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Citations for Addgene :
1 - 50 of 1741 citations for Cytomegalovirus Cell Lysate Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... rat Nfasc gene expressed from the cytomegalovirus (CMV) promoter (a gift from Vann Bennett, Addgene plasmid # 31061; http://n2t.net/addgene:31061; RRID: Addgene_31061) (Zhang et al. ...
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bioRxiv - Neuroscience 2022Quote: ... This construct was generated from a plasmid that contained a wild-type (WT) rat Nfasc gene expressed from the cytomegalovirus (CMV) promoter (a gift from Vann Bennett, Addgene plasmid # 31061 ...
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bioRxiv - Neuroscience 2021Quote: ... and SV40T antigen (Addgene Plasmid #21826) were obtained from Addgene.
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bioRxiv - Biochemistry 2021Quote: ... SV40 T antigen lentiviral plasmid (Addgene #22298) was packaged in HEK 293T cells using helper plasmids pMD2.G (Addgene #12259 ...
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bioRxiv - Microbiology 2020Quote: ... Her2 antigen was acquired from Addgene (pCDNA3). CD99 (GenBank ...
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bioRxiv - Cell Biology 2022Quote: ... cells were transduced by thermosensitive retroviral gene transfer of SV40 T antigen (LOX-CW-CRE, Addgene) at a MOI of 5 in Proliferation UREC medium with 8μg/mL polybrene (64) ...
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bioRxiv - Molecular Biology 2021Quote: ... and Large T SV40 antigen (Addgene plasmid # 21826) as described ...
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bioRxiv - Cancer Biology 2024Quote: ... Whole cell lysates (200 µg) were incubated with GST-RAF-RBD (Addgene) bound to glutathione agarose beads at 4°C for 1 hour ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were immortalized by transduction with a retrovirus expressing SV40 large T antigen from pBABE-puro largeTcDNA (Addgene plasmid # 14088 ...
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bioRxiv - Biophysics 2023Quote: ... The DNA fragment encoding the T-cell-restricted intracellular antigen-1 (TIA-1) was digested from pFRT-TO-eGFP-TIA1 (#106094, Addgene) using Bsp1407I (TaKaRa ...
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bioRxiv - Cell Biology 2023Quote: ... Non-GFP expressing ZT cells were obtained by sorting out non-fluorescence ZT cells and they were subsequently immortalized by transfecting them with SV40 T-antigen expressing plasmid (Addgene).
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bioRxiv - Molecular Biology 2022Quote: ... and 3 µg pBABE-neo largeT antigen cDNA (Addgene, 1780 ref (16))using lipofectamine 2000 (Invitrogen ...
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bioRxiv - Bioengineering 2020Quote: ... The antigen-binding fragments (Fab) were cloned into pHLSec vector (Addgene, #99845) using AgeI and XhoI restriction sites ...
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bioRxiv - Biochemistry 2024Quote: ... The SV40 Large T antigen and HA-TRIM71 were purchased from Addgene (plasmid # 136616 and #52717 ...
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bioRxiv - Immunology 2024Quote: ... MEFs were transfected with SV40 small+large T antigen-expressing plasmid (22298, Addgene) using Lipofectamine 2000 (11668019 ...
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bioRxiv - Cell Biology 2020Quote: ... SV40 Large T antigen CDS was subcloned from pBABE-puro-SV40 LgT plasmid (Addgene 13970) into LeGO iG vector between BamHI and EcoRI sites ...
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bioRxiv - Neuroscience 2021Quote: ... MEFs were then expanded to 6-well plates and transiently transfected with SV40T antigen (Addgene) and maintained until stably proliferative ...
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bioRxiv - Genomics 2021Quote: ... Early passage primary MEFs were transformed with SV-40 T antigen containing plasmid pBSSVD2005 (ADDGENE, Cambridge, MA) to generate immortalized MEFs ...
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bioRxiv - Microbiology 2019Quote: ... They were immortalized by transduction with a retroviral vector encoding SV40 large T antigen (pBABE-zeo largeTcDNA, Addgene). The Atf6+/+ vs ...
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bioRxiv - Cell Biology 2019Quote: ... Primary MEFs were then immortalized by transient transfection with a plasmid expressing SV40 large T antigen (Addgene #21826). Single cell clones were then isolated by limiting dilution ...
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bioRxiv - Cell Biology 2021Quote: Embryonic fibroblasts were generated from RHBDL4 WT and RHBDL4 KO E14.5 embryos and immortalized using lentiviral transduction of SV40 virus large T antigen (Ef1a_Large T-antigen_Ires_Puro, Addgene plasmid 18922), as described by Christova et al ...
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bioRxiv - Cancer Biology 2022Quote: ... and SV40-T/t antigens expressing lentiviruses using vectors pLenti CMV-RasV12-Neo (w108-1) (HRAS G12V, #22259, Addgene), and pLenti-CMV/TO-SV40 small + Large T (w612-1 ...
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bioRxiv - Cancer Biology 2019Quote: Arachnoid cells were immortalized using a lentivirus harboring the SV40 large T antigen [pBABE-puro SV40 LT was a gift from Thomas Roberts (Addgene, RRID:Addgene_13970) as previously described (48) ...
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bioRxiv - Molecular Biology 2022Quote: ... early passage primary MEFs from all genotypes were infected in parallel with the SV40 large T antigen (Addgene:13970) (Zhao et al. ...
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bioRxiv - Biophysics 2021Quote: ... and immortalized by transduction with a retrovirus expressing SV40 T antigen from pBABE-puro SV40LT (pBABE-puro SV40LT was a gift from Thomas Roberts (Addgene plasmid # 13970 ...
