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No products found
because this supplier's products are not listed.
Jordan P. Lewandowski, et al.,
bioRxiv - Genomics 2019
Quote:
... into a modified Tet-On pTRE2pur vector (Clontech, 631013) in which the bGlobin-intron was removed ...
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No products found
because this supplier's products are not listed.
Quote:
... using an ECM 830 Electroporator (BTX-Harvard Apparatus). Dilutions of transformed yeast were then plated on –Trp dropout medium as single colonies to obtain an estimate of library diversity.
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No products found
because this supplier's products are not listed.
Fanli Meng, et al.,
bioRxiv - Developmental Biology 2019
Quote:
... was modified to replace the original Tet-ON promoter [26] with a synthetic (GeneArt, Thermo Fisher, NZ) Dox-inducible PTRE3G promoter that provides very low basal expression and high maximal expression after induction [27] ...
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No products found
because this supplier's products are not listed.
Kevin Kleffman, et al.,
bioRxiv - Cancer Biology 2019
Quote:
Tet-pLKO-puro was purchased from Addgene (21915). sh-RNAs were cloned as previously described (https://mcmanuslab.ucsf.edu/protocol/cloning-small-hairpins-lentiviral-vectors ...
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No products found
because this supplier's products are not listed.
Shohei Kitano, et al.,
bioRxiv - Molecular Biology 2018
Quote:
HEK293T cells were cultured in DMEM containing 10% FBS at 37°C under a 5% CO2 atmosphere and transfected with 830 ng of plasmid using 2.5 μl FuGENE-HD transfection reagent (Promega) in 12-well plates.
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No products found
because this supplier's products are not listed.
Quote:
... 10% (v/v) tet-free FBS (TH. Geyer) and 200µg/mL G418 (Sigma). Cells were regularly tested for mycoplasma contamination.
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No products found
because this supplier's products are not listed.
Raffaella Soldi, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... and supplemented with 10% TET free FBS (Corning) and 1% penicillin/streptomycin (Gibco) ...
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No products found
because this supplier's products are not listed.
Quote:
... supplemented with 10% tet-tested FBS (Atlanta Biologicals) and penicillin-streptomycin (100 u/ml ...
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No products found
because this supplier's products are not listed.
Quote:
The Tet promoter-based Hughes Collection (yTHC) of yeast strains were purchased from GE Healthcare Dharmacon ...
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No products found
because this supplier's products are not listed.
Susanna Sabin, et al.,
bioRxiv - Genomics 2019
Quote:
... The two reactions were purified and eluted in 25 μl of TET each over MinElute columns (Qiagen), then assessed for efficiency using a real-time qPCR assay targeting the IS5 and IS6 sequences in the indexing primers ...
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No products found
because this supplier's products are not listed.
Quote:
We modified the NEBNext Ultradirectional (NEB) library preparation protocol to use 96 Barcodes from BIOOScientific added by ligation ...
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No products found
because this supplier's products are not listed.
Rachel L. French, et al.,
bioRxiv - Biochemistry 2018
Quote:
... in a modified Mini Trans-Blot Cell (Bio-Rad) at 4°C ...
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No products found
because this supplier's products are not listed.
Quote:
... in Dulbecco’s Modified Eagle’s Medium (Lonza) supplemented with 10% (v/v ...
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No products found
because this supplier's products are not listed.
Quote:
... followed by modified trypsin (Roche) 20 ...
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No products found
because this supplier's products are not listed.
Quote:
... Tet-ATTO532 (Jena Bioscience GmbH, Jena, Germany) or AF647 (Thermo Fisher Scientific ...
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No products found
because this supplier's products are not listed.
Quote:
... Viruses were assembled using a modified helper-free system (Stratagene) as serotype 2/1 (rep/cap ...
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No products found
because this supplier's products are not listed.
Quote:
... with 10% Tet-free Fetal Bovine Serum (FBS) (Omega Scientific), 1% penicillin streptomycin (ATCC ...
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No products found
because this supplier's products are not listed.
