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No products found
because this supplier's products are not listed.
Quote:
... a blocking FLAG peptide (Sigma), used at a 100 μg/ml concentration ...
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No products found
because this supplier's products are not listed.
Fengchun Liu, et al.,
bioRxiv - Genomics 2018
Quote:
... followed by blocking in blocking reagent (Roche, #11175041910). The bound riboprobe was detected after incubation of trematodes in antidigoxigenin alkaline phosphatase-conjugated antibody (Roche ...
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No products found
because this supplier's products are not listed.
Quote:
... numbers of surviving p14 T cells were subsequently calculated using Countess (Invitrogen) or Vi-Cell (Beckmann Coulter ...
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No products found
because this supplier's products are not listed.
Quote:
... After blocking with Odyssey blocking buffer (LI-COR) or skim milk for 1 h ...
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No products found
because this supplier's products are not listed.
Quote:
... WDR5 blocking peptide (Bethyl) was used to examine WDR5 specificity in 1:1 and 1:2 (antibody ...
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No products found
because this supplier's products are not listed.
Quote:
... Peptides (GenScript, Inc.) were dissolved directly into the experimental buffer prior to each experiment ...
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No products found
because this supplier's products are not listed.
Gopinath Kulasekaran, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... Mouse monoclonal pan Arf (1D9) antibody was purchased from Abcam. Rabbit polyclonal Rab35 antibody was raised against GST-tagged full-length human Rab35 as previously described (Allaire et al. ...
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No products found
because this supplier's products are not listed.
Martina Damo, et al.,
bioRxiv - Immunology 2020
Quote:
... while P14 T cells were stained with antibodies specific for Thy1.1 (clone OX-7, BioLegend), CD8 (clone 53-6.7 ...
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No products found
because this supplier's products are not listed.
Quote:
... After protein blocking (DAKO) for 60 minutes ...
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No products found
because this supplier's products are not listed.
Quote:
... Biotin blocking reagent (Vector Laboratories) was applied to the tissue and slides were again incubated for 15 minutes at room temperature and then washed again in PBS ...
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No products found
because this supplier's products are not listed.
Manuel Rhiel, et al.,
bioRxiv - Biochemistry 2018
Quote:
... and the Arf isoforms were further purified via a run over a Superdex75 (16/60) column (GE Healthcare, USA) equilibrated in storage buffer (25 mM HEPES pH 7.4 [KOH] ...
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No products found
because this supplier's products are not listed.
Sebastian Sorge, et al.,
bioRxiv - Developmental Biology 2018
Quote:
... Blocking was done with 1% blocking reagent (PerkinElmer) in PBT (0.1% Tween-20) ...
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No products found
because this supplier's products are not listed.
Quote:
... or H3R2me2a peptide (Anaspec) were immobilized on 20μL Streptavidin sepharose resin (Thermo ...
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No products found
because this supplier's products are not listed.
Jun Imai, et al.,
bioRxiv - Immunology 2019
Quote:
... All peptides used in peptide loading experiments were obtained from Peptide Institute Inc ...
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No products found
because this supplier's products are not listed.
Quote:
... followed by blocking with 1% (w/v) casein blocking buffer (Bio-Rad, 161-0782). Membranes were incubated with streptavidin conjugated to horse radish peroxidase (1:7000 ...
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No products found
because this supplier's products are not listed.
Shunsuke Kataoka, et al.,
bioRxiv - Immunology 2019
Quote:
... CD8 T cells from spleens and lymph nodes of either P14 or FSF-Tim-3/E8i-Cre/P14 mice were purified by magnetic separation using a naïve CD8+ T cell isolation kit (Miltenyi Biotec, #130-104-075) and then mixed with gp33-pulsed B cells at a ratio of 1:1 and incubated for indicated times at 37°C.
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No products found
because this supplier's products are not listed.
Miranda P. Collier, et al.,
bioRxiv - Biophysics 2018
Quote:
Peptides were purchased from Biomatik at 99+% purity with acetylated N-termini when the N-terminal residue was Glu80 ...
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No products found
because this supplier's products are not listed.
