-
No products found
because this supplier's products are not listed.
Quote:
... Supernatants and lysates were separated by SDS-PAGE followed by immunoblotting with rat anti-HA (Sigma) antibody to evaluate the presence of recombinant CD47nb protein in culture fractions ...
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No products found
because this supplier's products are not listed.
Quote:
... Cell lysates and Protein A Dynabeads (Invitrogen) complexed with Ab-panAGO-2A8 (MABE56 Millipore ...
-
No products found
because this supplier's products are not listed.
Quote:
... cell lysates were harvested using RIPA buffer (Cell Signaling Technologies) with protease inhibitors (cOmplete ...
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No products found
because this supplier's products are not listed.
Quote:
Viral RNA was extracted from clarified cell lysates or samples obtained from cotton rats as described [55] by using the Mini Viral RNA Kit (Qiagen Sciences, Germantown, Maryland) and automated platform QIAcube (Qiagen ...
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No products found
because this supplier's products are not listed.
Quote:
... Whole cell lysates were loaded on 10% polyacrylamide gels (Biorad). Western blots were performed as previously described [22] ...
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No products found
because this supplier's products are not listed.
Christoph B. Messner, et al.,
bioRxiv - Systems Biology 2020
Quote:
... 5 µg of K562 cell lysate (Promega) or 5 µg yeast digests were injected and run on a nanoAcquity UPLC (Waters ...
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No products found
because this supplier's products are not listed.
Jonathan C Miller, et al.,
bioRxiv - Microbiology 2019
Quote:
... Cell lysate was bound to IgG-Sepharose beads (Roche) and washed by centrifugation with 100 mM NaCl ...
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No products found
because this supplier's products are not listed.
Quote:
... Cell lysate was applied to Strep-Trap HP column (GE Healthcare), prepared as suggested by the manufacturer ...
-
No products found
because this supplier's products are not listed.
Casey E. Vantucci, et al.,
bioRxiv - Bioengineering 2020
Quote:
... cells were blocked with purified anti-rat CD32 (BD) to prevent nonspecific binding ...
-
No products found
because this supplier's products are not listed.
Clement Gallay, et al.,
bioRxiv - Microbiology 2019
Quote:
... Cell lysate was incubated with equilibrated GFP-Trap resin (Chromotek) at 4°C for 2 h ...
-
No products found
because this supplier's products are not listed.
Quote:
... rat endothelial cell antigen-1 (RECA-1) (Mouse anti-rat, 1:100, Abcam, Cambridge, Massachussets, USA); p75(NTR ...
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No products found
because this supplier's products are not listed.
Quote:
... Cells were incubated with APC anti-rat (Biolegend) for anti-Liv2 ...
-
No products found
because this supplier's products are not listed.
Xue Wang, et al.,
bioRxiv - Developmental Biology 2019
Quote:
... The viable sorted cell was mixed with purified rat UGM cells (250,000 cells per graft) in rat tail Collage Type I (4.42mg/ml, Corning, 354236). Next ...
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No products found
because this supplier's products are not listed.
Quote:
... The cell lysates were centrifuged and cleared with A/G beads (Santa Cruz Biotechnology ...
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No products found
because this supplier's products are not listed.
Danielle M. Diniz, et al.,
bioRxiv - Animal Behavior and Cognition 2020
Quote:
... we used SERT−/− rats and wild-type WistarCrI:WI rats (WT rats) from Charles River (Horst, the Netherlands) as behavioral wild-type controls (see experimental design in figure 1) ...
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No products found
because this supplier's products are not listed.
Rahima Ziane, Alain Camasses, Marta Radman-Livaja,
bioRxiv - Molecular Biology 2019
Quote:
... Zirconium beads were removed from the cell lysate by centrifugation and the entire cell lysate was subject to sonication using the Bioruptor-Pico (Diagenode) for 3×10 cycles of 30 seconds ON/OFF each resulting in a final median size of chromatin fragments of 200 bp ...
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No products found
because this supplier's products are not listed.
Quote:
... The cell free lysate was clarified by centrifugation and 100 μg of lysate was incubated with 100 µM Nitrocefin (484400-MERCK). Hydrolysis of nitrocefin was monitored at 486 nm emission (excitation at 390 nm ...
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No products found
because this supplier's products are not listed.
