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Citations for Addgene :
1001 - 1035 of 1035 citations for hsa mir 940 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... To produce recombinant CK2 kinase the CKA2 gene was PCR amplified from yeast cDNA library and cloned into a pOPINF vector (a kind gift from Ray Owens, Addgene plasmid 26042) to encode a 3C-cleavable His6 fusion protein ...
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bioRxiv - Cell Biology 2022Quote: ... To produce EGFP-APEX2-ARHGAP29 the ARHGAP29 cDNA was sub-cloned into the C1-A2E vector (65) by PCR using HA-ARHGAP29 (Addgene plasmid #104154) as a template ...
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bioRxiv - Cell Biology 2023Quote: ... In a second step, this resulting plasmid and a PCR fragment amplified from pmTurquoise2-NES (Goedhart et al., 2012) (Addgene Plasmid# 36206) with primers 5’-TCAGTTGCTAGCCTCAAGCTTCGAATTCTG-3’ and 5’-AGAGTCAGCTCGAGATATCTTGTACGAGTCCAG-3’ ...
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bioRxiv - Biophysics 2023Quote: ... The resulting PCR products were then digested with XhoI and KpnI and cloned into a BacMam expression vector (pEZT-BM, Addgene plasmid # 74099)45 ...
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bioRxiv - Cell Biology 2023Quote: ... The KT binding domain sequence of 53BP1 (aa 1235-1616) was PCR-amplified from pcDNA5-FRT/TO-eGFP-53BP1 (Addgene plasmid #60813) and cloned into pB66 downstream to the Gal4 DNA-binding domain ...
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bioRxiv - Cell Biology 2023Quote: ... GFP-Sec61β in pBabe puro (a generous gift from Indra Chandrasekar) was created by PCR amplifying Sec61β (a gift from Gia Voeltz, Addgene plasmid #49154) and recombining into XhoI-SalI cut GFP pBabe puro ...
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bioRxiv - Immunology 2023Quote: ... The homology directed repair (HDR) template was generated by PCR from a template plasmid (a gift from Alexander Marson, Addgene plasmid #112021)(45 ...
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bioRxiv - Cancer Biology 2023Quote: ... 5’ and 3’ fragments containing incorporated mutated gene ORF sequences were amplified by PCR from the pDONR223-TP53 WT plasmid (Addgene; Plasmid #82754). In this case ...
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bioRxiv - Biochemistry 2024Quote: We also constructed pFGH1-UTG-mTagBFP2 as empty vectors by inserting PCR-amplified mTagBFP2 from pLKO.1 - TRC (a gift from Timothy Ryan; Addgene plasmid #191566) into digested backbone from pFGH1-UTG ...
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bioRxiv - Cell Biology 2024Quote: ... a fragment containing mTurquoise2 and the mSTING CDN was amplified by PCR and cloned in a pLex306 backbone (David Root, Addgene plasmid #41391). The sequence of E ...
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bioRxiv - Neuroscience 2019Quote: ... For pIRESneo3-Str-KDEL_SBP-EGFP-Nrxn1α a PCR fragment was inserted into pIRESneo3-Str-KDEL_SBP-EGFP-GPI (kindly provided by Franck Perez, Institute Curie, France; Addgene plasmid #65294; RRID: Addgene_65294) digested with FseI and PacI ...
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bioRxiv - Cell Biology 2022Quote: ... Cloning of KNL1RVSF/AAAA-dCas9 was achieved by inserting KNL1 PCR product (aa1-86, amplified from Addgene plasmid #45225, (Liu et al., 2010)) into XhoI-digested pENTR-dCas9 (no ATG ...
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bioRxiv - Molecular Biology 2021Quote: ... cDNAs were used as PCR templates for cloning into modified pFastBac vectors (derivatives of 438-A and 438-C; Addgene 55218 and 55220) by ligation independent cloning (LIC)37 ...
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bioRxiv - Cell Biology 2019Quote: ... pCytoplasm GFP was constructed by inserting PCR fragments of histone H3 promoter and the GFP ORF sequentially into the backbone vector pFGL1010 (Addgene #119081, sulfonylurea resistance); NOX1 and NOX2 deletion constructs were generated by inserting PCR amplified 5’ UTR and 3’ UTR fragments of the target genes sequentially into backbone vector pFGL821 (Addgene #58223 ...
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bioRxiv - Cell Biology 2019Quote: ... NOX1 and NOX2 deletion constructs were generated by inserting PCR amplified 5’ UTR and 3’ UTR fragments of the target genes sequentially into backbone vector pFGL821 (Addgene #58223, hygromycin resistance), flanking the HPH (hygromycin B phosphotransferase ...
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bioRxiv - Genetics 2020Quote: ... from pBPZpGAL4DBDUw or a p65AD-Zip-containing PCR fragment amplified with oligo pair 3857/3858 from pBPp65ADZpUw (both plasmids (Addgene 26233 and 26234) were gifts from Gerald Rubin (Pfeiffer et al. ...
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bioRxiv - Microbiology 2020Quote: ... aureus dCas9 was PCR amplified from pX603-AAV-CMV::NLS-dSaCas9(D10A,N580A)-NLS-3xHA-bGHpA (a gift from Dr. F. Zhang; Addgene plasmid # 61594 (39)) and inserted into a derivative of pBOMB4-Tet::L2 (kind gift of Dr ...
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bioRxiv - Molecular Biology 2022Quote: ... Two donor plasmids were generated by overlap PCR ∼500bp Ythdc1 genomic sequence flanking BSD/HygR-P2A-mAID-mCherry2 sequence (Addgene plasmid #121180, #121183) and cloning into pMD20-T vectors by T-A ligation ...
