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Multi-color fluorescent Western blotting in one kit
Try multicolor fluorescent western blotting...
Cat# K-12021-010,
10 assays, USD $240.00/ea
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Valentina Palermo, et al.,
bioRxiv - Cell Biology 2018
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... the blots were detected using the Western blotting detection kit WesternBright ECL (Advansta) according to the manufacturer’s instructions ...
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... Western blot was performed using iBind Western Blot System (Life Technologies). The bovine WDR19 protein was detected using rabbit abx316410 anti-human-WDR19 antibody (diluted 1:2000 in iBind solution ...
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... All western blots were developed in ChemiDoc western blot apparatus from BioRad. For IHC ...
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... α-Tubulin (Sigma # T6199; western blot – 1:5000), MKP-3/DUSP6 (Abcam # ab76310 ...
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Antibodies for Western blot Western blot were as follows: IRE1α (3294 Cell signaling), Bip (3183 Cell signaling) ...
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Kyu-Hyeon Yeom, et al.,
bioRxiv - Molecular Biology 2018
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... Fluorescent band intensities of western blots and RNA gels were quantified with ImageQuant software (GE Healthcare).
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... Western blot was performed for AGO1 (abcam), α-Tubulin (Sigma) ...
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David Kuo, et al.,
bioRxiv - Immunology 2019
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Western blot analyses using a STAT4 (Santa Cruz) and HSP90 (Cell Signaling Technology ...
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Kasuen Kotagama, et al.,
bioRxiv - Molecular Biology 2019
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... For the western blot we used a primary anti-GFP antibody (Novus, NB600-303) (1:2000) and a fluorescent secondary antibody (LI-COR, 925-32211)(1:5000) ...
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Liangqi Xie, et al.,
bioRxiv - Molecular Biology 2019
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... Western blot was exposed to Western Lightning Plus-ECL (PerkinElmer) and imaged in a ChemiDoc MP (Bio-Rad ...
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... For western blot an anti-HA high affinity (Roche, cat: 11867423001) and a rat HRP were used ...
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... Anti-GFP (#632381) used for western blot was purchased from Clontech. Anti-GFP used for IP was purchased from Invitrogen (#G10362) ...
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... Reliablot® Western blot kit (Bethyl Inc.) was used for subsequent probing with rabbit antibodies.
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... Western blot analysis was performed using the Penta His HRP conjugate kit (Qiagen). To check for equal loading ...
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... Western blots were developed using Substrat HRP Immobilon Western (Merck Millipore, WBKLS0500).
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... Secondary polyclonal antibodies conjugated with HRP for western blot were from Dako and used at 1:5000 ...
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Maria Paz Zafra, et al.,
bioRxiv - Bioengineering 2018
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... Antibodies used for western blot analyses were: Cas9 (BioLegend, #844301), Actin (Abcam ...
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... biotinylated anti-LAP1 antibody for Western blot (WB) (BAF246; R&D Systems), polyclonal goat anti-LAP1 antibody (R&D Systems) ...
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Gang Zhang, Thomas Kruse, Jakob Nilsson,
bioRxiv - Cell Biology 2018
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... proteins were examined by western blot using following antibodies: Cyclin B1 (554177, 1:1000, BD Pharmingen ...
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... Western blots were developed with infrared-conjugated secondary antibodies (Rockland), detected on a Li-Cor Odyssey Imaging Station ...
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... Primary antibodies used for Western blot analysis were: p16 (10883-1-AP, Proteintech), p21 (2947S ...
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... Western blots were performed using NP antibody GTX125989 (GeneTex), Myc antibody GTX115046 (GeneTex) ...
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... Western blot analysis was conducted with either mouse anti-TcdA (Novus Biologicals) or mouse anti-FLAG (Sigma ...
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Martin P. Reichhardt, et al.,
bioRxiv - Immunology 2019
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... The blot was developed using ECL Western Blotting Substrate (Promega) and imaged using Amersham Hyperfilm ECL (GE).
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Dou Peng, et al.,
bioRxiv - Cell Biology 2019
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... Overexpression of C1orf109L-eGFP was checked by Western blot and fluorescence inverted microscope (Olympus IX71).
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... GAR 111-065-144) and peroxidase-conjugated streptavidin (016-030-084) for Western blot were from Jackson ImmunoResearch Laboratories ...
