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ssc mir 17 RT PCR Primer Set
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Cohesion Biosciences Sponsored
Stem-loop RT primer and PCR primer set to detect mature miRNA ssc-mir-17 expression.Cat# CPK3362, 1 nmol USD $100.0 Ask
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Thermo Fisher
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2019Quote: ... Quantitative RT-PCR was performed using Taqman probes and primer sets (Applied Biosystems) specific for CD36 (assay ID Mm00432398_m1) ... -
Qiagen
No products found because this supplier's products are not listed.bioRxiv - Molecular Biology 2018, published in Molecular Therapy - Nucleic Acids doi: 10.1016/j.omtn.2019.04.003Quote: ... and mir-34a (Qiagen MiScript primer assay). Real time PCR data was analyzed using the 2−▵▵Ct method24,25 and expressed as fold enrichment. -
Bio-Rad
No products found because this supplier's products are not listed.bioRxiv - Pharmacology and Toxicology 2018Quote: ... Prime PCR primer sets were purchased from Bio-Rad. Expression results were calculated by ΔΔCT method and were normalized to the reference genes GAPDH and βActin. -
Takara Bio
No products found because this supplier's products are not listed.bioRxiv - Developmental Biology 2017Quote: ... Quantitative RT-PCR was set up with SYBR Premix Ex Taq II (Takara Bio) using specific RP gene primers (supplementary Table 1 ... -
Illumina
No products found because this supplier's products are not listed.bioRxiv - Systems Biology 2019, published in npj Systems Biology and Applications doi: 10.1038/s41540-019-0121-4Quote: ... and indexed with ScriptSeq™ Index PCR primers set 1 (Epicentre, Illumina) following the standard protocol ... -
Millipore Sigma
No products found because this supplier's products are not listed.bioRxiv - Cancer Biology 2018, published in Clinical Cancer Research doi: 10.1158/1078-0432.ccr-18-1346Quote: ... Primers with published sequences (17) were purchased from Sigma-Aldrich. Expression of genes of interest was normalized against expression of GAPDH ... -
Promega
No products found because this supplier's products are not listed.bioRxiv - Cancer Biology 2017, published in Disease Models & Mechanisms doi: 10.1242/dmm.031716Quote: ... RT-PCR performed using specific primers sets (Supplementary Tables 2, 3) and GoTaq® Green Master Mix (Promega, Madison, WI). (30-40 cycles ... -
New England Biolabs
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2020Quote: ... The rRNA-subtracted circular product is subjected to a final round of PCR amplification with Illumina adaptor primers [17] using Phusion polymerase (NEB). All the libraries were then quantified ... -
Roche
No products found because this supplier's products are not listed.bioRxiv - Synthetic Biology 2019Quote: ... We performed colony PCR using the primer set p898/p415 with KAPA2G Robust HotStart ReadyMix (Kapa Biosystems) to amplify transposon-pTarget junctions ... -
Agilent
No products found because this supplier's products are not listed.bioRxiv - Genomics 2016, published in Genome Research doi: 10.1101/gr.213595.116Quote: ... A total of 60 libraries were created by PCR amplification with Illumina barcoding primers at 17 reaction cycles and quantified using a Bioanalyzer DNA 1000 kit (Agilent). Libraries were then pooled and sequenced on a HiSeq 2000 Sequencing system (Illumina). -
American BioInnovations,
No products found because this supplier's products are not listed.bioRxiv - Molecular Biology 2020Quote: ... TaqMan miRNA stem-loop RT primers/qPCR primer probe sets (ABI 4427975) (cel-miR-39 ID# 000200 Lot#P180110-003B10 ... -
Bioline
