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Citations for Addgene :
1 - 45 of 45 citations for Anti PKC mu since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... Mu-BsaI transposon was digested from pUC-KanR-Mu-BsaI (Addgene plasmid # 79769) with BglII and HindIII in Buffer 3.1 (NEB ...
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bioRxiv - Cancer Biology 2022Quote: ... The constitutively active PKC mutant expression plasmid (PKC alpha CAT) was a gift from Bernard Weinstein (Addgene plasmid # 21234 ...
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bioRxiv - Cancer Biology 2022Quote: ... The constitutively active PKC mutant expression plasmid (PKC alpha CAT) was a gift from Bernard Weinstein (Addgene plasmid # 21234; http://n2t.net/Addgene:21234; RRID: Addgene_21234). YAP/TAZ-responsive synthetic promoter (8xGTIIC-luciferase ...
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bioRxiv - Cancer Biology 2019Quote: ... which was a gift from David Mu (Addgene plasmid #75085 ...
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bioRxiv - Cancer Biology 2024Quote: ... pMSCV-Blasticidin was a gift from David Mu (Addgene #75085)66 ...
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bioRxiv - Molecular Biology 2022Quote: ... PKC sensor pcDNA3-ExRai-CKAR was a gift from Jin Zhang (Addgene plasmid # 118409 ...
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bioRxiv - Biochemistry 2021Quote: ... PKC plasmids (PKCα, β, γ, δ, ε and θ) were obtained from Addgene, and their coding sequences were subcloned into the pcDNA3.1 vector containing the Flag epitope tag ...
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bioRxiv - Cancer Biology 2019Quote: ... which was a gift from David Mu (Addgene plasmid #75085; http://n2t.net/addgene:75085; RRID:Addgene_75085). The second set of transfections was with an empty backbone plasmid ...
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bioRxiv - Cancer Biology 2022Quote: ... The constitutively active PKC mutant expression plasmid (PKC alpha CAT) was a gift from Bernard Weinstein (Addgene plasmid # 21234; http://n2t.net/Addgene:21234; RRID: Addgene_21234). YAP/TAZ-responsive synthetic promoter (8xGTIIC-luciferase ...
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bioRxiv - Cell Biology 2020Quote: For the generation of stable A549 cell lines expressing various PKCs and mutants we used following plasmids: pcDNA3-PKCε-Flag (wild-type, Addgene, Cambridge, MA), pcDNA3-Flag-K312PKCε ...
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bioRxiv - Cell Biology 2021Quote: ... anti-HA FB-SNAP and anti-HA FB-Halo (Addgene #129592), cut by EcoRI and combined with anti-FLAG frankenbody gblocks by Gibson assembly ...
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bioRxiv - Bioengineering 2022Quote: ... Anti-CD19 and anti-Spike scFv amino acid sequences were obtained from Addgene plasmids #79125 and #155364 ...
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bioRxiv - Bioengineering 2022Quote: ... Anti-CD19 and anti-HER2 scFv amino acid sequences were obtained from Addgene plasmids #79125 and #85424 ...
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bioRxiv - Immunology 2022Quote: ... Anti-CD19 and anti-Her2 scFv amino acid sequences were obtained from Addgene plasmids #79125 and #85424 ...
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bioRxiv - Cancer Biology 2021Quote: ... pLVX-anti-MIR211-5p (Addgene #153318), pLVX-anti-MIR328-3p (Addgene #153319) ...
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bioRxiv - Cancer Biology 2021Quote: ... pLVX-anti-MIR328-3p (Addgene #153319), and pLVX-Che-zsGreen (Addgene #153320 ...
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bioRxiv - Molecular Biology 2021Quote: ... The expression vector anti-RBD Sybody (Addgene #153522) was a kind gift from Prof ...
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bioRxiv - Genetics 2023Quote: ... anti-Na/K-ATPase (1:1000, Addgene #180089) (membrane protein loading control) ...
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bioRxiv - Biochemistry 2023Quote: ... and inserted into two model DARPins: 1) 3G124 (anti-GFP) and 2) and Off7 (anti-MBP) and cloned into a pET vector (Addgene plasmid #29666) containing a N-terminal His-Tag ...
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bioRxiv - Synthetic Biology 2020Quote: ... Expression vectors encoding Anti-CRISPR proteins were obtained from Addgene: AcrIIA2 (pJH373 plasmid ...
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bioRxiv - Genetics 2021Quote: CRISPR-Cas and anti-CRISPR plasmids were ordered from Addgene, thanks to gifts from many investigators (Hou et al ...
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bioRxiv - Cell Biology 2023Quote: ... Then His14-Avi-SUMOEu1-tagged anti-GFP nanobody (Addgene #149336) was immobilized onto the washed magnetic beads for 30 min with mixing at 4°C using a ratio of 27 μg for every 80 μl beads ...
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bioRxiv - Biochemistry 2022Quote: ... CRISPR sense and anti-sense guides were cloned into pX335 (Addgene plasmid 42335 ...
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bioRxiv - Cell Biology 2023Quote: ... Anti-mCherry GST-tagged nanobodies (Addgene #70696, (Katoh et al, 2016)) were expressed and purified according to published protocol (Katoh et al. ...
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bioRxiv - Biochemistry 2021Quote: ... coli cells (BL21DE3) expressing GST tagged anti-GFP nanobody (Addgene plasmid #61838) (Katoh et al. ...
