-
No products found
because this supplier's products are not listed.
Quote:
... individual colonies were picked and plated into E8 media (ThermoFisher). Clones were further expanded and characterized using standard assays for pluripotency ...
-
No products found
because this supplier's products are not listed.
Johnvesly Basappa, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... Colony formation assay was performed with MethoCult methylcellulose-based media as per manufacturer’s protocol (Stemcell Technologies, Vancouver, British Columbia, Canada). After 14 days ...
-
No products found
because this supplier's products are not listed.
Quote:
Colony forming assay was carried out using Noble agar (Sigma-Aldrich #A5431). For the lower layer ...
-
No products found
because this supplier's products are not listed.
Quote:
... Colony formation assay in Matrigel (BD Biosciences, Franklin Lakes, NJ) was performed as described previously [30].
-
No products found
because this supplier's products are not listed.
Kathryn L. Wofford, et al.,
bioRxiv - Bioengineering 2019
Quote:
... All conditioned media was supplemented with 20 ng/mL of macrophage colony stimulating factor (MCSF; Peprotech, cat. #300-25) to induce monocyte to macrophage differentiation ...
-
No products found
because this supplier's products are not listed.
Alex Kreymerman, et al.,
bioRxiv - Cell Biology 2018
Quote:
... media was exchanged with Assay Media (Agilent, 102365-100) and FluxPak injectable ports were loaded with drugs as recommended by mitochondrial stress test kit (Agilent ...
-
No products found
because this supplier's products are not listed.
Quote:
... We passaged these new iPSC colonies on MEF in hESC media (DMEM/F12 (Corning) supplemented with 20% KOSR (LifeTechnologies) ...
-
No products found
because this supplier's products are not listed.
John P. Marken, Richard M. Murray,
bioRxiv - Synthetic Biology 2019
Quote:
Cells were grown from a single colony overnight in 3 mL of M9CA minimal media (Teknova). Cells were diluted 1:100 into 300 uL fresh M9CA media without chloramphenicol in the morning in a 96-well square-well plate (Brooks MGB096-1-2-LG-L ...
-
No products found
because this supplier's products are not listed.
Quote:
... Plasmids were isolated from positive colonies (Qiagen) and confirmed by sequencing for the intended mutations.
-
No products found
because this supplier's products are not listed.
Caesar M Hernandez, et al.,
bioRxiv - Neuroscience 2019
Quote:
... male Fischer 344 x Brown Norway F1 hybrid (FBN) rats were obtained from the National Institute on Aging colony (Charles River Laboratories) and individually housed in the Association for Assessment and Accreditation of Laboratory Animal Care International-accredited vivarium facility in the McKnight Brain Institute building at the University of Florida in accordance with the rules and regulations of the University of Florida Institutional Animal Care and Use Committee and National Institutes of Health guidelines ...
-
No products found
because this supplier's products are not listed.
Quote:
The concentration of soluble HA in the media was quantified from sample media using an enzyme-linked immunosorbent assay (ELISA, R&D systems) following the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Quote:
... Colonies were subcultured in 1 mL mating selective media (SD-Leu-Trp, Clontech) and grown for two nights at 30oC with shaking ...
-
No products found
because this supplier's products are not listed.
Miharu Maeda, Yukie Komatsu, Kota Saito,
bioRxiv - Cell Biology 2019
Quote:
... HA (rat; Roche), and Sec31 (mouse ...
-
No products found
because this supplier's products are not listed.
Pooja Seedhar, et al.,
bioRxiv - Cell Biology 2016
Quote:
... BrdU (rat, Abcam), Cadm1 (chicken ...
-
No products found
because this supplier's products are not listed.
Davor Bajic, Kerry A. Chester, Dario Neri,
bioRxiv - Bioengineering 2019
Quote:
... rat anti-CD8 (Biolegend; 100702), rat anti-FoxP3 (eBioscience ...
-
No products found
because this supplier's products are not listed.
Hugo Girão, et al.,
bioRxiv - Cell Biology 2019
Quote:
... rat anti-RFP (ChromoTek) 1:1000 ...