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2019Quote: ... The cDNA was then diluted 1:5 and qRT-PCR reactions were set up in triplicate using primers synthesized from IDT and SensiMix SYBR & Fluorescin kit (Bioline, Meridian Bioscience). qRT-PCR was done using a Corbett Research RG 6000 thermocycler ... -
Lucigen
No products found because this supplier's products are not listed.bioRxiv - Systems Biology 2019, published in npj Systems Biology and Applications doi: 10.1038/s41540-019-0121-4Quote: ... and indexed with ScriptSeq™ Index PCR primers set 1 (Epicentre, Illumina) following the standard protocol ... -
GE Life Sciences
No products found because this supplier's products are not listed.bioRxiv - Immunology 2020Quote: ... Whole blood was collected 31 and 32 days following a positive RT-PCR test and subjected to density gradient centrifugation using the Ficoll Paque Plus reagent (GE Healthcare, #17-1440-02). After separation ... -
3M
No products found because this supplier's products are not listed.bioRxiv - Cancer Biology 2019, published in PLOS ONE doi: 10.1371/journal.pone.0215696Quote: ... by overnight capillary transfer at RT using 20X SSC transfer buffer (3M NaCl, 0.3M Sodium citrate, pH 7). DNA was fixed on the membrane by UV-crosslinking at 1.2J/cm2 for 30s in HL-2000 Hybrilinker (UVP ... -
Addgene
No products found because this supplier's products are not listed.bioRxiv - Molecular Biology 2019Quote: ... the miR-141 indicator is from Addgene (#67632). To generate 3’UTR luciferase reporters for BCL6 ... -
BioLegend
No products found because this supplier's products are not listed.bioRxiv - Neuroscience 2019Quote: ... RT incubation in 2X SSC containing 8 µg/µl Brilliant Violet 421™ Streptavidin (BioLegend, Cat. No. 405225) and 0.05% Tween 20 ... -
Beckman
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2018, published in Nature Communications doi: 10.1038/s41467-018-06172-7Quote: ... and 17 clones were sent for Sanger sequencing with the M13_F primer (Beckman Coulter Genomics). -
Lonza
No products found because this supplier's products are not listed.bioRxiv - Cancer Biology 2020Quote: ... ITS (Lonza, 17-838Z), non-essenOal amino acids (Sigma ... -
Mirus Bio
No products found because this supplier's products are not listed.Cited in Evolution of hominin detoxification: Neanderthal and modern human AHR respond similarly to TCDDbioRxiv - Evolutionary Biology 2018Quote: ... HeLa cells were transfected using the TransIT-HeLaMONSTER Transfection Kit (MIR 2904) and HepG2 cells with TransIT-LT1 Transfection Reagent (MIR 2304) applying the standard protocols (both from Mirus Bio LLC ... -
Santa Cruz
No products found because this supplier's products are not listed.bioRxiv - Bioengineering 2019Quote: ... MART1and PFK transcripts were analyzed by SYBR Green–based real-time quantitative RT-PCR (qRT-PCR) using specific primers purchased from Santa Cruz. Data were normalized to the expression of RPL19 and are expressed as fold changes. -
Becton, Dickinson and Company
No products found because this supplier's products are not listed.bioRxiv - Immunology 2019Quote: ... IL-17-BV786 (TC11-18H10, BD biosciences), IL-22-PE (1H8PWSR ... -
TianGen Biotech
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2020Quote: ... Reverse transcription with ZIKA virus specific primer (Rev-AAGTGATCCATGTGATCAGTTGATCC) was performed using FastQuant RT Kit (Tiangen). Real-time PCR was done on 7900HT (ABI ... -
Eurogentec
No products found because this supplier's products are not listed.Cited in Mechanical activation of TRPV4 channels controls albumin reabsorption by proximal tubule cells.bioRxiv - Physiology 2019, published in Science Signaling doi: 10.1126/scisignal.abc6967Quote: ... cDNA-specific PCR primers were designed using Primer-BLAST (see Table 1) and purchased from Eurogentec (Seraing, Belgium). Gapdh was used as reference gene ... -
Eppendorf