Catarina A. Marques, et al.,
bioRxiv - Genomics 2020
Quote:
... the Tet-induced cells were filtered (Filcon Cup-type filter, 50 μm mesh, BD™ Medimachine) into 5 ml polystyrene round-bottom tubes (BD Falcon) ...
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No products found
because this supplier's products are not listed.
Rikai Sawafuji, et al.,
bioRxiv - Molecular Biology 2018
Quote:
All primers were modified to include a subset of Nextera XT (Illumina) adapters ...
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No products found
because this supplier's products are not listed.
Arun M. Unni, et al.,
bioRxiv - Cancer Biology 2017
Quote:
... or FBS that was tested to be Tet-free (VWR Life Science Seradigm), 10mM HEPES (Gibco ...
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No products found
because this supplier's products are not listed.
Zack Zurawski, et al.,
bioRxiv - Neuroscience 2018
Quote:
... using a modified TIRF laser launch (Olympus IX2). Coverslips were 0.17 mm borosilicate glass ...
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No products found
because this supplier's products are not listed.
Quote:
... equipped with an Orius SC200-830 CCD camera (Gatan).
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No products found
because this supplier's products are not listed.
Essam A. Assali, et al.,
bioRxiv - Physiology 2018
Quote:
Modified Columbus (PerkinElmer) image analysis software was used to calculate intensity of the fluorescent probe.
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No products found
because this supplier's products are not listed.
Quote:
... in Modified Tenneille’s Special Recipe 1 (mTeSR1, STEMCELL Technologies), supplemented with 1% Penicillin/Streptomycin (Gibco ...
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No products found
because this supplier's products are not listed.
Quote:
... bisulfite-modified genomic DNA (Quick-DNA Miniprep Plus Kit, Zymo Research ...
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No products found
because this supplier's products are not listed.
Quote:
... to express the Tet repressor and selected with 10 μg/ml Blasticidin (Invivogen, San Diego, CA, USA). Multiple stable clones were isolated ...
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No products found
because this supplier's products are not listed.
Oguzhan Begik, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... All modified NTPs were purchased from TriLink. The sequences included in the short unmodified dataset (UNM-S) ...
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No products found
because this supplier's products are not listed.
Utku M. Sonmez, et al.,
bioRxiv - Physiology 2020
Quote:
... A modified Nikon Ti-E (Nikon instruments, Japan) was used for these studies ...
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No products found
because this supplier's products are not listed.
Vivek N. Prakash, Matthew S. Bull, Manu Prakash,
bioRxiv - Biophysics 2019
Quote:
... adhaerens are placed on a base of a modified Stereomicroscope (Leica S9 D) stand ...
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No products found
because this supplier's products are not listed.
Kaveh Daneshvar, et al.,
bioRxiv - Molecular Biology 2019
Quote:
Modified Histone arrays (Active Motif) were used as described (Saltzman et al. ...
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No products found
because this supplier's products are not listed.
Yuancheng Lu, et al.,
bioRxiv - Molecular Biology 2019
Quote:
... Induction of Tet-On AAV9 system systemically was performed by administration of doxycycline (1 mg/ml) (USP grade, MP Biomedicals 0219895505) in the drinking water.
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No products found
because this supplier's products are not listed.
Quote:
... The fragment was cloned into modified pET21b (Novagen, Merck, USA) using restriction enzyme sites BamHI and SalI ...
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No products found
because this supplier's products are not listed.
Quote:
... Santa Clara) and a modified MIMMS microscope manufactured by Sutter Instrument (Novato, CA). We replaced the scan and tube lenses (respectively ...
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No products found
because this supplier's products are not listed.
Quote:
... and tetracycline (Tet) at 10 μg/mL (Amresco and Acros Organics). Cadmium chloride was used at 0.1-0.3 mM to select for pJC1111 transductants ...
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No products found
because this supplier's products are not listed.
Quote:
... formulation modified from Irvine Scientific, Santa Ana ...
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No products found
because this supplier's products are not listed.
