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No products found
because this supplier's products are not listed.
Zhuokun Li, et al.,
bioRxiv - Molecular Biology 2020
Quote:
Nuclease-free water (Ambion) was added to 6 ng ssCir DNA to achieve a 20-µl solution ...
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No products found
because this supplier's products are not listed.
Sara Soleman, et al.,
bioRxiv - Neuroscience 2018
Quote:
... and Nuclease-free water (Qiagen) were mixed to total volume 12 μl ...
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No products found
because this supplier's products are not listed.
Ayokunle O. Olanrewaju, et al.,
bioRxiv - Bioengineering 2019
Quote:
... was diluted in nuclease-free water (3098, Sigma) to lyse RBCs and reduce unwanted inhibition of RT activity by blood components such as immunoglobulins ...
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No products found
because this supplier's products are not listed.
Karolina Wołodko, et al.,
bioRxiv - Physiology 2020
Quote:
... and eluted into 20 μl of nuclease- free water (P1195; Promega). All libraries were prepared from 100 to 200 pg of cDNA using the Nextera XT Kit (Illumina ...
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No products found
because this supplier's products are not listed.
Quote:
... and eluted in 15 µl nuclease-free water (NEB). Purified and concentrated RNA was incubated at 70 °C for 2 min and immediately placed on ice to avoid secondary structure formation ...
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No products found
because this supplier's products are not listed.
Jason D Buenrostro, et al.,
bioRxiv - Genomics 2017
Quote:
... and 4.47 μl of nuclease-free water) (Illumina, FC-121-1030; Promega, G9441), here the transposition reactions were scaled down to compensate for cell loss during washes ...
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No products found
because this supplier's products are not listed.
Quote:
... DNA template (up to 8 μL) and nuclease-free water were loaded into a disposable droplet generator cartridge (Bio-Rad). After adding 70 μL of droplet generation oil (Bio-Rad) ...
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No products found
because this supplier's products are not listed.
Hung-Chang Chen, et al.,
bioRxiv - Immunology 2018
Quote:
... Cells frozen in lysis buffers were directly complemented with cold nuclease-free water plus RNAse inhibitor (2 U/μl; Clontech) up to 9.5 μl of total volume ...
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No products found
because this supplier's products are not listed.
Yanjing V. Liu, et al.,
bioRxiv - Genetics 2020
Quote:
... with addition of EDTA-free protease inhibitor (cOmplete tablet, freshly dissolved in nuclease free water to make a 100X stock, 5056489001, Roche) and lysed multiple times with resuspension every 2-3 mins during the 10mins incubation on ice ...
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No products found
because this supplier's products are not listed.
Colm Atkins, et al.,
bioRxiv - Immunology 2018
Quote:
... The cDNA was then diluted 1:3 in nuclease-free water (Corning). Relative gene expression (primers listed in Supplementary Table 1 ...
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No products found
because this supplier's products are not listed.
Quote:
... and 6.4 μl of nuclease-free water (VWR, Radnor, PA). The cycling parameters were ...
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No products found
because this supplier's products are not listed.
Elizabeth T. Abshire, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... RNA was eluted in 50 µL nuclease-free water and was analyzed using a Bioanalyzer Tapestation (Agilent), with RIN values between 9.6 and 10.0 ...
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No products found
because this supplier's products are not listed.
Kosuke Izumi, et al.,
bioRxiv - Genetics 2019
Quote:
... and then mounted with Entellan water-free mounting medium (Merck). The presence or absence of SCS was evaluated by manual inspection of 200 293FT cells and 150 HCT116 cells ...
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No products found
because this supplier's products are not listed.
Quote:
... water-immersion objective (UPSLAPO60XW, Olympus) by means of a f+350 mm lens (Thorlabs ...
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No products found
because this supplier's products are not listed.
Christopher Chukwudi Okonkwo, et al.,
bioRxiv - Bioengineering 2018
Quote:
... The reaction volume was made up with nuclease-free water (Amresco®, Solon, OH). The reaction mixture was incubated overnight at 16 °C ...
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No products found
because this supplier's products are not listed.
Elliot Zwelibanzi Jiyane, et al.,
bioRxiv - Microbiology 2019
Quote:
... each PCR assay was set-up with nuclease-free water as the negative control (Bioline, UK), and positive controls were not included due to financial constraints ...
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No products found
because this supplier's products are not listed.
