DNase Vial (D2)
A component of the Papain Dissociation System. This material is 0.22 micron membrane filtered and lyophilized in autoclaved vials. A vial reconstituted with 0.5 ml of EBSS or equivalent yields a solution of 2000 units/ml of deoxyribonuclease (1 mg/ml).
| Supplier | Worthington Biochemical |
|---|---|
| Product # | LK003170 |
| CAS # | 9003.98.9 |
| Pricing | 1 vi, $28.00 |
| Product Code | D2 |
| Activity/Specification | ≥1,000 units per vial |
| UNSPSC Code(s)* | 12352204.0 |
| Source | Bovine Pancreas |
| Storage Conditions | Store at 2-8°C. |
Citations for this product:
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bioRxiv - Genetics 2020Quote: ... supplemented with 10 μM Rock inhibitor and 33 μg/mL DNase I (Worthington, LK003170, only for days 30 and 52). The cells were dissociated using a P1000 and collected in a 15 mL tube capped with a 40 μm cell strainer ...
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bioRxiv - Neuroscience 2021Quote: ... permeabilized cells were incubated with DNAse I recombinase (3-3000 U/ml in 50 mM Tris-HCL, pH 7.5, 1/ mg/ml BSA: Worthington, LK003170) prior to incubation with TUNEL mixture.
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bioRxiv - Developmental Biology 2022Quote: ... 1 mg/ml of DNase (Worthington, cat no. LK003170) in HBSS (Gibco ...
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bioRxiv - Neuroscience 2022Quote: ... 33 μg/ml DNase I (Worthington, LK003170), and 45 uM Actinomycin D to block dissociation-induced transcription ...
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bioRxiv - Neuroscience 2023Quote: ... DNAse I was added (Worthington. Cat. LK003170) to a final concentration of 1 mg/ml before mechanical dissociation with fire polished Pasteur pipettes coated previously with 1% BSA in PBS ...
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bioRxiv - Neuroscience 2023Quote: ... and 500 units DNase (Worthington, LK003170) for 20 minutes ...
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bioRxiv - Developmental Biology 2024Quote: ... DNase I (1 mg/ml, Worthington Biochemical, LK003170) in Earle’s balanced salt solution (EBSS ...