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No products found
because this supplier's products are not listed.
Ilja Vietor, et al.,
bioRxiv - Cell Biology 2019
Quote:
... Quantitative RT-PCR was performed using Taqman probes and primer sets (Applied Biosystems) specific for CD36 (assay ID Mm00432398_m1) ...
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No products found
because this supplier's products are not listed.
Quote:
... and mir-34a (Qiagen MiScript primer assay). Real time PCR data was analyzed using the 2−▵▵Ct method24,25 and expressed as fold enrichment.
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No products found
because this supplier's products are not listed.
John A. Bennett, et al.,
bioRxiv - Pharmacology and Toxicology 2018
Quote:
... Prime PCR primer sets were purchased from Bio-Rad. Expression results were calculated by ΔΔCT method and were normalized to the reference genes GAPDH and βActin.
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No products found
because this supplier's products are not listed.
Prashanth Kumar Guthikonda, et al.,
bioRxiv - Developmental Biology 2017
Quote:
... Quantitative RT-PCR was set up with SYBR Premix Ex Taq II (Takara Bio) using specific RP gene primers (supplementary Table 1 ...
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No products found
because this supplier's products are not listed.
Quote:
... and indexed with ScriptSeq™ Index PCR primers set 1 (Epicentre, Illumina) following the standard protocol ...
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No products found
because this supplier's products are not listed.
Quote:
... Primers with published sequences (17) were purchased from Sigma-Aldrich. Expression of genes of interest was normalized against expression of GAPDH ...
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No products found
because this supplier's products are not listed.
Quote:
... RT-PCR performed using specific primers sets (Supplementary Tables 2, 3) and GoTaq® Green Master Mix (Promega, Madison, WI). (30-40 cycles ...
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No products found
because this supplier's products are not listed.
Vaseef A. Rizvi, Maharnob Sarkar, Rahul Roy,
bioRxiv - Microbiology 2020
Quote:
... The rRNA-subtracted circular product is subjected to a final round of PCR amplification with Illumina adaptor primers [17] using Phusion polymerase (NEB). All the libraries were then quantified ...
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No products found
because this supplier's products are not listed.
Sway P. Chen, Harris H. Wang,
bioRxiv - Synthetic Biology 2019
Quote:
... We performed colony PCR using the primer set p898/p415 with KAPA2G Robust HotStart ReadyMix (Kapa Biosystems) to amplify transposon-pTarget junctions ...
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No products found
because this supplier's products are not listed.
Quote:
... A total of 60 libraries were created by PCR amplification with Illumina barcoding primers at 17 reaction cycles and quantified using a Bioanalyzer DNA 1000 kit (Agilent). Libraries were then pooled and sequenced on a HiSeq 2000 Sequencing system (Illumina).
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No products found
because this supplier's products are not listed.
Bonita H. Powell, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... TaqMan miRNA stem-loop RT primers/qPCR primer probe sets (ABI 4427975) (cel-miR-39 ID# 000200 Lot#P180110-003B10 ...
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No products found
because this supplier's products are not listed.
CF Hung, et al.,
bioRxiv - Cell Biology 2019
Quote:
... The cDNA was then diluted 1:5 and qRT-PCR reactions were set up in triplicate using primers synthesized from IDT and SensiMix SYBR & Fluorescin kit (Bioline, Meridian Bioscience). qRT-PCR was done using a Corbett Research RG 6000 thermocycler ...
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No products found
because this supplier's products are not listed.
Quote:
... and indexed with ScriptSeq™ Index PCR primers set 1 (Epicentre, Illumina) following the standard protocol ...
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No products found
because this supplier's products are not listed.
Alexander Yermanos, et al.,
bioRxiv - Immunology 2020
Quote:
... Whole blood was collected 31 and 32 days following a positive RT-PCR test and subjected to density gradient centrifugation using the Ficoll Paque Plus reagent (GE Healthcare, #17-1440-02). After separation ...
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No products found
because this supplier's products are not listed.
Quote:
... by overnight capillary transfer at RT using 20X SSC transfer buffer (3M NaCl, 0.3M Sodium citrate, pH 7). DNA was fixed on the membrane by UV-crosslinking at 1.2J/cm2 for 30s in HL-2000 Hybrilinker (UVP ...
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No products found
because this supplier's products are not listed.
