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No products found
because this supplier's products are not listed.
Katie F. Loveson, Helen L. Fillmore,
bioRxiv - Cancer Biology 2020
Quote:
... human PDGF-AA (10 ng/mL) and PDGF-BB (10 ng/mL) (Peprotech) and heparin (2 ng/mL ...
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No products found
because this supplier's products are not listed.
Sara Cleto, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... cell-free spent medium (270 mL) was supplemented with an internal control (0.5 mM Tyr-Tyr-Tyr eluent concentration, Sigma-Aldrich T2007). The spent medium was subsequently loaded into a Sep-Pak tC18 column (100 mg) ...
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No products found
because this supplier's products are not listed.
Quote:
... P-Tyr (Cell Signaling 8954S) (1:2,000) ...
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No products found
because this supplier's products are not listed.
Jelena Ostojić, et al.,
bioRxiv - Cell Biology 2020
Quote:
... rabbit anti-TYR (ab61284, Abcam), rabbit anti-DCT (ab74073 ...
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No products found
because this supplier's products are not listed.
Quote:
... or PDGF-BB (50 ng/ml; ThermoFisher Scientific) for 10 min ...
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No products found
because this supplier's products are not listed.
Quote:
... PDGF (220-BB, R&D Systems) and Endothelin 1 (1160/100U ...
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No products found
because this supplier's products are not listed.
Rana N. Ozdeslik, et al.,
bioRxiv - Physiology 2019
Quote:
... anti-TYR (Santa Cruz Biotechnology, sc-7833), anti-β-actin (ThermoFisher Scientific ...
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No products found
because this supplier's products are not listed.
Quote:
... Human SMAD4 double mutant (Tyr 353 Phe and Tyr 412 Phe) was obtained by using PCR and Gibson Assembly® Cloning Kit (NEB). All plasmid constructs were confirmed by sequencing ...
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No products found
because this supplier's products are not listed.
Ioanna Tzani, et al.,
bioRxiv - Cell Biology 2020
Quote:
... the light chain by the anti-human kappa light chain antibody (1:2,500; Biorad, cat.no. STAR 127) and GAPDH by the anti-GAPDH monoclonal antibody (1:10,000 ...
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No products found
because this supplier's products are not listed.
Quote:
C57BL/6J-Tyr mice were purchased from Jackson Laboratories and C57BL/6NCrl mice were purchased from Charles River Laboratories at 8 weeks of age and allowed to acclimate for 9 days at UTHSC before tissue collection ...
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No products found
because this supplier's products are not listed.
Quote:
... mouse anti clathrin heavy chain (BD Biosciences), mouse anti-STIM1 (Cell Signalling) ...
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No products found
because this supplier's products are not listed.
Quote:
... All polymerase chain reactions (PCRs) were performed using Taq DNA polymerase (Roche). PCRs for construction of recombinant pGKL plasmids were performed using mixture of Taq DNA polymerase (Roche ...
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No products found
because this supplier's products are not listed.
William B. Black, et al.,
bioRxiv - Synthetic Biology 2020
Quote:
... Polymerase chain reaction (PCR) fragments were generated using PrimeSTAR Max DNA Polymerase (TaKaRa). The method for plasmid construction is described below.
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No products found
because this supplier's products are not listed.
Quote:
... and polyclonal rabbit anti p-FAK (Tyr 397) (Merck Millipore). Secondary antibodies used were anti-mouse (1:15000 ...
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No products found
because this supplier's products are not listed.
Cavan P. Bailey, et al.,
bioRxiv - Cancer Biology 2019
Quote:
... 20ng/mL EGF/bFGF and 10ng/mL PDGF-AA/PDGF-BB (all from Shenandoah Biotechnology). DIPG IV possess a H3.1-K27M mutation ...
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No products found
because this supplier's products are not listed.
Quote:
... Polymerase chain reactions were performed using 2 U Gotaq (Promega), 1X Gotaq buffer (Promega) ...
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No products found
because this supplier's products are not listed.
G. Sugiyarto, et al.,
bioRxiv - Immunology 2018
Quote:
... α-TCR β-chain (H57-597; Biolegend) and 5 μg of PE-labelled GSW11- specific tetramers ...
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No products found
because this supplier's products are not listed.
Quote:
... IgM (μ chain specific) (Jackson ImmunoResearch). Data were analyzed using FlowJo software ...
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No products found
because this supplier's products are not listed.
