cargo plasmid pAAV-CAG-GFP (Addgene #37825) were co-transfected to adherent HEK293T/17 cells at a mass ratio of 4:2:1 ...
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bioRxiv - Immunology 2021Quote: B16F10 cells were transduced using standard lentivirus transduction protocols (see Addgene protocol found at ...
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bioRxiv - Microbiology 2019Quote: ... was generated using standard cloning techniques and is available from Addgene.
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bioRxiv - Cell Biology 2020Quote: ... Stable cell lines were created by using a standard lentiviral production protocol (Addgene). Briefly ...
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bioRxiv - Biophysics 2020Quote: ... we stably integrated tdMCP-tdGFP and OsTIR1 into cells using a standard lentiviral production protocol (Addgene). For lentivirus production ...
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bioRxiv - Cell Biology 2019Quote: ... Annealed oligonucleotide with gRNA targeting sequence was cloned into px330 plasmid by a standard subcloning method (Addgene plasmid ...
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bioRxiv - Cancer Biology 2019Quote: ... Knockdown experiments with shRNA lentiviruses were conducted according to the standard lentivirus package and transduction protocols from Addgene. These pLKO-based lentiviral shRNA plasmids were co-transfected with packaging plasmids (psPAX2 and pMD2.G ...
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bioRxiv - Cell Biology 2020Quote: Transfection and selection: SKOV3 cells were transfected with pH2B-mCherry-IRES-puro2 plasmid (Addgene, LGC Standards, Teddington, UK) or with pEGFP-CenpA-IRES-neo (Addgene ...
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bioRxiv - Cancer Biology 2023Quote: ... or standard cloning in the pTRIPZ plasmid from pTK-Slug (a gift from Robert Weinberg, Addgene plasmid # 36986) (Guo et al. ...
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bioRxiv - Genetics 2023Quote: ... Viruses were produced according to standard procedures using commercially available plasmids (pMD2.G Addgene #12259, psPAX Addgene #12260). Briefly ...
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bioRxiv - Genetics 2023Quote: ... Viruses were produced according to standard procedures using commercially available plasmids (pMD2.G Addgene #12259, psPAX Addgene #12260). Briefly ...
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bioRxiv - Cell Biology 2021Quote: ... lentivector DNA plasmids (Addgene plasmids # 27150 ...
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bioRxiv - Neuroscience 2019Quote: ... and used to package lentiviral particles according to standard protocols with third-generation packaging plasmids (pMDlg-pRRE, pRSV-ReV, pMD2.0; Addgene). Lentiviral backbone and packaging plasmids were transfected into HEK293T cells using Lipofectamine 2000 and Lentivirus-containing medium was harvested after 48h ...
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bioRxiv - Cancer Biology 2022Quote: ... 2μg of DNA (pX330 [Addgene # 42230] - sgFBXW7 or sgAPC ...
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bioRxiv - Cell Biology 2023Quote: The following DNA constructs (Addgene) were used in this study ...
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bioRxiv - Neuroscience 2020Quote: ... and pAAV-CaMKIIa-mCherry plasmids were generated by the University of Minnesota Viral Vector and Cloning Core (VVCC; Minneapolis, MN) using standard cloning techniques and pAAV-CaMKIIα-hChR2(C128S/D156A)-mCherry (Addgene plasmid #35502 ...
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bioRxiv - Biophysics 2020Quote: ... tdMCP-tdGFP and one of the TALE-TF with additional copies of the gene construct via lentiviral gene transfer using a standard lentiviral production protocol (Addgene). We quantified the number of integrations by comparing the levels of transcription activation before and after lentiviral transfection of additional copies of the gene construct.
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bioRxiv - Cell Biology 2022Quote: ... MLKL gene and its truncated version MLKL(1-182) were amplified by standard PCR from pRetrX-TRE3G-hMLKL-Venus (Addgene_106078) using primers MLKL_Fw and MLKL_Rv for the first ...
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bioRxiv - Neuroscience 2023Quote: Whole-cell patch-clamp recordings were made from SPNs as standard in striatal slices of animals previously injected with AAV1-CaMKIIa-hChR2(H134R)-eYFP (Lee et al., 2010) (Addgene) in either mPFC or the visual cortex ...
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bioRxiv - Cell Biology 2023Quote: The pCS2 Notch1 full length-6MT and pCS2 Notch1 ICv-6MT plasmids were purchased from Addgene (LGC Standards, Teddington, UK) as bacterial stabs ...
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bioRxiv - Bioengineering 2020Quote: ... the secondary nicking sgRNA diluted duplex was ligated into a standard U6 sgRNA vector (such as the lentiGuide-Puro vector; Addgene #52963). Note that when using alternative plasmids for performing pegRNA/sgRNA cloning ...
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bioRxiv - Biophysics 2021Quote: ... HeLa cells were stably engineered to display a doxycycline-inducible GFP fused to a mouse CD80 transmembrane domain using standard second-generation lentivector production protocols and the plasmids pMD2G (Addgene 12259), pCMVR8.74 (Addgene 22036) ...
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bioRxiv - Genetics 2022Quote: ... sgRNA oligos with BbsI sticky ends (IDT, 25nmole standard desalted, Supplementary Table 1) were ligated into the pDG459 plasmid (Addgene 100901) as previously described 45 ...
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bioRxiv - Bioengineering 2023Quote: CHO cells were electroporated with 2 μg of plasmids containing the primary or a fragment of the primary microRNA sequences of three miRs that were in high abundance in standard or stressed cultures (miR-21 (Addgene #21114), miR-92a (Addgene #46672) ...
