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Protein Digestion kit
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Axon Medchem Sponsored
![OSI 930 - CAS [728033-96-3], >99%](https://image.labshake.com/supplier-product/305cc4ba-a97e-4815-88b6-a95430b03ef4/45612fdf-fc85-4cf4-b930-a7e7f23bd699.png)
c-Kit and VEGFR2 tyrosine kinase inhibitor. Sold for research purposes only.Cat# 1547.0, SKU# 1547-25, 25 mg, US $352 / EA, EURO €320 / EA Ask
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Promega
No products found because this supplier's products are not listed.bioRxiv - Plant Biology 2018Quote: ... In-gel digestion of proteins with chymotrypsin (Promega) for 16 hr at 37° C followed by Trypsin (Promega ... -
Thermo Fisher
No products found because this supplier's products are not listed.bioRxiv - Genomics 2016, published in Molecular Cell doi: 10.1016/j.molcel.2017.01.023Quote: ... RNA was desiccated after protein digestion by Proteinase K (Ambion). RNA was reverse transcribed using barcoded oligo-dT primers (IDT ... -
Qiagen
No products found because this supplier's products are not listed.bioRxiv - Systems Biology 2019, published in npj Systems Biology and Applications doi: 10.1038/s41540-019-0121-4Quote: ... digestion and RNeasy MinElute Cleanup kit (QIAGEN) column purification ... -
Expedeon
No products found because this supplier's products are not listed.Cited in CSNAP, the smallest CSN subunit, modulates proteostasis through cullin-RING ubiquitin ligasesbioRxiv - Biochemistry 2018, published in Cell Death & Differentiation doi: 10.1038/s41418-019-0392-8Quote: ... using a FASP™ Protein Digestion Kit (Expedeon). The resulting peptides were desalted and analyzed on the LC-MS instrument (Q-Exactive Plus ... -
New England Biolabs
No products found because this supplier's products are not listed.bioRxiv - Molecular Biology 2018, published in Scientific Reports doi: 10.1038/s41598-018-30383-zQuote: ... After digestion by mung bean nuclease (MBN Kit: M0250S, NEB) and 0.05 mg/ml RNase A (Sigma-Aldrich ... -
Bio-Rad
No products found because this supplier's products are not listed.bioRxiv - Systems Biology 2018Quote: ... After determining protein concentrations (Bio-Rad Protein Assay Kit), immunoblotting was performed ... -
Millipore Sigma
No products found because this supplier's products are not listed.bioRxiv - Developmental Biology 2019Quote: ... in-gel tryptic digestion performed using proteoprofile trypsin in-gel digest kit (PP0100, Sigma-Aldrich). The samples were desalted using either ZipTip™ C18 (Millipore ... -
Roche
No products found because this supplier's products are not listed.bioRxiv - Systems Biology 2020Quote: ... and protein was removed by digestion with 1.2 mg/mL proteinase K (Roche) in proteinase K buffer (50 mM Tris-HCl at pH 7.5 ... -
Worthington Biochemical
A component of the Papain Dissociation System, for use in the tissue dissociation method of...Cat# LK003178, 5 vi, $88.0 AskbioRxiv - Molecular Biology 2019Quote: ... The enzyme digestion was carried out by digestion buffer (calcium-free Tyrode buffer supplemented with hyaluronidase [Worthington, 0.1 mg·ml-1 ... -
GE Life Sciences
No products found because this supplier's products are not listed.bioRxiv - Neuroscience 2018Quote: ... Protein quantification was done using a BCA protein assay kit (GE Healthcare). Appropriate volumes of 2X SDS sample buffer (10% glycerol ... -
Zymo Research
No products found because this supplier's products are not listed.bioRxiv - Genetics 2020Quote: ... We used ZR Fungal/Bacterial RNA mini-prep kit with DNase I digestion (Zymo Research R2014), following the kit manual ... -
Miltenyi Biotec
No products found because this supplier's products are not listed.bioRxiv - Immunology 2019Quote: ... enzymatic digestion (Tumor dissociation kit, human; Miltenyi Biotec) was performed on excised granulomas using gentleMACS octo dissociator ... -
