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No products found
because this supplier's products are not listed.
Quote:
... EVs were incubated with Gal-3 (Gal-3 human recombinant, expressed in E. coli, Sigma-Aldrich) at different final concentration (0 to 10 µg/ml) ...
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No products found
because this supplier's products are not listed.
Donatas Repecka, et al.,
bioRxiv - Synthetic Biology 2019
Quote:
... The soluble recombinant MDH mutants were purified by using HisPur™ Ni-NTA Spin columns (ThermoFisher Scientific, USA). The columns with loaded supernatants were washed with Wash buffer (0.1 M Potassium phosphate buffer ...
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No products found
because this supplier's products are not listed.
Yanli Liu, et al.,
bioRxiv - Biochemistry 2019
Quote:
... The recombinant proteins were overexpressed in E coli BL21 (DE3) Codon plus RIL (Stratagene) at 15 °C and purified by affinity chromatography on Ni-nitrilotriacetate resin (Qiagen ...
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No products found
because this supplier's products are not listed.
Shutao He, et al.,
bioRxiv - Plant Biology 2020
Quote:
... and λ protein phosphatase (λ-Pase from E. coli; New England Biolabs, Beverly, MA). DDT or NADP treatments were performed as described by Liu et al ...
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No products found
because this supplier's products are not listed.
Quote:
... and 1µg of recombinant human E-cadherin (R&D systems). Coverslips were then washed two times with PBS.
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No products found
because this supplier's products are not listed.
Quote:
... Recombinant proteins (GAPDH, Abcam; Vimentin ...
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No products found
because this supplier's products are not listed.
Quote:
... coli BL21 (DE3) strain for protein production (GE Healthcare™). After the required plasmid was introduced into BL21(DE3) ...
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No products found
because this supplier's products are not listed.
Quote:
... Purified recombinant luciferase protein (Promega) was used to generate a standard curve.
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No products found
because this supplier's products are not listed.
Quote:
... coli protein production (Genscript) and used as templates for subsequent cloning ...
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No products found
because this supplier's products are not listed.
Luke A. Perera, et al.,
bioRxiv - Biochemistry 2019
Quote:
Mutant Chinese hamster BiP proteins with an N-terminal His6-tag were purified as described before with modifications (Preissler et al, 2017b). Proteins were expressed in M15 Escherichia coli (E. coli) cells (Qiagen). The bacterial cultures were grown in LB medium supplemented with 100 µg/ml ampicillin and 50 µg/ml kanamycin at 37 ° C to an OD600nm of 0.8 and expression was induced with 1 mM IPTG ...
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No products found
because this supplier's products are not listed.
Takumi Takeuchi, Mami Hattori-Kato,
bioRxiv - Genomics 2017
Quote:
... Competent cells (E. coli HST08, TaKaRa, Kusatsu, Japan) were transformed with those ligation products and vectors with variations in the mouse kidney AE1 promoter region were obtained and validated with Sanger sequencing using RV3 and luc2St-R primers ...
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No products found
because this supplier's products are not listed.
Quote:
... we used Escherichia coli k-12 strain MG1655 (hereafter E. coli; Roche, Basel, Switzerland), which has a high-quality genome sequence available (GenBank accession NC_000913 ...
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No products found
because this supplier's products are not listed.
Sara R van Boeckel, et al.,
bioRxiv - Immunology 2017
Quote:
... 1 μg/ml E-coli DNA (dsDNA E-coli Invivogen), or 0.5 μg/ml cff-DNA ...
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No products found
because this supplier's products are not listed.
Quote:
... Recombinant proteins (GAPDH, Abcam; Vimentin, PeproTech; Bid ...
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No products found
because this supplier's products are not listed.
Jennifer Y. Tan, et al.,
bioRxiv - Genomics 2018
Quote:
... 0.01% recombinant mouse LIF protein (Merck, #ESG1107) and 0.06 mM 2-Mercaptoethanol (Thermo Fisher ...
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No products found
because this supplier's products are not listed.
