Plant Tryptophan (Trp) ELISA Kit

The ELISA kit applies the double-antibody sandwich method to determine the level of Plant Tryptophan (Trp)in plant samples. Purified Plant Tryptophan (Trp)antibodies are coated onto a microplate to form a solid-phase antibody. To these coated monoclonal antibody wells, Plant Tryptophan (Trp)is added, followed by an HRP-conjugated Plant Tryptophan (Trp)antibody, forming an antibody-antigen-enzyme-labeled antibody complex. After thorough washing, the substrate TMB is added and turns blue under the catalysis of HRP, and then changes to a final yellow color due to the addition of acid. The color intensity is directly proportional to the amount of Plant Tryptophan (Trp)present in the sample. The absorbance (OD value) is measured at 450 nm using an ELISA reader, and the concentration of Plant Tryptophan (Trp)in the plant samples is calculated based on a standard curve.

• Supplier: Lifeasible
• Product #: PEK-166
• specs: 96T/48T