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Citations for Addgene :
1 - 50 of 122 citations for 2x SYBR Green qPCR Master Mix Low ROX since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... and a separate master mix was prepared comprising 1 mL Opti-MEM 8 µg PSPAX (12260, Addgene, Watertown, MA), 2 µg PMD2G plasmids (12259 ...
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bioRxiv - Neuroscience 2022Quote: ... Viral vectors were generated from Addgene plasmids #20297 and #50459 and titers determined via fluorometric quantification (Neuroscience Center Zurich) or by real-time quantitative PCR combined with SYBR green technology (Addgene). Respective titers were >9.1×1012 vector genomes/ml (Neuroscience Center Zurich ...
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bioRxiv - Neuroscience 2023Quote: ... AAVrg-hSyn-eGFP (RRID:Addgene_50465, 300 nl at 2x 1013 GC/ml) were injected into the pontine nuclei to regrogradely label PT neuron in the motor cortex ...
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bioRxiv - Neuroscience 2023Quote: ... consisting of a loxP site and rox-flanked hygromycin and zeocin resistance genes from pNeDaKO-Hyg (Addgene plasmid #16414; (60)) ...
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bioRxiv - Developmental Biology 2021Quote: ... The NLS 2x MCP from pHsp83-NLS-HA-2xMCP-2xTagRFP-T (Addgene 71242) (Halstead et al. ...
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bioRxiv - Cell Biology 2023Quote: ... but using pLV-dimericTomato-2x-rGBD (a gift from Dorus Gadella; Addgene #176098) as transfer plasmid in HEK293T cells ...
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bioRxiv - Neuroscience 2019Quote: Green CEPIA1er cDNA was purchased from Addgene. Imaging of ER calcium levels using G-CEPIA1er was performed in the same manner as fura-2 imaging however single-wavelength excitation at 488nm was used.
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bioRxiv - Genetics 2020Quote: ... low copy Tdp-43 expression plasmid pRS416-GAL1p-TDP43-YFP (URA3) (Addgene: 27447), high copy Tdp-43 expression plasmid pRS426-GAL1p-TDP43-GFP (URA3 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Inserts were cloned into a low copy pACYC184-derived empty vector (Addgene # 157879) between the HindIII and EcoRI restriction sites using NEBuilder HiFi DNA Assembly mix (New England Biolabs) ...
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bioRxiv - Plant Biology 2024Quote: ... the low copy number pFA31 backbone (Addgene plasmid #162708; Flamholz et al., 2020) was modified into two terminal acceptors compatible with Loop Assembly (pFA-Odd and pFA-Even) ...
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bioRxiv - Cancer Biology 2023Quote: ... Transfection mix consisting 47.6μg pMD2G (Addgene), 95.2μg of psPAX2 (Addgene) ...
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bioRxiv - Cell Biology 2020Quote: ... We then excised 2xmCherry from pDZ585 pKAN 2x-mCherry (Saroufim et al., 2015) (Addgene # 72236) and blunt cloned the cassette in frame in place of 2xYFP.
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bioRxiv - Neuroscience 2020Quote: ... rgAAV-hSyn-DIO-EGFP (retrograte green; Addgene 50457), rgAAV-CAG-FLEX-tdTomato (retrograde red ...
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bioRxiv - Neuroscience 2022Quote: ... The coding sequence for CreM was obtained from Addgene (Kaczmarczyk and Green JE, 2001; Addgene plasmid #8395). These constructs were used in Figures 1 and 2 and associated supporting figures ...
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bioRxiv - Genetics 2023Quote: ... The coding sequence for CreM was obtained from Addgene (Kaczmarczyk and Green JE, 2001; Addgene plasmid #8395). FlpOM was generated by introducing the human b-globin intron from CreM into a codon optimized Flp (Raymond and Soriano ...
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bioRxiv - Developmental Biology 2022Quote: ... A mix of plasmids (all from Addgene) including pCXLE-hOCT3/4-shp53-f (27077) ...
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bioRxiv - Neuroscience 2022Quote: ... Viral titers were determined using a qPCR method provided by Addgene. The typical viral titers were ∼ 1014 genome copies per mL.
