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Citations for Addgene :
151 - 200 of 1157 citations for Recombinant Human S100A3 His & MBP tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2019Quote: ... recombinant mouse E1 (Addgene plasmid # 32534), E2 (pGEX4T-1-UbcH5b) ...
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bioRxiv - Neuroscience 2020Quote: MBP-FUS was a gift from Nicolas Fawzi (Addgene plasmid # 98651; http://n2t.net/addgene:98651; RRID:Addgene_98651). The P525L FUS point mutation and the Δ14 mutant version of FUS were generated via site-directed mutagenesis using MBP-FUS ...
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bioRxiv - Biophysics 2021Quote: ... The pET MBP His6 LIC cloning vector (2Cc-T) was a gift from Scott Gradia (Addgene plasmid # 37237 ...
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bioRxiv - Cell Biology 2022Quote: ... Constructs encoding the DFCP1 N-terminus were cloned into pET-His6-MBP-TEV-LIC (Addgene # 29708) and Rosetta2(DE3 ...
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bioRxiv - Biophysics 2021Quote: ... coli expression vector with an N-terminal 6xHis tag followed by an MBP tag (Addgene #29654) by ligation independent cloning (LIC) ...
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bioRxiv - Cell Biology 2024Quote: ... NTF2 domain of G3BP1 variants was cloned into a pET28 MBP-TEV bacterial backbone (Addgene, 69929) with Gibson assembly.
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bioRxiv - Cell Biology 2023Quote: ... we expressed the subcomplex FIP200-ATG13-ATG101 from pCAG vectors encoding GST-TEV-FIP200-MBP (RRID:Addgene_171410), ATG13 (RRID:Addgene_171412) ...
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bioRxiv - Cell Biology 2023Quote: ... EGFP-MISP sequence was subcloned into modified pFastBac-6xHis-MBP LIC expression vector (Addgene; plasmid #30116). In-frame sequence insertion was confirmed by sequencing ...
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bioRxiv - Biochemistry 2023Quote: Two vectors with C-terminal MBP (maltose-binding protein)-His6 Tag (2Cc-T; Addgene Plasmid #55209) or N-terminal His10-MBP Tag (2CT-10 ...
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bioRxiv - Cell Biology 2021Quote: GFP-tagged galectin 3 (pEGFP-hGal3 (Addgene, plasmid no. 73080) was mutated using the Q5 site directed mutagenesis kit (New England Biolabs ...
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bioRxiv - Genetics 2019Quote: FLAG tagged ubiquitin was cloned in pCW57.1 vector (Addgene # 41393) between BsrG1 sites and verified through sequencing ...
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bioRxiv - Cell Biology 2022Quote: ... T7-tagged p65 WT and mutants were purchased from Addgene: T7-RelA (#21984) ...
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bioRxiv - Cancer Biology 2023Quote: ... HA-tagged SOX4 [43] or empty vector control (Addgene 12257). SMARCA4 was overexpressed by WT SMARCA4-sfGFP (Addgene 107056 ...
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bioRxiv - Cell Biology 2023Quote: GFP tagged WT Keratin 8 in pEGFP-N3 (Addgene 6080) was kindly provided by Dr ...
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bioRxiv - Cell Biology 2023Quote: ... mCherry-tagged CLIMP63 (gift from Gia Voeltz 52, Addgene, 136293),mcherry-FIS1 (gift from Uri Manor53) ...
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bioRxiv - Molecular Biology 2023Quote: ... C-terminally AcGFP-tagged SOD1 variants SOD1-WT (Addgene: #264074) and SOD1-G85R (Addgene ...
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bioRxiv - Biochemistry 2024Quote: His6-tagged MSP1D1 and MSPE3D1 (Addgene plasmids #20061 and #20066) were expressed in E ...
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bioRxiv - Cell Biology 2023Quote: ... Then His14-Avi-SUMOEu1-tagged anti-GFP nanobody (Addgene #149336) was immobilized onto the washed magnetic beads for 30 min with mixing at 4°C using a ratio of 27 μg for every 80 μl beads ...
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bioRxiv - Developmental Biology 2021Quote: ... the pET-28b-RfxCas13d-His vector (Addgene, Plasmid #141322) was used for RfxCas13d protein production (Bon Opus Biosciences ...
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bioRxiv - Biochemistry 2020Quote: ... The FLAG-His sequence in payload plasmid pKM491 (Addgene) was replaced with sequence for a 3×FLAG tag by Gibson Assembly (New England Biolabs ...
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bioRxiv - Biochemistry 2020Quote: ... insoluble His-tag purification (Addgene ID: 139126, 139127, 139128)
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bioRxiv - Biochemistry 2020Quote: ... soluble His-tag purification (Addgene ID: 139114, 139115 respectively)
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bioRxiv - Cell Biology 2024Quote: ... pET-His-GST-tev-LIC (Addgene #29655, Gradia Lab), pGEX-4T-3-mR7BD (Addgene #79149 ...
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bioRxiv - Neuroscience 2022Quote: The pJ4M/TDP-43 vector encoding 6xHis-TDP-43-TEV-MBP was obtained from Addgene (#104480; 33). A His-tagged proSAAS cDNA (lacking the signal peptide ...
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bioRxiv - Biochemistry 2021Quote: ... GST-tag and MBP-tag were attached to its N- and C-terminus respectively (Addgene ID 169195). The cleavage at G//A resulted in 25 and 42 kDa products ...
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bioRxiv - Biochemistry 2021Quote: ... pET 6His MBP TEV LIC cloning vector (1M) was a gift from Scott Gradia (Addgene plasmid # 29656).
