TAMRA phosphoramidite, 5-isomer
This phosphoramidite is used for synthesis of oligonucleotides 5’-labeled with TAMRA.,TAMRA
(carboxytetramethylrhodamine) is a xanthene dye from the rhodamine family with emission in the orange
spectrum range (maximum at 563 nm). This fluorophore is traditionally used as a FRET-acceptor
(and a quencher) in a pair with fluorescein (FAM) due to significant overlapping of their
spectra. Thus, this phosphoramidite is convenient for the synthesis of dual-labeled probes TaqMan, which
contain 5’-terminal TAMRA and FAM in the middle of the sequence or at the 3’-end
(using Fluorescein dT Phosphoramidite and FAM
CPG, respectively).,TAMRA 5’-labeled oligonucleotides are commonly used for quantitative PCR
and fragment analysis (for example, for microsatellite marker analysis) because the equipment available has a detection
channel for TAMRA frequently.,The TAMRA dye is not stable in the presence of ammonium and
sterically non-hindered primary amines, so it is strongly recommended to follow specified
conditions for labeled oligonucleotide deprotection.,Coupling: 7.5 min.,Deprotection: tret-buthylamine : methanol : water 1 : 1 : 3 (v/v/v) («TAMRA cocktail») for 6 hours at 60 °C, then cool down to room temperature.,Due to complete and irreversible degradation of the TAMRA dye, do NOT use aqueous ammonium and AMA for deprotecting a modified oligonucleotide from the solid-phase support.
(carboxytetramethylrhodamine) is a xanthene dye from the rhodamine family with emission in the orange
spectrum range (maximum at 563 nm). This fluorophore is traditionally used as a FRET-acceptor
(and a quencher) in a pair with fluorescein (FAM) due to significant overlapping of their
spectra. Thus, this phosphoramidite is convenient for the synthesis of dual-labeled probes TaqMan, which
contain 5’-terminal TAMRA and FAM in the middle of the sequence or at the 3’-end
(using Fluorescein dT Phosphoramidite and FAM
CPG, respectively).,TAMRA 5’-labeled oligonucleotides are commonly used for quantitative PCR
and fragment analysis (for example, for microsatellite marker analysis) because the equipment available has a detection
channel for TAMRA frequently.,The TAMRA dye is not stable in the presence of ammonium and
sterically non-hindered primary amines, so it is strongly recommended to follow specified
conditions for labeled oligonucleotide deprotection.,Coupling: 7.5 min.,Deprotection: tret-buthylamine : methanol : water 1 : 1 : 3 (v/v/v) («TAMRA cocktail») for 6 hours at 60 °C, then cool down to room temperature.,Due to complete and irreversible degradation of the TAMRA dye, do NOT use aqueous ammonium and AMA for deprotecting a modified oligonucleotide from the solid-phase support.
| Supplier | BOC Sciences |
|---|---|
| Product # | A19-0095 |
| Pricing | Inquire |
| Molecular Weight | 727.83 |
| Molecular Formula | C40H50N5O6P |
| Canonical SMILES | CC(C)N(C(C)C)P(OCCC#N)OC1CCC(CC1)NC(=O)C2=CC3=C(C=C2)C4(C5=C(C=C(C=C5)N(C)C)OC6=C4C=CC(=C6)N(C)C)OC3=O |