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Citations for Addgene :
151 - 200 of 823 citations for SARS Coronavirus Spike Glycoprotein S1 Mosaic N Term E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... gRNA expression vectors U6.3> gRNA.f+e and U6.3> Control.gRNA.f+e were obtained from Addgene (Gandhi et al., 2017). Oligo DNAs for the gRNA templates were designed as follows (lowercase letters indicate BsaI overhang) ...
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bioRxiv - Developmental Biology 2019Quote: ... CrispR guides were made by cloning annealed oligos (Table S1) into px458 (Addgene). 2μg of vector DNA were transfected into 8×105 ESCs using Lipofectamine 2000 (ThermoFisher ...
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bioRxiv - Synthetic Biology 2024Quote: ... carrying a previously constructed helper plasmid (24) (pDY118A, Figure S1, Addgene ID 182958). pDY118A encodes SsrA-tagged SpCas9 (10 ...
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bioRxiv - Cancer Biology 2021Quote: ... N-Myc (Addgene #74163) was cloned into plasmid pcDNA5/FRT/TO (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... coli C321.ΔprfA EXP11 (Addgene #48998). For stop codon suppression by genetic code expansion ...
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bioRxiv - Biophysics 2021Quote: ... coli from pet30b-7M SrtA (Addgene plasmid # 51141 was a gift from Hidde Ploegh ...
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bioRxiv - Microbiology 2021Quote: ... coli MG1655 (delta-Z, Addgene: #52706) harboring the empty-BAC ...
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bioRxiv - Systems Biology 2024Quote: ... coli plasmid-propagating strains from Addgene, and are a generous gift from the Roth laboratory by way of Addgene [69] ...
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bioRxiv - Synthetic Biology 2023Quote: ... coli MG1655 K12 cells (Addgene #37854) transformed with reporter ...
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bioRxiv - Cell Biology 2023Quote: ... coli strains producing plasmids pEF.CMV.RFP (Addgene #17619 ...
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bioRxiv - Molecular Biology 2021Quote: ... pcDNA3.1-SARS2-Spike was a gift from Fang Li (Addgene plasmid #145032) (76) ...
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bioRxiv - Neuroscience 2021Quote: ... The pcDNA3.1-SARS2-Spike plasmid was a gift from Fang Li (Addgene plasmid # 145032 ...
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bioRxiv - Immunology 2021Quote: Plasmid encoding spike HexaPro (designated “S” throughout) was procured from Addgene (#154754) and transfected into Expi293F™ cells (ThermoFisher Scientific ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The pcDNA3.1-SARS2-Spike (a gift from Fang Li; Addgene plasmid #145032),19 ...
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bioRxiv - Cancer Biology 2021Quote: ... The sgRNA pair targeting the IKZF3 gene locus was designed using E-CRISP website (http://www.e-crisp.org/E-CRISP/) and expressed from pGL3-U6 sgRNA-PGK-Puro vector (Addgene 51133).
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bioRxiv - Cell Biology 2020Quote: ... The construct pT2-CAG-GFP-E-Syt1 was generated by restriction digestion of the EGFP-E-Syt1 (Addgene #66830) and the pT2-CAG-fGFP plasmids with AgeI and MluI ...
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bioRxiv - Cell Biology 2023Quote: ... Primary MEFs used for the long term cell migration and JLY experiments were infected with viral media from HEK cells transfected with 3x-mScarlet-FTractin (Addgene Plasmid #112960)(53) ...
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bioRxiv - Bioengineering 2020Quote: ... and pcDNA3-SARS-CoV-2-S-RBD-Fc (Addgene Plasmid #141183) were obtained as gifts from Erik Procko ...
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bioRxiv - Molecular Biology 2021Quote: SARS-CoV-2 viral proteins were amplified via PCR from Addgene constructs with a 1x (GGGS ...
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bioRxiv - Biochemistry 2021Quote: ... and SARS-CoV-2 nsp14/nsp10-His-3xFlag (Addgene ID 169164) were expressed in baculovirus-infected Sf9 insect cells ...
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bioRxiv - Biochemistry 2021Quote: ... we co-transformed plasmids SARS-CoV-2 nsp10 (Addgene ID 169158) and SARS-CoV-2 3xFlag-nsp5CS-nsp14 (Addgene ID 169159) ...
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bioRxiv - Biophysics 2021Quote: ... the pet28 plasmid containing His6 SARS-CoV-2 nsp13 (Addgene #159390) was transformed into E ...
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bioRxiv - Microbiology 2021Quote: ... Expression vectors for SARS-CoV-2 Wuhan-Hu-1 (Addgene, #149539), SARS-CoV-2 B.1.167.2 (Addgene ...
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bioRxiv - Molecular Biology 2024Quote: ... and pDONR207 SARS-CoV-2 nsp1 R124A/K125A (M2, Addgene #164523) plasmids using the primers 5-EX-NSP1 and 3-NB-NSP1 and cloned into pEGFP-N1 digested with EcoRI and NotI enzymes ...
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bioRxiv - Neuroscience 2023Quote: ... Neuro2a cells stably expressing the CVS-N2c glycoprotein (N2A-N2cG_02 cells) were transfected with the barcoded N2c library along with helper plasmids pCAG-N2cN (Addgene cat. # 100801), pCAG N2cP (Addgene cat ...
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bioRxiv - Neuroscience 2023Quote: ... AAV9-hSyn- GrabDA1h (n = 5 Area X hemispheres; n = 2 MST hemispheres; Addgene), AAV5-CAG- Dlight1.1 (n = 1 Area X hemisphere ...
