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No products found
because this supplier's products are not listed.
Eric Weh, et al.,
bioRxiv - Genetics 2019
Quote:
The relative abundance of the two splice isoforms of pitx2 (pitx2a and pitx2c) were measured using PowerUp Sybr Green Master Mix (Thermo Fisher Scientific, Waltham, MA, USA) and a BioRad CFX 96 or 384 Real-Time PCR Detection System (Bio-Rad) ...
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No products found
because this supplier's products are not listed.
Quote:
... Tau3R and Tau4R - mouse monoclonal anti-Tau RD3 (3-repeat isoform) and mouse monoclonal anti-Tau RD4 (4-repeat isoform) were obtained from Millipore Corporation (Billerica ...
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No products found
because this supplier's products are not listed.
Quote:
... human intact PTH (Abcam), soluble RANKL (Immundiagnostik ...
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No products found
because this supplier's products are not listed.
Quote:
... Removal of the native splice acceptor in the 3’ homologous arm of HDR templates was achieved via site-directed mutagenesis (Agilent Technologies, 200523).
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No products found
because this supplier's products are not listed.
Shaobin Guo, et al.,
bioRxiv - Bioengineering 2017
Quote:
... Linear DNAs were made by PCRing protein expression related sequences out of GGA constructs using Phusion Hot Start Flex 2X Master Mix (M0536L) from NEB.
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No products found
because this supplier's products are not listed.
Annika Treyball, et al.,
bioRxiv - Physiology 2020
Quote:
... PTH (H-1660, Bachem) was aliquoted and stored in glass vials at −70°C as a 10−4 M stock in 4 mM HCl supplemented with 0.1% bovine serum albumin ...
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No products found
because this supplier's products are not listed.
Sanghyeon Lee, et al.,
bioRxiv - Neuroscience 2019
Quote:
... control non-related target knockdown (5′-AGTGGATTCGAG-AGCGTGT-3′) (GenScript). To produce lentiviral particles ...
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No products found
because this supplier's products are not listed.
Elena Ambrosetti, et al.,
bioRxiv - Bioengineering 2019
Quote:
Biacore T200 instrument and related reagents (GE Healthcare) were used to perform all Surface Plasmon Resonance experiments ...
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No products found
because this supplier's products are not listed.
Cemalettin Bekpen, et al.,
bioRxiv - Evolutionary Biology 2017
Quote:
Morpheus splice variants were detected by a quantitative PCR assay using the LightCycler SYBR Green System (Roche) with exon 6 and 8 primers (see Supplemental Table 2 for a complete list of oligonucleotides used) ...
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No products found
because this supplier's products are not listed.
Frances Anne Tosto, et al.,
bioRxiv - Molecular Biology 2018
Quote:
... Quantification of mRNA isoforms was performed using Image Lab 5.2.1 software (Bio-Rad).
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No products found
because this supplier's products are not listed.
Yashar Sadigh, et al.,
bioRxiv - Genomics 2019
Quote:
... splice junction amplicons were cloned in pGEM-T plasmid (Promega) and subjected to Sanger sequencing ...
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No products found
because this supplier's products are not listed.
Quote:
... All miRNA-related reagents were purchased from Qiagen.
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No products found
because this supplier's products are not listed.
Quote:
... 10 ng/ml Dickkopf-related protein 1 (DKK1, Peprotech), 10 ng/ml Insulin Growth Factor 1 (IGF1 ...
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No products found
because this supplier's products are not listed.
Marina García-Macia, et al.,
bioRxiv - Cell Biology 2019
Quote:
... retinoid-related orphan receptor alpha (RORα) and melatonin receptor 1A (MT1) from Santa Cruz Biotechnology (Santa Cruz ...
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No products found
because this supplier's products are not listed.
LaCarubba Brianna, et al.,
bioRxiv - Neuroscience 2019
Quote:
Δe11- and +e11-Cacna1d splice variants were transiently expressed in human tsA201 cells with CaVβ3 (Addgene, 26574), CaVα2δ-1 (Addgene ...
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No products found
because this supplier's products are not listed.
Quote:
... All isoforms of PKC were inhibited using 5 μΜ GF 109203X (Tocris; at lower concentrations GF190203X preferentially inhibits the -α and -β isoforms ...
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No products found
because this supplier's products are not listed.
Quote:
... Pre-cleared sample was mixed with an anti-SynGAP antibody recognizing an epitope common to all its isoforms (#5540S; Cell Signaling) at a 1:15 (v:v ...
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No products found
because this supplier's products are not listed.
