-
No products found
because this supplier's products are not listed.
Quote:
... within a small volume of Opti-MEM (200 μL; Invitrogen) according to the manufacturer’s instructions ...
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No products found
because this supplier's products are not listed.
David Thaller, et al.,
bioRxiv - Cell Biology 2019
Quote:
... collected by centrifugation and resuspended in a small volume of freezing buffer (20 mM HEPES, pH 7.4, 1.2% polyvinylpyrrolidone and protease inhibitor (Sigma)(Oeffinger et al. ...
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No products found
because this supplier's products are not listed.
Quote:
... samples were concentrated using a centrifugal evaporator Speed Vac® to a final volume of 5 μl and we started the TruSeq Small RNA Sample Preparation Kit (Illumina) protocol ...
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No products found
because this supplier's products are not listed.
Quote:
... small-volume 384-well plate (# 784101, Greiner Bio-one) according to the manufacturer’s protocol ...
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No products found
because this supplier's products are not listed.
Quote:
... Total RNA was quantified (Take3 micro-volume plate of the Epoch spectrophotometer; BioTek®, USA). The RNA integrity was evaluated by electrophoresis on a denaturing agarose gel stained with GelRed Nucleic Acid Gel Stain (Uniscience ...
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No products found
because this supplier's products are not listed.
Quote:
... Small RNA-seq libraries were prepared using NEBNext small RNA library preparation kit (NEB E7330) as previously described (Shivram and Iyer 2018).
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No products found
because this supplier's products are not listed.
Nicholas Rhoades, et al.,
bioRxiv - Molecular Biology 2019
Quote:
... Resultant small RNAs were purified using 1.8 volumes of Agencourt RNAClean XP Beads (Beckman Coulter, NC0068576) and three volumes of isopropanol ...
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No products found
because this supplier's products are not listed.
Quote:
... The entire sample was then added to a 250 μL small volume pulled point class insert (Agilent Part No: 5183-2085) to facilitate autosampler needle access ...
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No products found
because this supplier's products are not listed.
Kenneth F. Rodrigues, et al.,
bioRxiv - Genetics 2016
Quote:
... the concentration of DNA was assessed using a micro-volume UV-Vis Spectrophotometer (GE Healthcare Life Sciences) and the final concentration was adjusted to 50 ng/μl using sterile nuclease free water ...
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No products found
because this supplier's products are not listed.
Shijian Zhang, et al.,
bioRxiv - Microbiology 2018
Quote:
... The supernatant was collected and was mixed with a small volume of preequilibrated Ni-nitrilotriacetic acid (NTA) beads (Qiagen) for 2 h on a rocking platform at 4°C ...
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No products found
because this supplier's products are not listed.
John Kealy, et al.,
bioRxiv - Neuroscience 2019
Quote:
... using a small volume insulin syringe (BD Micro - Fine™ + 0.3ml Insulin Syringe Demi) ...
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No products found
because this supplier's products are not listed.
Quote:
... with the MagNA Pure 96 DNA and viral NA Small Volume Kit (Roche; Cat ID. 06543588001), and the MagNa Pure 96 System Fluid (Roche ...
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No products found
because this supplier's products are not listed.
Quote:
... A SmartSpec 3000 spectrophotometer (Bio-Rad) was used to measure the absorbance at 530 and 657 nm ...
-
No products found
because this supplier's products are not listed.
Javier Valdes-Aleman, et al.,
bioRxiv - Neuroscience 2019
Quote:
... on a small Sylgard (Dow Corning) plate.
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No products found
because this supplier's products are not listed.
Quote:
... A spectrophotometer (SpectraMax 190, Molecular Devices) was used to determine the optical density.
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No products found
because this supplier's products are not listed.
Quote:
... with a spectrophotometer (Tecan Infinite F500). Cell numbers were normalized to the desired initial concentrations using a three-point linear calibration between OD 600 nm and direct cell counts determined with a Guava easyCyte Benchtop Flow Cytometer for each strain.