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bioRxiv - Microbiology 2021Quote: ... marinum genomic DNA and cloned in-frame into the SspI site of 6XHis-MBP-TEV (AddGene: 29656). Plasmids were freshly transformed into the E ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant strains harbouring pTEC19 plasmid (Addgene, #30178) and producing the fluorescent protein E2-Crimson were grown in Middlebrook 7H9 broth supplemented with 0.2% glycerol (v/v ...
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bioRxiv - Cell Biology 2021Quote: Nfic-HA tagged cDNA was purchased from Addgene (https://www.addgene.org/31403/) and subcloned into the lentiviral vector pLVX-puro using XhoI and XbaI ...
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bioRxiv - Microbiology 2022Quote: ... C-terminal Flag-tagged pCAGGS-SARS2-S-D614 (Addgene plasmid #156420) and pCAGGS-SARS2-S-G614 (Addgene plasmid #156421 ...
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bioRxiv - Cancer Biology 2020Quote: Flag-tagged PAPR1 WT (PARP1-Flag; #111575) was purchased from Addgene. The Flag-tagged PARP1 R138C mutant was generated by the site-directed mutagenesis Kit (Agilent ...
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bioRxiv - Cell Biology 2021Quote: ... Envy-tagged strains were engineered by capturing GFP-Envy from Addgene plasmid #60782 using PEP4 or VPH1-specific primers as described (Slubowski et al. ...
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bioRxiv - Molecular Biology 2023Quote: N-terminal histidine tagged wild-type VCP was obtained from Addgene (plasmid #12373 ...
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bioRxiv - Cell Biology 2023Quote: ... Anti-mCherry GST-tagged nanobodies (Addgene #70696, (Katoh et al, 2016)) were expressed and purified according to published protocol (Katoh et al. ...
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bioRxiv - Developmental Biology 2024Quote: ... The gene sequences encoding N-terminally mCherry tagged Drp1 (Addgene, 49152) were inserted between the EcoRI and PstI sites of the pCDNA vector (mCherry-Drp1) ...
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bioRxiv - Cell Biology 2024Quote: ... Plasmids used were coding for HA-tagged KDM4A/JMJD2A (Addgene #24180) or for an irrelevant HA-tagged construct HA-Tsg5’ as negative control (24).
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bioRxiv - Neuroscience 2024Quote: ... USA) and CXCR7-Tango plasmid (Flag-tagged ACKR3; Plasmid #66265, Addgene) were used for mammalian expression of the eGFP and Flag-ACKR3 protein ...
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bioRxiv - Genetics 2019Quote: Plasmid pF2-bio-His was obtained from Addgene (plasmid #52179) and human F2 cDNA was amplified using primers containing vector sequence homology (Table S1) ...
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bioRxiv - Biochemistry 2021Quote: ... SARS-CoV-2 3xFlag-His-nsp5CS-nsp14 (Addgene ID 169163) and SARS-CoV-2 nsp14/nsp10-His-3xFlag (Addgene ID 169164 ...
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bioRxiv - Biochemistry 2021Quote: Plasmids SARS-CoV-2 nsp10-His-3xFlag (Addgene ID 169162), SARS-CoV-2 3xFlag-His-nsp5CS-nsp14 (Addgene ID 169163 ...
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bioRxiv - Biochemistry 2020Quote: ... and cloned into a His-SUMO backbone (Addgene Plasmid #37507) for expression ...
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bioRxiv - Biochemistry 2022Quote: ... The acp ORF was amplified from pET29a-acp-His (Addgene) using primers listed in Table S5 and inserted into SacI- and BamH1-digested pET28b-Smt3-His10 to give pET28b-Smt3-His10-acp.
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bioRxiv - Molecular Biology 2020Quote: ... pET-His-hRan-Q69L was acquired from AddGene (catalog #42048) and pGEX-TEV-hCRM1 (XPO1 ...
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bioRxiv - Bioengineering 2023Quote: ... were transformed with the plasmid pET28b-RfxCas13d-His (Addgene 141322), which contain the recombinant CasRx gene ...
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bioRxiv - Genomics 2023Quote: ... were transformed with pET-28b-Cas9-His (Addgene, no. 47327) by heat shock following the manufacturers’ instructions ...
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bioRxiv - Genetics 2023Quote: His-SIRT5 expression plasmid was obtained from Addgene (plasmid #25487). Site-directed mutagenesis of WT SIRT5 was performed using the QuickChange Lightning site-directed mutagenesis kit (#210518 ...
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bioRxiv - Cell Biology 2022Quote: ... The plasmid encoding TDP-43-MBP-His6 for bacterial protein expression was a gift from Nicolas Fawzi (Addgene plasmid #104480 ...
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bioRxiv - Cell Biology 2024Quote: ... Mis18BP11-490 was cloned from a codon optimised sequence (GeneArt) into the pET His6 MBP TEV (14C, Addgene plasmid #48309 ...
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bioRxiv - Neuroscience 2024Quote: ... The oligodendrocyte-specific mouse-derived MBP promoter was amplified from the pAAV-MBP-2xNLS-tdTomato gift from Viviana Gradinaru (Addgene plasmid # 104054; http://n2t.net/addgene:104054; RRID:Addgene_104054) by KOD One PCR Master Mix using following primers 5’-ATGCTCTAGACTCGAGTCCTTCCTGCTTAGGCCGTG-3’ and 5’-CATGGTGGCGACCGGTCCTCCGGAAGCTGCTGTGGG-3’ to create the pAAV-mMBP-EGFP-WPRE-SV40pA25 ...
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bioRxiv - Cell Biology 2020Quote: ... eIF4B and Ded1 were tagged using the pML104 vector backbone (Addgene: 67638) while eIF4A was tagged using pML107 (Addgene ...