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No products found
because this supplier's products are not listed.
Quote:
... 2 µl was used in real time RT-PCR reactions performed on CFX96 Touch Real-Time PCR Detection System (Bio-Rad) in a 20µl volume with iQ SYBR®Green Supermix (Bio-Rad) ...
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No products found
because this supplier's products are not listed.
Quote:
... and quantitative real-time RT-PCR (qRT-PCR) was performed using the ViiA 7 real-time PCR detection platform (Applied BioSystems). All gene specific primers used for qRT-PCR were evaluated by BLASTN search against the Fg and Bd genomes to exclude unspecific annealing and are listed in Supplemental Table 2 ...
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No products found
because this supplier's products are not listed.
wei zhao, et al.,
bioRxiv - Microbiology 2018
Quote:
... Quantitative real-time RT-PCR was performed using theSYBR PrimeScript RT-kit(TaKaRa Bio) according to the manufacturer’s instructions.The relative transcript levels were analyzed using the ΔΔCt method ...
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No products found
because this supplier's products are not listed.
Zhifeng Li, et al.,
bioRxiv - Microbiology 2018
Quote:
... Real-time RT-PCR was performed using the QuantiTech RT-PCR kit (Qiagen). The primers were designed as previously described and used in a one-step real-time RT-PCR [18] ...
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No products found
because this supplier's products are not listed.
Wei-Hsiang Lin, Richard A. Baines,
bioRxiv - Neuroscience 2018
Quote:
Quantitative RT-PCR was performed using a SYBR Green I real-time PCR method (Roche, LightCycler® 480 SYBR Green I Master ...
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No products found
because this supplier's products are not listed.
María Eugenia Loureiro, et al.,
bioRxiv - Microbiology 2017
Quote:
... and a Real-Time PCR Detection System (ABI). For BSL-4 infections ...
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No products found
because this supplier's products are not listed.
Charlotte Rich, et al.,
bioRxiv - Plant Biology 2018
Quote:
... in a Stratagene Mx3005P Real-time PCR Detection System (Agilent Technologies) following a standard protocol ...
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No products found
because this supplier's products are not listed.
Quote:
... a real-time PCR instrument (Eco Real-Time PCR System, Illumina) was used with the same primers (Table S1 ...
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No products found
because this supplier's products are not listed.
,
bioRxiv - Neuroscience 2018
Quote:
qRT-PCR experiments were performed using Realplex real time PCR detection system (Eppendorf, Germany) as previously described (Singh et al. ...
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No products found
because this supplier's products are not listed.
Xingjie-Bao, et al.,
bioRxiv - Genetics 2018
Quote:
... QRT-PCR was conducted with the GoTaq® Real-Time PCR Systems reagent kit (Promega, Beijing, China), and miRcute Plus miRNA Real-Time PCR Systems reagent kit (TianGen ...
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No products found
because this supplier's products are not listed.
Quote:
... Real-time quantitative PCR (RT-qPCR) was performed using the SensiFast SYBR No-ROX Kit (Bioline) on a Rotor-Gene Q machine (Qiagen) ...
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No products found
because this supplier's products are not listed.
Crisanta Serrano-Collazo, et al.,
bioRxiv - Immunology 2019
Quote:
... Real-time RT-PCR (TaqMan) assay-specific primers and probes for ZIKV were designed by Sigma-Aldrich (St Louis, MO) following the protocol developed by the Molecular Diagnostics and Research Laboratory Centers for Disease Control and Prevention (CDC) ...
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No products found
because this supplier's products are not listed.
Xingjie-Bao, et al.,
bioRxiv - Genetics 2018
Quote:
... and miRcute Plus miRNA Real-Time PCR Systems reagent kit (TianGen, Beijing, China) following the manufacturer’s instructions ...
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No products found
because this supplier's products are not listed.
Qinchuan Wang, et al.,
bioRxiv - Evolutionary Biology 2019
Quote:
... and quantified by qPCR on a Bio-Rad CFX Connect Real-Time PCR detection system with the NEBNext Library Quant Kit for Illumina (New England Biolabs, catalog # E7630L). The libraries were normalized to 10 nM ...
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No products found
because this supplier's products are not listed.
Quote:
... The real-time PCR was subsequently performed on a Bio-Rad CFX96 Touch™ real-time PCR detection system using SYBR green based PrecisionFAST with LOW ROX qPCR kit (Primerdesign). The specificity of PCR primers were verified by both melt curves and agarose gel electrophoresis ...
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No products found
because this supplier's products are not listed.
Quote:
... slides were washed 3 times for 15 minutes in 0.2X SSC and mounted with VECTASHIELD with DAPI (Vector Labs). For the in situ experiment described in Figure 4j-m ...
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No products found
because this supplier's products are not listed.
