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No products found
because this supplier's products are not listed.
Prakriti Sharma Ghimire, et al.,
bioRxiv - Bioengineering 2020
Quote:
... Peptides were then produced by trypsin digestion (50 ng trypsin, Promega Sequence Grade, Modified) (Liu et al ...
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No products found
because this supplier's products are not listed.
Quote:
... a blocking FLAG peptide (Sigma), used at a 100 μg/ml concentration ...
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No products found
because this supplier's products are not listed.
Quote:
... After blocking trypsin activity with soybean trypsin inhibitor (# R007100, ThermoFisher Scientific), cells were grown in KBM-2 medium (# CC-3107 ...
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No products found
because this supplier's products are not listed.
Quote:
... After blocking in 1% blocking buffer (11096176001, Roche) for 1 h ...
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No products found
because this supplier's products are not listed.
Tanne van der Wal, et al.,
bioRxiv - Cell Biology 2019
Quote:
... Membranes were blocked in 1:1 TBS:Odyssey Blocking buffer (LI-COR) and incubated overnight at 4°C with primary antibodies (rabbit anti-TMEM98 ...
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No products found
because this supplier's products are not listed.
Elinor Gottschalk, et al.,
bioRxiv - Immunology 2019
Quote:
... 1 μM of hgp100 peptide (GenScript) in complete DMEM was added for 1 hour at 37°C before co-culture with the pmel-1 T cells ...
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No products found
because this supplier's products are not listed.
Quote:
... WDR5 blocking peptide (Bethyl) was used to examine WDR5 specificity in 1:1 and 1:2 (antibody ...
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No products found
because this supplier's products are not listed.
Ilja Vietor, et al.,
bioRxiv - Cell Biology 2019
Quote:
... Specificity of the antibody was tested incubating part of the membrane with the blocking peptide (1 μg/mL; Abcam, ab66772). Chemiluminescence reagents (Supersignal West Pico Chemiluminescence substrate ...
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No products found
because this supplier's products are not listed.
Sebastian Sorge, et al.,
bioRxiv - Developmental Biology 2018
Quote:
... Blocking was done with 1% blocking reagent (PerkinElmer) in PBT (0.1% Tween-20) ...
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No products found
because this supplier's products are not listed.
Quote:
... followed by blocking with 1% (w/v) casein blocking buffer (Bio-Rad, 161-0782). Membranes were incubated with streptavidin conjugated to horse radish peroxidase (1:7000 ...
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No products found
because this supplier's products are not listed.
Quote:
... or PD-1 blocking Ab (clone EH12.2H7, BioLegend) were added to the media and incubated at 37 °C for 72 hrs ...
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No products found
because this supplier's products are not listed.
Quote:
... the blocking solution was replaced by primary antibodies diluted in blocking solution: α-Foxa2 1:1000 (Cell signaling #8186) and α-GFP 1:1000 (Aves Labs #1020) ...
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No products found
because this supplier's products are not listed.
Anatoly Urisman, et al.,
bioRxiv - Cancer Biology 2019
Quote:
... 4 mg of trypsin digested peptides were separately processed on an AKTA Purifier UPLC system (GE Healthcare) fitted with an in-house column packed with 5-μm TiO2 beads (GL Sciences ...
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No products found
because this supplier's products are not listed.
Quote:
... permeabilized cells were blocked with blocking buffer (1% gelatin (Merck), 1 % BSA (Merck ...
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No products found
because this supplier's products are not listed.
Daniela Pankova, et al.,
bioRxiv - Cancer Biology 2018
Quote:
... After protein blocking (DAKO) for 60 minutes ...
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No products found
because this supplier's products are not listed.
Yitzhak Reizel, et al.,
bioRxiv - Genetics 2020
Quote:
... Biotin blocking reagent (Vector Laboratories) was applied to the tissue and slides were again incubated for 15 minutes at room temperature and then washed again in PBS ...
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Supplied as dialyzed and lyophilized powder.
Cat# LS003704,
10 gm, USD $970.0/1ea
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Quote:
... All viral growth was carried out in MEM with 1 µg/mL trypsin treated with L-(tosylamido-2-phenyl) ethyl chloromethyl ketone (TPCK-treated trypsin; Worthington), 1mM HEPES ...
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No products found
because this supplier's products are not listed.