Yan Chen, et al.,
bioRxiv - Molecular Biology 2019
Quote:
... the miR-141 indicator is from Addgene (#67632). To generate 3’UTR luciferase reporters for BCL6 ...
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No products found
because this supplier's products are not listed.
Philip R Nicovich, et al.,
bioRxiv - Neuroscience 2019
Quote:
... RT incubation in 2X SSC containing 8 µg/µl Brilliant Violet 421™ Streptavidin (BioLegend, Cat. No. 405225) and 0.05% Tween 20 ...
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No products found
because this supplier's products are not listed.
Quote:
... and 17 clones were sent for Sanger sequencing with the M13_F primer (Beckman Coulter Genomics).
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No products found
because this supplier's products are not listed.
Sharon Camacho, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... ITS (Lonza, 17-838Z), non-essenOal amino acids (Sigma ...
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No products found
because this supplier's products are not listed.
Jac M.M.J.G. Aarts, et al.,
bioRxiv - Evolutionary Biology 2018
Quote:
... HeLa cells were transfected using the TransIT-HeLaMONSTER Transfection Kit (MIR 2904) and HepG2 cells with TransIT-LT1 Transfection Reagent (MIR 2304) applying the standard protocols (both from Mirus Bio LLC ...
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No products found
because this supplier's products are not listed.
Yapeng Su, et al.,
bioRxiv - Bioengineering 2019
Quote:
... MART1and PFK transcripts were analyzed by SYBR Green–based real-time quantitative RT-PCR (qRT-PCR) using specific primers purchased from Santa Cruz. Data were normalized to the expression of RPL19 and are expressed as fold changes.
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No products found
because this supplier's products are not listed.
Darshana Kadekar, et al.,
bioRxiv - Immunology 2019
Quote:
... IL-17-BV786 (TC11-18H10, BD biosciences), IL-22-PE (1H8PWSR ...
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No products found
because this supplier's products are not listed.
Zhihua Liu, et al.,
bioRxiv - Microbiology 2020
Quote:
... Reverse transcription with ZIKA virus specific primer (Rev-AAGTGATCCATGTGATCAGTTGATCC) was performed using FastQuant RT Kit (Tiangen). Real-time PCR was done on 7900HT (ABI ...
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No products found
because this supplier's products are not listed.
Quote:
... cDNA-specific PCR primers were designed using Primer-BLAST (see Table 1) and purchased from Eurogentec (Seraing, Belgium). Gapdh was used as reference gene ...
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No products found
because this supplier's products are not listed.
Quote:
... cDNA was amplified by reverse transcriptase PCR (RT-PCR) using a Master Cycler Pro (Eppendorf, Hamburg, Germany) and visualized on a 1.5% agarose gel ...
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No products found
because this supplier's products are not listed.
Quote:
... 17 mg/mL Cellulysin (Calbiochem, La Jolla, CA), 17 mg/mL Cellulase RS (Yakult ...
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No products found
because this supplier's products are not listed.
Quote:
... the cells were incubated with DAPI diluted in PBS for 10 min at RT and washed before being mounted onto slides with Vectashield Hard Set (Vector Laboratories). Single-planed images were taken on a Nikon C2 confocal microscope using a 40X oil-immersion lens ...
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No products found
because this supplier's products are not listed.
David E. Ochayon, et al.,
bioRxiv - Immunology 2019
Quote:
... The inhibition of ADAM-17 (a disintegrin and metalloprotease-17) was performed by adding 8 μM TAPI-1 (Merck Millipore, Burlington, MA). TAPI-1 and MK2 IV were added 60 minutes before cytokine stimulation ...
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No products found
because this supplier's products are not listed.
Quote:
... Fluorescence filter sets from Chroma Technology and Semrock were used ...
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No products found
because this supplier's products are not listed.
Astrid Hoermann, et al.,
bioRxiv - Synthetic Biology 2020
Quote:
... RT-PCR was performed with RedTaq Polymerase (VWR) with primers 51-Scorpine-F and 117-CP-ctrl-R on Sco-CP (1267bp) ...
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No products found
because this supplier's products are not listed.
Pastor Jullian Fabres, et al.,
bioRxiv - Plant Biology 2020
Quote:
... RT-PCR products were purified using PCR Clean-up (Macherey-Nagel, 740609.250) following the manufacturer’s instructions ...
