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No products found
because this supplier's products are not listed.
Quote:
Nuclease-free water (Ambion) was added to 6 ng ssCir DNA to achieve a 20-µl solution ...
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No products found
because this supplier's products are not listed.
Sara Soleman, et al.,
bioRxiv - Neuroscience 2018
Quote:
... and Nuclease-free water (Qiagen) were mixed to total volume 12 μl ...
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No products found
because this supplier's products are not listed.
Ayokunle O. Olanrewaju, et al.,
bioRxiv - Bioengineering 2019
Quote:
... was diluted in nuclease-free water (3098, Sigma) to lyse RBCs and reduce unwanted inhibition of RT activity by blood components such as immunoglobulins ...
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No products found
because this supplier's products are not listed.
Karolina Wołodko, et al.,
bioRxiv - Physiology 2020
Quote:
... and eluted into 20 μl of nuclease- free water (P1195; Promega). All libraries were prepared from 100 to 200 pg of cDNA using the Nextera XT Kit (Illumina ...
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No products found
because this supplier's products are not listed.
Quote:
... and eluted in 15 µl nuclease-free water (NEB). Purified and concentrated RNA was incubated at 70 °C for 2 min and immediately placed on ice to avoid secondary structure formation ...
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No products found
because this supplier's products are not listed.
Jason D Buenrostro, et al.,
bioRxiv - Genomics 2017
Quote:
... and 4.47 μl of nuclease-free water) (Illumina, FC-121-1030; Promega, G9441), here the transposition reactions were scaled down to compensate for cell loss during washes ...
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No products found
because this supplier's products are not listed.
Quote:
... DNA template (up to 8 μL) and nuclease-free water were loaded into a disposable droplet generator cartridge (Bio-Rad). After adding 70 μL of droplet generation oil (Bio-Rad) ...
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No products found
because this supplier's products are not listed.
Hung-Chang Chen, et al.,
bioRxiv - Immunology 2018
Quote:
... Cells frozen in lysis buffers were directly complemented with cold nuclease-free water plus RNAse inhibitor (2 U/μl; Clontech) up to 9.5 μl of total volume ...
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No products found
because this supplier's products are not listed.
Yanjing V. Liu, et al.,
bioRxiv - Genetics 2020
Quote:
... with addition of EDTA-free protease inhibitor (cOmplete tablet, freshly dissolved in nuclease free water to make a 100X stock, 5056489001, Roche) and lysed multiple times with resuspension every 2-3 mins during the 10mins incubation on ice ...
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No products found
because this supplier's products are not listed.
Colm Atkins, et al.,
bioRxiv - Immunology 2018
Quote:
... The cDNA was then diluted 1:3 in nuclease-free water (Corning). Relative gene expression (primers listed in Supplementary Table 1 ...
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No products found
because this supplier's products are not listed.
Quote:
... and 6.4 μl of nuclease-free water (VWR, Radnor, PA). The cycling parameters were ...
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No products found
because this supplier's products are not listed.
Elizabeth T. Abshire, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... RNA was eluted in 50 µL nuclease-free water and was analyzed using a Bioanalyzer Tapestation (Agilent), with RIN values between 9.6 and 10.0 ...
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No products found
because this supplier's products are not listed.
Kosuke Izumi, et al.,
bioRxiv - Genetics 2019
Quote:
... and then mounted with Entellan water-free mounting medium (Merck). The presence or absence of SCS was evaluated by manual inspection of 200 293FT cells and 150 HCT116 cells ...
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No products found
because this supplier's products are not listed.
Quote:
... water-immersion objective (UPSLAPO60XW, Olympus) by means of a f+350 mm lens (Thorlabs ...
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No products found
because this supplier's products are not listed.
Christopher Chukwudi Okonkwo, et al.,
bioRxiv - Bioengineering 2018
Quote:
... The reaction volume was made up with nuclease-free water (Amresco®, Solon, OH). The reaction mixture was incubated overnight at 16 °C ...
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No products found
because this supplier's products are not listed.
Elliot Zwelibanzi Jiyane, et al.,
bioRxiv - Microbiology 2019
Quote:
... each PCR assay was set-up with nuclease-free water as the negative control (Bioline, UK), and positive controls were not included due to financial constraints ...
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No products found
because this supplier's products are not listed.