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No products found
because this supplier's products are not listed.
Zhe Lyu, et al.,
bioRxiv - Microbiology 2017
Quote:
... Then small amounts of trypsin solution (Promega, 0.01μg μL−1 in 20 mM ammonium bicarbonate ...
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No products found
because this supplier's products are not listed.
Heba Ijaz, et al.,
bioRxiv - Cancer Biology 2019
Quote:
... DNA and RNA quantity and quality was assessed by PerkinElmer DropletQuant UV-VIS spectrophotometer (PerkinElmer, USA) and an Agilent 4200 TapeStation (Agilent ...
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No products found
because this supplier's products are not listed.
Quote:
... 20 larvae were transferred into a small plastic cup (VWR HDPE Multipurpose Containers ...
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No products found
because this supplier's products are not listed.
Melanie V. Halbach, et al.,
bioRxiv - Neuroscience 2018
Quote:
... Protein concentration was determined with a Spectrophotometer (Eppendorf) using 5x Bradford Reagent (Roti-Quant ...
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No products found
because this supplier's products are not listed.
Quote:
... ethanol precipitation (3 volumes of 100 % EtOH, with 1/10th volume of 3M NaAc ...
-
No products found
because this supplier's products are not listed.
Daniel Duzdevich, et al.,
bioRxiv - Synthetic Biology 2020
Quote:
... purified with a ZR small-RNA PAGE Recovery Kit (Zymo Research), eluted in 30 μl TE ...
-
No products found
because this supplier's products are not listed.
Quote:
... Single-end small RNA libraries were prepared using the SMARTer kit (Clontech). Up to 12 barcoded libraries from a single species were run on a single Illumina HiSeq 2500 lane.
-
No products found
because this supplier's products are not listed.
Kishor Jakkala, et al.,
bioRxiv - Microbiology 2019
Quote:
... volumes of 95% ice-cold ethanol (Merck Millipore) was added and mixed vigorously for 15 to 20 times by inverting the Eppendorf tubes ...
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No products found
because this supplier's products are not listed.
Quote:
... using a fluorescence spectrophotometer (BMG Labtech, Ortenberg, Germany) set to excitation 490nm ...
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No products found
because this supplier's products are not listed.
Quote:
... A small animal pneumotachometer (PTM Type HSE-73-0980, Harvard Apparatus, Holliston, MA) connected directly to the tracheal tube measured air flow ...
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No products found
because this supplier's products are not listed.
Quote:
... A small volume (20 – 40 nl) of virus was injected (WTB and KAE: Nanoject II, Drummond Scientific, Broomall ...
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No products found
because this supplier's products are not listed.
Quote:
... 1 volume of zirconia beads (BioSpec 11079105z) were added and cells were lysed with FastPrep-24 5g (MP biomedicals 116005500 ...
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No products found
because this supplier's products are not listed.
Andreas J. Genewsky, et al.,
bioRxiv - Neuroscience 2018
Quote:
... in combination with a small rodent rectal temperature probe (WPI Inc. #RET-3) and a small heating-pad (15×10 cm ...
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No products found
because this supplier's products are not listed.
Quote:
... and small airway growth medium (SAGM) medium (Lonza) containing 5% fetal calf serum (FCS ...
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No products found
because this supplier's products are not listed.
Sarah Gift Witto, et al.,
bioRxiv - Microbiology 2019
Quote:
... PCR amplification was performed in 25µl volumes with 2Χ Ready-mix (Bioline, UK) (volume 12.5µl ...
-
No products found
because this supplier's products are not listed.
Quote:
... Small interfering RNAs (siRNAs) targeting GYS2 were purchased from Santa Cruz (SC-69704). Others were designed from Shanghai GenePharma Co ...
-
No products found
because this supplier's products are not listed.
Quote:
... The size selected RNA was then used to construct small RNA libraries using the NEXTflex Small RNA Library Prep kit v3 (Bioo Scientific) and sequenced on the Illumina HighSeq instrument at the Kansas University Genome Sequencing Core ...
