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Citations for Addgene :
1 - 50 of 85 citations for Cytomegalovirus Purified Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... rat Nfasc gene expressed from the cytomegalovirus (CMV) promoter (a gift from Vann Bennett, Addgene plasmid # 31061; http://n2t.net/addgene:31061; RRID: Addgene_31061) (Zhang et al. ...
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bioRxiv - Neuroscience 2022Quote: ... This construct was generated from a plasmid that contained a wild-type (WT) rat Nfasc gene expressed from the cytomegalovirus (CMV) promoter (a gift from Vann Bennett, Addgene plasmid # 31061 ...
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bioRxiv - Neuroscience 2021Quote: ... and SV40T antigen (Addgene Plasmid #21826) were obtained from Addgene.
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bioRxiv - Biochemistry 2021Quote: ... SV40 T antigen lentiviral plasmid (Addgene #22298) was packaged in HEK 293T cells using helper plasmids pMD2.G (Addgene #12259 ...
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bioRxiv - Microbiology 2020Quote: ... Her2 antigen was acquired from Addgene (pCDNA3). CD99 (GenBank ...
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bioRxiv - Molecular Biology 2021Quote: ... and Large T SV40 antigen (Addgene plasmid # 21826) as described ...
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bioRxiv - Molecular Biology 2022Quote: ... and 3 µg pBABE-neo largeT antigen cDNA (Addgene, 1780 ref (16))using lipofectamine 2000 (Invitrogen ...
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bioRxiv - Bioengineering 2020Quote: ... The antigen-binding fragments (Fab) were cloned into pHLSec vector (Addgene, #99845) using AgeI and XhoI restriction sites ...
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bioRxiv - Biochemistry 2024Quote: ... The SV40 Large T antigen and HA-TRIM71 were purchased from Addgene (plasmid # 136616 and #52717 ...
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bioRxiv - Immunology 2024Quote: ... MEFs were transfected with SV40 small+large T antigen-expressing plasmid (22298, Addgene) using Lipofectamine 2000 (11668019 ...
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bioRxiv - Cell Biology 2020Quote: ... SV40 Large T antigen CDS was subcloned from pBABE-puro-SV40 LgT plasmid (Addgene 13970) into LeGO iG vector between BamHI and EcoRI sites ...
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bioRxiv - Neuroscience 2021Quote: ... MEFs were then expanded to 6-well plates and transiently transfected with SV40T antigen (Addgene) and maintained until stably proliferative ...
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bioRxiv - Cell Biology 2022Quote: ... cells were transduced by thermosensitive retroviral gene transfer of SV40 T antigen (LOX-CW-CRE, Addgene) at a MOI of 5 in Proliferation UREC medium with 8μg/mL polybrene (64) ...
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bioRxiv - Genomics 2020Quote: Purified PX330 (Addgene) vector DNA was fully digested with EcoRV (New England Biolabs ...
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bioRxiv - Genomics 2021Quote: ... Early passage primary MEFs were transformed with SV-40 T antigen containing plasmid pBSSVD2005 (ADDGENE, Cambridge, MA) to generate immortalized MEFs ...
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bioRxiv - Microbiology 2019Quote: ... They were immortalized by transduction with a retroviral vector encoding SV40 large T antigen (pBABE-zeo largeTcDNA, Addgene). The Atf6+/+ vs ...
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bioRxiv - Cell Biology 2019Quote: ... Primary MEFs were then immortalized by transient transfection with a plasmid expressing SV40 large T antigen (Addgene #21826). Single cell clones were then isolated by limiting dilution ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were immortalized by transduction with a retrovirus expressing SV40 large T antigen from pBABE-puro largeTcDNA (Addgene plasmid # 14088 ...
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bioRxiv - Cell Biology 2021Quote: Embryonic fibroblasts were generated from RHBDL4 WT and RHBDL4 KO E14.5 embryos and immortalized using lentiviral transduction of SV40 virus large T antigen (Ef1a_Large T-antigen_Ires_Puro, Addgene plasmid 18922), as described by Christova et al ...
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bioRxiv - Cancer Biology 2022Quote: ... and SV40-T/t antigens expressing lentiviruses using vectors pLenti CMV-RasV12-Neo (w108-1) (HRAS G12V, #22259, Addgene), and pLenti-CMV/TO-SV40 small + Large T (w612-1 ...
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bioRxiv - Cancer Biology 2019Quote: Arachnoid cells were immortalized using a lentivirus harboring the SV40 large T antigen [pBABE-puro SV40 LT was a gift from Thomas Roberts (Addgene, RRID:Addgene_13970) as previously described (48) ...
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bioRxiv - Molecular Biology 2022Quote: ... early passage primary MEFs from all genotypes were infected in parallel with the SV40 large T antigen (Addgene:13970) (Zhao et al. ...
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bioRxiv - Biophysics 2021Quote: ... and immortalized by transduction with a retrovirus expressing SV40 T antigen from pBABE-puro SV40LT (pBABE-puro SV40LT was a gift from Thomas Roberts (Addgene plasmid # 13970 ...
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bioRxiv - Biophysics 2023Quote: ... The DNA fragment encoding the T-cell-restricted intracellular antigen-1 (TIA-1) was digested from pFRT-TO-eGFP-TIA1 (#106094, Addgene) using Bsp1407I (TaKaRa ...
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bioRxiv - Cancer Biology 2024Quote: The chimeric antigen receptor against human CD276 (Du et al., 2019) and CD19 (Maude et al., 2018)together with the Thy1.1 surface antigen (Addgene 154194)(Umkehrer et al. ...