Quote:
... with 100nM miR scrambled or miR-181a mimic (100nM; GE Healthcare) (Soriano-Arroquia et al. ...
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No products found
because this supplier's products are not listed.
Quote:
... blots were hybridized overnight at 42°C with 5’-end P32-labelled oligonucleotides in Roti-Hybrid-Quick buffer (Roth) and washed three times at 42°C for 15min with SSC buffer (SSC 20X: 3M NaCl, 300mM sodium citrate, pH 7) containing 0.1% of SDS (5X ...
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No products found
because this supplier's products are not listed.
Quote:
... Cells were regularly cultured for three days in antibiotic-free medium and tested for mycoplasma using either the LookOut Mycoplasma PCR Detection Kit or the MycoAlert mycoplasma detection kit (Lonza).
-
No products found
because this supplier's products are not listed.
Quote:
... Trypsinised cells were subjected to quantitative real-time PCR and 25 mM GSH measured in the Accuri C6 flow cytometer (BD Biosciences). Data were analysed using GraphPad Prism 5.0 and FlowJo 7.6 (Tree Star).
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No products found
because this supplier's products are not listed.
Quote:
... or the Ingenio electroporation kit (Mirus, #MIR 50117) with 1.5 µg of either control siRNA (ON-TARGETplus Non-Targeting Pool ...
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No products found
because this supplier's products are not listed.
Quote:
... After purification of the RT-PCR products using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel) according to the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Arka Baksi, et al.,
bioRxiv - Genomics 2018
Quote:
... In-situ Apoptosis Detection Kit (Abcam) was used to check for the apoptotic cells in the tissue sections according to the Manufacturer’s protocol ...
-
No products found
because this supplier's products are not listed.
Yapeng Su, et al.,
bioRxiv - Bioengineering 2019
Quote:
... MART1and PFK transcripts were analyzed by SYBR Green–based real-time quantitative RT-PCR (qRT-PCR) using specific primers purchased from Santa Cruz. Data were normalized to the expression of RPL19 and are expressed as fold changes.
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No products found
because this supplier's products are not listed.
Philip R Nicovich, et al.,
bioRxiv - Neuroscience 2019
Quote:
... RT incubation in 2X SSC containing 8 µg/µl Brilliant Violet 421™ Streptavidin (BioLegend, Cat. No. 405225) and 0.05% Tween 20 ...
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No products found
because this supplier's products are not listed.
Daria Esterházy, et al.,
bioRxiv - Immunology 2018
Quote:
... Colonization or depletion was verified by real time PCR of fecal DNA (Quick-DNA Fecal/Soil microbe miniprep kit, Zymo research, Cat. No. D6010). PCR was performed in presence of Power SYBR Green PCR master mix (Applied Biosystems ...
-
No products found
because this supplier's products are not listed.
Hugo Arasanz, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... Cell growth and survival were monitored in real time using iCELLigence and xCELLigence real-time cell analysis (RTCA ACEA Biosciences) by seeding 20.000 cells and 5.000 cells respectively ...
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No products found
because this supplier's products are not listed.
Christopher R. Merritt, et al.,
bioRxiv - Pathology 2019
Quote:
... Secondary detection was performed this the Bond Polymer Refine Detection Kit (Leica, DS9800). Tissue sections were imaged using a Nikon Eclipse Ci light microscope with 4x or 20x objectives and Nikon Elements software.
-
No products found
because this supplier's products are not listed.
Quote:
... TSA Plus DIG detection Kit (Perkin Elmer) was used for signal amplification ...
-
No products found
because this supplier's products are not listed.
Quote:
... using 2X HoTaq Real Time PCR kit (McLab) and the appropriate primer-probe combinations described in (Calvanese et al. ...
-
No products found
because this supplier's products are not listed.
Yu-Meng Wang, et al.,
bioRxiv - Developmental Biology 2018
Quote:
... The levels of mature miR-197 were determined with All-in-One™ miRNA qRT-PCR Detection Kit (GeneCopoeia, QP015). The levels of pri-miR-197 were detected with TaqMan pri-microRNA assay kit (Applied Biosystems ...
-
No products found
because this supplier's products are not listed.
Quote:
... The qPCR was performed using EvaGreen Real-time PCR (Biotium, Hayward, USA) according to the manufacturer’s recommendations using 1µl EvaGreen Real-time PCR (Biotium ...
-
No products found
because this supplier's products are not listed.
Quote:
... RT-PCR was performed using the Maxime PCR Premix Kit (Intron Biotechnology, Seoul, Korea). Transcription of HC and LC genes was confirmed with primers as described above ...
-
No products found
because this supplier's products are not listed.
Quote:
... Quantitative Real time PCR (qPCR) was performed using Takyon ROX SYBR 2X MasterMix (Eurogentec UF-RSMT-B0701) as a fluorescent detection dye ...
