Dematin Cell Based ELISA Kit

This Dematin cell based ELISA kit allows for the detection of Dematin and the effects that certain stimulation conditions have on Dematin expression in different cell lines. Qualitative determination of Dematin concentration is achieved by an indirect ELISA format. In essence, the Dematin is captured by anti-Dematin antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.

• Supplier: CD BioSciences
• Product #: CK10095