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No products found
because this supplier's products are not listed.
Charles P. Moehs, et al.,
bioRxiv - Plant Biology 2018
Quote:
... In-gel digestion of proteins with chymotrypsin (Promega) for 16 hr at 37° C followed by Trypsin (Promega ...
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No products found
because this supplier's products are not listed.
Quote:
... RNA was desiccated after protein digestion by Proteinase K (Ambion). RNA was reverse transcribed using barcoded oligo-dT primers (IDT ...
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No products found
because this supplier's products are not listed.
Quote:
... using a FASP™ Protein Digestion Kit (Expedeon). The resulting peptides were desalted and analyzed on the LC-MS instrument (Q-Exactive Plus ...
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No products found
because this supplier's products are not listed.
Quote:
... digestion and RNeasy MinElute Cleanup kit (QIAGEN) column purification ...
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No products found
because this supplier's products are not listed.
Quote:
... After digestion by mung bean nuclease (MBN Kit: M0250S, NEB) and 0.05 mg/ml RNase A (Sigma-Aldrich ...
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No products found
because this supplier's products are not listed.
Chih-Chien Sung, et al.,
bioRxiv - Systems Biology 2018
Quote:
... After determining protein concentrations (Bio-Rad Protein Assay Kit), immunoblotting was performed ...
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No products found
because this supplier's products are not listed.
Pezhman Mohammadi, et al.,
bioRxiv - Developmental Biology 2019
Quote:
... in-gel tryptic digestion performed using proteoprofile trypsin in-gel digest kit (PP0100, Sigma-Aldrich). The samples were desalted using either ZipTip™ C18 (Millipore ...
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No products found
because this supplier's products are not listed.
Amir K. Foroushani, et al.,
bioRxiv - Systems Biology 2020
Quote:
... and protein was removed by digestion with 1.2 mg/mL proteinase K (Roche) in proteinase K buffer (50 mM Tris-HCl at pH 7.5 ...
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A component of the Papain Dissociation System, for use in the tissue dissociation method of...
Cat# LK003176,
1 vi, USD $25.0/1ea
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Somy Yoon, et al.,
bioRxiv - Molecular Biology 2019
Quote:
... The enzyme digestion was carried out by digestion buffer (calcium-free Tyrode buffer supplemented with hyaluronidase [Worthington, 0.1 mg·ml-1 ...
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No products found
because this supplier's products are not listed.
Christian Brion, Sheila Lutz, Frank W. Albert,
bioRxiv - Genetics 2020
Quote:
... We used ZR Fungal/Bacterial RNA mini-prep kit with DNase I digestion (Zymo Research R2014), following the kit manual ...
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No products found
because this supplier's products are not listed.
Orit David, et al.,
bioRxiv - Neuroscience 2018
Quote:
... Protein quantification was done using a BCA protein assay kit (GE Healthcare). Appropriate volumes of 2X SDS sample buffer (10% glycerol ...
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No products found
because this supplier's products are not listed.
Timothy Wessler, et al.,
bioRxiv - Immunology 2019
Quote:
... enzymatic digestion (Tumor dissociation kit, human; Miltenyi Biotec) was performed on excised granulomas using gentleMACS octo dissociator ...
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No products found
because this supplier's products are not listed.
Yuki Oiwa, et al.,
bioRxiv - Zoology 2020
Quote:
... The protein concentration was measured by TaKaRa BCA Protein Assay Kit (Takara Bio) in accordance with the manufacturer’s protocol ...
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No products found
because this supplier's products are not listed.
Quote:
... with proteinase K digestion (Dako, Glostrup, Denmark) used for antigen retrieval ...
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No products found
because this supplier's products are not listed.
Quote:
... The total protein concentration was determined by using Bradford protein assay kit (Abcam) and the collagen content of each sample was normalized to total protein.
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No products found
because this supplier's products are not listed.
Tamara M Sirey, et al.,
bioRxiv - Biochemistry 2018
Quote:
... Protein carbonylation was assessed using the OxyBlot protein oxidation detection kit (Merck Millipore), and differential carbonylation was assessed by densitometry ...
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No products found
because this supplier's products are not listed.
Quote:
... and protein purification kit (Macherey-Nagel 740609.240C) following the manufacturers protocol ...
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No products found
because this supplier's products are not listed.
Makoto Tanaka, et al.,
bioRxiv - Molecular Biology 2019
Quote:
... we extracted CY and PM proteins using the PM Protein Extraction Kit (BioVision). The protein samples were treated for 30 min at 50°C in a sample-treating solution containing 2% SDS and 5% β-mercaptoethanol ...
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No products found
because this supplier's products are not listed.