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bioRxiv - Developmental Biology 2022Quote: ... The guide sequence (r4g9, Table S1) was cloned into pX459 vector from Addgene (#62988) and 8ug of the vector was used for mESC transfection ...
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bioRxiv - Genomics 2023Quote: ... oligos containing the CRISPR sequence (table S1) were inserted into pWS082 (Addgene plasmid #90516), the template plasmid of sgRNA cassette ...
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bioRxiv - Microbiology 2023Quote: ... The CoV2-M-IRES-E (Addgene plasmid # 177938), Luc-T20 (Addgene plasmid # 177941 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... membrane and envelope (CoV2-M-IRES-E, Addgene), and spike (nCoV-2-B117 or B117/M1237I ...
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bioRxiv - Bioengineering 2022Quote: ... Anti-CD19 and anti-Spike scFv amino acid sequences were obtained from Addgene plasmids #79125 and #155364 ...
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bioRxiv - Molecular Biology 2022Quote: ... pcDNA3.1-SARS2-Spike was a gift from Dr Fang Li (Addgene plasmid # 145032); pcDNA3.1-ACE2-GFP was a gift from Dr Utpal Pajvani (Addgene plasmid # 154962) ...
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bioRxiv - Microbiology 2020Quote: ... and pcDNA3.1 expressing different spike genes or VSV-G (pMD2.G (Addgene #12259)) using Fugene®HD tranfection reagent (Promega) ...
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bioRxiv - Molecular Biology 2021Quote: ... N-terminal FKBP12F36V fragments were originally amplified originally from pLEX_305-N-dTAG (Addgene #91797) (Nabet et al. ...
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bioRxiv - Pathology 2020Quote: ... Lentiviral vector pLV-mCherry and vesicular stomatitis virus glycoprotein (VSV-G) expression vector pMD2.G were obtained from Addgene (Watertown, MA, USA). Coding sequence of SARS-CoV-2 S gene (GenBank ...
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bioRxiv - Biochemistry 2022Quote: ... coli expression vector was purchased from Addgene. The pEGFP-C3 vector ...
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bioRxiv - Molecular Biology 2019Quote: ... coli using pFGET19_Ulp1 plasmid purchased from Addgene. The gBlock (gBlocks® Gene Fragments ...
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bioRxiv - Biochemistry 2021Quote: ... coli expression vector was purchased from Addgene. The pUCPT20 encoding GFP for constitutive gene expression in P ...
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bioRxiv - Genetics 2021Quote: ... coli bacteria expressing either L4440 (Addgene, #1654) empty vector control or L4440 carrying inserts to trigger the production of dsRNA (RNAi ...
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bioRxiv - Bioengineering 2021Quote: We cloned the TRS-Leader-SARS-CoV-2-d2eGFP plasmid (Addgene 171585) by introducing 75 nt of the SARS-CoV-2 Leader sequence into the NheI-digested EFS-EGFPd2PEST-2A-Hygro plasmid (Addgene 138152) ...
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bioRxiv - Biochemistry 2021Quote: The plasmid SARS-CoV-2 3xFlag-His-nsp5CS-nsp15 (Addgene ID: 169166) was used to express 3xFlag-His-nsp5CS-nsp15 in baculovirus-infected insect cells (Supplementary Table S1 and S2) ...
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bioRxiv - Genetics 2021Quote: ... pLVX-EF1alpha-SARS-CoV-2-orf8-2xStrep-IRES-Puro (Addgene plasmid #141390). Orf8 deletion constructs were produced on the Orf8 backbone using Pfu Turbo HotStart DNA polymerase (Agilent 600322-51 ...
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bioRxiv - Molecular Biology 2021Quote: pLVX-EF1alpha-SARS-CoV-2-Nsp1-2XStrep-IRES-Puro plasmid (Addgene, 141367) and pLVX-EF1alpha-SARS-CoV-2-Nsp2-2XStrep-IRES-Puro plasmid (Addgene ...
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bioRxiv - Molecular Biology 2023Quote: pDONR207 SARS-CoV-2 NSP1 was a gift from Fritz Roth (Addgene plasmid # 141255 ...
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bioRxiv - Immunology 2023Quote: ... Plasmids expressing the SARS-CoV-2 S protein were obtained from Addgene: pcDNA3.3-SARS2-B.1.617.2 (Delta ...
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bioRxiv - Molecular Biology 2024Quote: ... pDONR207 SARS-CoV-2 nsp1 Delta RC (M1, K164A/H165A, Addgene #164522) and pDONR207 SARS-CoV-2 nsp1 R124A/K125A (M2 ...
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bioRxiv - Biochemistry 2022Quote: ... Expression constructs for various cytoskeleton or organelle marker proteins were obtained from Addgene (S1 Table). For bacterial expression of His6-tagged CAHS proteins ...
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bioRxiv - Developmental Biology 2021Quote: sgRNA and shRNA oligonucleotides (Table S1) were cloned into lentiCRISPR v2 (Addgene plasmid no. #52961) and into pSMP vector (Addgene plasmid no ...
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bioRxiv - Cell Biology 2024Quote: ... pcDNA3-N-HA-NEK7 and pcDNA3-N-HA-NEK7K64M were gifts from Bruce Beutler (Addgene plasmid # 75142 ...
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bioRxiv - Molecular Biology 2023Quote: ... are a derivative of HEK293T-E with a second-generation lentiviral vector generated with the pCW57-E-IRES-ORF6 (Addgene plasmid #80921) as a transfer vector ...