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... Larvae with positive fluorescent signals were collected under a fluorescent stereomicroscope (Leica M165FC). To ensure that this line was produced with a single chromosomal insertion ...
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... and identification by Western blot with an anti-MBP antibody (New England Biolabs (E-8038)) ...
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K.M. Elias, et al.,
bioRxiv - Cell Biology 2019
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... Using a fluorescent microscope (Nikon), the GFP signal was observed in transfected 293T cells ...
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... MCF 10A cells were cultured in the MEGM kit (Lonza) at 37°C with 5% CO2 ...
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... Membranes were washed in TBST solution three times for 5 min each and incubated in VECTASTAIN® ABC-AmP™ reagent (VECTASTAIN ABC-AmP Kit, for Rabbit IgG, Chemiluminescent Western Blot Detection, Vector Laboratories) for 10 minutes at room temperature ...
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... Western Blot cartridge in a SpectraMax i3 system (Molecular Devices, San Jose, CA, USA). Membranes were analyzed by ScanLater software.
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... and Western blot analysis using anti-His (A01620, Genscript) and anti-PSA-NCAM (MAB5324 ...
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... Eluates were tested in western blot analysis probing with anti-Khc (1:5000, Cytoskeleton), anti-Staufen (1:1000)(Krauss ...
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... mCherry fluorescent protein marker (Addgene), and ampicillin resistance gene ...
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... SIVkrc prevalence was assessed from serum for each individual with an HIV-2 western blot assay (MP Diagnostics HIV-2 Western Blot version 1.2; MP Biomedicals, Singapore) capable of detecting divergent SIVs (Lauck et al. ...
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Jiyon Lee, et al.,
bioRxiv - Cancer Biology 2018
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... the blots were analyzed using West-Zol and a western blot detection system (iNtRON Biotechnology, South Korea).
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Heather L. Drexler, et al.,
bioRxiv - Molecular Biology 2019
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... Western blots were performed using an antibody against Ser2-phosphorylated CTD (transcribing Pol II) (Active Motif, 3E10) or GAPDH (LifeTechnologies ...
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Cristina Gonzalez, et al.,
bioRxiv - Biophysics 2018
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... for nanobodies bands visualization Western Blot using anti-His-HRP antibody (clone GG11-8F.3.5.1, Miltenyi Biotec, Paris, France) at 1/5000 and Streptavidin HRP at 1/2000 (ThermoFischer Scientific ...
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... fluorescent beads of known concentrations (AccuCount Fluorescent Particles, 7.0-7.9 μm, Spherotech) were added to the cells ...
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Clover T-Y Su, et al.,
bioRxiv - Biophysics 2020
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Fluorescent particles of 1.001 μm diameter (Polysciences) were added to each sample to obtain 0.054% of final particle concentration by volume for all microrheology measurements ...
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Soraia Martins, et al.,
bioRxiv - Neuroscience 2019
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... and fluorescent images were obtained by a LSM 700 microscope (Carl Zeiss), and analyzed in Adobe Photoshop software (Adobe ...
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Toshimichi Yamada, et al.,
bioRxiv - Molecular Biology 2019
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... The fluorescent images were processed to nucleotide sequences using the analysis Pipeline supplied by Illumina.
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... Antibodies used on western blots were: mouse monoclonal [6G6] to RFP (Chromotek).
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Erin Fry, et al.,
bioRxiv - Genetics 2018
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... Fluorescent secondary Abs (Jackson Labs) were incubated overnight after a full day of washing in PBS-0.1 %Triton ...
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... kit or DNA Clean and Concentrator kit (Zymo, DCC-100) with distilled water elution to reduce volume and salt concentration from the reaction condition ...
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... Western blot was serviced by RayBiotech with the following antibodies ...
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... The following antibodies were used for western blot analysis: Cas9 (Diagenode C15310258, 1:1000) TCF4 (Abcam ab217668 ...
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... All fluorescent measurements were performed on a fluorescent plate reader (Synergy HT; BioTek Instruments, Winooski, VT) using a 480nm excitation and a 520 emission ...
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... TLC plates (silica gel with fluorescent indicator 254 nm, Fluka or Macherey-Nagel) were used for reaction monitoring ...