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bioRxiv - Genetics 2024Quote: ... We next used the Gateway™ LR Clonase™ II Enzyme mix to recombine each of the four PCR products into the pBID-UASC-G backbone (Addgene Plasmid #35202), which contains a φC31 integrase compatible attB sequence and UAS binding sites for the GAL4 expression system (Wang et al ...
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bioRxiv - Synthetic Biology 2023Quote: ... was cloned by generating PCR fragments from the pTetQCas-8+IS186 and the pTnsABC construct (gift from Sheng Yang (Addgene plasmid # 170636; & #1306331)) using the GeneArt™ Gibson Assembly HiFi Master Mix (InvitrogenTM) ...
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bioRxiv - Microbiology 2021Quote: ... the open reading frame was amplified by PCR from the pDONR223 SARS-CoV-2 NSP5 plasmid (Addgene, #141259, a gift from Fritz Roth [32]), including an ATG start codon in the forward primer and a TAA stop codon in the reverse primer (MPro_Fw ...
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bioRxiv - Biophysics 2020Quote: ... The pCNDA3.1-jRGECO1a plasmid was cloned by assembling PCR-amplified fragments from pGP-CMV-NES-jRGECO1a (Addgene # 61563, a gift from Dr. Douglas Kim57) and pCDNA3.1 ...
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bioRxiv - Microbiology 2020Quote: ... Plasmid pVpr.IN.mScarlet encoding a Vpr.IN.mScarlet fusion protein with a HIV-1 protease recognition site between Vpr and IN was constructed from pVpr.IN.eGFP (Albanese et al., 2008) by PCR amplification of the mScarlet gene from pmScarlet C1 (Addgene Cat#85042; (Bindels et al., 2017)) (primers used for PCR ...
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bioRxiv - Genetics 2022Quote: Human PUM1 full-length cDNA was amplified by PCR and subcloned in a pRK5 plasmid containing the Myc tag sequence (Addgene, pRK5-Myc-Parkin #17612) at the N-terminal by using SalI (New England Biolabs ...
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bioRxiv - Microbiology 2023Quote: ... The coding sequence for the Tn903.1 was amplified by PCR from the pET28 (T7pCONS/TIR2)-sfGFP plasmid (Addgene plasmid #154464;(Shilling, et al. 2020)) using the primers P7 and P8 ...
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bioRxiv - Microbiology 2023Quote: ... The coding sequence for the α-ALFA NB was amplified by PCR from the mEGFP-NbALFA plasmid (Addgene plasmid #159986; (Jin, et al. 2021)) using the primers P3 and P4 ...
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bioRxiv - Genetics 2024Quote: ... The denAsCas12a open reading frame was PCR amplified from pCAG-denAsCas12a(E174R/S542R/K548R/D908A)-NLS(nuc)-3xHA-VPR (RTW776) (Addgene plasmid # 107943, from 31). AsCas12a variants described were generated by using the denAsCas12a open reading frame as starting template and introducing the specific mutations encoded in overhangs on PCR primers that serve as junctions of Gibson assembly reactions ...
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bioRxiv - Molecular Biology 2024Quote: ... a 24-well dish was transfected with 250ng of px33050 containing the RBBP6-targeting guide and 250ng of PCR product containing the dTAG degron preceded by a blasticidin or puromycin selection marker (derived from Addgene plasmid 91792 and 9179324). Jetprime (Polyplus ...
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bioRxiv - Bioengineering 2022Quote: ... the modules in these plasmids (split Cas9, MCP, sgRNA cassette) were PCR-amplified and recloned into 2 lentiviral plasmids (pLKO.1 neo, Addgene #13425; pLJM-EGFP, Addgene #19319) and ...
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bioRxiv - Microbiology 2020Quote: ... pTRIP-SFFV-tagBFP-2A-hACE2 and pTRIP-SFFV-TagRFP657-2A-TMPRSS2 constructs were obtained by PCR from pLenti6-hACE2-BSD (hACE2 sequence from Addgene #1786 subcloned into pLenti6-BSD) and pCSDest-TMPRSS2 (Addgene #53887 ...
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bioRxiv - Microbiology 2020Quote: ... A miniTn7 element containing dcas9 was constructed by PCR-amplifying the tetR-tetP-dcas9-rrnBT1-T7Te fragment from pdCas9-bacteria (Addgene #44249, gift of Stanley Qi) with primers containing SpeI and PstI sites ...
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bioRxiv - Cell Biology 2023Quote: The sequences encoding STIM1 and RspA-NFAST were amplified by PCR from respectively the human STIM1-YFP plasmid (Addgene #19754, a gift from A. Rao) and the pAG573 FRB-RspA(N)-IRES-mTurquoise214 plasmid ...
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bioRxiv - Cell Biology 2024Quote: To generate RRBP1 KO HEK293T cells expressing EGFP-FLAG-APEX2-ePTS1 single gRNA transcriptional cassette prepared by PCR and pCAS9-mCherry empty (Addgene, 80975 (Schmid- Burgk et al., 2016)) were co-transfected into HEK293T cells expressing EGFP-FLAG-APEX2- ePTS1 ...
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bioRxiv - Microbiology 2024Quote: ... Roughly 1,000 base pairs upstream and downstream of the desired deletion location were amplified via PCR and assembled into allelic exchange vector pIMAY (Addgene plasmid # 68939, gifted by Tim Foster) (69 ...
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bioRxiv - Microbiology 2021Quote: ... The coding sequence from amino acid 747E to 1061K was amplified by PCR from the pDONR207 SARS-CoV-2 NSP3 plasmid (Addgene, #141257, a gift from Fritz Roth[32]), including a Kozak sequence and ATG start codon in the forward primer and a TAA stop codon in the reverse primer (PLPcd_Fw ...