HCV Protease FRET Substrate (RET S1)

• Supplier: Creative Peptides
• Product #: X21173
• MolecularWeight: 1548.6
• Sequence:
  • One Letter Code: Ac-DE-D(Edans)-EE-Abu-ψ-[COO]-AS-K(Dabcyl)-NH2
  • Three Letter Code: Ac-Asp-Glu-Asp(EDANS)-Glu-Glu-Abu-ψ-[COO]-Ala-Ser-Lys(DABCYL)-NH2
• Storage: -20°C
• Explanation: This peptide is a HCV protease substrate incorporating an ester bond between residues P1 and P1. Due to ready transesterification of the scissile bond to the acyl-enzyme intermediate, this substrate shows very high kcat/Km values, enabling detection of activity with subnanomolar nonstructural protein 3 (NS3 protease) concentrations. It is widely used for the continuous assay of NS3 protease activity. Substrate cleavage is proportional to the enzyme concentration with a detection limit for NS3 between 1 nM and 250 pM. Upon cleavage of this substrate, fluorescence can be monitored at Abs/Em = 355/500 nm.
• Reference:
  • 1. Lin, C. et al. J. Biol. Chem. 10, 1074 (2004);
  • 2. Kakiuchi, N. et al. J. Virol. Methods 80, 77 (1999);
  • 3. Liu, Y. et al. Anal. Biochem. 267, 331 (1999);
  • 4. Taliani, M. et al. Anal. Biochem. 240, 60 (1996).