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No products found
because this supplier's products are not listed.
Martijn R. Molenaar, et al.,
bioRxiv - Biochemistry 2019
Quote:
Retinol (Sigma) was dissolved in ethanol (30 mM ...
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No products found
because this supplier's products are not listed.
Quote:
... 0.3 μl ssDNA Binding Protein (Affymetrix, Santa Clara, USA), 100 nM of each primer (reverse primer contains sample-specific DNA barcode) ...
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No products found
because this supplier's products are not listed.
Quote:
... insulin receptor (IR) and rat retinol binding protein-4 (RBP4) were obtained from Qiagen (Germany), Intron Biotechnology (Korea ...
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No products found
because this supplier's products are not listed.
James D. Warner, et al.,
bioRxiv - Biochemistry 2017
Quote:
... and TATA-binding protein (TBP; ab818) were from Abcam; Antibodies against eIF4G1 (RN002P ...
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No products found
because this supplier's products are not listed.
Quote:
His6-tagged proteins were purified by binding proteins to Ni-NTA columns (GE Healthcare) in buffer D (50 mM Tris pH 8.0 ...
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No products found
because this supplier's products are not listed.
Kimberly A Haupt, et al.,
bioRxiv - Developmental Biology 2019
Quote:
... PUF protein variants were fused to the LexA binding domain (BD), which was V5 tagged ...
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No products found
because this supplier's products are not listed.
Adam M. Lakusta, et al.,
bioRxiv - Plant Biology 2018
Quote:
For pre-binding of protein G magnetic beads (NEB, ON, Canada) to monoclonal mouse anti-FLAG M2 antibody (Sigma ...
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No products found
because this supplier's products are not listed.
Leonor Guedes da Silva, et al.,
bioRxiv - Microbiology 2019
Quote:
... The protein concentration was measured using the Bradford dye-binding protein assay (Bio-Rad), using known concentrations of bovine serum albumin as external standards.
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No products found
because this supplier's products are not listed.
Quote:
... CREB binding protein (CBP; Santa Cruz), cyclin A (Novocastra) ...
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No products found
because this supplier's products are not listed.
Christian Christensen, et al.,
bioRxiv - Cell Biology 2019
Quote:
... Sterile filtration was performed using a 0.45 μm low protein-binding funnel (Corning Incorporated, NY, USA) in a closed system ...
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No products found
because this supplier's products are not listed.
Quote:
... Protein-low binding tubes (Eppendorf) were used to make serial dilutions of BODIPY-cyclopamine.
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No products found
because this supplier's products are not listed.
Juliana Soares, et al.,
bioRxiv - Biophysics 2020
Quote:
... polyclonal antibody against brain lipid binding protein (BLBP) (Millipore, Merck KGaA, Germany), mouse antibody against nestin (Millipore ...
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No products found
because this supplier's products are not listed.
Jos R. Wendrich, et al.,
bioRxiv - Plant Biology 2018
Quote:
In vitro microtubule-binding assay was performed using Microtubule Binding Protein Spin-down Assay Kit (Cytoskeleton). Briefly ...
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No products found
because this supplier's products are not listed.
Noboru Ideno, et al.,
bioRxiv - Cancer Biology 2018
Quote:
... Nonspecific binding was blocked with serum free protein block (Dako, Glostrup, Denmark) before overnight incubation with primary antibodies listed in the Supplemental Table 1 ...
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No products found
because this supplier's products are not listed.
Simon J Moore, et al.,
bioRxiv - Synthetic Biology 2020
Quote:
... low-binding plate (Greiner). Reactions were measured as a triplicate technical repeat and at least repeated with cell-extracts prepared from two separate days ...
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No products found
because this supplier's products are not listed.
Quote:
... coli LexA DNA binding protein (Takara Bio USA, Inc.). Luciferase (firefly luciferase from Photinus pyralis ...
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No products found
because this supplier's products are not listed.
Mengjia Song, et al.,
bioRxiv - Cancer Biology 2019
Quote:
... or anti-RBP-Jκ (Cell Signaling Technology, 1:50) antibody was used to immunoprecipitate the chromatin in A549 and H460 cells transfected with shScramble or with shScramble ...
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No products found
because this supplier's products are not listed.
Quote:
... Protein-DNA binding specificity was determined by adding poly (dI-dC) (Roche) or/and 100-fold excess unlabeled DNA probe prior to the addition of labeled probe during the room temperature binding reaction ...