Quote:
... and 7 mM Na2HPO4 in nuclease-free water) using either the Amaxa nucleofector system II (Lonza) under nucleofection program T-005 or the Amaxa 4D-nucleofector system (Lonza ...
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No products found
because this supplier's products are not listed.
Alfire Sidik, Groves B. Dixon, Johann K. Eberhart,
bioRxiv - Developmental Biology 2020
Quote:
... Samples were re-suspended with 50 μL of nuclease-free water and subsequently purified using the RNA Clean & Concentrator kit (Zymo, R1018). The concentration of each sample was determined using a Nanodrop spectrophotometer ...
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No products found
because this supplier's products are not listed.
Elektra K. Robinson, et al.,
bioRxiv - Immunology 2020
Quote:
One hundred μg aliquots of total RNA preparation were brought to 100 μl in nuclease free water and poly-A selected using NEXTflex Poly(A) Beads (BIOO Scientific Cat#NOVA-512980) according to the manufacturer’s instructions ...
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No products found
because this supplier's products are not listed.
Philip J Harrison, et al.,
bioRxiv - Cell Biology 2020
Quote:
... and HyClone RNase free water was obtained from GE Healthcare Cell Culture.
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No products found
because this supplier's products are not listed.
Takehiko Ogura, et al.,
bioRxiv - Plant Biology 2019
Quote:
... with a 60× water objective (Plan Apo VC 60×/1.2 WI water, Nikon) and Immersol WTM (Carl Zeiss) ...
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No products found
because this supplier's products are not listed.
Quote:
Five micrograms of small RNAs was dissolved in nuclease-free water and incubated with TEX (Lucigen) for 1 h at 30°C according to the manufacturer’s protocol ...
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No products found
because this supplier's products are not listed.
Quote:
... Total RNA was eluted in 30 μL nuclease-free water and stored at −80°C in low-bind tubes (Eppendorf) until further analysis.
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Prepared by a method developed at Worthington. Some degradation products may be present. ≥90%...
Cat# LS000290,
100 mg, USD $48.0/1ea
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Marlee K. Ng, et al.,
bioRxiv - Biochemistry 2019
Quote:
... Microccocal nuclease (MNase; Worthington) was added at a concentration of 10 units/1.0 × 107 cells then incubated at 37°C for 30 min ...
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No products found
because this supplier's products are not listed.
Quote:
... a 63x water (NA 1.2 water C Apo, Korr C Apochromat UV-VIS-IR, DIC) objective (Carl Zeiss), a long working distance 63x water (NA 1.15 water ...
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No products found
because this supplier's products are not listed.
Quote:
... Time-lapse movies were recorded using a 25× water-immersion objective (1.0 NA; Zeiss) and a 20× water-immersion objective (1.0 NA; Leica). Using an excitation wavelength of 900 nm ...
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No products found
because this supplier's products are not listed.
Shengjun Li, et al.,
bioRxiv - Plant Biology 2019
Quote:
... 6 μg genomic DNA in a total of 150 μl nuclease free water was sheared into ~8 kb with g-TUBEs (520079, Covaris) by following manufacturer’s instruction.
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No products found
because this supplier's products are not listed.
Quote:
... was diluted in Nuclease Free H2O (TekNova, Hollister, CA) to a total volume of 200 μL and total nucleic acids were extracted on the BioRobot EZ1 (QIAGEN ...
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No products found
because this supplier's products are not listed.
Quote:
... repair templates and nuclease-free water were added to the mixtures and loaded into pulled injection needles (1B100F-4, World Precision Instruments, Sarasota, FL). Individual injected P0 animals were transferred to new 6 cm NGM plates approximately 18 hours after injections ...
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No products found
because this supplier's products are not listed.
Kwok Kin Lee, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... The RNA pellet was dissolved in RNase-free water (Santa Cruz, Cat. No. sc-204391).
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No products found
because this supplier's products are not listed.
Baojin Ding, et al.,
bioRxiv - Cell Biology 2017
Quote:
... washed with water-free acetone and then followed by the stepwise embedding in epon (Electron Microscopy Sciences) mix with propylene oxide (EMS ...
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No products found
because this supplier's products are not listed.
Quote:
... under standard environmental conditions (12:12 hour light:dark cycle, 64 – 78C, 30-70% humidity, free access to reverse osmosis water, corn cob bedding and #2918 rodent chow, Envigo).
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No products found
because this supplier's products are not listed.