Quote:
Mouse Mecp2 cDNA (e2 isoform) was cloned into the vector pEGFP-C1 (Clontech) to create in-frame fusions with an N-terminal EGFP tag ...
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No products found
because this supplier's products are not listed.
Quote:
... Base errors in the polished isoforms were further corrected by Illumina RNA-Seq reads using LoRDEC v0.8 (Salmela and Rivals ...
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No products found
because this supplier's products are not listed.
Quote:
DMS-53 cells transduced with DARPP-32 shRNAs or exogenously overexpressed DARPP-32 isoforms were stained with Annexin V-FITC antibodies (BD Biosciences) to assess apoptosis ...
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No products found
because this supplier's products are not listed.
Quote:
The human cytochrome isoforms were purchased from Corning unless CYP2C19 that was purchased from Cypex ...
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No products found
because this supplier's products are not listed.
Xiang Chen, et al.,
bioRxiv - Genetics 2018
Quote:
... for 15min and then treated with PTH(1-34) (50 nM) (R&D Systems, Inc., Minneapolis, USA) for 20 min ...
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No products found
because this supplier's products are not listed.
Barbara Mair, et al.,
bioRxiv - Genomics 2019
Quote:
... or CC2C6 (for pyro-Glu modified protein; BioLegend, Cat. #323123/4, RRID: AB_2716202/3) anti-CD47 antibodies were used with Protein-G DynabeadsTM or Biotin-binder DynabeadsTM ...
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No products found
because this supplier's products are not listed.
Quote:
... Eluted proteins were dialyzed using a 3 kDa membrane D-Tube Dialyzer (Merck, Germany) to remove the imidazole in Storage Buffer (200 mM ammonium acetate ...
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No products found
because this supplier's products are not listed.
Tao Guo, et al.,
bioRxiv - Plant Biology 2019
Quote:
... The purified Arp2/3 protein complex from porcine brain (Cytoskeleton) and GST-tagged VCA domain of human WASP protein (Cytoskeleton ...
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No products found
because this supplier's products are not listed.
Quote:
... Y1-3 proteins (25 nM) and acceptor beads (catalog # AL110C, 1:100 dilution; PerkinElmer) were mixed and adjusted to 30 µL ...
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No products found
because this supplier's products are not listed.
Quote:
... 1,2-dipalmitoyl-sn-glycero-3-phosphocholine and 1-palmitoyl-2-oleoyl-sn-3-phosphoethanolamine (3:1; Avanti Polar Lipids) were dissolved in chloroform ...
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No products found
because this supplier's products are not listed.
Quote:
... The splices were photographed using a BX60 light microscope (Olympus, Japan).
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No products found
because this supplier's products are not listed.
Quote:
... Insoluble protein pellets were resolubilized in 3 M urea (VWR, Radnor, PA) and purified using immobilized metal affinity chromatography using Ni-NTA resin (Qiagen ...
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No products found
because this supplier's products are not listed.
Arthur Charles-Orszag, et al.,
bioRxiv - Cell Biology 2018
Quote:
... or the Carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1, kindly provided by Alain Servin) with the Amaxa Nucleofector device (Amaxa Biosystem, Lonza).
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No products found
because this supplier's products are not listed.
Quote:
... 3-PO (Calbiochem), L-aspartic acid (Sigma) ...
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![3-Chloro-4-(pyridin-3-yl)-1,2,5-thiadiazole - CAS [131986-28-2], >99%](https://image.labshake.com/supplier-product/305cc4ba-a97e-4815-88b6-a95430b03ef4/47d20e4b-8fae-4b42-af7a-381814fbe0f6.png)
Building block for the synthesis of FP-TZTP.
Sold for research purposes only.
Cat# 2592.0, SKU# 2592-1000,
1000 mg, US $137 / EA, EURO €125 / EA
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Quote:
... CHIR99021 (3 μM; Axon Medchem), PD0325901 (PD ...
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No products found
because this supplier's products are not listed.
Pouyan Asgharzadeh, et al.,
bioRxiv - Bioengineering 2020
Quote:
... 21 FtsZ2-1 and 16 FtsZ1-2 isoforms) were taken with a Leica TCS SP8 microscope (Leica Microsystems, Wetzlar, Germany), using the HCX PL APO 100x/1.40 oil objective and applying the microscopy conditions described previously [24 ...
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No products found
because this supplier's products are not listed.
Quote:
... anti-Band 3 and anti-protein 4.2 (kind gifts from Connie Birkenmeier, Jackson Laboratory, US).
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No products found
because this supplier's products are not listed.
Tamara Weiss, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... Protein extracts were stored in Protein LoBind tubes (Eppendorf) at −80°C ...