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No products found
because this supplier's products are not listed.
Quote:
... The supernatant was then transferred to a new low protein-binding microcentrifuge tube or 96-well low protein-binding plate (VWR, Cat# 951032921) and immediately processed for analysis (see below ...
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No products found
because this supplier's products are not listed.
Vítor Amorim-Silva, et al.,
bioRxiv - Molecular Biology 2018
Quote:
... or MBP binding protein coupled to agarose beads (MBP-Trap_A, Chromotek), respectively ...
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No products found
because this supplier's products are not listed.
Qiang Jiang, et al.,
bioRxiv - Developmental Biology 2020
Quote:
... and 200 ng purified recombinant mouse DLX2 protein in Gel Shift Binding Buffer (Promega). Unlabeled probe was added for ‘cold’ competition ...
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No products found
because this supplier's products are not listed.
A. Savina, et al.,
bioRxiv - Cell Biology 2020
Quote:
... HA-binding protein staining was achieved with 1/100 strepavidin HRP (Perkin Elmer, Massachusetts, USA) solution for 30 min at room temperature and 3,3’-diaminobenzidine (Liquid DAB + substrate Chromagen System ...
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No products found
because this supplier's products are not listed.
Quote:
... where DNA binding buffer was replaced with Oligo binding buffer (Zymo).
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No products found
because this supplier's products are not listed.
Yoshikazu Matsuoka, et al.,
bioRxiv - Cell Biology 2018
Quote:
... in Annexin V Binding Buffer (Biolegend). Then ...
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No products found
because this supplier's products are not listed.
Quote:
GFP-binding protein (GBP) (Rothbauer et al., 2008) was fused to the Fc domain of human IgG (pIg-Tail) (R&D Systems), tagged with His6 in pET28a (EMD Biosciences) ...
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No products found
because this supplier's products are not listed.
Quote:
Solution protein-protein binding was monitored by microscale thermophoresis (MST) with a Monolith NT.115 (Nanotemper). The target proteins were portions of the CsoS2 NTD fused to Superfolder GFP and used at a concentration of 50 nM ...
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No products found
because this supplier's products are not listed.
Bryan C Jensen, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... at 0.25 μg/ml or rabbit antibody against calmodulin binding peptide Calmodulin Binding Peptide (GenScript) at 0.1 μg/ml ...
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No products found
because this supplier's products are not listed.
Quote:
... we harvested culture supernatant and filtered it through an Acrodisc 0.45 µm low protein-binding filter (Pall Laboratory, Port Washington, NY). We underlaid 1 mL of filtered supernatant with 1 mL of 20% (w/v ...
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No products found
because this supplier's products are not listed.
Quote:
... and binding detected with streptavidin-alkaline phosphatase (Jackson Immunoresearch) at 1:1000 for 1 h ...
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No products found
because this supplier's products are not listed.
Quote:
... antibody binding was detected using fluorescent secondary antibodies (LI-COR Biosciences ...
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No products found
because this supplier's products are not listed.
Daria Sicari, et al.,
bioRxiv - Cell Biology 2020
Quote:
... PG13-luc (WT p53 binding sites) was a gift from Bert Vogelstein (Addgene plasmid # 16442 ...
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No products found
because this supplier's products are not listed.
Graham A. Turner, et al.,
bioRxiv - Physiology 2018
Quote:
... Non-specific binding was blocked by application of normal horse serum (PK-6102; Vector Laboratories) diluted in tris-buffered saline ...
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No products found
because this supplier's products are not listed.
Emma J Kelsall, et al.,
bioRxiv - Molecular Biology 2019
Quote:
... Protein samples were run on a SDS-PAGE gel and proteins were visualised by InstantBlue protein stain (Expedeon). Quantitation of the intensities of Ras1.G17V (1-172 ...
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No products found
because this supplier's products are not listed.
Peter Mayrhofer, et al.,
bioRxiv - Bioengineering 2018
Quote:
... coli containing N-RBPS-LUCIF-F01 prepared by Eurogentec was thawed and re-suspended in 50 mM TRIS – HCl buffer pH 8.0 containing 10 mM EDTA ...
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No products found
because this supplier's products are not listed.