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bioRxiv - Biochemistry 2022Quote: ... purified His14-Avi-SUMOEu1-anti GFP nanobody (expressed from pTP396, Addgene #149336)74 was biotinylated using BirA (expressed from pTP264 ...
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bioRxiv - Cell Biology 2021Quote: ... The scFv gblocks were ligated with linearized anti-HA frankenbody-mEGFP (Addgene # 129590) by EcoRI through Gibson assembly (anti-FLAG FB-mEGFP) ...
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bioRxiv - Cell Biology 2022Quote: ... encoding anti-GFP HALO nanobody (a gift from Lennart Wirthmueller, Addgene plasmid #111090) were expressed in bacteria and respectively GST- and His-purified in-house ...
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bioRxiv - Molecular Biology 2020Quote: ... A plasmid expressing anti-CRISPR protein (pEJS581; pCSDest2-AcrIIC4Hpa-FLAG-NLS; Addgene # 113436) was included in the triple-transfection packaging process to maintain intact rAAV:HDR:cleaved plasmids during production ...
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bioRxiv - Molecular Biology 2020Quote: The expression of His14-Avi-SUMOEu1-anti GFP nanobody from plasmid pTP396 (Addgene # 149336) was carried out exactly as described in detail before (Pleiner et al. ...
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bioRxiv - Cell Biology 2022Quote: ... The expression of His14-Avi-SUMOEu1-anti GFP nanobody from plasmid pTP396 (Addgene #149336) was carried out with the following modification ...
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bioRxiv - Molecular Biology 2021Quote: The bacterial expression vector for anti-GFP nanobody pGEX-6P-1 was procured from Addgene. E ...
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bioRxiv - Cell Biology 2021Quote: ... the GST-tagged anti-GFP nanobody construct in pGEX-6P-1 vector (Addgene ID # 61838) was transformed to BL21 RIL (DE3 ...
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bioRxiv - Biophysics 2023Quote: Anti-EGFP nanobody plasmid (pOPINE GFP) was a gift from Brett Collins (Addgene plasmid #49172)43 and transformed into BL21 cells for purification ...
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bioRxiv - Cell Biology 2022Quote: ... The prokaryote expression vector encoding an anti-GFP mCherry nanobody (a gift from Martin Spiess, Addgene plasmid #109421) and pOPINE GFP nanobody:HALO:His6 ...
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bioRxiv - Synthetic Biology 2023Quote: A lentiviral transfer plasmid encoding encoding an anti-CD19 synNotch receptor driven by pPGK was acquired from Addgene (pHR_PGK_antiCD19_synNotch_Gal4VP64 was a gift from Wendell Lim ...
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bioRxiv - Biochemistry 2020Quote: ... anti-mouse IgG AlexaFluor-488 (Life Technologies A11029-EA. The following plasmids were used: mouse PM20D1-flag (Addgene 84566). pENN.AAV.tMCK.PI.eGFP.WPRE.bGH (Addgene 105556) ...
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bioRxiv - Molecular Biology 2021Quote: The Mir20b and anti-Mir20b were cloned into the pOTTC385-pAAV CMV-IE IRES EGFP vector (Addgene plasmid # 102936)(Nelson et al. ...
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bioRxiv - Cell Biology 2024Quote: ... anti-ALFA nanobody fragment was amplified using primers s15 and s16 (Table 3) from its expression vector (Addgene #189755). A pET24a-VHH-std vector (Addgene #109417 ...
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bioRxiv - Cell Biology 2020Quote: The anti-GCN4-scFv was generated from pHR-scFv-GCN4-sfGFP-GB1-dWPRE (Addgene plasmid 60907 (Tanenbaum et al., 2014), cut with EcoRI/XbaI and inserted into pcDNA3 ...
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bioRxiv - Bioengineering 2024Quote: ... pET28a-SnoopTag-AffiHER2-SpyTag (N-terminal His6–SnoopTag–anti-HER2 Affibody–SpyTag) (GenBank accession no. KU296975) (Addgene deposition in progress) (Brune et al. ...
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bioRxiv - Synthetic Biology 2020Quote: ... DNA encoding the SNAP or mSA2 coding region was codon-optimized and synthesized (Integrated DNA Technologies) and cloned in place of the anti-CD19scFv in plasmid pHR-PGK-antiCD19-synNotch-Gal4VP64 (Addgene# 79125) using isothermal assembly ...
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bioRxiv - Molecular Biology 2020Quote: ... Expression constructs needed to generate biotinylated anti-GFP nanobody for native purification from human cells are available from Addgene (#149336, #149334, #149333) (Pleiner et al. ...
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bioRxiv - Cell Biology 2023Quote: MDCK cells expressing GFP-Rab19 on the Rab19 KO background were lysed and incubated with GST-tagged anti-GFP nanobody (recombinantly produced from pGEX6P1-GFP-Nanobody, Addgene Plasmid #61838) or with free GST for negative control ...
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bioRxiv - Genetics 2024Quote: To generate the B16F10 Capza3 KO lines the 2 CRISPR gRNAs enriched in the radiation alone and radiation and anti-PD1 antibody screen treatment conditions were cloned into the LRG vector (Addgene Plasmid # 65656). For WT controls a NTC gRNA from the CRISPR screen was cloned into the LRG vector ...