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No products found
because this supplier's products are not listed.
Emma J Kelsall, et al.,
bioRxiv - Molecular Biology 2019
Quote:
... Protein samples were run on a SDS-PAGE gel and proteins were visualised by InstantBlue protein stain (Expedeon). Quantitation of the intensities of Ras1.G17V (1-172 ...
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No products found
because this supplier's products are not listed.
Zhuoling An, Chen Shi, Pengfei Li, Lihong Liu,
bioRxiv - Biophysics 2020
Quote:
The derivatization of 44 amino acids and related amines were conducted on the iTRAQ® Reagent Kit 200 Assay (P/N: A1116, AB Sciex, USA), including phosphoserine (PSer) ...
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No products found
because this supplier's products are not listed.
Sunyuan Zhang, et al.,
bioRxiv - Biochemistry 2018
Quote:
... whole cell lysates prepared from Neuro-2a cells expressing GFP fusion constructs (3 mg protein) were incubated with GFP-trap-agarose (0.05 mL) (ChromoTek). The incubation for streptavidin-agarose was overnight at 4ºC ...
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No products found
because this supplier's products are not listed.
Marc-Benjamin Aurin, et al.,
bioRxiv - Plant Biology 2019
Quote:
... The protein without His-Tag was concentrated using a Vivaspin 20 Ultrafiltration Unit (3 kDa molecular weight cutoff, Sartorius, www.sartorius.de). An appropriate volume was applied to a 1 ml loop of a gel filtration column (Superdex75 10/300 GL ...
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No products found
because this supplier's products are not listed.
Quote:
... 3′-diaminobenzidine (Vector Laboratories), counterstained with hematoxylin ...
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No products found
because this supplier's products are not listed.
Shigeo Takashima, et al.,
bioRxiv - Biochemistry 2020
Quote:
... 15-octadecatrienoic acid (C18:3 ω-3, α-linolenic acid; Cayman Chemical, #90210), all cis-6 ...
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No products found
because this supplier's products are not listed.
May Daher, et al.,
bioRxiv - Immunology 2020
Quote:
... Cas9 protein (PNA bio) and gRNA were incubated at room temperature for 15 min in a 3:1 reaction with 15μg Cas9 and 5000 ng gRNA (2500ng of sgRNA#1 and 2500ng of sgRNA#2).47 The incubation product (gRNA and Cas9 complex ...
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No products found
because this supplier's products are not listed.
Nadiia Rawlings, et al.,
bioRxiv - Cell Biology 2019
Quote:
... Total protein on membranes was stained using REVERT total protein stain (LI-COR). Immune complexes were detected using HRP-conjugated secondary antibodies (Sigma ...
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No products found
because this supplier's products are not listed.
Yu Cao, et al.,
bioRxiv - Developmental Biology 2019
Quote:
... The related secondary antibodies were purchased from BOSTER Technology ...
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No products found
because this supplier's products are not listed.
Suvadip Mallick, et al.,
bioRxiv - Microbiology 2019
Quote:
... JEV non-structural protein 3 (Genetex; 1:10000), mouse cleaved-Caspase 3 (Cell Signaling ...
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No products found
because this supplier's products are not listed.
Quote:
... For genes related to spatial distribution ISH was performed on tissue sections from wild type mouse brains (C57BL/6, Charles River). Adult mice were transcardially perfused with 1x PBS followed by 4% paraformaldehyde and post-fixation in 4%PFA overnight ...
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No products found
because this supplier's products are not listed.
Chun-Yen Chen, et al.,
bioRxiv - Plant Biology 2018
Quote:
... Purified protein concentrations were determined at 595 nm absorbance using an ELISA reader (FlexStation 3, Molecular Devices) and the standard curve method ...
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No products found
because this supplier's products are not listed.
Alisa F. Zyryanova, et al.,
bioRxiv - Molecular Biology 2017
Quote:
3 μL aliquots of the protein complex was applied on glow-discharged holey carbon grids (Quantifoil R2/2). Grids were blotted and flash-frozen in liquid ethane using a Vitrobot automat (Thermo Fisher) ...
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No products found
because this supplier's products are not listed.
Quote:
H3K27me2/3 ChIP using anti-H3K27me2/3 antibody (Active Motif, 39536) was performed as previously described (12 ...
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No products found
because this supplier's products are not listed.
Justin Parreno, et al.,
bioRxiv - Cell Biology 2019
Quote:
... Total protein of samples loaded into capillaries was determined using a Total Protein Separation module kit (Protein Simple). Protein expression of specific molecules were normalized to total protein.