Lucía García-Gutiérrez, et al.,
bioRxiv - Cancer Biology 2018
Quote:
... Annexin V binding was assayed in MACSQuant VYB (Miltenyi Biotec) and the results were analyzed with Flow Logic software (Miltenyi Biotec).
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No products found
because this supplier's products are not listed.
Justin Parreno, et al.,
bioRxiv - Cell Biology 2019
Quote:
... Total protein of samples loaded into capillaries was determined using a Total Protein Separation module kit (Protein Simple). Protein expression of specific molecules were normalized to total protein.
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No products found
because this supplier's products are not listed.
Quote:
... and of a FAM-labeled PNA probe (ATTCGTTGGAAACGGGA) that is directed against the CENP-B binding site in the centromeric alpha satellite DNA (CENP-B-FAM, PNA Bio) was added to the TMA ...
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No products found
because this supplier's products are not listed.
Quote:
... The chromosomal binding proteins were then separated using BioRuptor waterbath sonicator (Diagenode) at low setting for 5 minutes ...
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No products found
because this supplier's products are not listed.
Laura E. Schultz-Rogers, et al.,
bioRxiv - Genetics 2018
Quote:
... Membranes were incubated in either rabbit polyclonal anti-RB Binding Protein 4 RBBP4 (Bethyl A301-206A-T) at 1:800 or mouse monoclonal anti-β-actin (Sigma A2228 ...
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No products found
because this supplier's products are not listed.
C. Garrett Rappazzo, et al.,
bioRxiv - Immunology 2020
Quote:
... and ADI-56046) were subject to two rounds of selection for binding to a recombinant SARS-CoV-2 S1 protein (Sino Biological, Cat # 40591-V08H). Induced yeast libraries covering at least 10-fold of their respective diversities were incubated with 10 or 1 nM biotinylated SARS-CoV-2 S1 protein under equilibrium conditions ...
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No products found
because this supplier's products are not listed.
Pan Liu, et al.,
bioRxiv - Biochemistry 2020
Quote:
... followed by concentrating and buffer exchange into IMAC binding buffer using Vivaflow 100 system (Sartorius). The resulting protein solution was purified by Histrap column (GE Healthcare ...
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No products found
because this supplier's products are not listed.
Edward D. Kerr, et al.,
bioRxiv - Biochemistry 2020
Quote:
Proteins and peptides were identified with Protein Pilot 5.1 (SCIEX) as previously described1,16 ...
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No products found
because this supplier's products are not listed.
David Easterhoff, et al.,
bioRxiv - Immunology 2019
Quote:
... Binding was detected with an anti-IgG-HRP (Rockland) and developed with SureBlue Reserve TMB One Component (KPL) ...
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No products found
because this supplier's products are not listed.
Quote:
... Total protein was extracted using PEB protein extraction buffer (Agrisera Antibodies) according to the manufacturer‘s instructions ...
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No products found
because this supplier's products are not listed.
Quote:
... 0.325 μg/μl Maltose Binding Protein (MBP)-AviTag substrate (Avidity, L.L.C.), 8.3 mM ATP and 42 μM biotin ...
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No products found
because this supplier's products are not listed.
Quote:
... Antibody binding was detected using anti-mouseHRP (1:5000, Proteintech, SA00001-1) or anti-rabbitHRP (1:2500 ...
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No products found
because this supplier's products are not listed.
Quote:
... Membranes were blocked to reduce nonspecific binding using 5% milk with PBS-TWEEN® tablets (PBS-T) (Calbiochem, USA) at room temperature (RT ...
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No products found
because this supplier's products are not listed.
Quote:
... Binding reactions for super shift assays were performed for 30 min at 4-8°C using the Binding Buffer B-1 (Active Motif 37480) together with Stabilizing Solution D (Active Motif 37488 ...
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No products found
because this supplier's products are not listed.
Quote:
... Dengue virus NS4B protein (Genetex, GTX124250) at 1:1000 ...
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No products found
because this supplier's products are not listed.
Juha Auvinen, et al.,
bioRxiv - Epidemiology 2018
Quote:
... and at 5-weeks of age the mice were transferred to Teklad Global 19% Protein Extruded Rodent Diet (19.0% protein, 9.0% fat, 44.9% carbohydrates; Envigo) to slightly support the increase in adipocity upon aging ...
Catalog #: P2010013 - 0.5 mg Retinol Binding Protein (RBP) is a carrier protein with mutiple...Cat# P2010013, USD $395.00/ea Ask