Quote:
... the tet-sacB selection/counterselection cassette was amplified using the appropriate oligos supplying ∼50 bp flanking homology sequences using Econotaq (Lucigen Middleton, WI) according to manufacturer’s instructions ...
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No products found
because this supplier's products are not listed.
Quote:
HEK 293T cells were cultured in Dulbecco’s modified Eagle’s medium (Euroclone) supplemented with 10% fetal bovine serum (Euroclone) ...
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No products found
because this supplier's products are not listed.
Quote:
... the conjugates were purified using a modified Agencourt AMPure XP Kit (Beckman Coulter) and eluted in 20 μL 0.1x IDTE (IDT) ...
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No products found
because this supplier's products are not listed.
Jordan F. Hastings, et al.,
bioRxiv - Cancer Biology 2019
Quote:
The cisplatin modified DNA (ab103261) and γH2A.X (ab26350) antibodies were from Abcam (Cambridge, USA). The phospho-STAT3 S727 (#9134) ...
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No products found
because this supplier's products are not listed.
Quote:
... The modified BAC construct was purified with a NucleoBond Xtra Midi kit (Macherey-Nagel) and injected into the pronuclei of fertilized one-cell mouse eggs derived from the breeding of BDF1 and C57BL/6N mice ...
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No products found
because this supplier's products are not listed.
Quote:
... or a custom-modified PTI QM-1 fluorometer (Photon Technology International, New Brunswick, NJ) [9] ...
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No products found
because this supplier's products are not listed.
Athea Vichas, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... NIH3T3 and HeLa-H2B cells were cultured in Dulbecco's Modified Eagle's Medium (DMEM, Genesee Scientific) supplemented with 10% FBS (Sigma) ...
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No products found
because this supplier's products are not listed.
Quote:
... Recording pipettes were pulled on a PP-830 vertical puller (Narishige) from borosilicate glass pipettes (1B150F-4, World Precision Instruments). Patch pipettes were pulled to resistances of 5-7 MΩ for hair cells ...
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No products found
because this supplier's products are not listed.
Quote:
The DNA sequence encoding a reverse tet transactivator protein (rtTA) was synthesized (Genscript) and subcloned into lentiviral vector pLentis-CMV-MCS-IRES-Bsd between BamHI and XhoI sites ...
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No products found
because this supplier's products are not listed.
Syuan-Ming Guo, et al.,
bioRxiv - Bioengineering 2017
Quote:
... Amino-modified phalloidin (Bachem AG, Bubendorf, Switzerland) was conjugated using the protocol described above with an extra step of HPLC purification to separate unreacted thiolated ssDNA and ssDNA-conjugated peptides ...
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No products found
because this supplier's products are not listed.
Quote:
... 10 μm yellow-green fluorescent carboxylate modified polystyrene beads (Polysciences) were functionalized with BSA ...
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No products found
because this supplier's products are not listed.
Patricia J. Ahl, et al.,
bioRxiv - Immunology 2020
Quote:
... The modified T cell panel included CCR7 (clone G043H7, Biolegend, BV650, 353134), CD45RA (cloneHI100 ...
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No products found
because this supplier's products are not listed.
Zharko Daniloski, et al.,
bioRxiv - Genetics 2020
Quote:
... All cells were cultured in D10 media: Dulbecco’s Modified Eagle Medium (Caisson Labs) supplemented with 10% Serum Plus II Medium Supplement (Sigma-Aldrich) ...
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No products found
because this supplier's products are not listed.
Ali Isbilir, et al.,
bioRxiv - Biophysics 2017
Quote:
... Cells were cultured in DMEM (Dulbecco’s modified Eagle’s medium) (PAN Biotech, Aidenbach, Germany), supplemented with 4,5 g/L Glucose ...
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Trypsin, treated with L-(tosylamido-2-phenyl) ethyl chloromethyl ketone to inhibit contaminating...
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4x25 ug, USD $82.0/1ea
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Quote:
... tissue was dissociated using a modified version of Worthington Papain Dissociating System® (Worthington Biochemical Corporation, LK003150). After incubating with papain for 45 min at 37°C on a rocking platform ...