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No products found
because this supplier's products are not listed.
Chiara Diquigiovanni, et al.,
bioRxiv - Cell Biology 2018
Quote:
... were subsequently separated on 10% sodium dodecyl sulfate polyacrylamide electrophoresis (SDS-PAGE) gels or on 4-20% pre- cast SDS-PAGE gels (Biorad). Gels were then electro-transferred onto nitrocellulose membranes (Trans-Blot Turbo Transfer System ...
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No products found
because this supplier's products are not listed.
Quote:
... SDS-PAGE was performed with 10% gels (Invitrogen) under reducing and non-reducing conditions ...
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No products found
because this supplier's products are not listed.
Zhong Tian, et al.,
bioRxiv - Immunology 2018
Quote:
Tricine-SDS PAGE gel kit was purchased from Sigma; CCK-8 kit ...
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No products found
because this supplier's products are not listed.
Quote:
... Samples were run on 4-12% SDS-PAGE gels (Mini-Protean TGX Precast gel, Bio-Rad) and subsequently transferred to nitrocellulose membranes using the Trans-Blot Turbo instrument (Bio-Rad) ...
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No products found
because this supplier's products are not listed.
Claire Hamilton, et al.,
bioRxiv - Immunology 2020
Quote:
... SDS-PAGE gels were transferred to nitrocellulose membranes (GE Life Sciences) and subsequently blocked in 5% milk solution in TBS-Tween (0.05%) ...
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No products found
because this supplier's products are not listed.
Tanya Lasitza Male, et al.,
bioRxiv - Biophysics 2020
Quote:
... SDS-PAGE using a 4-12% Bis-Tris gel (Expedeon) was performed and transferred on a PVDF membrane (Biorad) ...
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No products found
because this supplier's products are not listed.
Quote:
... reduced samples were resolved on 10% SDS-PAGE gels and blotted onto PVDF membranes (Merck). Membranes were blocked with 5% non-fat milk in PBS with 0.05% Tween-20 (Merck ...
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No products found
because this supplier's products are not listed.
Jason Buehler, et al.,
bioRxiv - Molecular Biology 2019
Quote:
Lysates were separated by electrophoresis on precast 12% Tris-Bis SDS-PAGE gel (Genscript) or 4-20% precast gels (BioRad) ...
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No products found
because this supplier's products are not listed.
Quote:
... Samples were loaded onto 10% or 12% Tris Tricine SDS-PAGE gels (Abcam) and fractionated at 180 V for 30 to 70 minutes in the presence of SDS running buffer (Abcam ...
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No products found
because this supplier's products are not listed.
N. Cumbal, MD. Cole,
bioRxiv - Cancer Biology 2019
Quote:
... Resulting eluates and supernatants were loaded on a 10% SDS-PAGE gel and then probed with the MYC (N262) primary antibody (Santa Cruz).
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No products found
because this supplier's products are not listed.
Leticia Lemus, Zrinka Matić, Veit Goder,
bioRxiv - Cell Biology 2019
Quote:
... followed by SDS-PAGE and Western Blotting using anti-GFP antibody (Roche), HRP-conjugated anti-mouse secondary antibody (Roche ...
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No products found
because this supplier's products are not listed.
Huadong Jiang, et al.,
bioRxiv - Cell Biology 2019
Quote:
... Proteins were resolved by 12.5% SDS-PAGE gel and then transferred to nitrocellulose membranes (Pall Corporation). Membranes were blocked with 5% non-fat milk in TBST (0.1% Tween 20 ...
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No products found
because this supplier's products are not listed.
Arijita Chakraborty, et al.,
bioRxiv - Genomics 2019
Quote:
... before analysis on 8% SDS-PAGE gels and western blotting using anti-FMRP (Cell signaling, 1:500), anti-GAPDH (Thermo scientific ...
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No products found
because this supplier's products are not listed.
Quote:
... Same volume of samples were loaded on 4-16% gradient SDS-PAGE gels and analyzed by western blot analysis using EGFP (JL-8; Clontech), penta-HIS (QIAGEN ...
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No products found
because this supplier's products are not listed.
Quote:
... and each peak collected was submitted to SDS-PAGE gel and mass spectrometry (Agilent 6530 QTOF (Agilent Technologies Inc ...
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No products found
because this supplier's products are not listed.
Quote:
... Protein was detected via SDS-PAGE and Western blotting using an Alexa 647-conjugated anti-His-tag antibody (QIAGEN) and a Typhoon Imager (GE Healthcare) ...