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bioRxiv - Cell Biology 2023Quote: ... Non-GFP expressing ZT cells were obtained by sorting out non-fluorescence ZT cells and they were subsequently immortalized by transfecting them with SV40 T-antigen expressing plasmid (Addgene).
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bioRxiv - Cancer Biology 2019Quote: Arachnoid cells were immortalized using a lentivirus harboring the SV40 large T antigen [pBABE-puro SV40 LT was a gift from Thomas Roberts (Addgene, RRID:Addgene_13970) as previously described (48) ...
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bioRxiv - Molecular Biology 2023Quote: ... WT and T1150A catalytic domains tagged with nuclear localization sequence (NLS) of the SV40 Large T-antigen were cloned into the mVenus-C1 plasmid backbone (provided by Steven Vogel, Addgene plasmids no. 27794) (Koushik et al. ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were immortalized by transduction with a retrovirus expressing SV40 large T antigen from pBABE-puro largeTcDNA (Addgene plasmid # 14088; http://n2t.net/addgene:14088; RRID:Addgene_14088; a gift from William Hahn) as previously described (13) ...
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bioRxiv - Cancer Biology 2024Quote: ... was amplified and purified as directed by Addgene. Lentiviral particles were generated by transfecting HEK293T cells with the GeCKOv2 CRISPR Knockout Pooled Plasmid Library ...
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bioRxiv - Molecular Biology 2022Quote: WT SpCas9 was purified using pET-Cas9-NLS-6xHis (Addgene #62933)7 plasmid ...
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bioRxiv - Neuroscience 2022Quote: ... Purified and concentrated adeno-associated viruses (AAV) were purchased from Addgene. Cre-inducible ChR2-mCherry was used (AAV-EF1α-DIO-hChR2(H134R)-mCherry ...
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bioRxiv - Cell Biology 2023Quote: ... and DNA was purified before ligation into pJR103 (Addgene plasmid #187242). The ligated DNA was precipitated using isopropanol (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2021Quote: ... the 24xMS2 stem loop sequence was purified from pCR-24xMS2L-stable (Addgene_31865) by restriction digest and the 128xMS2 from pMK123-128xMS2 (Tantale et al. ...
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bioRxiv - Neuroscience 2019Quote: ... Then AAV were purified using Iodixanol Gradient Ultracentrifugation as described by Addgene (https://www.addgene.org/protocols/aav-purification-iodixanol-gradient-ultracentrifugation/) ...
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bioRxiv - Microbiology 2022Quote: ... Five hundred nanograms of purified spectinomycin-resistance conferring plasmid pDL278 (Addgene, #46882) were also added to each culture ...
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bioRxiv - Neuroscience 2022Quote: ... Then AAVs were purified using Iodixanol Gradient Ultracentrifugation as described by Addgene (https://www.addgene.org/protocols/aav-purification-iodixanol-gradient-ultracentrifugation/) ...
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bioRxiv - Biochemistry 2022Quote: ... purified His14-Avi-SUMOEu1-anti GFP nanobody (expressed from pTP396, Addgene #149336)74 was biotinylated using BirA (expressed from pTP264 ...
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bioRxiv - Genomics 2023Quote: ... The transposase was purified after expressed from the pTXB1-Tn5 plasmid (Addgene). Comparable results can be generated with purchasable Tn5 transposase (Vazyme Biotech co.,ltd) ...
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bioRxiv - Molecular Biology 2019Quote: ... The nanodisc scaffold protein MSP1D1 was expressed and purified using pMSP1D1 (Addgene #20061) as described previously (Denisov et al. ...
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bioRxiv - Molecular Biology 2019Quote: ... Purified P16-TET pTRIPZ plasmid was mixed with VSVG and Δ8.9 (Addgene, USA) to prepare lentivirus transfection particles ...
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bioRxiv - Microbiology 2020Quote: ... and the purified product was introduced by In-Fusion into pMSP3545 (Addgene #46888) digested with SpeI and NcoI ...
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bioRxiv - Molecular Biology 2021Quote: ... previously purified in-house 80 using a commercially purchased plasmid (Addgene plasmid pRK739), and dialyzed in 20K molecular weight cut-off (MWCO ...
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bioRxiv - Developmental Biology 2020Quote: ... HEK 293 cells were transfected with purified pCI-HA-YAP plasmid (Cat #27007, Addgene). DharmaFECT (Cat #T2002-02 ...
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bioRxiv - Plant Biology 2021Quote: ... Gel-purified PCR product and SspI-digested pET-His6-MBP-TEV-LIC vector (Addgene) were treated with T4 DNA polymerase with 25 mM dCTP and dGTP for 30 min at 22°C followed by heat inactivation ...
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bioRxiv - Cell Biology 2023Quote: ... purified from bacteria cells transformed with the plasmid pET-28b-RfxCas13d-His (Addgene 141322), was kindly provided by JP Concordet ...
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bioRxiv - Molecular Biology 2020Quote: ... Purified amplicons were mixed (30 ng each) with 50 ng pJET1.2-STOP-dsRed (51) (Addgene plasmid # 60944 ...
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bioRxiv - Neuroscience 2022Quote: ... One microliter of endo-free purified (2 ug/ul) pCAG-EGFP plasmid (Addgene, cat# 89684) mixed with 0.05% Fast Green (Sigma ...
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bioRxiv - Neuroscience 2022Quote: The Kdm6a Enzyme-Dead (Kdm6a DeM-dead) plasmid was purified and validated by Addgene (#40619) in which alanine (A ...
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bioRxiv - Cell Biology 2022Quote: Full-length ERK3 wild type (WT) pDONR-223 construct purified from Human Kinase Library (Addgene) was used as a template to generate ERK3 K49A K50A kinase dead (KD ...