Takara Bio
No products found because this supplier's products are not listed.bioRxiv - Zoology 2020Quote: ... The protein concentration was measured by TaKaRa BCA Protein Assay Kit (Takara Bio) in accordance with the manufacturer’s protocol ... -
Agilent
No products found because this supplier's products are not listed.bioRxiv - Physiology 2018, published in BioMed Research International doi: 10.1155/2019/7084734Quote: ... with proteinase K digestion (Dako, Glostrup, Denmark) used for antigen retrieval ... -
abcam
No products found because this supplier's products are not listed.bioRxiv - Molecular Biology 2018, published in EBioMedicine doi: 10.1016/j.ebiom.2018.11.016Quote: ... The total protein concentration was determined by using Bradford protein assay kit (Abcam) and the collagen content of each sample was normalized to total protein. -
Merck
No products found because this supplier's products are not listed.bioRxiv - Biochemistry 2018Quote: ... Protein carbonylation was assessed using the OxyBlot protein oxidation detection kit (Merck Millipore), and differential carbonylation was assessed by densitometry ... -
Macherey-Nagel GmbH
No products found because this supplier's products are not listed.bioRxiv - Zoology 2018, published in PLOS ONE doi: 10.1371/journal.pone.0209813Quote: ... and protein purification kit (Macherey-Nagel 740609.240C) following the manufacturers protocol ... -
Lonza
No products found because this supplier's products are not listed.bioRxiv - Cancer Biology 2020Quote: ... through digestion of mammary glands in 5 mL of digestion media (Lonza/Amaxa DMEM/F12 1:1 Mixture with HEPES ... -
Biovision
No products found because this supplier's products are not listed.bioRxiv - Molecular Biology 2019Quote: ... we extracted CY and PM proteins using the PM Protein Extraction Kit (BioVision). The protein samples were treated for 30 min at 50°C in a sample-treating solution containing 2% SDS and 5% β-mercaptoethanol ... -
Sartorius
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2019Quote: Protein samples were prepared using filter-aided sample preparation (FASP) digestion (Wisniewski et al., 2009) (Vicacon500, Sartorius, VN01H02 10kDA). The FASP filter were washed with 200 µl 0.1 % trifluoroacetic acid (TFA ... -
Eppendorf
No products found because this supplier's products are not listed.bioRxiv - Biochemistry 2019Quote: ... Enzymatic digestion proceeded for 1 hr at 37 °C using the ThermoMixer C (Eppendorf) shaking at 400 rpm ... -
Santa Cruz
No products found because this supplier's products are not listed.bioRxiv - Molecular Biology 2019, published in Nature Communications doi: 10.1038/s41467-020-14343-8Quote: ... Proteins were detected by immunoblotting with a chemiluminescence Luminol kit (SC-2048, Santa Cruz Biotechnology) using a LumiAnalyst Imager (Roche Applied Sciences). -
Biotek
No products found because this supplier's products are not listed.bioRxiv - Plant Biology 2019, published in Plant Communications doi: 10.1016/j.xplc.2020.100025Quote: ... Plant RNA kit with DNA digestion on column (Biotek, China) according to the manufacturer’s instructions ... -
Solarbio
No products found because this supplier's products are not listed.Cited in Airway epithelial integrin β4 suppresses allergic inflammation by decreasing CCL17 productionbioRxiv - Immunology 2019Quote: ... Protein concentration was measured by BCA protein determination kit (PC0020, Solarbio, China). Immunoprecipitations were performed overnight using the appropriate dilution of a polyclonal anti-ITGB4 antibody (Ab197772 ... -
Cytoskeleton
No products found because this supplier's products are not listed.bioRxiv - Genetics 2018, published in PLOS Genetics doi: 10.1371/journal.pgen.1007671Quote: ... recombinant proteins to a proprietary Ral effector protein was assessed using the RalA G-LISA™ Activation Assay Kit (#BK129, Cytoskeleton, Inc. Denver, CO), as per the manufacturer’s protocol ... -
