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No products found
because this supplier's products are not listed.
Ilja Vietor, et al.,
bioRxiv - Cell Biology 2019
Quote:
... Quantitative RT-PCR was performed using Taqman probes and primer sets (Applied Biosystems) specific for CD36 (assay ID Mm00432398_m1) ...
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No products found
because this supplier's products are not listed.
Quote:
... and mir-34a (Qiagen MiScript primer assay). Real time PCR data was analyzed using the 2−▵▵Ct method24,25 and expressed as fold enrichment.
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No products found
because this supplier's products are not listed.
John A. Bennett, et al.,
bioRxiv - Pharmacology and Toxicology 2018
Quote:
... Prime PCR primer sets were purchased from Bio-Rad. Expression results were calculated by ΔΔCT method and were normalized to the reference genes GAPDH and βActin.
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No products found
because this supplier's products are not listed.
Prashanth Kumar Guthikonda, et al.,
bioRxiv - Developmental Biology 2017
Quote:
... Quantitative RT-PCR was set up with SYBR Premix Ex Taq II (Takara Bio) using specific RP gene primers (supplementary Table 1 ...
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No products found
because this supplier's products are not listed.
Quote:
... and indexed with ScriptSeq™ Index PCR primers set 1 (Epicentre, Illumina) following the standard protocol ...
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No products found
because this supplier's products are not listed.
Quote:
... RT-PCR performed using specific primers sets (Supplementary Tables 2, 3) and GoTaq® Green Master Mix (Promega, Madison, WI). (30-40 cycles ...
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No products found
because this supplier's products are not listed.
Quote:
... Primer probe sets were custom oligos (Sigma-Adrich) (Table 4).
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No products found
because this supplier's products are not listed.
Sway P. Chen, Harris H. Wang,
bioRxiv - Synthetic Biology 2019
Quote:
... We performed colony PCR using the primer set p898/p415 with KAPA2G Robust HotStart ReadyMix (Kapa Biosystems) to amplify transposon-pTarget junctions ...
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No products found
because this supplier's products are not listed.
Bingdong Liu, et al.,
bioRxiv - Microbiology 2019
Quote:
... The PCR product was indexed with Illumina sequencing primer-set by utilizing the Phusion HF Taq Polymerase (NEB), followed with gel purification ...
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No products found
because this supplier's products are not listed.
Keren Yizhak, et al.,
bioRxiv - Genomics 2018
Quote:
... PCR set-up and harvesting (Agilent Technologies, Lexington, MA). Indexed libraries (pools of amplicons ...
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No products found
because this supplier's products are not listed.
Bonita H. Powell, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... TaqMan miRNA stem-loop RT primers/qPCR primer probe sets (ABI 4427975) (cel-miR-39 ID# 000200 Lot#P180110-003B10 ...
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No products found
because this supplier's products are not listed.
CF Hung, et al.,
bioRxiv - Cell Biology 2019
Quote:
... The cDNA was then diluted 1:5 and qRT-PCR reactions were set up in triplicate using primers synthesized from IDT and SensiMix SYBR & Fluorescin kit (Bioline, Meridian Bioscience). qRT-PCR was done using a Corbett Research RG 6000 thermocycler ...
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No products found
because this supplier's products are not listed.
Quote:
... or 129S-Vimtm1Cba/MesDmarkJ (Vimentin KO) (Jackson Laboratory) were injected intravenously with 1 × 109 CFU of wild-type GBS or the isogenic ΔbspC mutant for a high dose challenge ...
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No products found
because this supplier's products are not listed.
Quote:
... and indexed with ScriptSeq™ Index PCR primers set 1 (Epicentre, Illumina) following the standard protocol ...
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No products found
because this supplier's products are not listed.
Jac M.M.J.G. Aarts, et al.,
bioRxiv - Evolutionary Biology 2018
Quote:
... HeLa cells were transfected using the TransIT-HeLaMONSTER Transfection Kit (MIR 2904) and HepG2 cells with TransIT-LT1 Transfection Reagent (MIR 2304) applying the standard protocols (both from Mirus Bio LLC ...
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No products found
because this supplier's products are not listed.
Yan Chen, et al.,
bioRxiv - Molecular Biology 2019
Quote:
... the miR-141 indicator is from Addgene (#67632). To generate 3’UTR luciferase reporters for BCL6 ...
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No products found
because this supplier's products are not listed.