-
No products found
because this supplier's products are not listed.
Quote:
... CGRP (marker of small peptidergic neurons; 1:1000, ab81887, Abcam, UK), IB4 (conjugated to Alexa Fluor 647 ...
-
No products found
because this supplier's products are not listed.
Miguel Alejandro Lopez-Ramirez, et al.,
bioRxiv - Biochemistry 2019
Quote:
... Low volume dispensing plates (Labcyte #LP-0200) were assembled using an Agilent BioCell work station (Santa Clara ...
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No products found
because this supplier's products are not listed.
Quote:
... Libraries for small RNA sequencing were prepared using the CleanTag small RNA library prep kit (Trilink) according to manufacturer’s instructions.
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No products found
Quote:
... small-molecule inhibitors CHIR99021 (CH, 3 $M- Axon Medchem) and PD0325901 (PD ...
-
No products found
Jacob P. Keller, et al.,
bioRxiv - Bioengineering 2019
Quote:
... cut into small pieces and dissociated in papain enzyme (Worthington, #PAP2) in dissection buffer for 30 minutes at 37°C ...
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No products found
because this supplier's products are not listed.
Quote:
... a small amount of 0.5 mm zirconium oxide beads (Next Advance, ZrOB05) was added to the collected worms ...
-
No products found
because this supplier's products are not listed.
Quote:
... Each reward was a small volume (5-10 μl) of an 8% solution of soy-based infant formula (Similac, Abbott, IL) delivered by a peristaltic pump (Harvard Apparatus ...
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No products found
because this supplier's products are not listed.
Quote:
... A small round coverslip (Electron Microscopy Sciences, # 72230-01) was immediately placed on top and very slightly pressed ...
-
No products found
because this supplier's products are not listed.
Quote:
... The “volume render” function of EZ-C1 software (Nikon) was used to collapse the stack into a single image ...
-
No products found
because this supplier's products are not listed.
Etienne Becht, et al.,
bioRxiv - Immunology 2020
Quote:
... a 10-fold volume of Cell Staining Buffer (Biolegend) was added to neutralize any unbound dye and cells were centrifuged at 300g for 5 minutes ...
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No products found
because this supplier's products are not listed.
Quote:
Electronic absorption spectra were recorded on SPECORD UV/VIS spectrophotometer (Carl Zeiss, Jena).
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No products found
because this supplier's products are not listed.
Nicholas Rhoades, et al.,
bioRxiv - Molecular Biology 2019
Quote:
... Purified small RNAs were treated with RNA 5’-polyphosphatase (Lucigen, RP8092H) to remove 5’ phosphates ...
-
No products found
because this supplier's products are not listed.
Quote:
... The medium was supplemented with small molecule inhibitors: 500nM A 83-01 (Tocris), a TGF-β inhibitor ...
-
No products found
because this supplier's products are not listed.
Quote:
... immortalized MEFs (transduced with large T and small T expressing lentivirus (Plasmid #22298, Addgene) were transduced with an H2B-Neon expressing lentivirus (pLV-H2B-Neon-ires-Puromycin)74,75 ...
-
No products found
because this supplier's products are not listed.
Quote:
... Cultures in chemostat and segregostat mode have been performed in lab-scale stirred bioreactor (Biostat B-Twin, Sartorius. Total volume: 2L; working volume: 1L). For the batch phase ...
-
No products found
because this supplier's products are not listed.
Quote:
... coluzzii were pooled at equal volume and sheared using an S220 ultrasonicator (Covaris) to produce DNA fragments 800-1000 bp in length ...
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No products found
because this supplier's products are not listed.
Till Tiso, et al.,
bioRxiv - Bioengineering 2020
Quote:
... 1.6 ml volume cuvette (Sarstedt, Germany). 400 µl of phenol solution (per 100 ml of deionized water ...