Quote:
... 50 μL of 1 uM biotinylated peptides (Anaspec) in 0.2% PBST were incubated at room temperature for 1 hour at 800 RPM ...
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No products found
because this supplier's products are not listed.
Patricia E. Collins, et al.,
bioRxiv - Immunology 2018
Quote:
... For endogenous immunoprecipitation experiments TPL-2 was eluted using the TPL-2 blocking peptide by incubation at room temperature for 10 minutes with occasional agitation (Santa Cruz). Equal volumes of resuspended immunoprecipitates were analysed by western blot ...
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No products found
because this supplier's products are not listed.
Luca Cassetta, et al.,
bioRxiv - Immunology 2020
Quote:
... or 1% (v/v) CD16/CD32 Fc blocking reagent (BD Pharmingen; for mouse cells) for 1 hour on ice to block Fc receptors ...
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No products found
because this supplier's products are not listed.
Quote:
... rabbit serum at 1:200 in blocking buffer and Alexa Fluor 488 anti-rabbit (Jackson ImmunoResearch) at 1:200 ...
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No products found
because this supplier's products are not listed.
Quote:
... and FcR blocking reagent (Miltenyi Biotec, dilution 1:5) or isotype control antibody (mouse IgG1 ...
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No products found
because this supplier's products are not listed.
Jun Imai, et al.,
bioRxiv - Immunology 2019
Quote:
... All peptides used in peptide loading experiments were obtained from Peptide Institute Inc ...
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No products found
because this supplier's products are not listed.
Quote:
... and trypsin were acquired from Corning. Fetal Bovine Serum (FBS ...
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No products found
because this supplier's products are not listed.
Joshua Johnson, et al.,
bioRxiv - Physiology 2020
Quote:
... PD-1 blocking antibody (R&D Systems/Techne, #AF1086) was included at the 10 µg/ml ...
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No products found
because this supplier's products are not listed.
Miranda P. Collier, et al.,
bioRxiv - Biophysics 2018
Quote:
Peptides were purchased from Biomatik at 99+% purity with acetylated N-termini when the N-terminal residue was Glu80 ...
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No products found
because this supplier's products are not listed.
Quote:
... and blocking with TBS/Blocking buffer (Rockland), the membrane was probed with anti-DmNMNAT (1:8,000 ...
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No products found
because this supplier's products are not listed.
Yao Yao, et al.,
bioRxiv - Biophysics 2020
Quote:
... Trypsin-ultra™ (NEB Inc.) was added to all samples at final ratio of 1:50 by mass and the samples were digested for 30 min at 37 °C ...
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No products found
because this supplier's products are not listed.
Quote:
Peptides were identified using ProteinPilot 4.1 (SCIEX), searching the LudwigNR database (downloaded from http://apcf.edu.au as at 27 January 2012 ...
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No products found
because this supplier's products are not listed.
Weishan Huang, Junjie Luo, Avery August,
bioRxiv - Immunology 2018
Quote:
... or 1 μg/ml OVA257-264 peptide (Peptides International, Louisville, KY), in the presence of 5 - 10 μg/ml Brefeldin A (BFA ...
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No products found
because this supplier's products are not listed.
Quote:
... or mouse anti-2A peptide antibody (1:500, Novus Biologicals, NBP2-59627, LOT# A-1). After washing three times with 0.1 % Triton X-100 in PBS ...
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No products found
because this supplier's products are not listed.
Quote:
... Peptides were dried and ACN was removed from the peptide containing sample by SpeedVac (Eppendorf, Germany) at 45°C ...
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No products found
because this supplier's products are not listed.
Corey Tan, et al.,
bioRxiv - Immunology 2020
Quote:
... anti-CD40L blocking Ab (clone MR-1, BioXcell).
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No products found
because this supplier's products are not listed.
Quote:
... guinea pig anti-IR25a (peptide immunogen SKAALRPRFNQYPATFKPRF, Proteintech, Rosemont, IL, USA, 1:200), mouse anti-GFP (1:200 ...
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No products found
because this supplier's products are not listed.
Ravi Holani, et al.,
bioRxiv - Immunology 2018
Quote:
... formyl peptide receptor-like 1(FPRL1; WRW4, 2262; Tocris Bioscience), matrix metalloproteinases (MMP ...
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No products found
because this supplier's products are not listed.
