Collagenase, Type 3
Lower in secondary proteolytic contaminant activities but with typical collagenase activity. Suggested for mammary primary cell isolation. A dialyzed, lyophilized powder.
| Supplier | Worthington Biochemical |
|---|---|
| Product # | LS004183 |
| CAS # | 9001.12.1 |
| Pricing | 5 gm, $900.00 |
| Product Code | CLS-3 |
| Activity/Specification | ≥100 units per mg dry weight |
| UNSPSC Code(s)* | 12352204.0 |
| Source | Clostridium histolyticum |
| Storage Conditions | Store at 2-8°C. |
Citations for this product:
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bioRxiv - Cell Biology 2021Quote: ... and 2 mg/mL collagenase type III (Worthington Biochemical Corporation, LS004183) and were left for digestion in a 37°C incubator with a 5% CO2 atmosphere for 2 hours ...
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bioRxiv - Cell Biology 2022Quote: ... ears of wild-type C57BL/6J mice were collected and mechanically disrupted before enzymatic dissociation in 10 mg/ml Collagenase IV (Worthington, LS004183), 0.2% FBS and 0.2 mg/ml DNase I (Worthington ...
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bioRxiv - Cancer Biology 2020Quote: ... 150 U/mL Collagenase type 3 (Worthington, LS004183), 20 µg/mL Liberase Blendzyme 2 (Roche ...
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bioRxiv - Cell Biology 2022Quote: ... ovarian cortex samples were digested in DMEM containing 25 mM HEPES and 2 mg/mL collagenase type III (Worthington Biochemical Corporation, LS004183) for 2 hours at 37°C with occasional swirling ...
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bioRxiv - Cancer Biology 2021Quote: ... dissected tumors were minced into fragments under sterile conditions and subjected to enzymatic digestion with Collagenase-Type3 (Worthington, Cat#: LS004183) at 2mg/ml ...
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bioRxiv - Cancer Biology 2022Quote: ... and 200 units/ml Collagenase-III (Worthington, LS004183). Tissue fragments were then incubated at 37°C for two hours ...
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bioRxiv - Cancer Biology 2022Quote: ... and 200 U/mL Collagenase-III (Worthington, LS004183) in DPBS at 37°C for 15 minutes ...
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bioRxiv - Cancer Biology 2022Quote: ... and 1,000 U of collagenase type III (Worthington Biochemical, cat# LS004183) in DMEM ...
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bioRxiv - Cancer Biology 2023Quote: ... The minced tumors were digested with 5ml to 10ml of 1 mg/ml Collagenase 3 (Worthington cat. no. LS004183) plus 0.5mg/ml Hyaluronidase (Sigma-Aldrich H3884 ...
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bioRxiv - Cancer Biology 2023Quote: ... and 1,000 U of collagenase type III (Worthington Biochemical, cat# LS004183) in DMEM (Euroclone ...