Zhihua Liu, et al.,
bioRxiv - Microbiology 2020
Quote:
... Reverse transcription with ZIKA virus specific primer (Rev-AAGTGATCCATGTGATCAGTTGATCC) was performed using FastQuant RT Kit (Tiangen). Real-time PCR was done on 7900HT (ABI ...
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No products found
because this supplier's products are not listed.
Quote:
... cDNA-specific PCR primers were designed using Primer-BLAST (see Table 1) and purchased from Eurogentec (Seraing, Belgium). Gapdh was used as reference gene ...
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No products found
because this supplier's products are not listed.
Quote:
... cDNA was amplified by reverse transcriptase PCR (RT-PCR) using a Master Cycler Pro (Eppendorf, Hamburg, Germany) and visualized on a 1.5% agarose gel ...
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No products found
because this supplier's products are not listed.
Justin Rendleman, et al.,
bioRxiv - Systems Biology 2018
Quote:
... in a 50 μl volume using specific oligos (Truseq Riboprofile Forward PCR primer and Index PCR primer) and purified the samples with Agencount AMPure beads (Beckman Coulter). We purified the amplified libraries by using 8% Native PAGE and verified the final library size using the High Sensitivity DNA assay on the Agilent Bioanalyzer ...
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No products found
because this supplier's products are not listed.
Giuseppina D’Alessandro, et al.,
bioRxiv - Cell Biology 2018
Quote:
... protease inhibitor cocktail set III (Calbiochem) and Benzonase 1:1000 (Sigma ...
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No products found
because this supplier's products are not listed.
Quote:
... transcription factor buffer set (BD Bioscience) was used according to the manufacturer’s instructions ...
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No products found
because this supplier's products are not listed.
Quote:
... with 100nM miR scrambled or miR-181a mimic (100nM; GE Healthcare) (Soriano-Arroquia et al. ...
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No products found
because this supplier's products are not listed.
Quote:
... Fluorescence filter sets from Chroma Technology and Semrock were used ...
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No products found
because this supplier's products are not listed.
Yapeng Su, et al.,
bioRxiv - Bioengineering 2019
Quote:
... MART1and PFK transcripts were analyzed by SYBR Green–based real-time quantitative RT-PCR (qRT-PCR) using specific primers purchased from Santa Cruz. Data were normalized to the expression of RPL19 and are expressed as fold changes.
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No products found
because this supplier's products are not listed.
Pastor Jullian Fabres, et al.,
bioRxiv - Plant Biology 2020
Quote:
... RT-PCR products were purified using PCR Clean-up (Macherey-Nagel, 740609.250) following the manufacturer’s instructions ...
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No products found
because this supplier's products are not listed.
Eleni Mimitou, et al.,
bioRxiv - Genomics 2018
Quote:
... separate PCR reactions were set up to generate the ADT library (SI-PCR and RPI-x primers), the hashtag library (SI-PCR and D7xx_s ...
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No products found
because this supplier's products are not listed.
Quote:
Male 10 weeks old 129/SV mice (Charles River, Germany) were held in individually ventilated cages and received a standard diet with free access to tap water ...
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No products found
because this supplier's products are not listed.
Quote:
... and using DENV RT primer/probe Mix kit (Genesig, Primerdesign Ltd., UK) according to the manufacturer’s protocol ...
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No products found
because this supplier's products are not listed.
Quote:
... miR-1002 negative scramble was ordered from Genscript for ligation into pAC-GAL4.
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No products found
because this supplier's products are not listed.
Quote:
... the cells were incubated with DAPI diluted in PBS for 10 min at RT and washed before being mounted onto slides with Vectashield Hard Set (Vector Laboratories). Single-planed images were taken on a Nikon C2 confocal microscope using a 40X oil-immersion lens ...
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No products found
because this supplier's products are not listed.
Dennis Diaz, et al.,
bioRxiv - Synthetic Biology 2020
Quote:
... Gene insertion was verified by PCR using the primer pairs pETUpstream/DuetDOWN or DuetUP2/T7 Terminator (Merck) with Enc or mSOG-containing plasmids as template DNA ...
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No products found
because this supplier's products are not listed.
Anne-Laure Mahul-Mellier, et al.,
bioRxiv - Neuroscience 2018
Quote:
... phospho-serine 129 α-syn (1:1,500, Abcam), actin (1:3,000 ...