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bioRxiv - Plant Biology 2023Quote: ... L0 parts were synthesised either by GENEWIZ or Twist Bioscience following the standard syntax for plant synthetic biology with PROM5 or PROM and 5UTR overhangs and cloned into the plasmid pUAP1 (Addgene #63674) (Patron et al. ...
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bioRxiv - Cell Biology 2023Quote: Lentiviral particles were prepared in HEK293T cells using standard calcium-phosphate transfection of the lentivector with packaging plasmids pMD2.G (Trono lab, Addgene #12259) and pCMV-dR8.74 (Trono lab ...
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bioRxiv - Neuroscience 2020Quote: ... mRuby2 DNA was purchased from Addgene (mRuby2-C1 ...
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bioRxiv - Bioengineering 2022Quote: ... Plasmid DNA (pm-mCherry-N1, Addgene) was labelled with Label It DNA kit (Mirus) ...
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bioRxiv - Biophysics 2022Quote: ... media was refreshed with standard growth media and cells were co-transfected with SNAP-mGluR2 (no FLAG-tag) and chimeric G protein (Gqo5, Addgene plasmid #24500) (1:2 w/w ...
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bioRxiv - Systems Biology 2020Quote: ... Cells were transformed by standard lithium acetate methods to fluorescently labelled indicated organelles with monomeric Kusibara Orange 2 (mKO2) obtained from Addgene (Cambridge, MA). Peroxisomes in strains engineered with deletions of DNM1 ...
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bioRxiv - Synthetic Biology 2020Quote: ... Component vectors containing markers and connectors used to construct cloning vectors using this standard were a gift from John Dueber distributed through (Kit # 1000000061, Addgene, Watertown, U.S.A.). The genes or gene fragments were cloned into the storage vector pYTK001 using a Golden Gate assembly with BsmBI ...
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bioRxiv - Molecular Biology 2023Quote: ... values were converted into copy numbers by measuring against a standard curve of the AAV transfer plasmid or the Ai9 plasmid (a gift from H. Zeng Addgene plasmid # 22799). The AAV genome copy number was divided by the number of nuclei to normalize for tissue input.
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bioRxiv - Molecular Biology 2023Quote: Firefly and Renilla luciferase expression plasmids were cloned by standard methods starting with the pGL3 PUb-luc plasmid described previously (7) and pSLfa-PUb-MCS (Addgene plasmid # 52908). Transgenesis plasmids were generated using NEBuilder HiFi Assembly Master Mix (NEB ...
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bioRxiv - Cell Biology 2023Quote: A PIP-FUCCI coded DNA fragment was PCR amplified with template DNA (pENTR-PIP-FUCCI; Addgene; plasmid #118621), which was a gift from Jean Cook ...
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bioRxiv - Biochemistry 2020Quote: ... BacMam viral plasmids & production was performed by Oxford Expression Technologies (OET) using the template DNA from Addgene plasmids #89470 & #89471 ...
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bioRxiv - Microbiology 2023Quote: ... and VSV-G DNA (Addgene, cat. 8454) (24 ...
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bioRxiv - Cell Biology 2023Quote: The following DNA constructs purchased from Addgene were used ...
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bioRxiv - Molecular Biology 2022Quote: ... baculovirus gp64 signal sequence under control of the very late polyhedrin promoter was inserted into the insect cell vector plasmid pACEBac1 (Addgene, LGC Standards Teddington, UK) by using another synthetic gene (VK18 ...
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bioRxiv - Cancer Biology 2020Quote: ... and/or Sleeping Beauty (SB)-transposon plasmid DNA together with 10μg of hyperactive PB Transposase plasmid DNA (hyPBase) or hyperactive Sleeping Beauty transposase (pCMVT7-SB100, Addgene 34879) were diluted in sterile Ringer’s solution in a volume corresponding to 10% of the body weight (on average ...
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bioRxiv - Genetics 2020Quote: ... These plasmids were constructed through multiple rounds of cloning using DNA fragments from yeast DNA or plasmids acquired from Addgene (kind gifts from John McCusker ...
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bioRxiv - Cancer Biology 2021Quote: ... Frequency and type of mutations were determined by cloning the OP9 genomic DNA fragment spanning 600 bp around the gRNA DNA break site into pUC19 plasmid (#50005, AddGene) and sequencing 50 single colonies (primers ...
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bioRxiv - Biochemistry 2023Quote: ... 8 µg psPAX2 packaging plasmid DNA and 1 µg pMD2.G envelope plasmid DNA (both gifts from Didier Trono, Addgene plasmid no ...
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bioRxiv - Immunology 2021Quote: ... 3 μg of pIRES-eGFP plasmid DNA (Addgene) were digested with 250 U of Benzonase ...
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bioRxiv - Neuroscience 2020Quote: ... The following DNA plasmids were obtained from Addgene: pGEMTEZ-TeTxLC (Addgene plasmid #32640 ...
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bioRxiv - Developmental Biology 2022Quote: ... the DNA vector pT3TS-nCas9n (Addgene plasmid #46757) was linearized by XbaI digestion and purified ...
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bioRxiv - Cell Biology 2020Quote: ... per μg plasmid DNA with lentiCRISPR_V2 (Addgene 52961): gag/pol (Addgene 14887) ...
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bioRxiv - Immunology 2020Quote: ... pyogenes chimeric gRNA DNA template (plasmid #51132; Addgene) while the reverse primer was complementary to the gRNA DNA template (see Table I for primer sequences) ...