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bioRxiv - Plant Biology 2019Quote: ... the green seed coat vector pFG7m34GW (Addgene ID: 133747). It was generated by cloning the FastGreen module into the pP7m34GW vector13 ...
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bioRxiv - Molecular Biology 2022Quote: The packaging mix consisting of psPAX2 (Addgene #12260) and pMD2.G (Addgene #12259 ...
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bioRxiv - Neuroscience 2022Quote: ... [14] and low-titer Cre (AAV9-hSyn-Cre-WPRE-hGH; Addgene #105553, MOI = 5×103 vg) AAVs on DIV 7 ...
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bioRxiv - Developmental Biology 2020Quote: ... The 3x FLAG 2x Strep tag sequence was amplified from AAVS1 Puro Tet3G 3xFLAG Twin Strep (Addgene, # 92099) (Dalvai et al. ...
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bioRxiv - Synthetic Biology 2021Quote: ... a green florescence protein (GFP) derived from pJL1 (Addgene #69496), and a C-terminal twin-strep-tag (66) ...
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bioRxiv - Biochemistry 2021Quote: ... or N-terminal His6 plus green fluorescent protein (GFP; RRID:Addgene_29716). Note that in the plasmid names for this clone the numbering of Syx residues was based on the Syx isoform from NCBI Reference Sequence NP_001036128.1 ...
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bioRxiv - Cell Biology 2023Quote: ... and enhanced green fluorescent protein (eGFP) (Addgene, Boston, MA, USA). Cells were nucleofected using the Amaxa 4D-Nucleofector (Lonza ...
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bioRxiv - Neuroscience 2024Quote: ... Wild-type animals were then injected whether with mix 1 or mix 2 or AAV9-CamKII-GCaMP6f-WPRE-SV40 (Addgene, 100834, vg/mL) or AAV9-CamKII-hM4D(Gi)-mCherry (construct provided by Bryan Roth ...
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bioRxiv - Biochemistry 2023Quote: Mass spectrometry sample preparation: N2A cells were transfected with pcDNA3 plasmids containing 2X myc-EXOSC3 or pcDNA3 empty vector (Addgene) using Lipofectamine® 2000 (Thermofisher ...
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bioRxiv - Neuroscience 2020Quote: ... a mix of pAAV hSyn-Flex RG-cerulean (Addgene 98221) and pAAV-EF1a-FLEX-GT (gift from Edward Callaway ...
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bioRxiv - Neuroscience 2023Quote: ... method into pDONR221 entry vector and then recombined into a yeast low-copy number CEN destination vector (Addgene, 70) to obtain pAG413-promGPD-WDR47 (pSF634) ...
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bioRxiv - Biophysics 2019Quote: ... pGFP-Cytochrome C was a gift from Douglas Green (Addgene plasmid # 41181). GPI-mNeonGreen was created by replacing GFP with mNeonGreen ...
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bioRxiv - Cell Biology 2023Quote: ... we used the following emerald-green GFP (emGFP)-tagged vectors from Addgene: BAZ1A (#65371) ...
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bioRxiv - Neuroscience 2024Quote: Enhanced green fluorescent protein (EGFP) plasmid (pEGFP-C1) was commercially purchased (Addgene). The fluorescent protein mKate was inserted into a pcDNA3.1(- ...
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bioRxiv - Synthetic Biology 2019Quote: A plasmid for SpCas9 expression (2x NLS and C-terminal His tag, pET-28a) was a gift from the Gao group (Addgene #98158).50 E ...
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bioRxiv - Genetics 2022Quote: ... 2015) flanked by 2X core sequence of the HS4 chicken beta globin insulator was cloned into a targeting vector (Addgene #92142) that contains homology arms of the mouse TIGRE genomic locus (Madisen et al. ...
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bioRxiv - Cell Biology 2021Quote: ... and sequences with low off-target score were synthetised as oligonucleotides and cloned into a PX458 plasmid (Addgene plasmid #48138). gRNA sequences used can be found in Fig ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were infected at low viral titer with the pooled mouse CRISPR lentiviral library containing 78,637 gRNAs targeting 19,674 genes (Addgene #73633-LV). Infected cells were selected with puromycin (1 μg/ml ...