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No products found
because this supplier's products are not listed.
Quote:
... IL-17 (R&D Systems) and TNF-α (Thermo Fisher ...
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No products found
because this supplier's products are not listed.
Jessica M Snyder, et al.,
bioRxiv - Pathology 2020
Quote:
The serum IL-17 concentrations were quantified using a mouse IL-17 ELISA kit from Abcam (ab100702) according to the manufacturer’s instructions ...
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No products found
because this supplier's products are not listed.
Eleni Mimitou, et al.,
bioRxiv - Genomics 2018
Quote:
... separate PCR reactions were set up to generate the ADT library (SI-PCR and RPI-x primers), the hashtag library (SI-PCR and D7xx_s ...
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No products found
because this supplier's products are not listed.
Quote:
... triheptadecanoylglycerol (TG(17:0/17:0/17:0)) was purchased from Larodan AB (Solna ...
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No products found
because this supplier's products are not listed.
Quote:
... and using DENV RT primer/probe Mix kit (Genesig, Primerdesign Ltd., UK) according to the manufacturer’s protocol ...
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No products found
because this supplier's products are not listed.
Nicholas O’Toole, et al.,
bioRxiv - Neuroscience 2019
Quote:
Methylated and unmethylated DNA sets (Cat# D5017, pUC19 DNA set, Zymo research) were added as spike-in controls (2 ng spike-in control in 1 µg DNA ...
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No products found
because this supplier's products are not listed.
Quote:
Stellaris probe-sets for smFISH (Biosearch Technologies) were custom designed for intron and exon regions (see Appendix for oligo list ...
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No products found
because this supplier's products are not listed.
Quote:
... with the Ds-Red2 filter set (Leica). Induction of cDNA deletion was examined by genomic PCR using the primer sets 1 (MpEF-P_seqL1 and MpMPK1_c1131R_STP) ...
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No products found
because this supplier's products are not listed.
Quote:
... We calibrated the FSC and SSC gates to detect only yeast cells (FSC-PMT=681V, SSC-PMT=264V ...
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No products found
because this supplier's products are not listed.
Quote:
... The cDNA for RT-PCR was synthesized using an MMLV RT Kit (Evrogen, Russia) according to the manufacturer’s recommendations employing oligo(dT)17-primers from 2 µg total RNA after DNase treatment ...
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No products found
because this supplier's products are not listed.
Quote:
... miR-223−/− and miR-223-/y KO mice (B6.Cg-Ptprca Mir223tm1Fcam/J) were purchased from Jackson Laboratories and maintained on a CD45.1+ Background (B6.SJL-Ptprca Pepcb/BoyJ ...
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No products found
because this supplier's products are not listed.
Quote:
... then incubate in 2X SSC (Corning, cat#46-020-CM) for 5 min ...
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No products found
because this supplier's products are not listed.
Quote:
... miR-1002 negative scramble was ordered from Genscript for ligation into pAC-GAL4.
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No products found
because this supplier's products are not listed.
Quote:
Fluorescence correlation spectroscopy (FCS) data sets were acquired on a custom-built set-up with an inverted Nikon Eclipse TE2000 microscope (Nikon) equipped with a 100x oil immersion objective (Nikon ...
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No products found
because this supplier's products are not listed.
Tyler J. Marquart, et al.,
bioRxiv - Cell Biology 2019
Quote:
... and cloned into pCMV-miR (Origene). To generate replication-deficient adenovirus ...
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No products found
because this supplier's products are not listed.
Yasuhiko Chikami, et al.,
bioRxiv - Molecular Biology 2019
Quote:
... The RT-PCR were performed using AmpliTaq Gold DNA polymerase (Perkin Elmer, Boston, USA). PCR fragments were separated with electrophoresis using 1% agarose gel in tris-borate-EDTA (TBE ...
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No products found
because this supplier's products are not listed.
Quote:
Eight-week-old female CB-17 SCID mice (Charles River Laboratories ...
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No products found
because this supplier's products are not listed.
Syun-Wun Liang, et al.,
bioRxiv - Microbiology 2018
Quote:
... with a filter set (Olympus, U-MWU2 BP330~385).
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No products found
because this supplier's products are not listed.
Quote:
... For visualization of DAPI fluorescence filter set 49 (Carl Zeiss; excitation ...