Nuclease, Micrococcal
Chromatographically purified to be essentially homogeneous chromatographically and electrophoretically (SDS-PAGE). A lyophilized powder.
| Supplier | Worthington Biochemical |
|---|---|
| Product # | LS004798 |
| CAS # | 9013.53.0 |
| Pricing | 45 ku, $211.00 |
| Product Code | NFCP |
| Activity/Specification | ≥6,000 units per mg protein |
| UNSPSC Code(s)* | 12352204.0 |
| Source | Staphylococcus aureus (Strain Foggi) |
| Storage Conditions | Store at 2-8°C. |
Citations for this product:
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bioRxiv - Genomics 2022Quote: ... 100 ul NP buffer supplemented with 40 units of Micrococcal Nuclease (Worthington MNase, LS004798) were added and samples were incubated for 20 minutes at 37°C ...
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bioRxiv - Genomics 2020Quote: ... then digested with a concentration gradient of MNase (Worthington Biochemical Corporation #LS004798)for 30 min at 37°C ...
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bioRxiv - Molecular Biology 2021Quote: ... High digests (80% mononucleosomes) required 50U of micrococcal nuclease (Worthington, LS004798) and for the low digests ...
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bioRxiv - Developmental Biology 2019Quote: ... Cells then were resuspended in 100 μl 1X PBS with CaCl2 (final concentration: 1mM) and TritonX100 (final concentration: 0.1%) and incubated with 5 Units micrococcal nuclease (Worthington Biochemical, LS004798) at 37°C for 3 min ...
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bioRxiv - Systems Biology 2019Quote: ... 50μl of this suspension were digested with micrococcal nuclease (LS004798, Worthington Biochemical Corporation ...
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bioRxiv - Genomics 2020Quote: ... Chromatin was fragmented into ∼90% mononucleosome and ∼10% dinucleosome with Micrococcal Nuclease (MNase) (Worthington Biochem #LS004798). We recommend titrating the MNase digesting condition for each sample ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 mM β-mercaptoethanol and 0.075% NP-40) before being exposed to MNase (Worthington, LS004798) digestion titration to generate the appropriate size of chromatin fragments as follow ...
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bioRxiv - Genomics 2020Quote: ... Chromatin digestion was carried out at 30°C for 12 minutes with different concentrations of micrococcal nuclease (MNase, Worthington Biochemicals; cat# LS004798). The digestion was stopped by adding EDTA to final concentration of 10 mM ...
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bioRxiv - Genetics 2020Quote: ... followed by addition of 4 U MNase (Worthington Biochemical LS004798; Lakewood, NJ) per 1 × 107 cells ...
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bioRxiv - Genomics 2022Quote: ... and pellet was re-suspended in 10ul of lysis buffer supplemented with 17.5 units of micrococcal nuclease (Worthington MNase, LS004798) and chilled on ice for 10 minutes ...