R&D Systems
No products found because this supplier's products are not listed.bioRxiv - Cancer Biology 2017, published in Science Translational Medicine doi: 10.1126/scitranslmed.aar2718Quote: The effect of KHS101 on HSPD1 mediated protein folding was assessed using the human HSP60/HSP10 protein refolding kit (R&D Systems, Cat # K-300) following the manufacturer’s instructions (no unspecific effect of KHS101 on the luminescence signal of client protein in absence of heat-induced protein denaturation was observed). -
Bioline
No products found because this supplier's products are not listed.bioRxiv - Systems Biology 2017, published in Oncotarget doi: 10.18632/oncotarget.24047Quote: ... proteins were extracted using the ISOLATE II RNA/DNA/Protein Kit (BIOLINE). Protein concentration was determined using the MicroBCA Protein Assay Kit (Pierce-Thermo Scientific) ... -
Protein Simple
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2019Quote: ... Total protein of samples loaded into capillaries was determined using a Total Protein Separation module kit (Protein Simple). Protein expression of specific molecules were normalized to total protein. -
NanoTemper
No products found because this supplier's products are not listed.bioRxiv - Immunology 2019Quote: ... ILT7 purified protein was labeled using the Protein Labeling Kit RED-NHS (NanoTemper Technologies). The labeling reaction was performed according to the manufacturer’s instructions ... -
GenScript
No products found because this supplier's products are not listed.bioRxiv - Immunology 2020Quote: ... and the protein concentration was measured by the BCA protein assay kit (Genscript, Nanjing, China) as previous described25. -
TianGen Biotech
No products found because this supplier's products are not listed.bioRxiv - Biochemistry 2020Quote: ... Protein concentration was measured using Bradford protein assay kit (Tiangen Biotech, Beijing Co., LTD, CN). The purity of the PM was determined by standard marker assays[32] ... -
Becton, Dickinson and Company
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2018Quote: ... After dialysis and thrombin digestion (3 U/mg NA; BD Biosciences) overnight at 4°C ... -
Vector Labs
No products found because this supplier's products are not listed.bioRxiv - Developmental Biology 2018, published in Scientific Reports doi: 10.1038/s41598-019-40923-wQuote: ... the immunoreactive protein was visualized using the Vectastain ABC kit (Vector Laboratories) and 3,3’ diaminobenzidine tetrahydrochloride hydrate (Sigma ... -
PerkinElmer
No products found because this supplier's products are not listed.bioRxiv - Biochemistry 2020Quote: ... proteins were visualized using the Western Lightning ECL II Kit (Perkin Elmer) and exposed on X-Ray film (GE Healthcare Amersham Hyperfilm ECL ... -
Addgene
No products found because this supplier's products are not listed.bioRxiv - Developmental Biology 2018, published in Neuron doi: 10.1016/j.neuron.2019.07.005Quote: The p3E vectors were built by digestion of the p3E-MCS (Addgene #75174) with SalI/BamHI to clone a U6c:gRNA#1 sequence or by SpeI/SacII to clone U6d:gRNA#3 ... -
Calbiochem
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2018, published in Journal of Cell Science doi: 10.1242/jcs.221390Quote: ... The protein complexes bound to the beads were digested using ProteoExtract All-in-One Trypsin Digestion Kit (Cat. No. 650212, Calbiochem) according to manufacturer’s instructions ... -
VWR
No products found because this supplier's products are not listed.bioRxiv - Systems Biology 2020, published in Nature Communications doi: 10.1038/s41467-020-18164-7Quote: ... Protein and crosslink digestion was carried out by adding 50 μL freshly prepared 20 mg/mL Proteinase K (VWR, freeze-dried powder suspended in 10 mM Tris ... -
Cayman Chemical