Quote:
The genes encoding the variable heavy chain and variable light chain of Ab10 and MAb4-5[28] were synthesized (Integrated DNA Technologies, GenScript) and fused with human heavy chain constant region gene (IgG1 ...
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No products found
because this supplier's products are not listed.
Mohammad G Mohammad, et al.,
bioRxiv - Physiology 2020
Quote:
... The polymerase chain reaction (PCR) were carried out using Rotor Gene Q (Qiagen, Hilden, Germany) with initial incubation at 95 °C for 15 min ...
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No products found
because this supplier's products are not listed.
Nathan W. Zammit, et al.,
bioRxiv - Immunology 2019
Quote:
... coli was added to 2 µg of K48-ubiquitin chains of mixed chain length (Ub2-Ub7) or to purified tetra-ubiquitin (Ub4) (#UC-230, UC-210, Boston Biochem). A20 OTU domains added to mixed-length K48-ubiquitin chains were incubated in 100 µl of deubiquitin buffer (50 mM HEPES pH 8.0 ...
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No products found
because this supplier's products are not listed.
Quote:
... PolyP chains used for this analysis are an average of 75 residues in length and were purchased from Kerafast (Medium chain, EUI005).
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No products found
because this supplier's products are not listed.
Quote:
... MA) real time quantitative polymerase chain reaction (RT-qPCR) analysis in an Eco system (Illumina, San Diego). RT-qPCR threshold cycle (CT ...
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No products found
because this supplier's products are not listed.
Quote:
... 50 using RFP-Trap single chain antibodies (Chromotek). Enrichment of specific DNA fragments was validated by qPCR at the ARR7 promoter region24 ...
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No products found
because this supplier's products are not listed.
Marcel Lagedroste, et al.,
bioRxiv - Biochemistry 2019
Quote:
... a polymerase chain reaction using PfuUltra II Fusion DNA polymerase (Agilent Technologies), the template pIL-SVnisBTC and the appropriate pair of oligonucleotides (Supplementary File 4 ...
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No products found
because this supplier's products are not listed.
Quote:
... human PDGF-AA (10 ng/mL) and PDGF-BB (10 ng/mL) (Shenandoah) and heparin (2 ng/mL) (Stem Cell Technologies).
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No products found
because this supplier's products are not listed.
Peng Du, et al.,
bioRxiv - Pharmacology and Toxicology 2018
Quote:
... TGFβ and PDGF ELISA kits were purchased from Elabscience Biotechnology Co. ...
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No products found
because this supplier's products are not listed.
Quote:
... anti-EphA4-Tyr(602) (1:50, ECM Biosciences, EP2731); anti-EphB6 (1:50 ...
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No products found
because this supplier's products are not listed.
Quote:
... Polymerase chain reaction (PCR) was performed with a Master Cycler Gradient (Eppendorf) using Taq DNA Polymerase (Q BIOgene ...
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No products found
because this supplier's products are not listed.
Quote:
... 10μL polymerase chain reactions (PCR) contained 0.35 units AmpliTaq® DNA polymerase (ABI; Applied Biosystems™ ...
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No products found
because this supplier's products are not listed.
Quote:
... and light chain of neurofilament (NF-L) (MyBioSource) were used to measure the levels of these proteins in the serum samples from the SCI rats according to the supplier ...
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No products found
because this supplier's products are not listed.
Samantha L Scudder, et al.,
bioRxiv - Neuroscience 2019
Quote:
... N-succinyl-Leu-Leu-Val-Tyr-7-amino-4-methylcoumarin (Suc-LLVY-AMC) substrate was used (BACHEM). GST-Ubl and His-UIM constructs were a gift from the Alfred Goldberg lab ...
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No products found
because this supplier's products are not listed.
Quote:
... Light chain standards ranging from 3 – 300 ng/mL were prepared from purified human Bence Jones λ light chain (Bethyl Laboratories, P80-127). Plates were incubated at 37 °C for 1.5 h with shaking ...
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No products found
because this supplier's products are not listed.
Quote:
... of human light chain and PG9 heavy chain expression plasmids were sequenced using Sanger sequencing (Eton Biosciences). 25µg each of filter sterile PG9 heavy chain (IgG ...
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No products found
because this supplier's products are not listed.
Shivani Upadhyaya, et al.,
bioRxiv - Plant Biology 2018
Quote:
... anti-Tubulin alpha chain (Agrisera, AS10 680), anti-D1 protein of PSII (Agrisera ...