No products found because this supplier's products are not listed.bioRxiv - Cancer Biology 2017, published in Nature Communications doi: 10.1038/s41467-017-01178-zQuote: ... cDNA was amplified by reverse transcriptase PCR (RT-PCR) using a Master Cycler Pro (Eppendorf, Hamburg, Germany) and visualized on a 1.5% agarose gel ... -
Calbiochem
No products found because this supplier's products are not listed.bioRxiv - Biophysics 2019, published in Proceedings of the National Academy of Sciences doi: 10.1073/pnas.2003184117Quote: ... 17 mg/mL Cellulysin (Calbiochem, La Jolla, CA), 17 mg/mL Cellulase RS (Yakult ... -
Vector Labs
No products found because this supplier's products are not listed.bioRxiv - Cancer Biology 2019, published in Scientific Reports doi: 10.1038/s41598-020-60269-yQuote: ... the cells were incubated with DAPI diluted in PBS for 10 min at RT and washed before being mounted onto slides with Vectashield Hard Set (Vector Laboratories). Single-planed images were taken on a Nikon C2 confocal microscope using a 40X oil-immersion lens ... -
Merck
No products found because this supplier's products are not listed.Cited in Interleukin-33 promotes type 1 cytokine expression via p38 MAPK in human natural killer cellsbioRxiv - Immunology 2019Quote: ... The inhibition of ADAM-17 (a disintegrin and metalloprotease-17) was performed by adding 8 μM TAPI-1 (Merck Millipore, Burlington, MA). TAPI-1 and MK2 IV were added 60 minutes before cytokine stimulation ... -
Chroma Technology
No products found because this supplier's products are not listed.bioRxiv - Bioengineering 2017, published in ACS Nano doi: 10.1021/acsnano.7b04743Quote: ... Fluorescence filter sets from Chroma Technology and Semrock were used ... -
VWR
No products found because this supplier's products are not listed.bioRxiv - Synthetic Biology 2020Quote: ... RT-PCR was performed with RedTaq Polymerase (VWR) with primers 51-Scorpine-F and 117-CP-ctrl-R on Sco-CP (1267bp) ... -
Macherey-Nagel GmbH
No products found because this supplier's products are not listed.bioRxiv - Plant Biology 2020Quote: ... RT-PCR products were purified using PCR Clean-up (Macherey-Nagel, 740609.250) following the manufacturer’s instructions ... -
R&D Systems
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2019, published in Frontiers in Cell and Developmental Biology doi: 10.3389/fcell.2019.00198Quote: ... IL-17 (R&D Systems) and TNF-α (Thermo Fisher ... -
abcam
No products found because this supplier's products are not listed.bioRxiv - Pathology 2020Quote: The serum IL-17 concentrations were quantified using a mouse IL-17 ELISA kit from Abcam (ab100702) according to the manufacturer’s instructions ... -
Research Products International
No products found because this supplier's products are not listed.bioRxiv - Genomics 2018Quote: ... separate PCR reactions were set up to generate the ADT library (SI-PCR and RPI-x primers), the hashtag library (SI-PCR and D7xx_s ... -
Larodan
No products found because this supplier's products are not listed.bioRxiv - Molecular Biology 2019, published in Environment International doi: 10.1016/j.envint.2020.105935Quote: ... triheptadecanoylglycerol (TG(17:0/17:0/17:0)) was purchased from Larodan AB (Solna ... -
Primerdesign
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2019, published in Nature Communications doi: 10.1038/s41467-019-12295-2Quote: ... and using DENV RT primer/probe Mix kit (Genesig, Primerdesign Ltd., UK) according to the manufacturer’s protocol ... -
Zymo Research
No products found because this supplier's products are not listed.bioRxiv - Neuroscience 2019Quote: Methylated and unmethylated DNA sets (Cat# D5017, pUC19 DNA set, Zymo research) were added as spike-in controls (2 ng spike-in control in 1 µg DNA ... -
Biosearch Technologies