Tom Beneke, et al.,
bioRxiv - Cell Biology 2019
Quote:
Protein samples were prepared using filter-aided sample preparation (FASP) digestion (Wisniewski et al., 2009) (Vicacon500, Sartorius, VN01H02 10kDA). The FASP filter were washed with 200 µl 0.1 % trifluoroacetic acid (TFA ...
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No products found
because this supplier's products are not listed.
Jarrod J Sandow, et al.,
bioRxiv - Biochemistry 2019
Quote:
... Enzymatic digestion proceeded for 1 hr at 37 °C using the ThermoMixer C (Eppendorf) shaking at 400 rpm ...
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No products found
because this supplier's products are not listed.
Quote:
... Proteins were detected by immunoblotting with a chemiluminescence Luminol kit (SC-2048, Santa Cruz Biotechnology) using a LumiAnalyst Imager (Roche Applied Sciences).
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No products found
because this supplier's products are not listed.
Quote:
... Plant RNA kit with DNA digestion on column (Biotek, China) according to the manufacturer’s instructions ...
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No products found
because this supplier's products are not listed.
Ksenija Radic Shechter, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... through digestion of mammary glands in 5 mL of digestion media (Lonza/Amaxa DMEM/F12 1:1 Mixture with HEPES ...
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No products found
because this supplier's products are not listed.
Quote:
... recombinant proteins to a proprietary Ral effector protein was assessed using the RalA G-LISA™ Activation Assay Kit (#BK129, Cytoskeleton, Inc. Denver, CO), as per the manufacturer’s protocol ...
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No products found
because this supplier's products are not listed.
Quote:
The effect of KHS101 on HSPD1 mediated protein folding was assessed using the human HSP60/HSP10 protein refolding kit (R&D Systems, Cat # K-300) following the manufacturer’s instructions (no unspecific effect of KHS101 on the luminescence signal of client protein in absence of heat-induced protein denaturation was observed).
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No products found
because this supplier's products are not listed.
Justin Parreno, et al.,
bioRxiv - Cell Biology 2019
Quote:
... Total protein of samples loaded into capillaries was determined using a Total Protein Separation module kit (Protein Simple). Protein expression of specific molecules were normalized to total protein.
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No products found
because this supplier's products are not listed.
Mariana G Bego, et al.,
bioRxiv - Immunology 2019
Quote:
... ILT7 purified protein was labeled using the Protein Labeling Kit RED-NHS (NanoTemper Technologies). The labeling reaction was performed according to the manufacturer’s instructions ...
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No products found
because this supplier's products are not listed.
Babayemi O. Oladejo, et al.,
bioRxiv - Microbiology 2018
Quote:
... After dialysis and thrombin digestion (3 U/mg NA; BD Biosciences) overnight at 4°C ...
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No products found
because this supplier's products are not listed.
Quote:
... the immunoreactive protein was visualized using the Vectastain ABC kit (Vector Laboratories) and 3,3’ diaminobenzidine tetrahydrochloride hydrate (Sigma ...
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No products found
because this supplier's products are not listed.
Paweena Chaoprasid, et al.,
bioRxiv - Biochemistry 2020
Quote:
... proteins were visualized using the Western Lightning ECL II Kit (Perkin Elmer) and exposed on X-Ray film (GE Healthcare Amersham Hyperfilm ECL ...
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No products found
because this supplier's products are not listed.
Quote:
... The protein complexes bound to the beads were digested using ProteoExtract All-in-One Trypsin Digestion Kit (Cat. No. 650212, Calbiochem) according to manufacturer’s instructions ...
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No products found
because this supplier's products are not listed.
Quote:
... proteins were extracted using the ISOLATE II RNA/DNA/Protein Kit (BIOLINE). Protein concentration was determined using the MicroBCA Protein Assay Kit (Pierce-Thermo Scientific) ...
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No products found
because this supplier's products are not listed.
Lin Yuan, et al.,
bioRxiv - Immunology 2019
Quote:
... Protein concentration was measured by BCA protein determination kit (PC0020, Solarbio, China). Immunoprecipitations were performed overnight using the appropriate dilution of a polyclonal anti-ITGB4 antibody (Ab197772 ...
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No products found
because this supplier's products are not listed.
Qinlin Shi, et al.,
bioRxiv - Immunology 2020
Quote:
... and the protein concentration was measured by the BCA protein assay kit (Genscript, Nanjing, China) as previous described25.
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No products found
because this supplier's products are not listed.
Quote:
The p3E vectors were built by digestion of the p3E-MCS (Addgene #75174) with SalI/BamHI to clone a U6c:gRNA#1 sequence or by SpeI/SacII to clone U6d:gRNA#3 ...
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No products found
because this supplier's products are not listed.
Alejandra Arias-Cavieres, et al.,
bioRxiv - Neuroscience 2019
Quote:
... Protein content was determined using a Protein Determination Kit (Cayman Chemical, Cat# 704002) and absorbance was measured at 595 nm using a Nanodrop 2000 spectrophotometer (Thermofisher ...