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No products found
because this supplier's products are not listed.
Quote:
... The cDNA for RT-PCR was synthesized using an MMLV RT Kit (Evrogen, Russia) according to the manufacturer’s recommendations employing oligo(dT)17-primers from 2 µg total RNA after DNase treatment ...
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No products found
because this supplier's products are not listed.
Nicholas O’Toole, et al.,
bioRxiv - Neuroscience 2019
Quote:
Methylated and unmethylated DNA sets (Cat# D5017, pUC19 DNA set, Zymo research) were added as spike-in controls (2 ng spike-in control in 1 µg DNA ...
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No products found
because this supplier's products are not listed.
Quote:
... the ELISA MAXTM Deluxe Set (Biolegend) was used according to the manufacturer’s instructions ...
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No products found
because this supplier's products are not listed.
Quote:
... with the Ds-Red2 filter set (Leica). Induction of cDNA deletion was examined by genomic PCR using the primer sets 1 (MpEF-P_seqL1 and MpMPK1_c1131R_STP) ...
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No products found
because this supplier's products are not listed.
Quote:
Fluorescence correlation spectroscopy (FCS) data sets were acquired on a custom-built set-up with an inverted Nikon Eclipse TE2000 microscope (Nikon) equipped with a 100x oil immersion objective (Nikon ...
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No products found
because this supplier's products are not listed.
Yasuhiko Chikami, et al.,
bioRxiv - Molecular Biology 2019
Quote:
... The RT-PCR were performed using AmpliTaq Gold DNA polymerase (Perkin Elmer, Boston, USA). PCR fragments were separated with electrophoresis using 1% agarose gel in tris-borate-EDTA (TBE ...
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No products found
because this supplier's products are not listed.
Quote:
Stellaris probe-sets for smFISH (Biosearch Technologies) were custom designed for intron and exon regions (see Appendix for oligo list ...
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No products found
because this supplier's products are not listed.
Syun-Wun Liang, et al.,
bioRxiv - Microbiology 2018
Quote:
... with a filter set (Olympus, U-MWU2 BP330~385).
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No products found
because this supplier's products are not listed.
Quote:
... For visualization of DAPI fluorescence filter set 49 (Carl Zeiss; excitation ...
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No products found
because this supplier's products are not listed.
Kirsty E Waddington, et al.,
bioRxiv - Immunology 2019
Quote:
... Human Negative Control Primer Set 1 (Active Motif, 71001) was used as a negative control.
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No products found
because this supplier's products are not listed.
Hera Canaj, et al.,
bioRxiv - Genomics 2019
Quote:
... PCR primers were ordered from IDT DNA either unmodified (“5’ OH” ...
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No products found
because this supplier's products are not listed.
Astrid Hoermann, et al.,
bioRxiv - Synthetic Biology 2020
Quote:
... RT-PCR was performed with RedTaq Polymerase (VWR) with primers 51-Scorpine-F and 117-CP-ctrl-R on Sco-CP (1267bp) ...
-
No products found
because this supplier's products are not listed.
Quote:
... RT-PCR was performed using the Maxime PCR Premix Kit (Intron Biotechnology, Seoul, Korea). Transcription of HC and LC genes was confirmed with primers as described above ...
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No products found
because this supplier's products are not listed.
Tyler J. Marquart, et al.,
bioRxiv - Cell Biology 2019
Quote:
... and cloned into pCMV-miR (Origene). To generate replication-deficient adenovirus ...
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No products found
because this supplier's products are not listed.
Lianna Fung, et al.,
bioRxiv - Cell Biology 2017
Quote:
... Amount of hnRNPA1 mRNA was analyzed by qRT-PCR (Sense primer 5’-aagcaattttggaggtggtg-3’; Antisense primer 5’-atagccaccttggtttcgtg-3’) using Forget-me-not qPCR mastermix (Biotium) relative to beta-2-microglobulin (B2m ...
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No products found
because this supplier's products are not listed.
Quote:
... The PCR products were sequenced using Sanger sequencing with the same primers used for the PCR (Eton Bioscience).
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No products found
because this supplier's products are not listed.
Cailu Song, et al.,
bioRxiv - Cancer Biology 2017
Quote:
Recombinant lentivirus vectors encoding miR-200c (miR-200c) or negative control (Control) (pEZX-MR03, HIV based, GeneCopoeia, USA) were used for transient or stable overexpression of miR-200c ...