No products found because this supplier's products are not listed.Cited in Stochastic transcription in the p53-mediated response to DNA damage is modulated by burst frequencybioRxiv - Systems Biology 2019, published in Molecular Systems Biology doi: 10.15252/msb.20199068Quote: Stellaris probe-sets for smFISH (Biosearch Technologies) were custom designed for intron and exon regions (see Appendix for oligo list ... -
Leica
No products found because this supplier's products are not listed.bioRxiv - Plant Biology 2018, published in PLOS ONE doi: 10.1371/journal.pone.0205117Quote: ... with the Ds-Red2 filter set (Leica). Induction of cDNA deletion was examined by genomic PCR using the primer sets 1 (MpEF-P_seqL1 and MpMPK1_c1131R_STP) ... -
PMT Corporation
No products found because this supplier's products are not listed.bioRxiv - Systems Biology 2019, published in Nature Microbiology doi: 10.1038/s41564-020-0704-2Quote: ... We calibrated the FSC and SSC gates to detect only yeast cells (FSC-PMT=681V, SSC-PMT=264V ... -
The Jackson Laboratory
No products found because this supplier's products are not listed.Cited in MicroRNA-223 protects neurons from degeneration in Experimental Autoimmune EncephalomyelitisbioRxiv - Neuroscience 2018, published in Brain doi: 10.1093/brain/awz245Quote: ... miR-223−/− and miR-223-/y KO mice (B6.Cg-Ptprca Mir223tm1Fcam/J) were purchased from Jackson Laboratories and maintained on a CD45.1+ Background (B6.SJL-Ptprca Pepcb/BoyJ ... -
Corning
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2019, published in eLife doi: 10.7554/eLife.49278Quote: ... then incubate in 2X SSC (Corning, cat#46-020-CM) for 5 min ... -
GenScript
No products found because this supplier's products are not listed.Cited in MicroRNA miR-1002 enhances NMNAT-mediated stress response by modulating alternative splicingbioRxiv - Neuroscience 2019, published in iScience doi: 10.1016/j.isci.2019.08.052Quote: ... miR-1002 negative scramble was ordered from Genscript for ligation into pAC-GAL4. -
Nikon
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2018, published in Journal of Cell Science doi: 10.1242/jcs.221556Quote: Fluorescence correlation spectroscopy (FCS) data sets were acquired on a custom-built set-up with an inverted Nikon Eclipse TE2000 microscope (Nikon) equipped with a 100x oil immersion objective (Nikon ... -
Evrogen
No products found because this supplier's products are not listed.bioRxiv - Plant Biology 2018, published in Genome Research doi: 10.1101/gr.253302.119Quote: ... The cDNA for RT-PCR was synthesized using an MMLV RT Kit (Evrogen, Russia) according to the manufacturer’s recommendations employing oligo(dT)17-primers from 2 µg total RNA after DNase treatment ... -
Origene Technologies
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2019Quote: ... and cloned into pCMV-miR (Origene). To generate replication-deficient adenovirus ... -
PerkinElmer
No products found because this supplier's products are not listed.bioRxiv - Molecular Biology 2019Quote: ... The RT-PCR were performed using AmpliTaq Gold DNA polymerase (Perkin Elmer, Boston, USA). PCR fragments were separated with electrophoresis using 1% agarose gel in tris-borate-EDTA (TBE ... -
Charles River Labs
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2018, published in Vaccine doi: 10.1016/j.vaccine.2019.05.022Quote: Eight-week-old female CB-17 SCID mice (Charles River Laboratories ... -
Olympus
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2018Quote: ... with a filter set (Olympus, U-MWU2 BP330~385). -
Carl Zeiss
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2019, published in Scientific Reports doi: 10.1038/s41598-020-58726-9Quote: ... For visualization of DAPI fluorescence filter set 49 (Carl Zeiss; excitation ...