-
No products found
because this supplier's products are not listed.
CheukMan C. Au, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... Ampicillin resistance colonies were selected for miniprep/maxiprep (Promega). For virus production ...
-
No products found
because this supplier's products are not listed.
Quote:
... 7 were bred in-house as wildtypes of a transgenic rat breeding colony and 23 additional animals were purchased from Envigo. The number of animals purchased was determined by litter sizes in order to achieve appropriate power ...
-
No products found
because this supplier's products are not listed.
Gabriela Gastélum, Mayra de la Torre, Jorge Rocha,
bioRxiv - Microbiology 2019
Quote:
... Colony area was measured using the Image Lab™ Software (BIORAD) and radial growth was calculated ...
-
No products found
because this supplier's products are not listed.
Quote:
... Colony PCRs were performed with Taq polymerase (NEB). PCR products and digested plasmids were purified with the Monarch PCR & DNA Cleanup Kit (NEB) ...
-
No products found
because this supplier's products are not listed.
Gregory L. Sousa, et al.,
bioRxiv - Microbiology 2020
Quote:
... and anti-rat (Jackson ImmunoResearch) secondary antibodies ...
-
No products found
because this supplier's products are not listed.
Angel K. Kongsomboonvech, et al.,
bioRxiv - Immunology 2020
Quote:
... and Il12b-/- (colony 002693) and wildtype C57BL/6J (B6) (colony 000664) mice were purchased from Jackson Laboratories, and all of the C57BL/6 background ...
-
No products found
because this supplier's products are not listed.
Quote:
... Colonies were visualized by staining with Giemsa (Merck) at 3 weeks post-electroporation.
-
No products found
because this supplier's products are not listed.
Quote:
... media was replaced with Ultraculture media (Lonza) supplemented with 100 U/mL Penicillin+100 µg/mLStreptomycin (Corning) ...
-
No products found
because this supplier's products are not listed.
J. Secklehner, et al.,
bioRxiv - Immunology 2019
Quote:
... Rat-IgG (2A3, BioXcell) and Hamster-IgG (Life Technologies ...
-
No products found
because this supplier's products are not listed.
Quote:
... Cell colonies were selected by 1mg/ml G418 antibiotic (Santa Cruz Biotechnologies). RNA interference against MUS81 was performed as previously reported (11) ...
-
No products found
because this supplier's products are not listed.
Quote:
... colonies were imaged using SZX-16 stereo microscope (Olympus) wherein the light source was above the colony ...
-
No products found
because this supplier's products are not listed.
Quote:
... Colonies were evaluated by colony PCR followed by plasmid isolation from overnight culture using peqGOLD Plasmid Miniprep Kit I (VWR). All sequences were confirmed by Sanger sequencing (Sequencing Core Facility ...
-
No products found
because this supplier's products are not listed.
Quote:
... Mating was performed on 1536-colony format YPD plates using a RoToR bench top colony arrayer (Singer Instruments; Somerset, UK). Resulting diploids were selected for deletion/DAmP libraries and ER-GFP markers KanR and URA3 ...
-
No products found
because this supplier's products are not listed.
Quote:
... Colonies were transferred on a cellophane membrane (GE Healthcare, UK) to primary and secondary ½ CD selection medium with 150 mg/L timentin (Duchefa ...
-
No products found
because this supplier's products are not listed.
Quote:
... and rat (Illumina). RNAs longer than 200 nt were selectively recovered using the RNA Clean & Concentrator-5 kit (Zymo Research) ...
-
No products found
because this supplier's products are not listed.
Quote:
... Cyanobacterial colonies were picked up under a dissecting microscope (Leica, Leica Microsystems, Wetzler, Germany) with pulled capillary pipettes and forceps to isolate colonies ...
-
No products found
because this supplier's products are not listed.
Quote:
... Transformed colonies were screened for the respective insert using the Colony Fast-Screen Kit (Epicentre; Madison, Wisconsin, USA) following the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
MG Lloyd, JL Vossler, CT Nomura, JF Moffat,
bioRxiv - Microbiology 2018
Quote:
... and colony diameter was measured with Living Image software (Perkin Elmer). Biofilms were stained with 0.1% crystal violet ...