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bioRxiv - Cancer Biology 2024Quote: The chimeric antigen receptor against human CD276 (Du et al., 2019) and CD19 (Maude et al., 2018)together with the Thy1.1 surface antigen (Addgene 154194)(Umkehrer et al. ...
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bioRxiv - Cancer Biology 2019Quote: Arachnoid cells were immortalized using a lentivirus harboring the SV40 large T antigen [pBABE-puro SV40 LT was a gift from Thomas Roberts (Addgene, RRID:Addgene_13970) as previously described (48) ...
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bioRxiv - Molecular Biology 2023Quote: ... WT and T1150A catalytic domains tagged with nuclear localization sequence (NLS) of the SV40 Large T-antigen were cloned into the mVenus-C1 plasmid backbone (provided by Steven Vogel, Addgene plasmids no. 27794) (Koushik et al. ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were immortalized by transduction with a retrovirus expressing SV40 large T antigen from pBABE-puro largeTcDNA (Addgene plasmid # 14088; http://n2t.net/addgene:14088; RRID:Addgene_14088; a gift from William Hahn) as previously described (13) ...
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bioRxiv - Systems Biology 2022Quote: ... and added undiluted to K562 cells for a final cell concentration of 3-4 x 105 cells/mL for pJT126-based effector recruitment vectors (Addgene #161926) or 1-2 x 105 cells/mL for pCL040-based inducible protein expression vectors (to be deposited on Addgene ...
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bioRxiv - Cancer Biology 2024Quote: ... Knockout cells generated in 293A and HeLa cells were created with pLentiCRISPRv2 (Addgene, #52961) containing indicated gRNAs (Figure 7-figure supplement 1) ...
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bioRxiv - Neuroscience 2022Quote: ... HEK293T cells (#240073, Addgene, USA) were used for viral production ...
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bioRxiv - Biochemistry 2021Quote: ... Electrocompetent cells were prepared from frozen cell stocks and transformed with the pFREE vector (Addgene plasmid #92050 ...
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bioRxiv - Cell Biology 2019Quote: Stable Cas9-expressing cells were generated by transducing BeWo cells with lentiCAS9-blast (Addgene, cat. 52962). To generate TMEM16F/ANO6 knockout cells ...
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bioRxiv - Systems Biology 2020Quote: ... MDCKI + bPAC cells were generated by infecting MDCKI cells with pLenti-PGK-bPAC::NES (Addgene #130267). PKAc expressing cells were obtained by transfecting MDCKI and MDCKI + bPAC cells with pPB-CAG-PRKACA::mRuby2 (Addgene #130268) ...
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bioRxiv - Cell Biology 2019Quote: For generation of lentiviral knockout and overexpression cells HEK293T cells were transfected with psPAX2 (Addgene #12260) and pMD2.G (Addgene #12259 ...
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bioRxiv - Immunology 2022Quote: ... KMS11-Cas9+ cells and LP1-Cas9+ cells (transduced with pLX 311-Cas9 construct, Addgene plasmid # 96924) were obtained from the Broad Institute ...
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bioRxiv - Genomics 2022Quote: ... and NIH/3T3 cells (Addgene #138149), as well as doxycycline-inducible nuclease-inactive RfxdCas13d-NLS HEK293FT ...
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bioRxiv - Microbiology 2020Quote: ... and AdEasier-1 cells (Addgene #16399) were a gift from Bert Vogelstein (He et al ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells harboring plasmid pKM208 (Addgene #13077) were cultured at 30°C to an O.D ...
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bioRxiv - Microbiology 2023Quote: ... Electrocompetent cells containing pREDCas9 (Addgene #71541) were generated as previously described[68] ...
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bioRxiv - Synthetic Biology 2023Quote: ... coli MG1655 K12 cells (Addgene #37854) transformed with reporter ...
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bioRxiv - Immunology 2023Quote: ... Phoenix-Eco cells (Addgene CRL-3214) were used for retrovirus production ...
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bioRxiv - Cell Biology 2020Quote: ... endogenous SETD2 was immunoprecipitated using whole-cell extracts from HEK293T cells expressing mCherry-ACTB (Addgene plasmid #54966). Similarly ...
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bioRxiv - Microbiology 2023Quote: ... IMR90 MxB KO cells were constructed by transducing the cells with lentiviral vector (lentiCRISPR v2; Addgene #52961) containing a gRNA targeting the genomic MxB target sequence (5’-GGTGGTGGTTCCCTGTAACG-3’ ...
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bioRxiv - Cell Biology 2023Quote: ... untransfected HEK293T cells or cells transfected 48 hours earlier with pEGFP-C1 Lifeact-EGFP (Addgene, plasmid #58470) were treated with Wnt5a CM or protein (200 ng/ml ...
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bioRxiv - Cancer Biology 2023Quote: ... MDA-MB-231 cells and NCI-H1299 cells were infected with pLentiCRISPR V2 viral vector (Addgene, # 52961) in which the sgRNA for human MPC1 gene (5’-AAGTCTCCAGAGATTATCAG-3’ ...
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bioRxiv - Microbiology 2021Quote: Stable ACE2 expressing HeLa cells (HeLa-ACE2) were generated by transducing HeLa-dCas cells with pLENTI_hACE2_HygR (Addgene, #155296) followed selection with hygromycin ...
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bioRxiv - Cancer Biology 2020Quote: ... LNCaP cells stably expressing dCas9-KRAB were generated by transducing LNCaP cells with Lenti-dCas9-KRAB (Addgene #89567) followed by blasticidin selection ...
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bioRxiv - Molecular Biology 2019Quote: Inducible Cas9 (iCas9) BL cells were established by transducing cells with pCW-Cas9-Blast-based lentiviruses (Addgene #83481) and selecting with Blasticidin ...