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No products found
because this supplier's products are not listed.
Quote:
... chain-terminating 3'-O-methyl-NTPs (RiboMed; 1.25 μl 400 μM 3'-O-methyl-UTP ...
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No products found
because this supplier's products are not listed.
Quote:
... anti-polyglutamate chain (1:500 dilution, IN105, AdipoGen), anti-Δ2 tubulin (1:500 dilution ...
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No products found
because this supplier's products are not listed.
Po-Kuan Chao, et al.,
bioRxiv - Neuroscience 2017
Quote:
... mouse monoclonal anti-clathrin heavy chain antibody (1:1000; Cat. GTX22731; GeneTex), or mouse monoclonal anti-β-actin antibody (1:5000 ...
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No products found
because this supplier's products are not listed.
Quote:
... Supernatant was run through a column of CaptoL resin to capture the light chains of antibodies (GE Life Sciences). Antibodies were dialyzed against phosphate buffered saline before moving into size exclusion chromatography to separate out the desired dimeric or secretory antibodies using a HiLoad 26/600 Superdex 200 pg size exclusion column (GE Healthcare Life Sciences) ...
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No products found
because this supplier's products are not listed.
Quote:
... heavy and light chain DNA sequences were purchased from Twist Biosciences and cloned separately into the pHLSec mammalian expression vector (Addgene, #99845) using AgeI and XhoI restriction sites ...
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No products found
because this supplier's products are not listed.
Lauren E. Neidig, et al.,
bioRxiv - Cell Biology 2018
Quote:
... Myosin light chain 2 (Proteintech, 10906-1-AP) and tdTomato (Abcam ...
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No products found
because this supplier's products are not listed.
Quote:
The P12Y oligonucleotide linked to tyrosine at the 3’-end (5’-HO-GAAAAAAGAGTT-PO4-Tyr-3’, TopoGEN) was labeled at the 5’-end with 32P ...
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No products found
because this supplier's products are not listed.
Andrés F. Londoño, et al.,
bioRxiv - Pathology 2018
Quote:
... mu chain specific (1:500 dilution, Vector Laboratories, Burlingame, CA), was added to the wells and incubated for 30 minutes in a humidified chamber ...
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No products found
because this supplier's products are not listed.
Tsuyoshi Waku, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... and mixed with 2 mM ATP and 0.1 mM fluorogenic peptide substrate Suc-Leu-Leu-Val-Tyr-AMC (Peptide Institute). Fluorescence (380 nm excitation ...
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No products found
because this supplier's products are not listed.
Quote:
... or rabbit anti-human laminin α3 chain antibody (1:1000 dilution, Assay biotech). After incubation with biotinylated anti-rabbit antibody for 60 min at room temperature (RT) ...
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No products found
because this supplier's products are not listed.
Quote:
... we detected bound Abs by incubating plates with 50 μl HRP-conjugated rat anti-mouse IgG kappa chain (Southern Biotech) for 1 h at room temperature ...
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No products found
because this supplier's products are not listed.
Daisy X. Ji, et al.,
bioRxiv - Immunology 2018
Quote:
... Mouse anti-mouse IFNAR1 (MAR1-5A3) and isotype control (GIR208, mouse anti-human IFNGR-α chain) were purchased from Leinco Technologies Inc ...
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No products found
because this supplier's products are not listed.
Quote:
... transcription was chased by adding 40 µM NTPs mix together with 50 µM of each type of 3’-deoxynucleotide RNA chain terminators (3’dATP, 3’dCTP, 3’dGTP, 3’dUTP, TriLink Biotechnologies). The reactions were allowed to proceed at room temperature for 10 min before terminated by adding the 2× urea stop buffer ...
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No products found
because this supplier's products are not listed.
Mohammad-Reza Ghovanloo, et al.,
bioRxiv - Biophysics 2020
Quote:
1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC-d31, sn-1 chain perdeuterated) was obtained from Avanti Polar Lipids (Alabaster, AL). The POPC-d31:CBD sample was prepared with ∼50 mg lipid and 3.4 mg of CBD for a ratio of POPC/CBD 8:2 ...
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No products found
because this supplier's products are not listed.
Quote:
... Odorants were diluted in caprylic/capric medium chain triglycerides (C3465, Spectrum Chemical Mfg. Corp.). Characterization of olfactometer performance was completed using a photo-ionization detector (PID ...