Quote:
... coli (Epicentre), in pJET1.2 using the Thermo Scientific CloneJET PCR Cloning Kit (Thermo Scientific).
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No products found
because this supplier's products are not listed.
Quote:
... Recombinant Cas9 protein (PNA Bio) and a synthetic sgRNA (Synthego ...
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No products found
because this supplier's products are not listed.
Quote:
... Lyophilized Escherichia coli whole protein extract (Bio-Rad) was dissolved in 200 μL 0.1 M TEAB ...
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No products found
because this supplier's products are not listed.
Ziyin Wu, et al.,
bioRxiv - Neuroscience 2018
Quote:
... and recombinant human BDNF protein (R&D Systems, cat. no. 248-BD-025).
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No products found
because this supplier's products are not listed.
Xiaojing Chi, et al.,
bioRxiv - Microbiology 2020
Quote:
... Recombinant proteins were purchased from Sino Biological (Beijing, China) for SARS-CoV-2 RBD (40592-V05H ...
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No products found
because this supplier's products are not listed.
Xueping Liu, et al.,
bioRxiv - Genetics 2018
Quote:
... Recombinant human HIC1 purified protein (ORIGENE #TP322752) was obtained from ORIGENE (expressed in HEK293 using TrueORF clone ...
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No products found
because this supplier's products are not listed.
Quote:
... and transformed to DH5α competent cells (Z-Competent E. Coli Cells—Strain Zymo 5α, ZYMO Research, USA) for screening successful insertions (blue-white selection ...
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No products found
because this supplier's products are not listed.
Jasper Che-Yung Chien, et al.,
bioRxiv - Genetics 2020
Quote:
Cas9 recombinant protein was expressed in Escherichia coli BL21 (DE3) from plasmid pMJ915 (a gift from Jennifer Doudna; Addgene # 69090) and purified as previously described (34) ...
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No products found
because this supplier's products are not listed.
Quote:
... Images of each sample were taken on a fluorescence microscope (Nikon ECLIPSE E600 for E. coli and Nikon ECLIPSE Ti2-E for B ...
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No products found
because this supplier's products are not listed.
Quote:
... coli Sigma70 (BioLegend, USA distributed via Brunschwig ...
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No products found
because this supplier's products are not listed.
Quote:
... E-Cadherin (Cell Signaling), HSP90 (Cell Signaling) ...
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No products found
because this supplier's products are not listed.
Quote:
... GR (Santa Cruz E-20), and H3K27ac (Active Motif 39133) ...
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No products found
because this supplier's products are not listed.
Chunhua Shi, et al.,
bioRxiv - Pathology 2020
Quote:
... Lipopolysaccharide (LPS; from E. coli O111:B4, Cedarlane;) the principal component of Gram-negative bacteria that activates the innate immune system via TLR4 ...
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No products found
because this supplier's products are not listed.
Quote:
... E coli polar lipids (Avanti Polar Lipids, Alabaster, AL) in chloroform were dried under argon in a round-bottomed flask ...
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No products found
because this supplier's products are not listed.
Yi-Hua Jan, et al.,
bioRxiv - Cancer Biology 2017
Quote:
... and E-cadherin protein expressions: AK4 (Genetex, 1:200), HIF-1α (Cell signaling ...
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No products found
because this supplier's products are not listed.
Quote:
... coli constitutively expressing the Green Fluorescent protein present on biofilms was examined by laser scanning confocal microscopy (Leica TCS SP8) using oil immersion at 40X magnification ...
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No products found
because this supplier's products are not listed.
Sue Yu, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... Platinum-E cells (Plat-E) (RV-101, Cell Biolabs. San Diego, CA) and Oct4-GFP mouse embryonic fibroblasts (MEFs ...
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No products found
because this supplier's products are not listed.
Quote:
... Protein overexpression was performed using Escherichia coli BL21 (DE3) pLysS cells (BIOLINE) in super broth supplemented with 200 µg/mL ampicillin (AMRESCO ...
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No products found
because this supplier's products are not listed.