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No products found
because this supplier's products are not listed.
Yiqing Zhao, et al.,
bioRxiv - Cancer Biology 2017
Quote:
SDS-PAGE gel bands containing paxillin protein were subjected to in-gel digestion with sequencing grade modified trypsin (Promega, WI). Briefly ...
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No products found
because this supplier's products are not listed.
Quote:
... The reactions were quenched by the addition of SDS-PAGE loading buffer and analyzed by 10% Tricine-SDS-PAGE and stained with Coomassie Brilliant Blue G-250 (VWR).
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No products found
because this supplier's products are not listed.
Quote:
... Proteins were prepared by adding 6X reducing Laemmli buffer and boiling for 5 min and a total of 5 μg of total protein extracts or 0.5 μg of purified histones were separated on denaturing 10% or 15% SDS-PAGE gels based on the target size and proteins were transferred onto PVDF transfer membranes (0.45 μm, Perkin Elmer, Waltham, MA). Membranes were probed with the following primary antibodies ...
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No products found
because this supplier's products are not listed.
Joshua T. Rose, et al.,
bioRxiv - Cancer Biology 2019
Quote:
... 8% SDS resolving gels and 4% stacking gels were prepared in-house (National Diagnostics, Atlanta, GA). Cell harvests were resuspended in RIPA buffer and incubated on ice for 30min ...
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No products found
because this supplier's products are not listed.
Quote:
Size selection using PAGE gels was recommended by three of the manufacturers (Illumina, NEXTflex, and NEB kits) and was performed for these kits for better comparisons ...
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No products found
because this supplier's products are not listed.
Quote:
... Purified protein was separated using 15% SDS-PAGE gels and visualized using an Odyssey infrared imager (LI-COR Biosciences).
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No products found
because this supplier's products are not listed.
Quote:
... Equal amounts of lysate were loaded on an SDS-PAGE gel and subsequent western blotting was carried out with primary antibodies against FMRP (1:1000, cat# 6B8, BioLegend), GAPDH (1:1000 ...
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No products found
because this supplier's products are not listed.
Gulzhan Raiymbek, et al.,
bioRxiv - Biochemistry 2019
Quote:
... Proteins were separated through SDS-PAGE and transferred to PVDF membrane followed by incubation with anti-MBP monoclonal antibody (E8032S, NEB) and M2 Flag antibody (A8592 ...
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No products found
because this supplier's products are not listed.
Quote:
... Linear DNA corresponding to a Fd gene containing a single transposon (1,700 bp) was excised from the gel and purified using a Gel DNA recovery kit (Zymo Research). The Fd-transposon gene fusions were cloned into pFd007-staging using Golden Gate Assembly40 ...
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No products found
because this supplier's products are not listed.
Quote:
... The proteins in the lysates were separated by SDS-PAGE and transferred to polyvinylidene difluoride membranes (BD Biosciences). The phosphorylation of the ERBBs and ERK was detected on the membranes with anti-pERBB1–B4 and anti-ppERK primary antibodies ...
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No products found
because this supplier's products are not listed.
Tao Fan, et al.,
bioRxiv - Cell Biology 2018
Quote:
... Fifty micrograms of protein was subjected to SDS-polyacrylamide gel electrophoresis (12%PAGE; Amresco) and transferred to a polyvinylidene fluoride membrane (Millipore ...
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No products found
because this supplier's products are not listed.
Quote:
... the single band was purified from a gel with a NucleoSpin Gel and PCR cleanup kit (Macherey-Nagel) and its concentration determined ...
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No products found
because this supplier's products are not listed.
Wei Song Ong, Seth Madlon-Kay, Michael L. Platt,
bioRxiv - Neuroscience 2018
Quote:
All single-unit recordings were made using single tungsten microelectrodes (FHC). In each recording session ...
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No products found
because this supplier's products are not listed.
Ramin Raouf, et al.,
bioRxiv - Neuroscience 2017
Quote:
... The samples were resolved using SDS-PAGE on 8 - 12% gels and transferred to PVDF or nitrocellulose filters (R&D systems). The membrane was blocked with 0.1% Tween-20 in PBS ...
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No products found
because this supplier's products are not listed.
Jill E. Hunter, et al.,
bioRxiv - Cancer Biology 2018
Quote:
... and single cell suspension collected in DMEM (Lonza) supplemented with 10% FBS ...