Miriam R. Fein, et al.,
bioRxiv - Cancer Biology 2018
Quote:
... or control rat IgG2b antibody (Bio X Cell) (Winograd et al. ...
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No products found
because this supplier's products are not listed.
Quote:
Single cell lysate was reverse transcribed using Smartscribe Reverse Transcriptase (Clontech) in a 10 ul reaction including a Smartseq2 33 TSO (Supplementary Table S1 ...
-
No products found
because this supplier's products are not listed.
Gulzhan Raiymbek, et al.,
bioRxiv - Biochemistry 2019
Quote:
... The cleared cell lysate was applied to Amylose resin (New England Biolabs) followed by washing with buffer A and elution with buffer A containing 10 mM maltose ...
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No products found
because this supplier's products are not listed.
Quote:
... The cell pellet was resuspended in 25 μL of 20% Normal Rat Serum (Jackson Immunoresearch) in PBS/1%BSA and incubated for 20 minutes on ice ...
-
No products found
because this supplier's products are not listed.
Jiuwei Lu, et al.,
bioRxiv - Biochemistry 2019
Quote:
... Cell lysate was centrifuged with a JA25.5 rotor (Beckman Coulter) at 39,000 g for 40min at 4 °C ...
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No products found
because this supplier's products are not listed.
Mark A. DeWitt, et al.,
bioRxiv - Cell Biology 2016
Quote:
... Cell lysates were characterized by HPLC (Infinity 1260, Agilent) using a weak cation-exchange column (PolyCAT A™ ...
-
No products found
because this supplier's products are not listed.
Tshegofatso Ngwaga, et al.,
bioRxiv - Microbiology 2018
Quote:
... or normal rat IgG control (Rat IgG; R&D Systems) were used at a concentration of 50ng mL-1.
-
No products found
because this supplier's products are not listed.
Quote:
... All rats had unlimited access to rodent chow (Teklad LM-485 Mouse/Rat 7012, Envigo) and water in the home cage and were individually housed in a temperature- and humidity-controlled vivarium maintained on a 12-h light-dark cycle (lights on from 0600 to 1800) ...
-
No products found
because this supplier's products are not listed.
Sierra Fox, et al.,
bioRxiv - Genetics 2019
Quote:
... Cell lysate was resuspended in transposition reaction mix (Illumina) and incubated at 37°C for 30 min ...
-
No products found
because this supplier's products are not listed.
Kyung-Jin Jang, et al.,
bioRxiv - Pharmacology and Toxicology 2019
Quote:
... human and rat stellate cells were purchased from Lonza, and each were cultured according to their respective vendor protocols ...
-
No products found
because this supplier's products are not listed.
Amalia Sintou, et al.,
bioRxiv - Immunology 2019
Quote:
... UK) were coated with 50μl per well of 4μg/μl pig heart lysate lysate (Novus Biologicals, Bio-Techne, Abingdon, UK) diluted in PBS overnight at 4°C ...
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No products found
because this supplier's products are not listed.
Quote:
... Cell lysates were prepared using the EasyLyseTM bacterial protein extract solution (Lucigen Corp. USA) or the CelLytic B reagent (Sigma ...
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No products found
because this supplier's products are not listed.
Quote:
... Total cell lysates were incubated with 1 μg of ASCC3 antibody (Bethyl laboratories, A304-015A) for 1 hr at 4°C on a rotator ...
-
No products found
because this supplier's products are not listed.
Quote:
... cell lysate (5 μl/well) was transferred to a 384-well ProxiPlate (PerkinElmer) and added to the donor and acceptor reaction mix (2.5 μl/ well ...
-
No products found
because this supplier's products are not listed.
Sarah Guimbal, et al.,
bioRxiv - Physiology 2020
Quote:
... ECs cells were labelled with rat anti-mouse CD31 microbeads (Miltenyi Biotec). Labelled ECs and attached cells were then isolated magnetically ...
-
No products found
because this supplier's products are not listed.
Mandy Boontanrart, et al.,
bioRxiv - Genetics 2020
Quote:
... Cell lysate concentrations were measured using Bradford reagent (VWR) or BCA assay (ThermoFisher) ...
-
No products found
because this supplier's products are not listed.
Emanuel Salazar-Cavazos, et al.,
bioRxiv - Systems Biology 2019
Quote:
... Cell lysates were collected using cell scrapers (Greiner Bio-One North America, #541070), transferred to fresh tubes on ice and vortexed every 5 min for a total of 20 min ...