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No products found
because this supplier's products are not listed.
Quote:
... real-time (SMRT) cells at Pacific Biosciences (PacBio). Sequence data were generated using the PacBio RSII instrument with P6v2 polymerase binding and C4 chemistry kits (P6-C4 ...
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No products found
because this supplier's products are not listed.
Anna V. Protasio, et al.,
bioRxiv - Molecular Biology 2019
Quote:
... Real time quantitative PCR was performed with KAPA SYBR FAST qPCR Master Mix (Sigma-Aldrich - Merck, UK, cat no KK4602) according to manufacturer’s instructions in a StepOnePlus Real-Time PCR System (Thermo Fisher Scientific ...
-
No products found
because this supplier's products are not listed.
Astrid Hoermann, et al.,
bioRxiv - Synthetic Biology 2020
Quote:
... RT-PCR was performed with RedTaq Polymerase (VWR) with primers 51-Scorpine-F and 117-CP-ctrl-R on Sco-CP (1267bp) ...
-
No products found
because this supplier's products are not listed.
Katrín Halldórsdóttir, Einar Árnason,
bioRxiv - Evolutionary Biology 2015
Quote:
... We quantified the individual libraries with fluorescent detection using a Quant-iTTM PicoGreen® dsDNA Assay Kit Quantit kit on a Spectramax i3x Multi-Mode Detection Platform (Molecular Devices). We determined the size distribution of 12 randomly chosen libraries ...
-
No products found
because this supplier's products are not listed.
Xin-Chun Zhao, et al.,
bioRxiv - Cancer Biology 2018
Quote:
... Reagents used were: Chemiluminescent Western detection kit (Cell Signaling); cycloheximide (CHX ...
-
No products found
because this supplier's products are not listed.
Ruiqing Zhang, Xinhua Chen, Hao Wen,
bioRxiv - Pathology 2017
Quote:
Blood samples were collected at different time points for detection of IgG antibodies using a nephelometric technique (Beckman Array 360 ...
-
No products found
because this supplier's products are not listed.
José Miguel Urra, et al.,
bioRxiv - Immunology 2020
Quote:
... The infection by SARS-CoV-2 was confirmed in all patients included in the study by the real time reverse transcriptase-polymerase chain reaction (RT-PCR) assay from Abbott Laboratories (Abbott RealTime SARS-COV-2 assay ...
-
No products found
because this supplier's products are not listed.
Quote:
... The cDNA for RT-PCR was synthesized using an MMLV RT Kit (Evrogen, Russia) according to the manufacturer’s recommendations employing oligo(dT)17-primers from 2 µg total RNA after DNase treatment ...
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Sample-loading buffer and protein stain in one
Instant results - see protein bands immediately...
Cat# K-11053-B30,
3 ml, USD $189.00/ea
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M. Victoria Barja, et al.,
bioRxiv - Plant Biology 2020
Quote:
... WesternBright ECL Western blotting detection kit (Advansta) and Amersham ECL Prime Western Blotting Detection Kit (GE Healthcare ...
-
No products found
because this supplier's products are not listed.
Priya Chatterji, et al.,
bioRxiv - Physiology 2018
Quote:
CytoID Autophagy Detection Kit (Enzo Life Sciences) was used to stain single cell suspensions of crypt-enriched intestinal epithelium (1:100 in DPBS supplemented with 10% FBS at 37°C for 30 min ...
-
No products found
because this supplier's products are not listed.
Martina Radli, et al.,
bioRxiv - Biochemistry 2017
Quote:
... The membrane was washed two times with TBS-T and two times with TBS prior to detection using the Odyssey scanner (LI-COR).
-
No products found
because this supplier's products are not listed.
Yan Chen, et al.,
bioRxiv - Molecular Biology 2019
Quote:
... the miR-141 indicator is from Addgene (#67632). To generate 3’UTR luciferase reporters for BCL6 ...
-
No products found
because this supplier's products are not listed.
Seongjin Park, et al.,
bioRxiv - Biophysics 2020
Quote:
... the Z-drift was prevented in real time by a built-in focus lock system (Nikon Perfect Focus).
-
No products found
because this supplier's products are not listed.
Quote:
... Spermatogonial stem cells (SSC) were enriched using THY1+ microbeads (Miltenyi Biotec; 131-049-101) followed by their isolation on magnetic activated cell sorting (MACS ...
-
No products found
because this supplier's products are not listed.
Maurício Aurelio Gomes Heleno, et al.,
bioRxiv - Pharmacology and Toxicology 2020
Quote:
... Annexin V/propidium iodide apoptosis detection kits were obtained from R&D Systems Inc ...
-
No products found
because this supplier's products are not listed.
Quote:
... RT products were circularized with CircLigase (Epicentre) for 60 minutes at 60˚C ...