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No products found
because this supplier's products are not listed.
LJ Davison, et al.,
bioRxiv - Genetics 2018
Quote:
SDS-PAGE gel electrophoresis was undertaken using 4-20% pre-cast Tris-Glycine gels (NuSep, Homebush, Australia) and PVDF blotting membrane ...
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No products found
because this supplier's products are not listed.
Quote:
... Purified mAbs were analyzed for purity using 12% SDS-PAGE and concentration was determined by Take3 (BioTek instruments).
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No products found
because this supplier's products are not listed.
Quote:
... Fractions were analysed by SDS-PAGE and pure fractions concentrated by centrifugation in Vivaspin 10,000 MWCO (Sartorius, Goettingen, Germany) proceeding crystallisation.
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No products found
because this supplier's products are not listed.
Xiangduo Kong, et al.,
bioRxiv - Molecular Biology 2019
Quote:
... urea-PAGE stained by GelRed (Biotium) and was also used for RT-qPCR ...
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No products found
because this supplier's products are not listed.
Yu-Yu Cheng, et al.,
bioRxiv - Systems Biology 2020
Quote:
... Single cells were imaged by Nikon Eclipse Ti Microscope with 40X magnification ...
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No products found
because this supplier's products are not listed.
Quote:
... single cells were plated in ultralow attachment plates (Corning) at a density of 2×104 viable cells/ml in DMEM/F-12 medium supplemented with insulin (Clonetics ...
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No products found
because this supplier's products are not listed.
Quote:
... hiPSCs were passed as single cells with Accutase (StemCell Technologies) and were plated on Matrigel-coated (Corning ...
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No products found
because this supplier's products are not listed.
Quote:
... Single cells were visualized with an inverse microscope (Olympus IX71) equipped with a differential interference contrast ...
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No products found
because this supplier's products are not listed.
Hiroshi Ochiai, et al.,
bioRxiv - Systems Biology 2019
Quote:
... Single-cell sorting was performed using MoFlo Astrios (Beckman Coulter). Recent studies of scRNA-Seq using mESCs have suggested that genes related to the cell cycle demonstrate considerable heterogeneity in expression [44] ...
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No products found
because this supplier's products are not listed.
Cemal Erdem, et al.,
bioRxiv - Systems Biology 2020
Quote:
... The gel was run in SDS running buffer (100 mL 10X Tris-Glycine-SDS buffer (IBI Scientific #IB01160) + 900 mL milli-Q water ...
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No products found
because this supplier's products are not listed.
Annika Andersson, et al.,
bioRxiv - Biochemistry 2019
Quote:
... SDS–PAGE and Western blot analysis with a 1:3333 dilution of a rabbit anti-GFP antibody (Rockland, USA) were carried out ...
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No products found
because this supplier's products are not listed.
Quote:
Single Cell Gel Electrophoresis (SCGE) was carried out using the CometAssay® ES II kit (Trevigen) according to the manufacturer’s instructions ...
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No products found
because this supplier's products are not listed.
Quote:
... single cells from EMS- and sham-treated populations were isolated via FACS and recovered on YPD agar (2% dextrose ...
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No products found
because this supplier's products are not listed.
Quote:
... Single channel analysis was performed using Clampfit 10.0 single channel search (Molecular Devices). Dwell times were calculated using “single-channel search” using threshold crossing.
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Prepared by a method developed at Worthington in which denatured, single-stranded calf thymus...
Cat# LS01132,
5 gm, USD $138.0/1ea
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Quote:
... Single-cell suspensions were prepared using Papain (Worthington) with 30min enzymatic digestion ...
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No products found
because this supplier's products are not listed.
Quote:
Three SD rats (Charles River, Hollister, CA) were immunized with 100 µg OVA (Sigma-Aldrich ...
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No products found
because this supplier's products are not listed.
Quote:
... Single cells were manually isolated with a micromanipulator (Eppendorf, PatchMan NP2) as previously described (9) ...
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No products found
because this supplier's products are not listed.
Quote:
... the single cell suspension was filtered through 70μm filter (Miltenyi Biotec) and cleaned of debris by centrifugation through 1 ml FBS at 800g for 10min ...
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No products found
because this supplier's products are not listed.
Petr Dvořák, et al.,
bioRxiv - Plant Biology 2020
Quote:
... and a spinning disk microscope (Cell Observer, SD, Carl Zeiss, Germany). Image acquisition was done with 20× (0.8 NA ...