Quote:
... and 7 mM Na2HPO4 in nuclease-free water) using either the Amaxa nucleofector system II (Lonza) under nucleofection program T-005 or the Amaxa 4D-nucleofector system (Lonza ...
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No products found
because this supplier's products are not listed.
Alfire Sidik, Groves B. Dixon, Johann K. Eberhart,
bioRxiv - Developmental Biology 2020
Quote:
... Samples were re-suspended with 50 μL of nuclease-free water and subsequently purified using the RNA Clean & Concentrator kit (Zymo, R1018). The concentration of each sample was determined using a Nanodrop spectrophotometer ...
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No products found
because this supplier's products are not listed.
Elektra K. Robinson, et al.,
bioRxiv - Immunology 2020
Quote:
One hundred μg aliquots of total RNA preparation were brought to 100 μl in nuclease free water and poly-A selected using NEXTflex Poly(A) Beads (BIOO Scientific Cat#NOVA-512980) according to the manufacturer’s instructions ...
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No products found
because this supplier's products are not listed.
Philip J Harrison, et al.,
bioRxiv - Cell Biology 2020
Quote:
... and HyClone RNase free water was obtained from GE Healthcare Cell Culture.
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No products found
because this supplier's products are not listed.
Takehiko Ogura, et al.,
bioRxiv - Plant Biology 2019
Quote:
... with a 60× water objective (Plan Apo VC 60×/1.2 WI water, Nikon) and Immersol WTM (Carl Zeiss) ...
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No products found
because this supplier's products are not listed.
Quote:
Five micrograms of small RNAs was dissolved in nuclease-free water and incubated with TEX (Lucigen) for 1 h at 30°C according to the manufacturer’s protocol ...
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No products found
because this supplier's products are not listed.
Quote:
... Total RNA was eluted in 30 μL nuclease-free water and stored at −80°C in low-bind tubes (Eppendorf) until further analysis.
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Prepared by a method developed at Worthington. Some degradation products may be present. ≥90%...
Cat# LS000290,
100 mg, USD $48.0/1ea
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Marlee K. Ng, et al.,
bioRxiv - Biochemistry 2019
Quote:
... Microccocal nuclease (MNase; Worthington) was added at a concentration of 10 units/1.0 × 107 cells then incubated at 37°C for 30 min ...
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No products found
because this supplier's products are not listed.
Quote:
... a 63x water (NA 1.2 water C Apo, Korr C Apochromat UV-VIS-IR, DIC) objective (Carl Zeiss), a long working distance 63x water (NA 1.15 water ...
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No products found
because this supplier's products are not listed.
Quote:
... Time-lapse movies were recorded using a 25× water-immersion objective (1.0 NA; Zeiss) and a 20× water-immersion objective (1.0 NA; Leica). Using an excitation wavelength of 900 nm ...
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No products found
because this supplier's products are not listed.
Shengjun Li, et al.,
bioRxiv - Plant Biology 2019
Quote:
... 6 μg genomic DNA in a total of 150 μl nuclease free water was sheared into ~8 kb with g-TUBEs (520079, Covaris) by following manufacturer’s instruction.
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No products found
because this supplier's products are not listed.
Quote:
... was diluted in Nuclease Free H2O (TekNova, Hollister, CA) to a total volume of 200 μL and total nucleic acids were extracted on the BioRobot EZ1 (QIAGEN ...
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No products found
because this supplier's products are not listed.
Quote:
... repair templates and nuclease-free water were added to the mixtures and loaded into pulled injection needles (1B100F-4, World Precision Instruments, Sarasota, FL). Individual injected P0 animals were transferred to new 6 cm NGM plates approximately 18 hours after injections ...
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No products found
because this supplier's products are not listed.
Baojin Ding, et al.,
bioRxiv - Cell Biology 2017
Quote:
... washed with water-free acetone and then followed by the stepwise embedding in epon (Electron Microscopy Sciences) mix with propylene oxide (EMS ...
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No products found
because this supplier's products are not listed.
Quote:
... under standard environmental conditions (12:12 hour light:dark cycle, 64 – 78C, 30-70% humidity, free access to reverse osmosis water, corn cob bedding and #2918 rodent chow, Envigo).
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No products found
because this supplier's products are not listed.
Quote:
... and TALE nucleases (Addgene plasmid #35432 and #35431).
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No products found
because this supplier's products are not listed.
Quote:
... and then treated with benzonase nuclease (Santa Cruz Biotechnology, TX) for 15 min at 37°C before the addition of an equal volume of 4X NuPAGE sample buffer ...