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bioRxiv - Cell Biology 2020Quote: Identified gRNAs in organoids which survived under low Wnt conditions were cloned into pX330-U6-Chimeric_BB-CBh-hSpCas9 (a gift from Feng Zhang, Addgene #42230) and transfected into organoids and HEK293T cells with Lipofectamine LTX or Lipofectamine 2000 (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: Cas9-expressing HAP1 cells were generated by low multiplicity of infection transduction with lentivirus generated from lentiCas9-Blast74 (Addgene 52962) as described in the preceding paragraph ...
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bioRxiv - Cell Biology 2023Quote: ... RAD52KO and RAD52WT cells were transduced at a low multiplicity of infection (MOI<0.3) using the BFP-tagged Human Improved Genome-wide Knockout CRIPSR Library (Addgene #67989) (Tzelepis et al ...
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bioRxiv - Neuroscience 2022Quote: ... Synaptophysin-pHluorin (syn-pH) was a gift from Stephen Heinemann and Yongling Zhu (Northwestern University, Evanston, IL; pcDNA3-SypHluorin 2x, Addgene plasmid # 37004). VAMP-mCherry and synaptophysin-GCaMP6f were gifts from Timothy Ryan (Weill Cornell Medicine ...
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bioRxiv - Bioengineering 2023Quote: Plasmids pab202 and pab633 (gifts from Feng Zhang lab) and pAAV-CMV-dSa-VPR mini.-2X sNRP1 (a gift from George Church; Addgene plasmid # 99688) were used ...
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bioRxiv - Plant Biology 2019Quote: ... Green fluorescent protein (GFP) from the Gateway entry vector pENTR4-GFP-C1 (Addgene) was transferred to pYES-DEST52 and pAG426GAL-ccdb using Gateway LR Clonase ...
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bioRxiv - Neuroscience 2020Quote: ... Retrograde adeno associated viruses encoding green fluorescent protein (Addgene, Catalog number 50465-AAVrg) or tdtomato (Addgene ...
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bioRxiv - Cancer Biology 2023Quote: ... cDNAs were subcloned into the pMSCV-IRES-green fluorescence protein vector (Addgene #20672) with reference to a report by Masubuchi et al.43 The constructed plasmid was validated by sequencing prior to use.
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bioRxiv - Bioengineering 2021Quote: ... Plasmids mix for each transfection consisted of psPax2 (packaging; Addgene plasmid # 12260), pCMV-SCoV-2S (Spike envelope plasmid ...
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bioRxiv - Neuroscience 2020Quote: ... a mix of pAAV-EF1a cre off Cer-E2A-RG (Addgene 126471) and pAAV-EF1a-cre off EGFP-2A-TVA (Addgene 126470 ...
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bioRxiv - Developmental Biology 2020Quote: ... The injection mix contained: 60 ng/µl Peft-3::Cas9 (Addgene #46168), 15 ng/µl repair template ...
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bioRxiv - Cell Biology 2023Quote: ... with 0.75 μg third-generation lentiviral packaging mix (pMD2.G (Addgene #12259), pRSV-Rev (Addgene #12253) ...
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Pathogenic variants of sphingomyelin synthase SMS2 disrupt lipid landscapes in the secretory pathwaybioRxiv - Cell Biology 2022Quote: ... low passage HEK293T cells were co-transfected with the corresponding pInducer20-FLAG-SMS2 construct and the packaging vectors psPAX2 (Addgene, 12260) and pMD2.G (Addgene ...
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bioRxiv - Genetics 2020Quote: ... were transduced at a low multiplicity of infection (MOI) with virus containing sgRNA library cloned in lentiGuide-Puro (Addgene plasmid 52963) to ensure that most cells received only one sgRNA in expansion phase medium42 ...
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bioRxiv - Immunology 2021Quote: ... green fluorescence protein (GFP) or an enhanced yellow fluorescent protein (EYFP) sequence (pcDNA1.3-, Addgene) using standard cloning methods ...