He Gu, Bridget T. Hughes, Peter J. Espenshade,
bioRxiv - Cell Biology 2018
Quote:
... Eluting peptides were sprayed through 1 μm emitter tip (New Objective) at 2.0 kV directly into the Q-Exactive ...
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No products found
because this supplier's products are not listed.
Anastasia Thoma, et al.,
bioRxiv - Physiology 2020
Quote:
... and sub-cultured using 0.05% Trypsin/0.53 mM EDTA (1×) (Lonza, Nottingham, UK). Myoblasts were treated with the pharmaceutical ER stress inducer tunicamycin (0.1 μg/ml ...
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No products found
because this supplier's products are not listed.
Quote:
... The labeled peptides were further purified and separated from the unlabeled peptides by RP-HPLC using a Jupiter 300 Å C5 column (Phenomenex). The concentration of fluorescent peptides and the efficiency of labeling were determined by measuring the absorbance of the fluorescent dye and the peptides.
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No products found
because this supplier's products are not listed.
Shan Huang, et al.,
bioRxiv - Pathology 2019
Quote:
... After blocking with 1% bovine serum albumin (BSA) (VWR, Radnor, PA, US), the membranes were probed with 1 μg/mL mAb 9G3 against the glycosylated epitope ‘KEPAPTTT’ in the lubricin mucin domain (Merck KGaA ...
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No products found
because this supplier's products are not listed.
Quote:
... 3XFlag peptide was from APExBIO. Anti-Flag(M2 ...
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No products found
because this supplier's products are not listed.
Quote:
... Then the cells were incubated with blocking buffer (1XPBS, 1% BSA, 0.3M glycine, 0.1% Tween 20) supplemented with 5% goat serum for 1 h at room temperature ...
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No products found
because this supplier's products are not listed.
Carola Städele, Wolfgang Stein,
bioRxiv - Neuroscience 2015
Quote:
... FMRF-like peptide F1 (TNRNFLRF-NH2, Bachem) and cimetidine hydrochloride (Sigma Aldrich ...
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No products found
because this supplier's products are not listed.
Quote:
... anti-Hcp (against synthetic peptide, Eurogentec #1510528 (35)) ...
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No products found
because this supplier's products are not listed.
Martin Kollmar, et al.,
bioRxiv - Cell Biology 2019
Quote:
... 2016) using anti-GFP (Living Colors A.v. peptide antibody, rabbit polyclonal, 1 mg/ml; TakaraBio/Clontech) primary antibody and horseradish peroxidase linked anti-rabbit IgG (from donkey ...
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No products found
because this supplier's products are not listed.
Omri M. Finkel, et al.,
bioRxiv - Microbiology 2019
Quote:
... 0.375 μl mixed rRNA gene-blocking peptide nucleic acids (PNAs; 1:1 mix of 100 μM plastid PNA and 100 μM mitochondrial PNA; PNA Bio), 0.5 μl Kapa dNTPs ...
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No products found
because this supplier's products are not listed.
Andrej Trauner, et al.,
bioRxiv - Microbiology 2018
Quote:
... we used trypsin to digest proteins into peptides and then desalted them using C18 columns (The Nest Group). The cleaned up peptides were re-suspended in MS buffer (2% v/v acentonitrile ...
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No products found
because this supplier's products are not listed.
Xiangying Guan, et al.,
bioRxiv - Biochemistry 2017
Quote:
... 250 μM fluorolabeled peptide (FdL2 peptide, Enzo Life Sciences Farmingdale, NY, USA) with varying concentrations of DHP1 (0-100 μM ...
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No products found
because this supplier's products are not listed.
Quote:
Modified histone peptide arrays (Active Motif) were blocked with 5% dried milk in TBS-T (10 mM Tris-HCl pH 7.6 ...
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No products found
because this supplier's products are not listed.
Quote:
... ∼2.5 mg of the labelled peptide mixture were used to enrich for phospho-peptides via immobilized metal affinity chromatography (IMAC) using magnetic beads (Qiagen, Valencia, CA), according to the published method [113] ...
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No products found
because this supplier's products are not listed.
C López-Haber, et al.,
bioRxiv - Cell Biology 2020
Quote:
... OT-II peptide OVA323-339 (ISQAVHAAHAEINEAGR) was from InvivoGen (San Diego, CA) and kindly provided by Paula Oliver (Children’s Hospital of Philadelphia ...