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No products found
because this supplier's products are not listed.
Quote:
... EDTA free (CalBiochem), 1 mM benzamadine (Sigma-Aldrich) ...
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No products found
because this supplier's products are not listed.
Quote:
... Y-27632 (Abcam, Ab120129, dissolved in water). Drugs were washed out by media replacement ...
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No products found
because this supplier's products are not listed.
Nazish Sayed, et al.,
bioRxiv - Immunology 2019
Quote:
... carrier-protein-free stocks from BD Biosciences ...
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No products found
because this supplier's products are not listed.
Quote:
... Samples were sonicated using the Bioruptor water bath sonicator (Diagenode) using the “high” setting with cycles of 30 sec “on” and 30 sec “off” ...
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No products found
because this supplier's products are not listed.
Quote:
... IFN-λ2 (Carrier Free, R&D Systems), 1000 IU/mL IL-4 (Carrier Free ...
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No products found
because this supplier's products are not listed.
David B. Chou, et al.,
bioRxiv - Bioengineering 2018
Quote:
... in meniscus-free 6 well plates (STEMCELL Technologies, 27370) as per manufacturer’s suggestions ...
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No products found
because this supplier's products are not listed.
C Bourges, et al.,
bioRxiv - Genetics 2020
Quote:
Oligonucleotide libraries were re-suspended in nuclease-free water and amplified by emulsion PCR (NEB Q5 polymerase, 30 cycles, Micellula DNA Emulsion & Purification Kit (Chimerx)) using primers containing SfiI restriction sites ...
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No products found
because this supplier's products are not listed.
Yonggyun Kim, et al.,
bioRxiv - Immunology 2019
Quote:
... total RNAs were resuspended in nuclease-free water and cDNAs were synthesized from ~1 μg of RNAs using Maxime RT Premix (Intron Biotechnology, Seoul, Korea) according to the manufacturer’s instruction.
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No products found
because this supplier's products are not listed.
Roberto Viviani, et al.,
bioRxiv - Pharmacology and Toxicology 2019
Quote:
... 1-10 ng template in 9 μl Nuclease-free water were added to 10.0 μL of 2× TaqMan® Universal PCR master mix (ABI/Life technologies, USA) and 1.0 μL of a 20× combined primers and probes mix (ABI/Life Technologies ...
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No products found
because this supplier's products are not listed.
Quote:
... RNA (obtained as 250 nmol syntheses of rU12 from IDT) was initially suspended in DMPC-treated RNase-free water (MP Biomedicals), supplemented with an equal volume of 2× SAXS buffer (300 mM KCl ...
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No products found
because this supplier's products are not listed.
Anatoly Urisman, et al.,
bioRxiv - Cancer Biology 2019
Quote:
SRM was performed on Waters nanoAcquity UPLC system (Waters) online with AB Sciex Qtrap 5500 mass spectrometer (AB Sciex). Digested peptide samples were loaded at 0.5 µg per injection and separated using two-buffer reverse phase chromatography (Buffer A = 0.1% formic acid in water ...
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No products found
because this supplier's products are not listed.
Quote:
... Preparatory high-performance liquid chromatography (HPLC) was performed on a Waters HPLC system (Waters 2535) using a C4 column (Phenomenex Jupiter C4 ...
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No products found
because this supplier's products are not listed.
Quote:
... fixed in 4% methanol-free formaldehyde (Polysciences) in phosphate buffer ...
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No products found
because this supplier's products are not listed.
Quote:
... or 63x water immersion objective (PerkinElmer, NA 1.15, WD 0.6 mm) and the system’s two sCMOS cameras (4.4-megapixel 2100×2100 ...
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No products found
because this supplier's products are not listed.
Quote:
... Water (40 μL) and Agencourt AMPure XP beads (Beckman Coulter; 72 μL) were added and incubated for 15 min at room temperature ...
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No products found
because this supplier's products are not listed.
Priya Chatterji, et al.,
bioRxiv - Physiology 2018
Quote:
... blocking was performed using Animal-Free Blocker (Vector Laboratories). For all staining ...
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No products found
because this supplier's products are not listed.
Rosemary C Challis, et al.,
bioRxiv - Neuroscience 2018
Quote:
NucleoBond Xtra Maxi Endotoxin-Free (EF) plasmid purification kit (Macherey-Nagel, cat. no. 740424.50) CRITICAL Triple transient transfection requires large amounts of capsid (22.8 μg/dish ...