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No products found
because this supplier's products are not listed.
Xin Zhang, et al.,
bioRxiv - Biochemistry 2020
Quote:
... Protein concentration was measured using Bradford protein assay kit (Tiangen Biotech, Beijing Co., LTD, CN). The purity of the PM was determined by standard marker assays[32] ...
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No products found
because this supplier's products are not listed.
Vineeth Vengayil, Sunil Laxman,
bioRxiv - Biochemistry 2019
Quote:
... The concentrations of these proteins were estimated by BCA protein estimation method (BCA assay kit, G-Biosciences). The in vitro ubiquitination assay was set up with the following components in the reaction mixture-5 nM Uba1p ...
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No products found
because this supplier's products are not listed.
Nehemiah S Alvarez,
bioRxiv - Molecular Biology 2019
Quote:
... 30 µL magnetic protein G beads (SimpleChIP Kit #9003, Cell Signaling Technology) were added to the samples and incubated at 4 °C with end over end rotation for 4 hours ...
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No products found
because this supplier's products are not listed.
May Daher, et al.,
bioRxiv - Immunology 2020
Quote:
... Cas9 protein (PNA bio) and gRNA were incubated at room temperature for 15 min in a 3:1 reaction with 15μg Cas9 and 5000 ng gRNA (2500ng of sgRNA#1 and 2500ng of sgRNA#2).47 The incubation product (gRNA and Cas9 complex ...
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No products found
because this supplier's products are not listed.
Quote:
... The Pst I restriction enzyme was used for sequence digestion and DNA was sequenced on the Illumina HiSeq 2500 platform (Illumina Inc., USA).
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No products found
because this supplier's products are not listed.
Alyssa Kent, et al.,
bioRxiv - Systems Biology 2020
Quote:
... Protein and crosslink digestion was carried out by adding 50 μL freshly prepared 20 mg/mL Proteinase K (VWR, freeze-dried powder suspended in 10 mM Tris ...
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No products found
because this supplier's products are not listed.
Quote:
... Immunoprecipitation was performed using crosslinked protein and a GFP-Trap®_A Kit (Chromotek) following the manufacturer’s instructions ...
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No products found
because this supplier's products are not listed.
Takehisa Noguchi, et al.,
bioRxiv - Pharmacology and Toxicology 2020
Quote:
Nuclear proteins were isolated from RAW264 cells using Nuclear Extract Kit (Active Motif, Carlsbad, CA, USA) according to the manufacturer’s protocols ...
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No products found
because this supplier's products are not listed.
Nadiia Rawlings, et al.,
bioRxiv - Cell Biology 2019
Quote:
... Total protein on membranes was stained using REVERT total protein stain (LI-COR). Immune complexes were detected using HRP-conjugated secondary antibodies (Sigma ...
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No products found
because this supplier's products are not listed.
Aran Singanayagam, et al.,
bioRxiv - Immunology 2019
Quote:
... Type III IFN proteins were measured using a custom-designed LEGENDplex kit (BioLegend). IFN-β was measured using the Verikine ELISA (PBL) ...
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No products found
because this supplier's products are not listed.
Jeonghwan Youk, et al.,
bioRxiv - Cell Biology 2020
Quote:
... followed by further dissociation using pre-warmed digestion buffer containing 2U/ mL Dispase II (Sigma, Corning), 1 mg/mL Dispase/Collagenase (Sigma ...
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No products found
because this supplier's products are not listed.
Kensuke Ninomiya, et al.,
bioRxiv - Molecular Biology 2019
Quote:
... All MS/MS spectra were searched against protein sequences of the RefSeq (NCBI) human protein database (RDB) using the Protein Pilot software package (AB Sciex), and decoy sequences were then selected with a false discovery rate <1%.
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No products found
because this supplier's products are not listed.
Victoria O. Pokusaeva, et al.,
bioRxiv - Genomics 2019
Quote:
... Non circularized templates were eliminated by 2 h digestion at 37°C with Plasmid-safe DNAse (Epicentre, #E3101K), the enzyme was inactivated by heating for 30 minutes at 70°C ...
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No products found
because this supplier's products are not listed.
Quote:
... Total protein was extracted using PEB protein extraction buffer (Agrisera Antibodies) according to the manufacturer‘s instructions ...
![OSI 930 - CAS [728033-96-3], >99%](https://image.labshake.com/supplier-product/305cc4ba-a97e-4815-88b6-a95430b03ef4/45612fdf-fc85-4cf4-b930-a7e7f23bd699.png)
c-Kit and VEGFR2 tyrosine kinase inhibitor. Sold for research purposes only.Cat# 1547.0, SKU# 1547-25, 25 mg, US $352 / EA, EURO €320 / EA Ask