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No products found
because this supplier's products are not listed.
Jooyoung Lee, et al.,
bioRxiv - Bioengineering 2019
Quote:
... 500 ng of each Acr vector and GFP plasmid used as a transfection control were transfected in a 6-well format using Lipofectamine 2000 (Invitrogen). The total DNA amount was kept constant by adding a stuffer plasmid in all cases.
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No products found
because this supplier's products are not listed.
Charlotte M. de Winde, et al.,
bioRxiv - Cell Biology 2020
Quote:
... NL) or GFP control plasmid (0.5μg DNA per plasmid) using Attractene transfection reagent (Qiagen) as per supplier’s instructions ...
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No products found
because this supplier's products are not listed.
Suha Naffar-Abu Amara, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... shRNA-GFP control (Addgene, #30323).
-
No products found
because this supplier's products are not listed.
Quote:
... Virus-like particles (VLPs) containing the GFP control or targeting sgRNAs were generated by transfection of HEK293 cells with FuGene (Promega). VLPs were collected from culture supernatant ...
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No products found
because this supplier's products are not listed.
Joshua E. Lewis, et al.,
bioRxiv - Systems Biology 2020
Quote:
... Negative controls consisted of transfection with the MISSION siRNA Universal Negative Control (Sigma-Aldrich; Cat#SIC001; Lot#WDAA1199). Predesigned MISSION siRNA’s targeting individual genes were ordered from Sigma-Aldrich ...
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No products found
because this supplier's products are not listed.
Quote:
... Transient transfection of GFP and GFP-D1 was performed using Fugene HD (Roche) reagent according to the manufacturer’s protocol.
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No products found
because this supplier's products are not listed.
Quote:
... GFP+ and GFP- cells were sorted 24h post transfection using a FACSAria Cell Sorter (BD Biosciences) to a purity of >98% ...
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No products found
because this supplier's products are not listed.
Domokos I. Lauko, et al.,
bioRxiv - Cell Biology 2020
Quote:
... Cells were transfected with 5 μg of pACT-GFP-actin using TransIT-Insect transfection reagent (Mirus Bio) and incubated for 2 d at 28°C in Grace’s media with 10% FBS and antibiotics (100 μg/ml penicillin/streptomycin and 0.25 μg/ml Amphotericin B) ...
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No products found
because this supplier's products are not listed.
Quote:
... lysates were incubated with GFP-Trap® or control (ChromoTek) magnetic agarose beads for 1-2 hr at 4°C with end-to-end rotation ...
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No products found
because this supplier's products are not listed.
Quote:
... The plasmids generated by site-directed mutagenesis were verified by DNA sequencing and the transfection efficiency was assessed by co-transfection with a green fluorescent protein (GFP) expression plasmid (pEGFP-C1, Clontech).
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No products found
because this supplier's products are not listed.
Quote:
... and against GFP as a negative control (Santa Cruz, sc-8334). Cross-linking ...
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No products found
because this supplier's products are not listed.
Mengsha Li, et al.,
bioRxiv - Developmental Biology 2020
Quote:
... The transfection efficiency of AAV9 was determined by staining with an anti-GFP antibody (Abcam, ab13970). In addition ...
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No products found
because this supplier's products are not listed.
Daria Esterházy, et al.,
bioRxiv - Immunology 2018
Quote:
... anti-GFP (Aves Labs, GFP-1020); anti-OVA IgG1 ...
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No products found
because this supplier's products are not listed.
Quote:
... or the isotype control (Rat IgG1 Κ Isotype Control, Biolegend) through i.p ...
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No products found
because this supplier's products are not listed.
Quote:
... Transfection efficiency was determined by transfecting the pMAX-GFP (Lonza) and subsequent FACS analysis ...
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No products found
because this supplier's products are not listed.
Lianna Li, Kierra Jones, Hao Mei,
bioRxiv - Cancer Biology 2019
Quote:
... Control HCT116 cells (WT) were established by transfecting pCMV6-AC-GFP Tagged Cloning Vector (Origene, Cat # PS100010) into HCT116 cells ...
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No products found
because this supplier's products are not listed.
Roza H. Ali Masalmeh, et al.,
bioRxiv - Genetics 2019
Quote:
... 10% PhiX control library (Illumina v3 control library) was spiked in to facilitate sequencing by generating additional sequence diversity ...