-
No products found
because this supplier's products are not listed.
Quote:
... and anti-Rat biotinylated (1:100, Vector Labs) followed by peroxidase-conjugated streptavidin ...
-
No products found
because this supplier's products are not listed.
Sung-Jin Yoon, et al.,
bioRxiv - Microbiology 2018
Quote:
... cell lysates were incubated with glutathione MagBeads (GenScript) for 1 h at room temperature ...
-
No products found
because this supplier's products are not listed.
Paul R. Marshall, et al.,
bioRxiv - Neuroscience 2019
Quote:
... Lysate from cells or tissue was fixed in 1% formaldehyde and cross-linked cell lysates were sheared by Covaris in 1% SDS lysis buffer to generate chromatin fragments with an average length of 300bp ...
-
No products found
because this supplier's products are not listed.
Quote:
... Osteoblast-like MC3T3-E1 cells and rat mesenchymal stem cells (rMSC) (American Type Culture Collection, USA) were cultured in alpha-modified Eagle’s medium (Hyclone ...
-
No products found
because this supplier's products are not listed.
Kazuhito Honjo, et al.,
bioRxiv - Immunology 2019
Quote:
... Fisher rats (Jackson Laboratory) were immunized at 3 to 4 day intervals over a 3 week period and popliteal nodes were fused with the mouse plasmacytoma Ag8.653 and plated for selection in 96-well plates (Won et al. ...
-
No products found
because this supplier's products are not listed.
Quote:
Whole cell lysate or tissue lysate was extracted using RIPA lysis buffer (Boston Bioproducts) supplemented with complete protease inhibitor cocktail (Roche) ...
-
No products found
because this supplier's products are not listed.
Quote:
... HeLa cell lysate (human origin, Enzo Life Sciences, BML-SW8750, Switzerland) was incubated in the presence of 0.7 mM of biotinylated Fabs at 4 °C for 5 h ...
-
No products found
because this supplier's products are not listed.
Alex Hunt, et al.,
bioRxiv - Microbiology 2019
Quote:
... Goat anti-rat IRDye 680LT (LI-COR) (1:20000).
-
No products found
because this supplier's products are not listed.
L. S. Tran, et al.,
bioRxiv - Immunology 2019
Quote:
... lysate samples were probed with rat-anti-human Tubulin (0.9 ng/ml; Rockland, PA, USA), followed by goat-anti-rat secondary antibody-Alexa Fluor® 800 conjugate (0.33 ng/ml ...
-
No products found
because this supplier's products are not listed.
Quote:
... Cell lysates were immunoprecipitated using Universal Magnetic Co-IP Kit (Active Motif 54002), then analyzed by western blot ...
-
No products found
because this supplier's products are not listed.
Quote:
... Primed (EpiSC) N2B27 media for murine and rat cells (EpiSCs) contained 8ng/ml recombinant human bFGF (Peprotech Asia), 20ng/ml recombinant human Activin (Peprotech) ...
-
No products found
because this supplier's products are not listed.
Quote:
... Active RalB in cell lysates was measured via precipitation with RalBP1 PBD Agarose beads (Cell BioLabs, San Diego, CA). Lysates were prepared and precipitation was performed per manufacturer instructions ...
-
No products found
because this supplier's products are not listed.
Hui San Chin, et al.,
bioRxiv - Cell Biology 2018
Quote:
... and anti-rat IgG (Southern Biotech).
-
No products found
because this supplier's products are not listed.
Quote:
... Cell lysates were incubated overnight with 1 % ndodecyl β-D-maltoside (DDM, Calbiochem) at 4 °C ...
-
No products found
because this supplier's products are not listed.
Soraia Martins, et al.,
bioRxiv - Neuroscience 2019
Quote:
Total RNA was extracted from cell lysates using Direct-zol RNA Mini Prep kit (Zymo Research) in combination with peqGOLD TriFast (PeqLab Biotechnologie ...
-
No products found
because this supplier's products are not listed.
Pablo Hernansanz-Agustín, et al.,
bioRxiv - Biochemistry 2018
Quote:
... and siRNA against rat NCLX (SL8B1 gene) was purchased from OriGene (SLC8B1 Trilencer-27 Rat siRNA).