Quote:
Peptides were synthesised by Fmoc-based solid-phase peptide synthesis on H-rink amide ChemMatrix® resin (Merck). The peptide sequence was assembled using an automated synthesiser (Syro II ...
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No products found
because this supplier's products are not listed.
Quote:
Peptides were identified using ProteinPilot 4.1 (SCIEX), searching the LudwigNR database (downloaded from http://apcf.edu.au as at 27 January 2012 ...
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No products found
because this supplier's products are not listed.
Quote:
... blocking anti-CXCR4 MAB21651 (247506, R&D Systems) or anti-CCR7 MAB3477 (4B12 ...
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No products found
because this supplier's products are not listed.
Eric T. Son, et al.,
bioRxiv - Immunology 2020
Quote:
... Blocking with mouse Fc Block (BD Biosciences, catalogue# 553141) was performed for 10 minutes at 4°C and either or both of mouse anti-PE (clone PE001 ...
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No products found
because this supplier's products are not listed.
Hasan Abbas, et al.,
bioRxiv - Pathology 2020
Quote:
... a mouse IgG blocking antibody (Jackson Immunoresearch) was used in the blocking buffer and an antigen retrieval step was performed by heating the slides in a pressure cooker in citrate buffer was also performed prior to incubation with the primary antibody ...
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No products found
because this supplier's products are not listed.
Quote:
... and blocking with TBS/Blocking buffer (Rockland), the membrane was probed with anti-DmNMNAT (1:8,000 ...
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No products found
because this supplier's products are not listed.
Erik Johnson, et al.,
bioRxiv - Systems Biology 2020
Quote:
... and an external reference peptide mix (Promega) was spiked into the sample at the concentration of 0.5 pmol/µl ...
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No products found
because this supplier's products are not listed.
Quote:
... Digestions with Peptide-N-Glycosidase F (PNGaseF, NEB) were carried out for 4h at 37°C in the presence of 1% NP40 and the buffer provided by the manufacturer (NEB) ...
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No products found
because this supplier's products are not listed.
Quote:
... agatoxin (Peptides International), and calmodulin (Sigma-Aldrich).
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No products found
because this supplier's products are not listed.
Quote:
... the blocking solution was replaced by primary antibodies diluted in blocking solution: α-Foxa2 1:1000 (Cell signaling #8186) and α-GFP 1:1000 (Aves Labs #1020) ...
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No products found
because this supplier's products are not listed.
Patricia E. Collins, et al.,
bioRxiv - Immunology 2018
Quote:
... For endogenous immunoprecipitation experiments TPL-2 was eluted using the TPL-2 blocking peptide by incubation at room temperature for 10 minutes with occasional agitation (Santa Cruz). Equal volumes of resuspended immunoprecipitates were analysed by western blot ...
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No products found
because this supplier's products are not listed.
Quote:
... The labeled peptides were further purified and separated from the unlabeled peptides by RP-HPLC using a Jupiter 300 Å C5 column (Phenomenex). The concentration of fluorescent peptides and the efficiency of labeling were determined by measuring the absorbance of the fluorescent dye and the peptides.
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No products found
because this supplier's products are not listed.
Quote:
... Peptides were dried and ACN was removed from the peptide containing sample by SpeedVac (Eppendorf, Germany) at 45°C ...
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No products found
because this supplier's products are not listed.
Quote:
... 3XFlag peptide was from APExBIO. Anti-Flag(M2 ...
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No products found
because this supplier's products are not listed.
He Gu, Bridget T. Hughes, Peter J. Espenshade,
bioRxiv - Cell Biology 2018
Quote:
... Eluting peptides were sprayed through 1 μm emitter tip (New Objective) at 2.0 kV directly into the Q-Exactive ...
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No products found
because this supplier's products are not listed.
Changwei Yu, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... The target library derived from the oocyte RNA polymerase II transcripts was PCR-amplified (15 cycles for P14 WT, 16 cycles for P14 Tbpl2−/− mutant) and purified using AMPure beads (Beckman Coulter) to remove short PCR artifacts (< 200bp ...
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No products found
because this supplier's products are not listed.