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No products found
because this supplier's products are not listed.
Alexandra Surcel, et al.,
bioRxiv - Cancer Biology 2017
Quote:
... and GFP control were purchased from Vector BioLabs, Malvern ...
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No products found
because this supplier's products are not listed.
Alexey Stukalov, et al.,
bioRxiv - Systems Biology 2020
Quote:
... Firefly reporter and Renilla transfection control were transfected together with plasmids expressing viral proteins using polyethylenimine (PEI, Polysciences) for untreated and treated conditions ...
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No products found
because this supplier's products are not listed.
Joshua E. Lewis, et al.,
bioRxiv - Systems Biology 2020
Quote:
... Figure S5 shows the knockdown efficiency of siRNA transfections in each cell line with GAPDH siRNA compared to no siRNA (just N-TER transfection reagent) and negative control siRNA by Western blot (GAPDH antibody: Cell Signaling Technology, Cat#2118 ...
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No products found
because this supplier's products are not listed.
Quote:
... control mice received an irrelevant isotype control antibody (LTF2; BioXCell).
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No products found
because this supplier's products are not listed.
Sydney M. Sanderson, et al.,
bioRxiv - Molecular Biology 2018
Quote:
... or GFP control (Genecopoeia, EX-EGFP-Lv105) plasmid ...
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No products found
because this supplier's products are not listed.
Dennis Awuah, et al.,
bioRxiv - Immunology 2020
Quote:
... Transfection efficiency was determined using the fluorescently-labelled siGLO RISC-free control siRNA (GE Healthcare) and miRNA and/or mRNA expression was assessed afterwards.
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No products found
because this supplier's products are not listed.
Chia-En Wong, Kuen-Jer Tsai,
bioRxiv - Neuroscience 2019
Quote:
... HEK293 cells were transfected with GFP-TDP-43 by PolyJet™ DNA in vitro transfection reagent (Signagen Laboratories). Cells were seeded into a 6-well plate at a density of 5 × 105 cells/well and incubated overnight ...
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No products found
because this supplier's products are not listed.
Quote:
... Constructs of Kmpv1 and KmpvSP1 were transiently expressed as fusion proteins with GFP on the C-terminus using the liposomal transfection reagent GeneJuice® (MERCK KGaA, Darmstadt, Germany). Measurements were performed at room temperature in a bath solution containing ...
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No products found
because this supplier's products are not listed.
Quote:
... GFP (Rockland Immunochemicals ...
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No products found
because this supplier's products are not listed.
Denner S. Anjos, et al.,
bioRxiv - Cancer Biology 2019
Quote:
Negative controls were run for all antibodies with a mouse universal negative control (Dako, Carpinteria, CA, USA) according to the manufacturer’s instructions ...
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No products found
because this supplier's products are not listed.
Quote:
Manual quantification of lysosome size and imaging of GFP-based transfections were imaged using a Leica DM5000B system outfitted with a DFC350FX camera and controlled by Leica application suite (Leica Microsystems Inc. ...
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No products found
because this supplier's products are not listed.
Quote:
40 µl of electrocompetent cells were transformed with 1 ng of the lesion-containing or lesion-free control plasmids together with 1 ng of pVP146 (transformation control) by electroporation (GenePulser Xcell(tm) from BioRad, 2.5kV – 25µF – 200Ω) ...
-
No products found
because this supplier's products are not listed.
Quote:
... mature schizonts from the DPAP3-GFP and 3D7 control lines were concentrated using a magnetic activated cell sorting (MACS) LD separation column (Miltenyi Biotec). Briefly ...
-
No products found
because this supplier's products are not listed.
Quote:
... GFP filter set (Nikon Epi-Fl Filter Cube GFP-B), computer controlled shutters ...
-
No products found
because this supplier's products are not listed.
Danielle Kerkhofs, et al.,
bioRxiv - Neuroscience 2020
Quote:
... Cx3Cr1GFP/GFP mice (Jackson Lab 005582) were crossed with Thy-1YFP/0 mice (Jackson Lab 003782 ...
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No products found
because this supplier's products are not listed.
Mahesh Rao, Dominic Didiano, James G. Patton,
bioRxiv - Neuroscience 2016
Quote:
... GFP (Torrey Pines BioLabs, TP401), mCherry (Novus Biologicals ...
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No products found
because this supplier's products are not listed.