No products found because this supplier's products are not listed.bioRxiv - Neuroscience 2019Quote: ... Protein content was determined using a Protein Determination Kit (Cayman Chemical, Cat# 704002) and absorbance was measured at 595 nm using a Nanodrop 2000 spectrophotometer (Thermofisher ... -
G-Biosciences
No products found because this supplier's products are not listed.bioRxiv - Biochemistry 2019Quote: ... The concentrations of these proteins were estimated by BCA protein estimation method (BCA assay kit, G-Biosciences). The in vitro ubiquitination assay was set up with the following components in the reaction mixture-5 nM Uba1p ... -
Cell Signaling Technology
No products found because this supplier's products are not listed.bioRxiv - Molecular Biology 2019Quote: ... 30 µL magnetic protein G beads (SimpleChIP Kit #9003, Cell Signaling Technology) were added to the samples and incubated at 4 °C with end over end rotation for 4 hours ... -
Active Motif
No products found because this supplier's products are not listed.bioRxiv - Pharmacology and Toxicology 2020Quote: Nuclear proteins were isolated from RAW264 cells using Nuclear Extract Kit (Active Motif, Carlsbad, CA, USA) according to the manufacturer’s protocols ... -
Sciex
No products found because this supplier's products are not listed.bioRxiv - Biochemistry 2020Quote: Proteins and peptides were identified with Protein Pilot 5.1 (SCIEX) as previously described1,16 ... -
PNA Bio
No products found because this supplier's products are not listed.bioRxiv - Immunology 2020Quote: ... Cas9 protein (PNA bio) and gRNA were incubated at room temperature for 15 min in a 3:1 reaction with 15μg Cas9 and 5000 ng gRNA (2500ng of sgRNA#1 and 2500ng of sgRNA#2).47 The incubation product (gRNA and Cas9 complex ... -
Illumina
No products found because this supplier's products are not listed.bioRxiv - Evolutionary Biology 2018, published in Genetics doi: 10.1534/genetics.118.300894Quote: ... The Pst I restriction enzyme was used for sequence digestion and DNA was sequenced on the Illumina HiSeq 2500 platform (Illumina Inc., USA). -
ChromoTek
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2019, published in Cell Reports doi: 10.1016/j.celrep.2020.01.033Quote: ... Immunoprecipitation was performed using crosslinked protein and a GFP-Trap®_A Kit (Chromotek) following the manufacturer’s instructions ... -
LI-COR
No products found because this supplier's products are not listed.Cited in Protective role of the deSUMOylating enzyme SENP3 in myocardial ischaemia-reperfusion injurybioRxiv - Cell Biology 2019Quote: ... Total protein on membranes was stained using REVERT total protein stain (LI-COR). Immune complexes were detected using HRP-conjugated secondary antibodies (Sigma ... -
BioLegend
No products found because this supplier's products are not listed.bioRxiv - Immunology 2019Quote: ... Type III IFN proteins were measured using a custom-designed LEGENDplex kit (BioLegend). IFN-β was measured using the Verikine ELISA (PBL) ... -
Corning
No products found because this supplier's products are not listed.Cited in Robust three-dimensional expansion of human adult alveolar stem cells and SARS-CoV-2 infectionbioRxiv - Cell Biology 2020Quote: ... followed by further dissociation using pre-warmed digestion buffer containing 2U/ mL Dispase II (Sigma, Corning), 1 mg/mL Dispase/Collagenase (Sigma ... -
Lucigen
No products found because this supplier's products are not listed.bioRxiv - Genomics 2019Quote: ... Non circularized templates were eliminated by 2 h digestion at 37°C with Plasmid-safe DNAse (Epicentre, #E3101K), the enzyme was inactivated by heating for 30 minutes at 70°C ... -
Agrisera
No products found because this supplier's products are not listed.bioRxiv - Plant Biology 2019, published in The Plant Journal doi: 10.1111/tpj.14716Quote: ... Total protein was extracted using PEB protein extraction buffer (Agrisera Antibodies) according to the manufacturer‘s instructions ...