Quote:
... anti-Hcp (against synthetic peptide, Eurogentec #1510528 (35)) ...
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No products found
because this supplier's products are not listed.
J. Justin Milner, et al.,
bioRxiv - Immunology 2020
Quote:
... and small intestine epithelial Trm (CD103hiCD62Llo) P14 cells were sorted into TCL buffer (Qiagen) with 1% 2-Mercaptoethanol ...
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No products found
because this supplier's products are not listed.
Corey Tan, et al.,
bioRxiv - Immunology 2020
Quote:
... anti-CD40L blocking Ab (clone MR-1, BioXcell).
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No products found
because this supplier's products are not listed.
Quote:
... 100 µM TIRAP (TLR2 and TLR4) inhibitor peptide or control peptide (Novus Biologicals) were added to neutrophils at 1 h before incubation.
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No products found
because this supplier's products are not listed.
Jia Zhao, et al.,
bioRxiv - Developmental Biology 2017
Quote:
... or 20 VIVIT peptide (Tocris) was used to activate or inhibit NFAT ...
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No products found
because this supplier's products are not listed.
Shunsuke Kataoka, et al.,
bioRxiv - Immunology 2019
Quote:
... P14 TCR transgenic mice were obtained from Jackson Laboratories. E8i-Cre mice were obtained from Dario Vignali ...
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No products found
because this supplier's products are not listed.
Nadia Rajab, et al.,
bioRxiv - Systems Biology 2020
Quote:
... Blocking buffer (0.3M glycine buffer, 10% Goat Serum, 1XPBS) was added to ‘dry’ pellets on ice for 1 hour ...
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No products found
because this supplier's products are not listed.
Ravi Holani, et al.,
bioRxiv - Immunology 2018
Quote:
... ± synthethic LL-37 peptide (>98.6% purity, H-6224.0005; Bachem) (both at 1 μg/g) ...
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No products found
because this supplier's products are not listed.
Quote:
... Peptides were desalted with C18 UltraMicroSpin columns (The Nest Group) according to the manufacturer’s instructions and dried in a SpeedVac concentrator (Thermo Scientific) ...
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No products found
because this supplier's products are not listed.
Annie Lee, et al.,
bioRxiv - Neuroscience 2019
Quote:
... a peptide corresponding to the inverted sequence of Aβ42 (INV42; rPeptide and Creative Peptide) was processed as for Aβ42 oligomerization ...
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No products found
because this supplier's products are not listed.
Quote:
... Peptides were desalted via C18 Macro SpinColumns (Harvard Apparatus) and dried under vacuum centrifugation.
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No products found
because this supplier's products are not listed.
Quote:
Modified histone peptide arrays (Active Motif) were blocked with 5% dried milk in TBS-T (10 mM Tris-HCl pH 7.6 ...
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No products found
because this supplier's products are not listed.
Quote:
... rabbit anti-IR93a (peptide immunogen CGEFWYRRFRASRKRRQFTN, Proteintech, Rosemont ...
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No products found
because this supplier's products are not listed.
Omri M. Finkel, et al.,
bioRxiv - Microbiology 2019
Quote:
... 0.375 μl mixed rRNA gene-blocking peptide nucleic acids (PNAs; 1:1 mix of 100 μM plastid PNA and 100 μM mitochondrial PNA; PNA Bio), 0.5 μl Kapa dNTPs ...
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No products found
because this supplier's products are not listed.
Søs Skovsø, et al.,
bioRxiv - Physiology 2020
Quote:
... C-peptide (Alpco #80-CPTMS-E01), glucagon (Mercodia ...
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No products found
because this supplier's products are not listed.
Quote:
... OT-II peptide OVA323-339 (ISQAVHAAHAEINEAGR) was from InvivoGen (San Diego, CA) and kindly provided by Paula Oliver (Children’s Hospital of Philadelphia ...
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No products found
because this supplier's products are not listed.
Quote:
... Slides were blocked for 30 min with peptide blocking solution (Innovex Biosciences, Richmond, CA) and incubated with the following primary antibodies for 1 hr at RT ...