Quote:
... the stable GFP-tau-RFP cells and GFP-32AA-RFP control cells were expanded in two 225 cm2 flasks (Corning). The preparations for tau-GFP/RFP FRET biosensors and the soluble GFP/RFP controls cells were carried out similar as above.
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No products found
because this supplier's products are not listed.
Quote:
... GFP expression is determined 1 day after transfection using a fluorescence microscope (Olympus CKX53).
-
No products found
because this supplier's products are not listed.
Quote:
... coli DNA control (Zymo) in 150 μl water was sheared into ~10 kb fragments using g-Tube (Covaris ...
-
No products found
because this supplier's products are not listed.
Ji Young Kim, et al.,
bioRxiv - Cell Biology 2020
Quote:
... and a scrambled control peptide (GenScript) were used for addback experiments ...
-
No products found
because this supplier's products are not listed.
Quote:
... under control of pCLAMP10.3 (Molecular Devices). Patch-clamp recording pipettes with a resistance of 5–8 MΩ were filled with solution containing 128 mm K-gluconate ...
-
No products found
because this supplier's products are not listed.
Quote:
Detection of apoptotic TCam-2 cells was performed 48 h after transfection of TCam-2 cells with control and KIF18A siRNA constructs using an Annexin V-FITC Apoptosis Detection Kit (Beckman Coulter), according to the manufacturer’s protocol ...
-
No products found
because this supplier's products are not listed.
Quote:
... using jetPRIME transfection system (Polyplus-transfection, VWR 89129-922). RT-qPCR was conducted using the Power SYBR Green RNA-to-CT™ 1-Step Kit (Thermo-Fisher Scientific) ...
-
No products found
because this supplier's products are not listed.
Quote:
... κ Isotype Control from R&D System. Red blood cell lysis was performed using Versalyse lysing solution (Beckman Coulter) ...
-
No products found
because this supplier's products are not listed.
Quote:
... GFP-MyoXΔPH and GFP-MyoXΔFERM were generated with Q5 Site-Directed Mutagenesis Kit (New England Biolabs, E0554S), where the amino acids (1212-1253 ...
-
No products found
because this supplier's products are not listed.
Candice B. Herber, et al.,
bioRxiv - Physiology 2018
Quote:
... GFP staining used anti-GFP antibody (Novus Biologicals, Littleton, CO) at 1:2500 ...
-
No products found
because this supplier's products are not listed.
Quote:
... primary keratinocytes isolated from H2B-GFP epidermal Par3KO (K14CreneoKIwt/+;Par3fl/fl;H2B-GFP40) and control mice (Par3fl/fl;H2B-GFP) were seeded in μ-Slides (8 Well Glass Bottom ibiTreat; IBIDI) and calcium-switched for 24 hours ...
-
No products found
because this supplier's products are not listed.
Kitti D Csalyi, et al.,
bioRxiv - Developmental Biology 2019
Quote:
... anti-GFP (GeneTex) 1:4000 ...
-
No products found
because this supplier's products are not listed.
Misaki Matsui, et al.,
bioRxiv - Cell Biology 2019
Quote:
... The U2OS USP42 KO cell line and its complemented cell lines stably expressing either GFP or GFP-USP42 (FL, ΔC or Δ946-1196) were cultured with identical media with 1 μg/ml puromycin (InvivoGen) or with 1 μg/ml puromycin and 500 μg/ml Geneticin ...
-
No products found
because this supplier's products are not listed.
N. O. Alieva, et al.,
bioRxiv - Cell Biology 2018
Quote:
... and Volocity control software (PerkinElmer).
-
No products found
because this supplier's products are not listed.
Shira Weingarten-Gabbay, et al.,
bioRxiv - Genomics 2017
Quote:
... in a monochromator (Tecan i-control), and the samples were mixed in ratios corresponding to their ratio in the population ...
-
No products found
because this supplier's products are not listed.
Quote:
... and floated on top of 200 µL media (control or control media with 100ng/ml FGF2) in 50 mm glass-bottom dishes (MatTek P50G-1.5-14F) for 7 days.
-
No products found
because this supplier's products are not listed.
Krisztina Ötvös, et al.,
bioRxiv - Plant Biology 2019
Quote:
... GFP fluorescence intensities captured by ZEN software (Carl Zeiss, Germany) were measured and quantified by ImageJ software (http://imagej.nih.gov/ij/).