Quote:
... and TALE nucleases (Addgene plasmid #35432 and #35431).
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No products found
because this supplier's products are not listed.
Quote:
... EDTA free (CalBiochem), 1 mM benzamadine (Sigma-Aldrich) ...
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No products found
because this supplier's products are not listed.
Quote:
... Y-27632 (Abcam, Ab120129, dissolved in water). Drugs were washed out by media replacement ...
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No products found
because this supplier's products are not listed.
Domokos I. Lauko, et al.,
bioRxiv - Cell Biology 2020
Quote:
... using a 40x water immersion objective (PerkinElmer). Images were analyzed on Harmony image analysis software (PerkinElmer ...
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No products found
because this supplier's products are not listed.
Nazish Sayed, et al.,
bioRxiv - Immunology 2019
Quote:
... carrier-protein-free stocks from BD Biosciences ...
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No products found
because this supplier's products are not listed.
Quote:
... Samples were sonicated using the Bioruptor water bath sonicator (Diagenode) using the “high” setting with cycles of 30 sec “on” and 30 sec “off” ...
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No products found
because this supplier's products are not listed.
Quote:
... IFN-λ2 (Carrier Free, R&D Systems), 1000 IU/mL IL-4 (Carrier Free ...
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No products found
because this supplier's products are not listed.
David B. Chou, et al.,
bioRxiv - Bioengineering 2018
Quote:
... in meniscus-free 6 well plates (STEMCELL Technologies, 27370) as per manufacturer’s suggestions ...
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No products found
because this supplier's products are not listed.
C Bourges, et al.,
bioRxiv - Genetics 2020
Quote:
Oligonucleotide libraries were re-suspended in nuclease-free water and amplified by emulsion PCR (NEB Q5 polymerase, 30 cycles, Micellula DNA Emulsion & Purification Kit (Chimerx)) using primers containing SfiI restriction sites ...
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No products found
because this supplier's products are not listed.
Yonggyun Kim, et al.,
bioRxiv - Immunology 2019
Quote:
... total RNAs were resuspended in nuclease-free water and cDNAs were synthesized from ~1 μg of RNAs using Maxime RT Premix (Intron Biotechnology, Seoul, Korea) according to the manufacturer’s instruction.
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No products found
because this supplier's products are not listed.
Quote:
... 1-10 ng template in 9 μl Nuclease-free water were added to 10.0 μL of 2× TaqMan® Universal PCR master mix (ABI/Life technologies, USA) and 1.0 μL of a 20× combined primers and probes mix (ABI/Life Technologies ...
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No products found
because this supplier's products are not listed.
Magda Lewandowska, et al.,
bioRxiv - Evolutionary Biology 2020
Quote:
... both suspended in sterile RNase-free endotoxin-free water to a final concentration of 90 ng/μl (Invivogen). Each experiment was performed in triplicates and each biological replicate was composed of 100-150 injected zygotes per time point ...
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No products found
because this supplier's products are not listed.
Quote:
... RNA (obtained as 250 nmol syntheses of rU12 from IDT) was initially suspended in DMPC-treated RNase-free water (MP Biomedicals), supplemented with an equal volume of 2× SAXS buffer (300 mM KCl ...
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No products found
because this supplier's products are not listed.
Quote:
... Preparatory high-performance liquid chromatography (HPLC) was performed on a Waters HPLC system (Waters 2535) using a C4 column (Phenomenex Jupiter C4 ...
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No products found
because this supplier's products are not listed.
Quote:
... fixed in 4% methanol-free formaldehyde (Polysciences) in phosphate buffer ...
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No products found
because this supplier's products are not listed.
Quote:
... Water (40 μL) and Agencourt AMPure XP beads (Beckman Coulter; 72 μL) were added and incubated for 15 min at room temperature ...
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No products found
because this supplier's products are not listed.
Priya Chatterji, et al.,
bioRxiv - Physiology 2018
Quote:
... blocking was performed using Animal-Free Blocker (Vector Laboratories). For all staining ...
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No products found
because this supplier's products are not listed.
Rosemary C Challis, et al.,
bioRxiv - Neuroscience 2018
Quote:
NucleoBond Xtra Maxi Endotoxin-Free (EF) plasmid purification kit (Macherey-Nagel, cat. no. 740424.50) CRITICAL Triple transient transfection requires large amounts of capsid (22.8 μg/dish ...