Quote:
... transferred into lysing matrix E tubes (MP Biomedicals), frozen in liquid nitrogen and stored at -80 °C ...
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No products found
because this supplier's products are not listed.
Quote:
... Recombinant proteins were dialyzed in a Slide-a-lyzer (VWR, 514-0172) overnight in ATPase buffer (100mM potassium acetate ...
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No products found
because this supplier's products are not listed.
Melanie Maya Kaelberer, et al.,
bioRxiv - Genetics 2019
Quote:
Mice received three intranasal administrations with either lipopolysaccharide (E. Coli, 0111:B4 Calbiochem) or vehicle (saline) ...
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No products found
because this supplier's products are not listed.
Quote:
... E-and M-proteins using the ProteinPilot 4.5 software (July 2012, AB SCIEX) that included variable modification (size-226.0776 Da ...
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No products found
because this supplier's products are not listed.
Sujit Pujhari, et al.,
bioRxiv - Microbiology 2017
Quote:
... 105 PFU of ZIKV was incubated with 1000 ng recombinant human Hsp70 (rhHsp70) protein (Enzo Life Science) or BSA (control ...
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No products found
because this supplier's products are not listed.
Quote:
... purified recombinant EGFP (Biovision, Milpitas, California) was diluted to a final concentration of 20 mM in PBS (Gibco ...
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No products found
because this supplier's products are not listed.
Jiyeon Choi, et al.,
bioRxiv - Genomics 2019
Quote:
... or 100-750ng YY1 full-length recombinant protein (31332, Active Motif) in binding buffer containing 10 mM Tris (pH 7.5) ...
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No products found
because this supplier's products are not listed.
Quote:
... recombinant proteins were immobilized on a 96-well flat bottom plate (Corning, CLS3595) by incubation at 4 °C for 12-16 hr ...
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No products found
because this supplier's products are not listed.
Bianca Castro Gouveia-Mageste, et al.,
bioRxiv - Plant Biology 2020
Quote:
... Total protein extracts were obtained from infiltrated leaves expressing the recombinant proteins and incubated for 2 h with anti-green fluorescent protein (GFP) magnetic beads (MACS / Miltenyi Biotec) at 4 °C under rotation ...
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No products found
because this supplier's products are not listed.
Quote:
Recombinant full length human PFKFB3 protein purified from Sf9 baculoviral system acquired from SignalChem (Cat. #P323-30G). ATP ...
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No products found
because this supplier's products are not listed.
Xue Bessie Su, et al.,
bioRxiv - Cell Biology 2019
Quote:
... Fractions containing the eluted protein were run on an SDS-PAGE gel and the purity of recombinant Sgo1 was assessed by InstantBlue (Expedeon) staining.
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No products found
because this supplier's products are not listed.
Quote:
... UBE1 (50 nM, Boston Biochem, E-305), UBE2D1 (400nM Boston Bichem ...
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No products found
because this supplier's products are not listed.
Sarah E. Hill, Daniel Colón-Ramos,
bioRxiv - Cell Biology 2018
Quote:
... oil objective on a NikonTi-E stand (PerkinElmer) with a Hammamatsu C9100-50 camera ...
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No products found
because this supplier's products are not listed.
Quote:
... S1B,E and an Odyssey CLx imaging scanner (LI-COR) for Figure 6 ...
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No products found
because this supplier's products are not listed.
Jorge El-Azaz, et al.,
bioRxiv - Plant Biology 2019
Quote:
... Poly-His tagged recombinant proteins were purified using a nickel resin (Protino Ni-TED 2000 Packed Columns, Macherey-Nagel) and exchanged to Tris buffer 50 mM pH 8.0 in Sephadex™ G-25 M resin (PD-10 Columns ...
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No products found
because this supplier's products are not listed.
Quote:
... Escherichia coli was grown aerobically in LB medium (Teknova) at 37°C (58) ...
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No products found
because this supplier's products are not listed.
Quote:
... coli DH10B competent cells were transformed using a multiporator (Eppendorf, Germany). The target insert of the mamK gene was sequenced using the primer М13F ...