-
No products found
because this supplier's products are not listed.
Quote:
... and anti-Rat biotinylated (1:100, Vector Labs) followed by peroxidase-conjugated streptavidin ...
-
No products found
because this supplier's products are not listed.
Tao zhang, et al.,
bioRxiv - Genomics 2016
Quote:
... 10 independent colonies were chosen for sequencing detection using the T7F primer by ABI 3130 automated sequencer (BeijingAuGCTCo.,Ltd) ...
-
No products found
because this supplier's products are not listed.
Quote:
... and rat anti-FLAG (1:300, Novus Biologicals). The following secondary antibodies were obtained from Jackson ImmunoResearch ...
-
No products found
because this supplier's products are not listed.
Yao Xiao, et al.,
bioRxiv - Physiology 2020
Quote:
... We collected media to assess lactose using a commercial assay kit (BioVision, Milpitas, CA). Lactose was corrected for tissue mass.
-
No products found
because this supplier's products are not listed.
Dinesh Kumar, et al.,
bioRxiv - Cancer Biology 2019
Quote:
... Cell colonies were photographed by digital camera (Nikon) and were counted using ImageJ software ...
-
No products found
because this supplier's products are not listed.
Hui San Chin, et al.,
bioRxiv - Cell Biology 2018
Quote:
... and anti-rat IgG (Southern Biotech).
-
No products found
because this supplier's products are not listed.
Quote:
... media was replaced with 10 mL basic heart media supplemented with 2 μM Wnt-C59 (Tocris #5148). On day 5 (48 hours later) ...
-
No products found
because this supplier's products are not listed.
Quote:
... All media efflux assay measurements were collected with the SpectraMax M2 (Molecular Devices, Sunnyvale CA) microtiter plate reader ...
-
No products found
because this supplier's products are not listed.
Quote:
... media was replaced with fresh media containing 40 μg/ml cycloheximide (Cell Signaling Technology). Cells were lysed at various time points after cycloheximide treatment in RIPA buffer ...
-
No products found
because this supplier's products are not listed.
Quote:
... Six colonies per genotype were added to CSM-Trp media (Sunrise Science Products) in a 96-well plate (Corning CLS3788 ...
-
No products found
because this supplier's products are not listed.
Annie Lee, et al.,
bioRxiv - Neuroscience 2019
Quote:
... 10% mouse astrocyte conditioned media (ACM; ScienCell) was also added to the medium ...
-
No products found
because this supplier's products are not listed.
Quote:
... Successfully transfected colonies were selected by plating on 0.5 μg/ml puromycin (InvivoGen). All plasmids were sequenced before use.
-
No products found
because this supplier's products are not listed.
Quote:
... media was replaced with Fluorobright fluorescent imaging media containing 500 nM sirDNA (Cytoskeleton). The chamber was then transferred to a Nikon Ti microscope fitted with an OKOlab environmental control chamber ...
-
No products found
because this supplier's products are not listed.
Quote:
... Stocks of peptide analogs of ACP and AKH were used for preparation of serial dilutions all of which were prepared in assay media (0.1% BSA in DMEM:F12) and loaded in quadruplicates into 96-well white luminescence plates (Greiner Bio-One, Germany). Cells were loaded with an automatic injector into each well of the plate containing different peptide analogs at various concentrations as well as negative control (assay media alone ...
-
Protein Kinase A ( PKA ) Activity Assay ELISA / assay Kit
Cat# K027-H1,
1x96 well plate, USD $430.0/ea
Ask
Quote:
... primary neuronal culture media or freshly dissected tissue from the POA were stored at -80° C for PGE2 (Arbor Assays, Cat# K051) and PGD2 (Cayman Chemicals ...
-
No products found
because this supplier's products are not listed.
Peichuan Zhang, et al.,
bioRxiv - Genetics 2019
Quote:
The assay was performed with the HT Fluorescent Homogeneous PARP Inhibition Assay Kit (Trevigen), following the manufacturer’s instructions ...